• Korean journal of applied entomology
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• v.60 no.2
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• pp.193-199
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• 2021

Solenopsis japonica, which is belonging to Formicidae in Hymenoptera, is a native ant species in Korea. However, it had not been studied for cold hardiness of S. japonica to understand on its overwintering mechanisms in field so far. Cold tolerance on developmental stages was measured at different cold temperature with various exposure times. Workers showed more survival at 5℃ and 10℃ compared with other stages and elevated cold tolerance when workers were exposed at 15℃ for more than 12h incubation as a rapid cold hardening (RCH) condition. RCH treatment not only increased survival of workers at cold temperatures, but also decreased supercooling point (SCP) and freezing point (FP). RCH group increased the survival rate by 44% at 10℃ compared with Non-RCH group. SCP and FP were depressed from -10.0 to -14.2℃ and from -11.3 to -15.3℃, respectively, after RCH treatment. Cold temperature increased expression level of cold- and stress-related genes such as glycerol kinase and heat shock protein. These results indicate unacclimated cold tolerance of S. japonica and its acclimation to low temperature by RCH.

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.5 no.9
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• pp.74-81
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• 1984

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.2
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• pp.167-176
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• 1999

Shoot morphological characteristics were affected by plant population densities in alfalfa. Shoots per plant, yield per shoot, yield per plant, and stem diameter increased with decreasing alfalfa population density. Winter survival was maximized at a plant population of $45plants\;m^{-2}$ or less. The grams of TNC per root increased as the stand density decreased. The lowest LT 50 occurred at the lowest stand density ($16plants\;m^{-2}$ having 25 cm plant spacing) and the highest LT 50 was attained at the highest stand density($494plants\;m^{-2}$ having 4.5 cm plant spacing). There were no differences in shoot morphology, winter survival, and cold hardiness in between 'Algonquin'and 'Vernal'. From this study, high stand density of alfalfa ($278plants\;m^{-2}$ or more) appears to be of little advantage compared to low stand density in terms of winter survival and cold hardiness.

• Journal of agriculture & life science
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• v.44 no.6
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• pp.61-67
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• 2010

Six evergreen ground-cover species including Ajuga reptans, Carex morrowii 'Variegata', Dianthus 'Night Star', Hedera helix 'Ivalace', Pachysandra terminalis, and Sedum rupestre, were tested for cold acclimation with three different light treatments (0, 30, and 70% of shading, respectively) to select potential candidates for over-wintering in central Korea. Among those species, Ajuga reptans, Carex morrowii 'Variegata', and Sedum rupestre performed well, which had more than 50% of survival rate in all light conditions. However, Dianthus 'Night Star' had less than 40% under any light treatment and the other two species, Hedera helix 'Ivalace' and Pachysandra terminalis, survived more than 40% only with 70% of shading condition. These results imply that those candidate species having less cold hardness in central Korea could have the potential to could over-winter as long as a proper light condition is provided.

• Horticultural Science & Technology
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• v.32 no.5
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• pp.590-599
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• 2014

This study was conducted to compare the cold tolerance of seven ground cover species, Orostachys japonica, Sedum oryzifolium, S. kamtschaticum 'SG1', S. reflexum, S. rupestre 'Blue Spruce', S. spurium 'Green Mental', and S. takesimense, which have been used for green roof and wall systems in Korea. Plants were grown in 10-cm pots and 1 g of tissues at stem-end and crown of each species were kept under either light or dark condition, respectively. For cold tolerance tests, plants were initially left at $4^{\circ}C$ and linearly cooled to 0, -4, -8, -12, -16, and $-20^{\circ}C$ at $-2^{\circ}C{\cdot}h^{-1}$ rate. Low temperature injury and regrowth rates were visually evaluated and assessed by image analysis, respectively. The lethal temperature ($LT_{50}$) of plant species was determined using electrolyte leakage measurements. S. reflexum was the most cold tolerant, showing the most survival at $-16^{\circ}C$, whereas S. oryzifolium and S. takesimense showed low temperature injury at $-8^{\circ}C$. Similar results were found with electrolyte leakage measurements at the stem end. For each species, the crown (Mean $LT_{50}:\;-12.15^{\circ}C$) was more cold tolerant than the stem end (Mean $LT_{50}:\;-10.47^{\circ}C$). In conclusion, S. reflexum and S. rupestre 'Blue Spruce' are recommended for planting in the central region of Korea during late fall and early winter, as they were more cold tolerant and showed more vigorous regrowth than the other tested plant species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.14
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• pp.97-109
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• 1973

