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365 nm LED laser treatment on beagle for gingival whitening without gum dermabrasion (잇몸 박피술 없는 치은 미백을 위한 비글에서 365 nm LED 레이저 치료)

  • Lee, Sung-Ho;Kim, Ryun Kyung;Seo, Na-Ri;Lim, Ho-Kyung;Byun, Soo-Hwan;Lim, Young-Joon;Kim, Soung-Min;Lee, Jong-Ho
    • Journal of Dental Rehabilitation and Applied Science
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    • v.32 no.2
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    • pp.117-122
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    • 2016
  • Purpose: Gingival whitening is one of dental treatment purposes which is close to treating aesthetic disorders. Initial gingival whitening treatment was done by dermabrasion using a high power Diode Laser. However, this treatment method cannot be free from any infection or pain after the treatment. Therefore, we have decided to progress gingival whitening treatment using a low power LED laser. Materials and Methods: The laser was irradiated on pork meat then the safety of output power, temperature change and skin denaturalization was measured. Bison 365 nm LED laser was irradiated on oral mucosal pigment of a 15 - 20 kg beagle for 15 min for 1 - 2 weeks, one or two times each. Any pigment loss was checked through Hematoxyline-Eosin staining. Results: The melanin pigments at the area of 365 nm LED Laser irradiation were decreased. Conclusion: The 365 nm LED Laser proposed in this study is considered to compensate the bleaching effect achieved by either using Diode laser or surgical methods.

Eleutherosides Extraction from Acanthopanax sessiliflorus Seeman and Eleutherococcus senticosus Maxim Using an Enzymatic Process (효소적 생물전환을 이용한 토종오가피와 가시오가피의 Eleutheroside B, E의 추출 함량 최적화)

  • Kim, Na-Ri;Park, Jong-Soon;Lee, Deuk-Sik;Shim, Jae-Hoon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.9
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    • pp.1273-1278
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    • 2016
  • In this study, we optimized conditions for extraction of eleutherosides B and E from stem powder of Acanthopanax. To enhance eleutheroside B and E yields, Acanthopanax sessiliflorus Seeman and Eleutherococcus senticosus Maxim were pre-incubated with the following enzymes: Celluclast, Viscozyme L, Lactozyme, Lecitase, and Novozyme 33095. Treatment with Novozyme 33095, a commercial pectinase, for 3 h resulted in the highest yields of eleutherosides B and E from A. sessiliflorus and E. senticosus. Compared with extraction at $121^{\circ}C$ for 120 min, at $121^{\circ}C$ for 15 min after Novozyme 33095 pre-incubation increased eleutheroside B and E yields from A. sessiliflorus by 7% and 17%, respectively. In the case of E. senticosus, eleutheroside B and E yields increased by 25% and 29%, respectively.

Vegetation Structure and Dynamics of a Betula davurioa Forest in Mt. Chiri (지리산(智異山) 물박달나무림(林)의 식생구조(植生構造)와 동태(動態))

  • Ahn, Hyun-Chul;Lee, Jeong-Hwan
    • Journal of Korean Society of Forest Science
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    • v.87 no.3
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    • pp.445-458
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    • 1998
  • The present study focused on the vegetation structure and dynamics of Betula davurica forest were to provide the information for management, distribution and utilization of B. davurica forest in Mt. Chiri national park, southern Korea. The layer structure of B. davurica forest were consist of tree layer(18 species), middle layer(29 species), shrub layer(43 species) and herb layer(68 species). According to the importance value of stratification of layers, In tree layer, B. davurica dominant the highest value of 123.97%, Alnus hirsuta 49.04., Castanea crenata 25.58%, Quercus serrata 25.02%, Stewartia koreana 14.49%, in middle layer were B. davurica 70.81%, Q. serrata 37.74%, Fraxinus rhynchophylla 25.35% Lindera erythrocarpa 24.52%, S. koreana 18.86%, shrub layer were Sasa borealis 28.28%, L. erythrocarpa 24.94%, Rhus trichocarpa, 20.42%, Stephanandra incisa 19.90% and herb layer Schizandra chinensis 18.51%, Aster scaber 12.26%. Disporum smilacinum 10.63% etc, respectively. Results of chi-square test statistics based on presence-absence parameters, the positively associated species pair were Quercus variablis - Q. acutissima, Stewartia koreana - Symplocos paniculata, Acer pseudo-sieboldianum - Quercus mongolica. And covariation was calculated based on quantitative measure of density. The results showed that the association and covariation values among species not agreed with each other. The frequency distribution of DBH Betula davurica species showed reverse J-shaped, therefore it's seems to retrain as a dominant species. When predicting succession in Betula davurica forest, it is seedling and saplings were not richness, succession was governed by competition of hardwood species of subtree and tree layer.

