• Development and Reproduction
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• v.4 no.1
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• pp.125-131
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• 2000

This study has used differential display-PCR, to amplify genes transcribed during the ovarian maturation induced by human chorionic gonadotropin (hCG). The cDNA expressed at the times of acquisition of oocyte maturational competence in red seabream (Pagrus major) following treatment with hCG was amplified and cloned. A full-length of cDNA for p. major was isolated using differential display-PCR and 5'RACE. This cDNA clone contained 2,662 nucleotides including the open reading frame that encoded 434 amino acids. Homology analyses, using the GenBank and EMBL general database searches, indicated that the nucleotides sequence of the cDNA does not have high homology with any other genes. This cDNA was judged to be a gene, which induction of maturational competence coincides with increase of mRNA related ovarian maturation. Consensus sequences which were consistent with protein kinase C phosphorylation sites and casein kinase II phosphorylation sites were identified. in vitro, the transcription level of mRNA related ovarian maturation increased between 9hr and 24hr following treatment of ovarian follicles with hCG. It was also increased after GtH-II (300 ng/ml) stimulation. Furthermore, in vivo, mRNA related ovarian maturation was rarely expressed prior to the acquisition of oocyte maturational competence, but was strongly expressed after the acquisition of oocyte maturational competence, suggesting that the hCG induction of maturational competence is brought about by the de novo synthesis of the mRNA related ovarian maturation in p. major.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.3
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• pp.299-305
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• 1994

Egg distribution and mortality rate for anchovy, Engraulis japonica, and sardine, Sardinops melanosticta, during spring in the southern waters of Korea were estimated using the data from ichthyoplankton surveys conducted from March to April 1991, 1992. Anchovy and sardine eggs were found simultaneously in high density in the southwestern waters of Tsushima Island during March 1991 and late in March${\sim}$early in April 1992. Distribution areas of anchovy and sardine eggs in 1992 was extended to the middle area of the southern waters compared to 1991. Sea surface temperatures in the survey area ranged from 8.6 to $17.8^{\circ}C$. Anchovy and sardine eggs occurred with high densities between $14^{\circ}$ and $15^{\circ}C$. Instantaneous mortality coefficients were estimated to be 0.268/day for anchovy, and 0.132/day for sardine during $4{\sim}15$ March in 1991.

• The Korean Journal of Zoology
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• v.26 no.3
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• pp.193-202
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• 1983

Inversion at variable time of the uncleaved eggs of Rana nigromaculata and Rana dybowskii was emplyed to study the mechanisms of early embryogenesis. The response to inversion varied between those two spcies. If the eggs were inverted at early time it was possible to change the site of the first cleavage furrow of Rana nigromaculata-it appeared on the original vegetal hemisphere (OpG side). However, the first cleavage furrow of Rana dybowskii was not changed at all no matter what its inversin time was-it always appeared on the original animal hemisphere (G. side). In the inverted Rana nigromaculata embryos the size of the blastomeres on the vegetal hemisphere was always smaller than those of animal hemisphere. On the ocntrary, the cleavage pattern of Rana dybowskii was not altered. Results of the histological works for the inverted eggs suggest that the different responses to inversion might be caused by the fact that the extent to which the redistribution of the egg components such as cleavage nuclei and yolk platelets are different.

• Development and Reproduction
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• v.10 no.4
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• pp.263-269
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• 2006

To evaluate the potentiality of industrial use of cysteine proteinase inhibitor (cystatin) of tilapia egg and serum stability of the tilapia cystatin on low temperature storage and heat treatment was studied. When the eggs were stored at $4^{\circ}C$ for 3 days the cystatin activity was not changed much, while the supernatant of egg homogenate lost its cystatin activity significantly, remaining only about 65% of initial activity. When the eggs and serum were subjected to repeated freeze at $-20^{\circ}C$ and thaw at room temperature once a day, the egg cystatin was decreased after 5 cycles of freeze and thaw. However the serum cystatin was not changed by the 5 times repetition of freeze and thaw. More than 80% of egg cystatin activity was remained when the egg was heated at $35^{\circ}C$ for 30 min, but less than 10% was remained when heated at $50^{\circ}C$. On the other hand, the serum cystatin was very resistant to heat, remaining about 74% after heating at as high as $80^{\circ}C$ for 30 min. In summary, the egg cystatin was more stable when stored as intact form of egg rather than as supernatant of homogenate when stored at refrigeration. Egg cystatin was relatively stable against repeated freeze-thaw, and serum was found to be more stable in cysteine proteinase inhibitory activity than egg. Egg cystatin was not very resistant to heat treatment, while serum cystatin was quite resistant to high temperature heat treatment. These results suggest that tilapia egg and serum, especially the serum, would be a useful source for cysteine proteinase inhibitor in surimi production.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.2
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• pp.220.2-220.2
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• 2012

대기 중 수증기의 불규칙한 분포와 운동은 대기의 굴절율을 시간에 따라 불규칙하게 변화시킨다. 전파 관측 중 대기의 굴절율이 짧은 시간 동안에 크게 변하면 천체가 안테나 주빔의 중앙에서 벗어나는 것처럼 관측되고, 이는 마치 안테나 지향 오차가 커지는 것과 같은 효과를 준다. 이러한 현상을 대기 난굴절(Anomalous Refraction)이라고 한다. KVN 안테나 성능 시험 관측 중 심할 경우 수십초의 시간 규모에서 수십 각초 이상 안테나 지향 오차가 커지는 현상이 관측되어 각 사이트의 대기 난굴절 특성에 대하여 모니터링을 시행하였다. 22GHz와 43GHz에서 KVN 사이트의 대기 난굴절 특성을 파악하기 위하여 2010년 4월부터 2011년 4월까지 각 계절마다 세 사이트에서 시험 관측을 진행하였다. 관측 결과로부터 계절별, 낮과 밤등을 기준삼아 KVN 사이트의 대기 난굴절의 특성을 소개한다.

