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Effects of Shading and Mixing Ratio of Recycling Spawn bed Logs on the Yield of Gastrodia elata Blume (차광정도와 접종목 흔용이 천마의 수량에 미치는 영향)

  • Kwon, Young-Hwan;Lee, Jeong-Dong;Jung, Do-Chul;Yoon, Se-Jin;Hwang, Young-Hyun
    • Korean Journal of Plant Resources
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    • v.18 no.3
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    • pp.512-517
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    • 2005
  • To establish the economical cultivation method of Gastrodia elata Blume by controlling the shading rate and the mixing ratio of recycling spawn bed logs, a serial experiment was carried out. When G. elata was grown in the field with no shading, the yield of spawn tuber was higher than that of tuber of G. elata. The yield of G. elata was high in the rainproof green house covered with one layer of $95\%$ shading net, in the rainproof green house covered with two layers of $95\%$ shading net, and in the field with no shading, in that order. In the case of mixing ratio of recycling spawn bed logs, the yield was high in the fresh logs, in the mixing ratio of 2:1, and the ratio of 1:1, in that order. The income of the rainproof green house covered with one layer of $ 95\%$ shading net which brought the best yield was $81\%$ in the mixing ratio of 2:1 over the fresh logs.

Plant Regeneration via Multiple Shoots Formation from Sucker Explants of Rubus fruticosus L. (블랙베리(Rubus fruticosus L.)의 맹아절편체로부터 다경유도를 통한 식물체 재분화)

  • Shin Jeong-Sun;Sim Ock-Kyeong;Lee Jong-Chon;Cho Han-Jik;Kim Ee-Yup;Lee Kang-Seop
    • Korean Journal of Plant Resources
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    • v.18 no.3
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    • pp.456-461
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    • 2005
  • This study was carried out to induce plant regeneration via shoot formation from sucker explants of Rubus fruticosus L. To induce adventitious shoots, sucker explants were sterilized in $1.2\%$ NaOCl solution, and cultured on the MS solid medium supplemented with kinetin (0.5, 1.0, 3.0 mg/L) and BA (0.5, 1.0, 3.0 mg/L), respectively. As above, to induce adventitious shoots, sucker explants were cultured on the MS solid medium supplemented with IBA (0, 0.1, 1.0 mg/L) and BA (0, 0.1, 1.0, 2.0 mg/L). After 4 weeks of culture, the highest frquency $(100\%)$ of shoot formation from sucker explants was obtained from the medium with 1.0 mg/L BA. The highest shoot number per explant from in vitro shoot explants was 5.3. After 10 weeks of culture, the number of shoot per explant was increased. The highest frequency $(85\%)$ of root formation was obtained at 0.5 mg/L glycine medium, when the explant with shoot were cultured on the MS medium containing glycine at various concentrations from 0 to 2.0 mg/L. The survival rate of the plantlets after transfer to plastic pots containing sand, soil, and vermiculite (1:1:1, vol.) was $95\%$. The results indicate that micropropagation procedure can be applied for an efficient mass propagation of Rubus fruticosus.

Breeding of ‘Gommany’ Cultivar of Gomchwi with Disease Resistant, High Quality, and Yield (고품질 내병 다수성 곰취 신품종 ‘곰마니’ 육성)

