• Korean Journal of Medicinal Crop Science
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• v.8 no.1
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• pp.9-13
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• 2000

To determine the proper carbon and nitrogen sources and their proper levels for mass micro propagation of Scrophularia buergeriana Miquel, tonic and curing cough experiment were applied and a method for mass cultivation by using bioreactors (2.5 L) was expinined. Proper ratio of $NH_4NO_3\;:\;$KNO_3$ was 413 mg/L : 1900 mg/L for multiple shoot production. Sucrose was more effective than glucose or fractose as carbon source and 3% concentration was good for shoot formation. Total nitrogen was not detected after six weeks both in 500 ml flask and bioreactor culture. Sucrose was decreased sharply after two weeks and there was no sucrose left after three weeks both in 500 ml flask and bioreactor culture. The stirrer in bioreactor caused shear stress to shoots severely. The sphere type bioreactor was better than the cylinder type and removal of inner loop in sphere type was more effective to avoid shear stress.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.168-169
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• 2003

현재 참외는 생산량의 대부분이 생과로 소비되고 있으나, 저장성이 낮아 새로운 가공식품 개발이 요구되고 있는 실정이다. 이에 참외식초/참외농축액을 함유하는 참외농축음료를 제조하였으며, 유통기간 동안 품질변화를 알아보기 위해 저장온도 및 저장시간에 따른 품질변화를 조사하였다. 배합조건에 따라 배합한 참외농축음료를 8$0^{\circ}C$에서 30분간 살균한 후 저장온도 및 시간에 따른 품질변화 및 관능적 특성변화를 모니터링하였다. 저장온도(20, 30, 40, 50, 6$0^{\circ}C$) 및 저장시간(0, 2, 4, 5, 8 week)을 독립변수(Xi.)로 하여 달리한 각 실험조건을 -2, -1, 0, 1, 2의 다신 단계로 부호화하고 실험조건을 설계한 다음 각 조건에서 저장된 참외음료의 품질특성(종속변수, Yn)을 모니터링하였다. 관능검사는 선정된 패널요원을 대상으로 4$^{\circ}C$에서 저장한 대조구와의 색상, 향, 맛 및 전반적인 기호도가 어떻게 달라지는가를 평가하였으며, 색도 및 갈색도를 측정하였다. 또한 제조된 참외 농축 음료를 저장하면서 미생물의 생육유무를 확인하기 위해 Petrifilm을 사용하여 미생물의 유무를 확인하였다. 참외식초/참외농축액을 이용한 참외농축음료를 제조하여 음료의 저장온도 및 저장시간에 따른 관능적인 특성 및 품질 특성을 모니터링하면서 저장성 실험을 통해 저장안전성을 평가한 결과 저장시간 및 저장온도에 따른 관능적인 색상은 1.6~4.0의 값을 나타내었으며, 향은 2.3~4.0의 값을 나타내었으며, 맛은 1.8~4.0의 값을, 전반적인 기호도는 2.1~4.0의 감을 나타내는 것으로 나타났다. 이화학적 품질 검사 결과, 색도L(백색도)값은 21.79~87.23, 색도 a(적색도)값은 -1.27~35.79의 값을 나타내었으며, 색도 b(황색도)값은 16.57~18.89의 값을 나타내었다. 갈색도는 0.386~3.214의 값을 나타내었으며, PH는 4.02~4.24의 값을 나타내었다. 일반미생물은 검사 결과 모든 조건에서 검출되지 않았다. 참외농축음료를 8$0^{\circ}C$에서 30분간 살균하여 미생물학적 품질 검사 결과 저장온도 및 저장시간에 따라 미생물이 검출되지 않았으며, 유통하기에 적합한 음료가 제조됨을 검증할 수 있었다.

• Journal of Plant Biotechnology
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• v.29 no.1
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• pp.51-57
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• 2002

For the plant regeneration of soybean (Glycine me L. Merr.), the shoot formation rate, optimal medium and tissue conditions were examined using Korean soybean cultivars. Among the parts of seedling, a node that includes one cotyledon showed the highest shoot formation rate among other tissues. Half-strength B5 medium was more efficient than full strength medium. Formation rates of pair shoots (1 to 2 shooting) were higher in the when benzyl adenine was supplemented. The formation rates of multiple shoots, that is, 4 to 5 in shooting, were high when thidiazuron was supplemented. Multiple shoot was de novo formed in cutting side of cotyledonary node. The effective concentration of thidiazuron for shoot induction treatment was 2 mg/L. Among the 27 cultivars, multiple shoot formation rates were high in the 11 cultivars including 'Heugcheongkong, and pair shoot formation rates were high in the 16 cultivars including 'Malikong'.

