• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.57 no.3
• /
• pp.310-315
• /
• 2012

The vegetable perilla is proved to be a late-maturing plant that flowers at the early of Oct. regardless of sowing time, so that the sowing time for seed production should be decided under consideration of maturity before beginning of frost. This experiment was carried out to determine the sowing date for seed production at greenhouse on late-maturing perilla cultivar, 'Ipdlkkae 1' in the middle region of Korea. The sowing dates were 8 times from May 6 to July 15 with an intervals of 10 days. As sowing date was delayed, the stem height, no. of nodes, no. of branches, no. of cluster per plant and no. of capsules per cluster were decreased. But as sowing was early, the lodging was occurred because of heavier growing. Days to flowering was linearly decreased about 0.86 day as affected by a day's delayed. But days from flowering to maturing was not significantly affected by sowing date. The grain yield was not significantly different among sowing from May 6 to June 15 and rapidly decreased the sowing after June 25 because of the reductions of no. of cluster and percent of ripened grain. Considering accumulative temperature, lodging, germination rate and grain yield, it is suggested that the sowing for seed production in late-maturing perilla cultivar should be finish before June 15 (transplanted at July 15) at greenhouse in the middle region of Korea.

• Reproductive and Developmental Biology
• /
• v.32 no.3
• /
• pp.159-166
• /
• 2008

We report here the generation of transgenic chickens that produce human Thrombopoietin (hTPO) using replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G). For the retrovirus vectors, we used hCMV (human Cytomegalovirus) internal promoter to drive the hTPO gene. After confirming the expression of the hTPO gene in various target cells, the concentrated solution of recombinant retrovirus was injected beneath the blastoderm of non-incubated chicken embryos (stage X). The biological activity of the recombinant hTPO in target cell was significantly higher than its commercially available counterpart. Out of 132 injected eggs, 11 chicks hatched after 21 days of incubation and 4 hatched chicks were found to express vector-encoded hTPO gene. However, 3 out of the 4 transgenics died within one month of hatching. The major significance of this study is that it is one of the very few successful reports on the production of transgenic chickens as bioreactors aiming mass production of commercially valuable and biological active human cytokine proteins.

• The Korean Journal of Pesticide Science
• /
• v.10 no.3
• /
• pp.183-188
• /
• 2006

A nation-wide monitoring survey was performed to look into pesticide use pattern and amount on farmers' field after the national registration of pesticides from 2003 to 2004. Tow hundred ninety two leading farmers involved in the survey were chosen from main cultivating regions of targeted crops in consideration with area. Most farmers involved in the survey follow the safe use guideline of pesticides, while their information to select and making-decision for purchasing pesticides were done according to the recommendation of mainly pesticide market dealers or agricultural cooperative dealers. Used amount of pesticide per unit cropping area was surveyed as 5.51 on paddy, 3.91 on field cabbage, 2.93 on greenhouse cabbage, 0.76 on lettuce, 0.55 on spinach, 1.34 on perilla leaf, 4.89 on leek and 0.42 kg $ha^{-1}$ on young radish. As compound with those surveyed from 1999 to 2000, the pesticide amounts used for rice, lettuce and perilla leaf were reduced while that for cabbage was increased.

• The Korean Journal of Pesticide Science
• /
• v.15 no.3
• /
• pp.303-309
• /
• 2011

This study was carried out to investigate the evaluation of insecticidal efficacy and control effect in field of 10 environmental-friendly agricultural materials (EFAM) and seasonal occurrence at the vineyards to the Lycorma delicatula. L. delicatula nymphs occurred from late May to late August the highest early June (1st nymph) while adults occurred from late July to November with the highest peak of early October (preovipositing female) in 2010. Total population density of L. delicatula was the highest in early June. For the 3rd~4th nymphal instars and adults, EFAM (Spider, Jindikap-plus and Byejin-${\alpha}$) showed perfect insecticidal activity 2hour after treatment. Seoncho showed insecticidal activities 96.7% within 48 hour. Residual effects between EFAM showed 55.5% Spider at recommended concentration at 7 days after treatment(DAT), the other EFAM had low efficacy. Jindikap-plus and Spider showed 99% control value on nymphs in the field test at 3DAT, appeared control value more than 90% at 7DAT. Also, the control effects of Jindikap-plus and Spider were showed the adult mortality of 100% and 98.2% respectively at 3DAT, 90.6% and 84.6% respectively at 7DAT. Whereas the other EFAM had low efficacy. Jindikap-plus and Spider were excellent control effects compared with Acetamiprid WP.