  • Suh, Jong Taek;Yoo, Dong Lim;Kim, Ki Deog;Lee, Jong Nam;Kwon, Young Seok;Kim, Won Bae
    • Korean Journal of Plant Resources
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    • v.29 no.5
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    • pp.625-629
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    • 2016
  • A new Gomchwi cultivar ‘Gommany’ was bred by crossing between Gomchwi (Ligularia fischeri (Ledeb.) Turcz.) and Handaeri-gomchwi (Ligularia fischeri var. spiciformis Nakai). The selection and investigation of growth and yield characteristics were conducted from 2006 to 2011 in field and greenhouse of Highland Agriculture Research Institute, NICS, Rural Development Administration. The color of petiole ear was purple. Petiole trichome existed, but light of leaf back did not. Density of leaf vein was 5 degree in a newly developed cultivar ‘Gommany’. Plant height, leaf length, leaf width and petiole length were 47.7, 16.3, 21.2, and 31.4 ㎝, respectively in the 3 year of growth characteristics. Plant size was also higher than that of ‘Gondalbi’. Bolting and flowering time were Aug. 5 and Sept. 10, respectively. In contrast, Bolting time of ‘Gondalbi’ showed 25 days quite earlier bolting time compared to ‘Gommany’, Which led to earlier flowering time. ‘Gommany’ showed higher number of leaves (138) per plant compared to ‘Gondalbi’ (130). Furthermore, yield was 97% higher in ‘Gommany’ (1,575 g/plant) than in ‘Gondalbi’ (798 g/plant). ‘Gommany’ showed higher leaf thickness (0.69㎜) than ‘Gonalbi’ (0.46 ㎜), and consequently showed more hardness in leaf characteristics (24.3 ㎏/㎠) compared to ‘Gondalbi’ (23.0㎏/㎠). ‘Gommany’ showed higher resistance in the susceptibility of powdery mildew compared to ‘Gondalbi’. ‘Gommany’ cultivar was registered as a novel variety with number 90 in The Minister of the Korea Forest Service on December 2015.

Measurement of Exchangeable Cations in Salt Accumulated Vinyl Greenhouse Soils (염류집적 비닐하우스 토양의 교환성 양이온 측정)

  • Chung, Jong-Bae;Lee, Yong-Se;Jeong, Byeong-Ryong
    • Korean Journal of Environmental Agriculture
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    • v.37 no.1
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    • pp.21-27
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    • 2018
  • BACKGROUND: Although 1 M $NH_4OAc$ (pH 7.0) is predominantly used as the extractant of exchangeable cations in agricultural soils, this method is unsuitable for extracting the cations in saline and calcareous soils. This study was performed to select a proper method to determine exchangeable cations in vinyl greenhouse soils. METHODS AND RESULTS: Exchangeable cations (Ca, Mg, K, Na) in saline vinyl greenhouse soils were determined after extraction with 1 M $NH_4OAc$ (pH 7.0 and 8.5) and 1 M alcoholic $NH_4Cl$ (pH 8.5). Sum of exchangeable cations of the soils extracted with 1 M $NH_4OAc$ at pH 7.0 was 1.9-2.5 times greater than soil cation exchange capaity determined at pH 7.0, even though soluble salts were pre-removed. A similar result was found when the cations were extracted with 1 M $NH_4OAc$ at pH 8.5. Those results are mostly due to the overestimation of exchangeable Ca and Mg, linked to a partial dissolution of sparingly soluble salts in $NH_4OAc$ solution. When extracted with 1 M alcoholic $NH_4Cl$ at pH 8.5, extractable Ca and Mg decreased significantly due to the lower solubility of Ca and Mg carbonates in the extractant. And the sum of exchangeable cations was very close to the corresponding exchange capacity of soils. CONCLUSION: Alcoholic $NH_4Cl$ (pH 8.5) is proposed as a reliable extractant in determination of exchangeable cations in saline vinyl greenhouse soils. And soluble salts should be removed prior to the extraction of exchangeable cations.

Characteristics of Cucumber mosaic virus-VCH Causing Vein Chlorosis on Red Pepper in Korea (고추에 엽맥퇴록병을 일으키는 오이 모자이크 바이러스(CMV-VCH)의 특징)

  • Cho, Jeom-Deog;Lee, Sin-Ho;Kim, Jeong-Soo;Choi, Gug-Seoun;Kim, Hyun-Ran;Chung, Bong-Nam;Ryu, Ki-Hyun
    • Research in Plant Disease
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    • v.12 no.3
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    • pp.226-230
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    • 2006
  • Cucumber mosaic virus(CMV) was occurred on red pepper showing vein chlorosis or vein necrosis with the incidence rate of 52% from 62 specimens collected in natural fields. Among 32 samples infected with CMV, the specimens of 22 red pepper leaves showing vein chlorosis were infected singly with CMV-VCH. CMV-VCH induced vein chlorosis on the inoculated leaves, and vein banding and vein necrosis on the upper leaves of Nicotiana glutinosa, and then killed after showing bud necrosis. The typical symptoms of vein banding, malformation and blister were produced on the upper leaves of Nicotiana benthamiana and N. tabacum 'Ky-57' without symptoms on the inoculated leaves. The commercial cultivars of 'Bugang', 'Manitta' and 'Gwariput' were shown the typical symptom of vein chlorosis by the mechanical inoculation of CMV-VCH. CMV-VCH was detected specifically by RT-PCR. Virus particles of CMV-VCH were isometric shape having 30 nm diameter. Ultraviolet absorption of purified CMV-VCH was maximum at 260 nm and minimum at 242 nm. The ratio of A260/A280 was 1.71. CMV-VCH had the single nucleo-protein having the molecular weight of 24.5 kDa.