• The Journal of the Convergence on Culture Technology
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• v.4 no.4
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• pp.331-335
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• 2018

Zantedeschia spp. calla is very popular as a cut flower. It is very important to establish a micro propagation system through plant tissue culture with the problem that colored calla with various colors are low in natural reproduction rate and vulnerable to high temperature. In this study, we conducted the experiment by adding taurine to improve the growth of calla plant. When 20 mg/L of taurine was added with plant growth effect, 54.0 % of the cases of multiple shoots and 17.2 times of fresh weight were the most effective. Taurine 20 mg/L treatment showed 16.0 % and 39.2 %, respectively, than the untreated control. Taurine may contribute to mass propagation of elite breeding lines as well as an improvement of farm income by positively influencing the overall growth of calla plants, thereby positively affecting the establishment of the micro propagation system of calla shoot tips.

• Journal of Plant Biotechnology
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• v.34 no.3
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• pp.263-269
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• 2007

To establish a optimum culture condition in vitro for production of seedlings in Wasabia japonica, the effects of salt strength, sucrose concentration, and $NH_4/NO_3$ ratio on the shoot growth in MS medium were investigated. The full strength of MS medium was the best condition for shoot growth, and in $1/4{\sim}1/2x$ or 2x MS medium the growth was significantly suppressed. Growth of shoots was enhanced when sucrose was added in MS medium after 2 weeks of culture, and the best result was found at 3% sucrose addition. ${NH_4}^+/{NO_3}^-$ ratio of 10:50 gave the highest biomass of shoot in the liquid culture with MS medium, and increasing the ${NH_4}^+$ ratio in the medium significantly reduced the growth of shoot.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2002.04b
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• pp.33-40
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• 2002

We, Tong Yang Moolsan Co. Ltd. (TYM) set up the mass-production system for virus-free seed garlic via tissue culture technique. TYM's tissue culture technique is called as 'Multiple shoot propagation technique'. This technique can lead mass propagation of genetically homogeneous seed garlic in a short period because of its highly proliferation ,ate of in vitro shoots $(15^{10}/year)$. TYM researchers applied the technique to some selected garlic cultivars with superior characteristics and carried out Held test of productivity in the inside and outside of the country for several years. According to the yearly results of Held test with virus-free seed garlic, we ascertained that virus-free seed garlic can produce the highly yield increase (max. above 50%) and also can enhance the product quality. Consequently, we estimated that TYM's seed garlic will contribute to farmers with increase of income and can elevate the national position of garlic market in the world for its competitive power of technical and production cost.

• Journal of Plant Biotechnology
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• v.29 no.3
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• pp.171-177
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• 2002

We, Tong Yang Moolsan Co. Ltd. (TYM) set up the mass-production system for virus-free seed garlic via tissue culture technique. TYM's tissue culture technique is called as 'Multiple shoot propagation technique' This technique can lead mass propagation of genetically homogeneous seed garlic in a short period because of its highly proliferation rate of in vitro shoots (15/sup 10//year). TYM researchers applied the technique to some selected garlic cultivars with superior characteristics and carried out field test of productivity in the inside and outside of the country for several years. According to the yearly results of field test with virus-free seed garlic, we ascertained that virus-free seed garlic can produce the highly yield increase (max. above 50%) and also can enhance the product quality. Consequently, we estimated that TYM's seed garlic will contribute to farmers with increase of income and can elevate the national position of garlic market in the world for its competitive power of technical and production cost.

• Korean Journal of Heritage: History & Science
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• v.54 no.4
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• pp.212-231
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• 2021

This study examines the types of gods worshiped in the homes of Utsunoya village, the places where they are enshrined, the rituals and food offered to the gods, the decorations during the rituals, and the people's beliefs. Also, by comparing the gods worshiped in houses in remote Utsunoya with those of downtown Shizuoka, the differences and changes in the gods worshiped in the two contemporary spaces were predicted. Today, the gods enshrined in Utsunoya's houses are amatelaseu oomikami (天照大神), ancestor, ebisu, daigoku, kojin, inari, the god of the toilet, the god of land, and the god of water. From December 31st to January 3rd and on January 15th, Obon (July 15th), October when there is a festival at the village shrine, and on Ebisu Day (October 19th and 20th), residents offer drinks and food to the gods. Japanese beliefs at home are polytheistic in nature. They maintain national identity through kamidana and maintain family identity through ancestor worship linked to the Buddhist altar. The Japanese beliefs at home are firmly established in the background of the home, the base of family life. Japanese houses have a strong character as a religious space where they coexist "with the gods," and the residents have a cultural tradition of living with the gods.