1. The relatively lower prehardening temperature was more effective on increase of the hardiness of the intermediately hardy varieties than the higher one but either the extremely hardy or nonhardy varieties did not respond to the temperature as much as the intermediate types. 2. Five degree Centigrade was generally more favorable than $2^{\circ}C$ on hardening of the plants, especially when frozen at higher temperature for shorter duration. 3. It appears that photoperiod during prehardening and hardening did not play so important role as temperature on the hardiness. 4. The higher the soil moisture content, the higher the frost injury occurred. 5. Application of nitrogen increased markedly the hardiness and % DM of the plants. Percentage of dry matter of young seedlings might be used as an easy and rough criterion for evaluating hardiness since there was a highly significant regression of varietal frost injury on the %DM. 6. Four days appeared to be enough for hardening of plants although the plants increased gradually the hardiness as duration of hardening extended. Dehardening of the plants at relativity higher temperature took place rapidly within one to four days. 7. Under this controlled environment, freezing at about $-8^{\circ}C$ for 24 hours seemed the best for the purpose of evaluating the hardiness to low temperature. 8. It is believed that assessment of frost injury should be done at least one week after freezing. Some varieties showed strong ability to recover from the damage as recovery period was extended. 9. As a whole, Cd 80 and 83 were the most hardy and followed by Cappelle and Maris Otter. Four. winter oats varieties and Jufy I belonged to the intermediate type while the other three spring varieties were nonhardy at all. Peniarth was comparable with Maris Otter in hardiness. S 147 appeared the least hardy among the winter oats varieties. 10. It is evident that water-soluble carbohydrate content is associated with the hardiness to some extent but not primary factor involved in hardiness.

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.13 no.1 s.106
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• pp.58-64
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• 1992

• Proceedings of the Korean Society of Crop Science Conference
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• 1994.10a
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• pp.24-25
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• 1994

• Horticultural Science & Technology
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• v.32 no.2
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• pp.241-251
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• 2014

This study was carried out to develop a simple, rapid and reliable assessment model to predict cold tolerance in Pittosporum tobira, a broad-leaved evergreen commonly used in the southern region of South Korea, which can minimize the possible experimental errors appeared in a electrolyte leakage test for cold tolerance assessment. The modeling procedure comprised of regrowth test and a electrolyte leakage test on the plants exposed to low temperature treatments. The lethal temperatures estimated from the methodological combinations of a electrolyte leakage test including tissue sampling, temperature treatment for potential electrical conductivity, and statistical analysis were compared to the results of the regrowth test. The highest temperature showing the survival rate lower than 50% obtained from the regrowth test was $-10^{\circ}C$ and the lethal was $-10^{\circ}C{\sim}-5^{\circ}C$. Based on the results of the regrowth test, several methodological combinations of electrolyte leakage tests were evaluated and the electrolyte leakage lethal temperatures estimated using leaf sample tissue and freeze-killing method were closest to the regrowth lethal temperature. Evaluating statistical analysis models, linear interpolation had a higher tendency to overestimate the cold tolerance than non-linear regression. Consequently, the optimal model for cold tolerance assessment of P. tobira is composed of evaluating electrolyte leakage from leaf sample tissue applying freeze-killing method for potential electrical conductivity and predicting lethal temperature through non-linear regression analysis.

• Journal of the Korean Institute of Landscape Architecture
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• v.40 no.4
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• pp.127-134
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• 2012

This study was carried out to suggest an experimental base in selecting the cold tolerance of plants. The cold tolerance of the plants were subject to laboratory low temperature treatments and cold processing time were evaluated using both electrolyte leakage and regrowth test. The Logistic model of nonlinear regression analysis was used to evaluate the lethal temperatures that were predicted with the range of $-16.1{\sim}-24.4^{\circ}C$. The order of low-temperature resistance was Sedum reflexum > S. spurium > Ophiopogon japonicus > S. album > S. takevimense > Dianthus chinensis. At the lowest temperature of $13.4^{\circ}C$ the electrolyte leakage value of the plants were lower than 50% demonstrating that they could be applied stably to the roof installed in Korea during the winter with the lowest temperature of $-13.5^{\circ}C$.