Inhibitory Effect of Cheese Whey on Cucumber Mosaic Virus and Pepper Mottle Virus in Capsicum annuum (치즈 유청의 오이모자이크바이러스와 고추모틀바이러스 감염 억제 효과)

  • Chung, Bong Nam;Kwon, Sun Jung;Choi, Gug Seoun;Yoon, Ju Yeon;Cho, In Sook
    • Research in Plant Disease
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    • v.26 no.2
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    • pp.103-108
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    • 2020
  • Evaluations were made for the effects of cheese whey treatment on infection of pepper plants by cucumber mosaic virus-Vch (CMV-Vch) and pepper mottle virus-Kr (PepMoV-Kr). In a greenhouse, pepper plants sprayed with whey, prior to inoculation by CMV-Vch using aphids, showed a viral infection rate significantly lower (6.6%) than for the control (23.3%). In an open field experiment, in which CMV infection relied on natural transmission by aphids, pepper plants were sprayed with undiluted whey once a week, starting on the transplanting date (May 2) to the end of June. On June 5, these whey-sprayed plants showed a CMV infection rate reduced by 18.9% and 16.7%, compared to untreated and pesticide-treated plants, respectively. In the greenhouse, pepper plants inoculated with PepMoV-Kr mixed with whey showed a viral infection rate decreased by 60% compared to the control. The accumulated amount of PepMoV-Kr coat protein was less than that for the virus-only control at 6 days post inoculation (dpi), but increased up to a similar level as the control at 9 dpi. This study showed that cheese whey is effective in reducing infection of both CMV and PepMoV in pepper plants.

The Phenomenological Study on the Male Immigrant Workers' Lives after Undergoing the Industrial Accidents (남성 이주노동자의 산업재해 후 삶에 대한 현상학적 연구)

  • Ro, Ji hyun
    • Korean Journal of Social Welfare
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    • v.68 no.1
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    • pp.23-52
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    • 2016
  • This study aims to reveal the meaning and essence of male immigrant workers' life who underwent industrial accidents through specific experiences. This study is based on the Van Manen(1990)'s lived experience phenomenological method, which actively describes the experiences about the industrial accidents in the perspective of male immigrant worker. The in-depth interviews were carried out with the thirteen male immigrant workers participants who underwent the industrial accidents. Through the interview, 121 meaning units and 38 disclosed themes were constructed. The following is the summarized results as 9 essential themes: < the oppression of the Industrial accident compensation insurance's hospitals to the aliens >, < being treated like the surplus man who lost the labor force >, < the class rank below despite undergoing the industrial accidents >, < survival having resistance sentiments >, < living at the anonymous lands as the Homo sacer >, < the stratified strategies between the immigrant workers >, < the origins as the bodiless shadow >, < struggling to escape the present conditions >, < present circumstances tied by the past experiences without hope >, researcher sought the essential meaning structure about the four Life-world Existentials (Body, The other, Space and Time) of constructing human's live-world. Based on the study results, some suggestions were made to restore the male immigrant workers' damaged quality of their lives who experienced industrial accidents and to contribute the social integration in view of the social welfare.