• The Journal of the Convergence on Culture Technology
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• v.4 no.4
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• pp.325-329
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• 2018

There are two kinds of calla belonging to the Zantedeschia spp. depending on the growing condition. Between them, various colored calla with flower shape and flower color are very popular as cut flowers. However, colored calla is very low in natural reproduction rate, and it takes a long time to obtain a flowering bulb. So it is urgent to establish a large breeding system of good varieties. In this study, various plant growth hormone treatments were carried out to improve the growth of the plants in calla. Root formation was the highest at 81.5 % in basal MS medium, and the formation of shoot and multiple shoots were the highest at 100.0 % and 36.4 % in the combined treatment of BA 2.0 mg/L and IBA 1.0 mg/L, respectively. In fresh weight, the highest growth rate was observed with 11.2 times increase in BA 2.0 mg/L single treatment. Auxin and cytokinin mixed treatment were widely used in previous studies and positive effects on the growth of calla plants will help to establish the micro-propagation system.

• SUVANNABHUMI
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• v.1 no.2
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• pp.43-71
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• 2009

베트남 소수민족 공동체 중 Hmong족은 중요한 구성원이라고 여겨져 왔다. 타지에서 베트남으로 이민 와서 다른 민족들과 함께 오랫동안 살아 왔지만 Hmong족은 자기의 민족문화 특색을 잘 지켜왔다. 그런데 지난 몇 년 동안 전통적인 종교와 달리 새로운 종교를 믿게 되는 방향으로 Hmong족의 심령의 종교는 많이 변하고 있다. 그 중에 특히 주목받은 것은 개신교의 유입이다. 개신교는 직접적, 간접적 선교 방법으로 Lao Cai에 있는 Hmong족 공동체에 자연스럽게 유입되었다. Lao Cai의 많은 지방에서 사람들이 개신교를 믿게 되었고 그 중에는 가족이나 종족 환경에서 강제적 선교도 일어났다. 개신교는 Hmong족의 전통문화뿐만 아니라 Hmong족의 전통 종교, 신앙에도 직접적으로 영향을 주었다. 이 글에서는 Hmong족이 살고 있는 Lao Cai 지방을 선택하여 고찰하며 Hmong족의 전통문화가치에 대한 개신교의 영향 및 원인을 살펴보기로 한다. 먼저 Hmong사람들은 전통신앙을 버리고 개신교를 믿게 되는 원인을 살펴보자. 주 원인은 다음과 같은 네 가지가 있다고 본다. -경제와 관련된 원인 -성별 불평등과 관련된 원인 -학문 수준과 정보 습득 희망과 관련된 원인 -전통문화, 심리와 민속의식과 관련된 원인 그리고 다음과 같은 두 가지 각도에서 전통문화 가치에 대한 영향을 알아보았다. 첫째는 개신교가 Hmong사람의 전통사회체제에 큰 영향을 준 점, 둘째는 개신교가 Hmong사람의 풍속습관 및 전통 신앙에 영향을 준 점이다. 개신교가 Hmong사람의 전통사회체제에 큰 영향을 준 점은 여러 방면에서 보인다. 전통적인 가치와 개신교의 새로운 규정 사이의 충돌도 있고 가족 안에서 부부의 위치상 충돌도 있다. 또한 Hmong사람들에게 전통 종족의 역할, 위치를 약화시키는 영향도 주었고 산골 마을에서 개신교를 믿는 사람들과 믿지 않은 사람들 사이에 일어난 새로운 충돌도 있다 개신교와 개신교의 교리는 역시 풍속습관과 전통신앙에 큰 영향을 주었다. 첫째는 예수의 역할이 가장 중요하고 조상 숭배나 "집에 있는 귀신 숭배"같은 것보다 강력한 것이다. 그래서 Hmong사람의 신앙, 종교는 원시적 다신교부터 일신교로 변한 것으로 보인다. 둘째는 개신교를 믿는 사람들이 Hmong족의 전통 민속축제를 점차 멀리한다. 어떤 경우에는 그 사람들이 자기 민족의 전통문화 환경을 떠나 버리는 일도 있었다. 셋째는 개신교의 교리가 Hmong족의 풍속 규정을 바꾸게 하였고 Hmong사람들이 새로운 종교의 교리를 집행시켰다. 넷째는 인간 인생 주기에 관한 풍속습관 및 민속의례들은 의례 대상보다 의례 주제를 중심으로 삼는 것으로 점차 변화했다. 대체로 개신교가 Hmong족 공동체에 유입하여 이 민족의 전통문화 가치는 변화했다. Hmong족 공동체에 오래 존재해 왔던 낙후한 습관을 개선하는 점에서 개신교는 적극적 역할을 했다. 현재 문제점은 어떻게 전통문화가치를 유지하고 새로운 종교와 같이 존재할 수 있느냐라는 문제이다. 이 문제는 다음 연구 대상이 될 것이다.