• The Korean Journal of Pesticide Science
• /
• v.16 no.4
• /
• pp.395-400
• /
• 2012

In order to survey the actual pesticide usage, this survey was carried out on the growers of 3 plant groups, fruit vegetables, fruits and rice field, from 2009 to 2011. 390 farmers were selected from main production areas to investigate their pesticide application during the growing seasons. Input amounts per area of domestic fruit vegetables was surveyed as 11.6 kg a.i./ha for red pepper of field culture, 2.0 kg a.i./ha for red pepper of greenhouse, 2.3 kg a.i./ha for strawberry, 2.2 kg a.i./ha for watermelon, 4.8 kg a.i./ha for cucumber, 1.5 kg a.i./ha for oriental melon and 2.1 kg a.i./ha for tomato, respectively. Input amounts per area on fruits and on rice field were surveyed as 11.7 kg a.i./ha for apple, 12.5 kg a.i./ha for pear, 7.7 kg a.i./ha for peach, 4.5 kg a.i./ha for grape, 40.0 kg a.i./ha for citrus 5.4 kg a.i./ha for persimmon, and 3.46 kg a.i./ha for rice, respectively. As a result of the actual pesticide usage survey, The safety guideline for pesticide use is generally kept well by all farmers of fruits vegetables, fruits and rice.

• Research in Plant Disease
• /
• v.15 no.3
• /
• pp.193-197
• /
• 2009

Pseudocercospora leaf spot was major disease of grape cultivar 'Campbell Early' in Korea. Leaf spot first appeared in early June and rapidly dispersed to other leaves through rainy season. Disease progress of leaf spot by Pseudocercospora vitis in plastic green house, in the two provinces (Gimje and Gimcheon), were investigated in 2007. Differences of Infected leaves (%) between cultivation systems were observed in field and plastic green house, but there was no difference between provinces. Micro environmental factors, such as temperature and relative humidity, were correlated with infected leaves by PROC REG procedure of SAS (Statistical Analysis System). As a result, regression model best described ($R^2=0.95^{**}$) the infected leaves as a function of the interaction of cumulated temperatures; Y (Infected leaves)=-7.0101+0.0496$\times$20Hcum (Cumulated hour above 20 degree)+0.0208$\times$20cum (Cumulated temperature above 20 degree)-0.2781$\times$25Hcum (Cumulated hour above 25 degree). A statistics model was shown that cumulated hour and temperature above specific degree were critical factor for Pseudocercospora leaf spot on the grapevine leaves in plastic green house.

• Proceedings of the Korean Society of Organic Agriculture Conference
• /
• 2009.12a
• /
• pp.304-305
• /
• 2009

시설재배지의 토양화학성 변화는 작물재배 기간 시비한 화학비료에서 유래된 무기성분 뿐만아니라, 가축분퇴비의 질소성분의 토양잔류량이 요소비료 보다 9.4배 많아 염류집적 주 요인이라는 보고('05 경기도)가 시사하는 봐와 같이 유기자원으로 시용하는 가축분 등의 부산물비료의 무기화에서 유래된 비료성분이 토양염류집적 및 토양환경악화에 더 큰 영향을 미칠 수 있다. 시설재배지의 유기물자원 시용기준이 토양의 특성에 관계 없이 작물에 따라 양적인 시험성적이 주로되어 있으며, 토양검정에 의한 시용기준도 유기물함량에 따라 볏짚, 우분, 돈분 및 계분으로 돠어 있다. 일반노지와 달리 시설재배지에서는 유기물함량이 토양의 비옥도 및 작물생육에 영향을 미치는 것 보다는 토양의 전기전도도(EC)가 더 중요한 작물생육 조건이 될 수 있다. 따라서 토양의 특성에 따라 물질순환에 의한 유기자원 시용기준으로 개선할 필요성이 있다. 시설재배지의 장기적인 토양관리를 위하여 유기물자원에 의한 토양환경 개선 효과를 구명하고자. 무처리, 가축분부산 물비료 관행 시용 기준 대비 볏짚 등 5개의 유기자원을 토양의 무기태질소 함량 대비 유기자원의 탄소함량을 C/N율 10 조절량을 시용하여 시험하였고, 또한 토양의 전기전도도(EC)가 상이한 3개( <2.0 dS/m, 2.0~6.0 dS/m, 6.0 dS/m<)토양에 유기물자원(우드칩)을 C/N율 10, 20, 30 조절하여 수박을 시험작물로 비닐하우스에서 재배하여 수행하였다. 시험 후 토양의 전기전도도(EC)는 시험 전에 비하여 시험 후 토양에서 가축분부산물비료는7% 증가되었으나 유기물자원 처리는 26~33% 경감되는 효과가 있었다. 수박의 과중은 무처리를 제외하고 처리간에 차이가 없었다. 유기물자원 C/N율 조절간에는 시험전 토양의 EC에 따라 차이가 있어 C/N 10 조절에서는 26~44%, C/N 20 조절에서는 30~51%, C/N 30 조절에서는 27~48% 경감효과가 있었으며, 3토양의 평균 토양EC 경감율은 C/N 10, 20, 30 조절에서 각각 34, 39 및 38 % 이었다. 수박의 생육 및 과중은 토양의 C/N율 조절간에는 차이가 없었으나, 토양의 EC 간에는 토양의 EC가 6.0dS/m 이상 토양에서 가장 낮았다. 따라서 탄소원의 유기자원을 C/N율 조절에 의한 시용기준 개선으로 토양의 무기태질소와 토양의 전기전도도(EC)를 경감시켜 친환경적 토양관리와 수박의 수량과 품질을 향상시킬 수 있을 것으로 평가되었다.