• The Korean Journal of Mycology
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• v.39 no.1
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• pp.7-10
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• 2011

Orchid mycorrhizal fungi (OMF) were examined in roots of the six terrestrial species of orchids collected in Ulleung Islands. Seven OMF isolates from the roots of orchids were identified based on morphological and molecular characters. Internal transcribed spacer region of OMF DNA was amplified using basidiomycete-specific ITS primers, ITS1-OF and ITS4-OF. OMF beloning to Tulasnellaceae and Ceratobasidaceae was identified through molecular analysis.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.266-271
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• 2012

This study was carried out to evaluate the land suitability for southern-type garlic cultivation associated with both temperature and soil constraints. The suitability analysis was conducted with hourly temperature data from 2001 to 2010 at all fifty seven meteorological stations and the soil-based suitability map of garlic provided by Rural Development Administration. Firstly the temperature data were processed by the growth stages (germinating, bulbing, and winter vegetation season), and then were adopted to limit the irrelevant lands. Next, as a result of overlaying each soil and temperature suitability map, the total 274,339 ha of area was mapped as highly suitable or suitable for southern-type garlic cultivation and the top four of the largest classified si-guns were identified as Naju, Jeongeup, Gochang, and Jinju. On the other hand, the statistical records of KOSIS (KOrea Statistical Information Service) showed lower amount of cultivation area than the analyzed results in the major production sites, Goheung, Sinan, Haenam, and Muan. However, it should not be regarded as exceptional because farmer's preference might not correspond to potential land usability.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.5
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• pp.21-27
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• 2003

Five warm-season turfgrass [Zoysia japonica Steud., Stenotaphrum secundatum (Walt.) Kuntze, Buchloe dactyloides (Nutt.) Engelm., Eremochloa ophiuroides (Munro.) Hack. and Cynodon dactylon (L.) Pers.] were overseeded with 2 cool-season turfgrasses [Poa pratensis L. and Festuca arundinacea Schreb.] to examine suitability of these species for planting in mixture for producing a year-round turf. Percent of warm-season and cool-season turfgrasses in the mixture were estimated for 5 years. Also, to evaluate the fertilization times of the warm-season and cool-season turrfgras mixtures. Eremochloa ophiuroides and Buchloe dactyloides were not suitable for warm-season and cool-season turfgrass mixtures. However, Zoysia japonica, Stenotaphrum secundatum and Cynodon dactylon were suitable for warm-season and cool-season turfgrass mixtures. Zoysia japonica, Stenotaphrum secundatum and Cynodon dactylon were showed approximately 50% botanical composition five years after cool-season turfgrass overseeding. And, those three warm-season turfgrass mixtures showed approximately 20% visual ratings in winter period. Fertilization in early spring and late autumn had made cool-season turfgrass dominated in mixtures. Therefore, fertilization times in warm-season and cool-season turfgrass mixtures was desirable in summer period.

• Journal of Digital Convergence
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• v.13 no.4
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• pp.377-385
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• 2015

The purpose of the study was to perform trend analysis of the exercise content i.e. element difficulty distribution scores, element group distribution scores obtained by the athletes during 2014 Korea Cup International Gymnastic finals. The conclusion drawn from the analysis are as follows: Firstly in terms of tournament technical difficulty value, Korean athletes showed characteristics of reliance on swing element rather than strength hold element which could actually secure higher difficulty scores. Secondly, skill acquisition of higher difficulty value is demanded by taking advantage of familiar characteristics from the swing element i.e. Jonasson and Roll bwd. Slowly with str. arms and body to swallow (2s.). Thirdly, development of sensory training and strengthening exercise program are essential to enhance strength hold technique.

• Korean Journal of Ecology and Environment
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• v.53 no.2
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• pp.195-207
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• 2020

In Korea, specific thermal elements such as annual mean temperature (AMT) 13℃, 14℃, and Kira's coldness index (CI) -10℃, have been suggested about the northernmost distribution of the warm-temperate evergreen broad-leaved forest zone. We reviewed the relationship between three thermal elements and the actual distribution of evergreen broad-leaved woody plants or its communities. Thiessen and Kriging method using point-data calibrated by seasonal lapse rate according to altitude were utilized for the spatial distribution pattern analysis. Several phytoclimatic maps were also produced in order to compare different thermal values. We identified that the AMT 13℃ was the best thermal element to demarcate the northern limit of the warm-temperate forest zone. Its area was estimated ca. 20,334 ㎢ and larger than those of other thermal elements. We concluded that an indirectly fabricated index i.e. CI -10℃ is useless and it was enough for a direct value of AMT 13℃ to represent the northern-limit distribution of warm-temperate forest zone, at least in Korea. Further researches on the reciprocity between floristic regions and phytoclimate zones are raised.