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Research Trends of Web-Based Inquiry Learning Effectiveness in Science Education: A Review of Publications in Selected Journals from 2000 to 2014 (과학교과 웹 기반 탐구학습의 효과성 연구 동향)

  • Lee, Jeongmin;Park, Hyunkyung;Jung, Yeonhwa;Noh, Jiyae
    • Journal of The Korean Association For Science Education
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    • v.35 no.4
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    • pp.565-572
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    • 2015
  • The purpose of this paper is to offer an analysis on the research trends of web-based inquiry learning effectiveness in science education, and to present suggestions for future studies. This study compiled data on 43 articles in Korea and international journals. The content analysis of articles published were from academic journals related to science education and educational technology from 2000 to 2014. The results are as follows: Among domestic articles, the participants ranged from school children to high school students. On the contrary, among foreign articles, the participants are centralized on secondary school students; most used experimental studies; most of the studies resulted with web-based inquiry learning in science education showing effectiveness on science learning performance or science inquiry ability; all web-based inquiry learning were designed using different models of teaching and learning, with the result in the case of domestic research, the utilized models refer to the STS learning model, Internet utilization problem-center inquiry learning model, Procedural model, while in the case of overseas research, the utilized models are SCY, IBLE, and TESI model. Implications of the findings are then discussed, which implies considerations for further research related to web-based inquiry learning.

Evaluation of the Farmers' Workload and Thermal Environments during Chili Harvest in the Open Field (여름철 노지 고추 수확 작업시 고령농업인의 온열 부담 평가)

  • Chae, Hyeseon;Kim, Hyunjin;Oh, Youngsoon;Lee, Kyungsuk;Kim, Hyocher;Kim, Kyungran
    • The Korean Journal of Community Living Science
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    • v.24 no.4
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    • pp.543-552
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    • 2013
  • Physiological and subjective responses of the farmers and thermal environment during chili harvest in the open field were investigated to evaluate the thermal environments and farmers's workload. Eight career female farmers in their sixties participated as subjects both in morning work(MW, AM 9:00~10:30) and in afternoon work(AW, PM 15:00~16:30) with each lasting about 90 minutes. The results were as follows. 1) Air temperature, air humidity, globe temperature and WBGT of MW were mean $25.54^{\circ}C$, 81.82%RH, $37.72^{\circ}C$, $26.27^{\circ}C$ and AW were mean $30.63^{\circ}C$ 82.50%RH, $40.11^{\circ}C$, $30.02^{\circ}C$, respectively. By the WBGT, we evaluated that the thermal environment in the afternoon in the open field gave a thermal burden to farmers. 2) Mean skin temperature of AW($34.8{\pm}0.8^{\circ}C$) was higher than MW($33.5{\pm}1.2^{\circ}C$)(p<0.05). Clothing microclimate temperature on the chest of each work time were $31.3^{\circ}C$(MW) and $32.7^{\circ}C$(AW). Clothing microclimate humidity on the chest of each work time were over 80%RH. Heart rate were 88.5bpm(MW) and 91.7bpm(AW) respectively. 3) Farmers working in the afternoon felt uncomfortable after 45~60 min. of work and in the morning they felt uncomfortable after 90 min. of work. We evaluated that the harvesting of chilies in the open field was 'moderate work' by the physiological responses but the level of thermal burden increased over time especially in the afternoon work. It is suggested that farm workers should drink fluids between work to stay in homeostasis by sweating and to take frequent rests. Active clothing ventilation and wearing functional garments would help farm workers excrete sweat effectively.

An Investigation into the Release of Chemical Oxygen Demand in Organic Filter Media (유기성 여재로부터 화학적 산소요구량 물질의 방출에 관한 연구)

  • Guerra, Heidi B.;Kim, Youngchul
    • Journal of Wetlands Research
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    • v.22 no.3
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    • pp.171-177
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    • 2020
  • To improve the nitrogen reduction capability of stormwater treatment systems subjected to intermittent saturation, organic materials are often added as filter media. However, these materials can be an additional source of organic carbon and increase the chemical oxygen demand (COD) in the outflow. In this study, different types of organic filter media were subjected to a batch leaching test to observe and quantify the release of COD. Results reveal that the initial pH of the tap water used for soaking which is 7.5-7.7 is conducive to the release of organics from the media to the leachate. The highest amount of COD released was observed in yard clippings and woodchip followed by compost and bark mulch. The leaching of organics also increased as the size of the media decreases due to higher surface area per volume. In addition, empirical regression analysis predicted that COD from these organic media will be exhausted from the material in 3-5 months to up to 26 months depending on the type of media. The results of this study can serve as a guide in estimating the potential release of COD in organic media in order to ensure their safe application in stormwater treatment facilities.