• Korean Journal of Organic Agriculture
• /
• v.17 no.4
• /
• pp.501-517
• /
• 2009

This research was carried out to study the effect of mulching materials and weed control methods on weed occurrence and growth of kale on upland and paddy soil of field culture at spring and autumn season. Paddy soil temperature of mulching treatments was high by $0.9{\sim}2.0^{\circ}C$ in comparison of non-mulching at autumn season. The control value of weed was over 91% at mulch paper and time required for weed control was reduced by 70% at mulch paper + machine weeding in comparison of non-mulching + hand weeding. The yield of kale was similar to that of conventional culture. Paddy soil temperature of mulch paper was higher on April, but lower on May and June than non-mulching at spring season. Dominant weed was Chenopodium album var. centrorubrum Makino, The control value of weed was over 91% at mulch paper, time required for weed control was reduced by 75% at mulch paper + machine weeding in comparison of non-mulching + hand weeding and the yield of kale increased by 34% than conventional culture. Dominant weed was Alopecurus aequalis var. amurensis Ohwi. on paddy soil at autumn season. The control value of weed was 43% at mulch paper. Time required for weed control was reduced by 80% at mulch paper + machine weeding in comparison of non-mulching + hand weeding. The yield of kale increased by 26% compared with than of conventional culture.

• Horticultural Science & Technology
• /
• v.32 no.6
• /
• pp.906-911
• /
• 2014

A new coreopsis cultivar, 'Uridream Pink', was developed by mutation breeding using gamma-ray irradiation. Rooted cuttings of 'Uridream 01', developed at Uriseed Company, were exposed to various gray ranges of gamma-rays from a $^{60}CO$ source for 24 h in 2009 at the Korea Atomic Energy Research Institute. Using gamma-rays with the range of 10-100 Gy, a chimeric mutant with pastel pink flowers was induced at 30 Gy from 'Uridream 01', which blooms red-purple flowers (Red-purple group, 59A). Clones that produced flowers with modified pink color were separated from the chimeric mutant and fixed by more than three rounds of cutting back from 2009 to 2010. The separated mutant clones with modified pink color were found to bloom flowers with marketable color and be free of any abnormal plant characteristics. The typical color of the flowers was pastel pink (Red-purple group, 67B), and the clones were registered as 'Uridream Pink' at the Korea Seed and Variety Service (plant variety protection number: 4410). Although the size of flowers and leaves of 'Uridream Pink' are smaller than those of 'Uridream 01', the number of flowers per plant is greater. 'Uridream Pink' can be used as both a pot and a garden plant because of its long blooming period from late spring to late autumn.

• Research in Plant Disease
• /
• v.8 no.1
• /
• pp.1-10
• /
• 2002

Climate in the year of 2001 could be summarized as severe drought from March to May, unusually high temperature in whole plant growth periods and clear weather condition especially in harvesting time of September and October without any typoons. In rice, major diseases such as leaf and panicle blast, bacterial blight, sheath blight and bacterial grain rot developed little due to unfavorable weather conditions, however, brown spot occurred severely due partly to the reduced use of N-fertilizer, Rice stripe virus infection was unusually high mainly in the west coast areas with four times more diseaseD area campared to the previous year, In pepper phytophthora blight was extremely severe in Cheonnam and Cheonbuk provinces, where had frequent rainfalls during growing period. Incidence of major diseases of tomato and cucumber in 2001 was relatively mild. In watermelon, penicillium fruit rot, one of the peculiar disease, spread over major production areas, whereas CGMMV, usually severe in every years was much reduced. Watermelon plants growing in open-fields were more severely diseased than those in plastic houses. Major diseases of chinese melon and strawberry occurred slightly and in particular, sudden wilt syndrome of chinese melon which was severe in 2000 showed mild infections in 2001. Incidence of white rot of garlic and onion was much lower unlike other years due mainly to spring drought and high temperature in the growing period. Infected area of potato bacterial wilt tended to increase in 2001 while fusarium wilt of sweetpotato was decreased. Apple diseases were generally mild, but powdery mildew of pear increased sharply. Barley scab was not severe as seen in other years.