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Future Projections of Köppen Climate Shifts in the Asia Regions Using A2 Scenario (A2 시나리오를 이용한 아시아 지역 기후대의 변화 전망)

  • Shin, Sang Hoon;Bae, Deg Hyo
    • Journal of Korea Water Resources Association
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    • v.46 no.3
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    • pp.253-265
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    • 2013
  • The objective of this study is to analyse the current climate zone applied by K$\ddot{o}$ppen climate classification and the future climate zone projected by the A2 scenario in Asia regions. The spatial and temporal variations of precipitation and temperature were also analyzed. As regards to the result of analysis on the variation of climate factor, temperature and precipitation will be increasing $4.0^{\circ}C$ and 12% respectively in the 2080s comparing with the reference period (1991~2010). Spatially, the range of temperature increase on the high latitude area is higher than that on the low latitude area. The precipitation will be increasing averagely in the overall area, but the spatial unequal distribution of precipitation will be intensified. At the result of the future climate zone, the area of warm climates will be increasing while the area of cold climates will be decreasing. In 2080s, the temperature will be increasing as much as 7.2% and 1.9% on the Tropical climates and Arid climates respectively, but it will be decreasing as -2.4%, -4.9% and -1.8% on the Warm temperate climates, Cold climates and Polar climates respectively. Furthermore, the part of Savannah climates and Desert climates will be mostly increasing. It is mainly caused by the temperature increase and desertification impact according to global warming.

Antioxidant Capacity of Colored Barley Extracts by Varieties (계통별 유색보리의 항산화효과)

  • Song, Eun-Seung;Park, Soo-Jin;Woo, Na-Ri-A;Won, Mi-Hee;Cho, Jae-Seong;Kim, Jeong-Geon;Kang, Myung-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.10
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    • pp.1491-1497
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    • 2005
  • This study evaluates the antioxidant capacity of 40 varieties of colored barley and hulled barley. For investigation of the antioxidant capacity as well as the contents of anthocyanin, color intensity, total phenolic acids contents, SOD-like activity, samples were extracted in ethanol with 0.1$\%$ TFA. The content of anthocyanin was measured spectrophotometrically. The total phenolic acid content was analyzed by using th Folin-Ciocalteu method, and the color intensity was determined by using the color difference meter. The results showed that in the anthocyanin content and color intensity of colored barley there were significant differences according to their varieties. The resulting concentration levels were divided into three groups: Group I (high) Group II (medium), and Group III (low). In the measurements of antioxidant capacity significant differences(P < 0.05) were found among varieties in those groups. Colored barley was higher in electrondonating capability, SOD-like activity, iuhibitory effects on lecithin oxidation, and hydrogen radical- scavenging ability Meanwhile, from different antioxidant tests, No. 26, 27, 34, and 35 varieties measured high in antioxidation ability fell into Group I high in the anthocyanin content. This results suggest that the anthocyanin content has a significant relationship on antioxidant activity.

A Survey on the Sensory Preference for Making Summer Kimchi by Nationwide Region (여름철 배추김치 담금시 지역별 관능적 선호도 조사)

  • Cha, Yong-Jun;Kim, Hun;Cho, Wo-Jin;Jung, Yeon-Jung;Lee, Young-Mi;Kim, Eun-Jeong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.3
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    • pp.393-399
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    • 2003
  • The purpose of this study was to investigate the preferred methods for making summer kimchi as a basic research of making commercial kimchi. Questionnaire were collected from 590 housewives nationwide in Korea, and the data were analyzed by SPSS program. The results were as follows: (1) The average salting time of Chinese cabbage was 3~5 hrs when the combined method of dry and brine salting was used, regardless of the region. (2) Seven jeotkals (salt-fermented fishes) including anchovy, anchovy juice, shrimp, northern sand lance juice, hair-tail viscera, flatfish and yellow corvenia were mainly used in kimchi. Among them, anchovy and anchovy juice jeotkals were preferred to all others in Southern area (Busan, Gwangju, Gyeongnam, Gyeongbuk, Jeonnam), while shrimp jeotkal in Seoul, Gwangju, Jeonbuk, Chungnam and Jeju, and northern sand lance juice jeotkal in Daegu, Daejeon and Gyeonggi, respectively. In most regions, however, blending type of 2 jeotkals was used in kimchi. (3) Eleven ingredients such as red pepper, garlic, ginger, green onion, radish, leek, onion, carrot, sugar, sesame and MSG were used as basic components for making kimchi. In particular, MSG was used as a basic ingredient regardless of region and age. However, a standard taste for making kimchi was depended on housewife in this study.

The Analysis of the Plant Distributional Pattern in Yugu Stream (Gongju, Chungnam) (유구천(충남, 공주)의 식물 분포 현황분석)

  • Moon, Ae-Ra;Lee, Youl-Kyong;Park, Jeong-Mi;Jang, Chang-Gee
    • Korean Journal of Environmental Biology
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    • v.30 no.2
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    • pp.107-120
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    • 2012
  • This study was carried out to illiminate the diversity of vascular plants by making list of rheophyte and hydorphyte plants at Yugu stream. This study was conducted from Aug., 2009 to Sept. 2010 at 7 points. Based on the voucher specimens, vascular plants from investigated areas were 88 families 229 genera 290 species 3 subspecies 30 varieties 5 forms, totally 328 taxa. Korean endemic plants were 3 species such as Forsythia koreana (Rehder) Nakai (planted) and Paulownia coreana Uyeki (planted), and Weigela subsessilis (Nakai) L.H. Bailey. The rare and endangered plants were designated by Korea Forest Service were 4 taxa, such as Penthorum chinense Pursh, Acorus calamus L., Lilium callosum Siebold & Zucc. and Iris ensata var. spontanea (Makino) Nakai. Floristic special plants were totally 26 taxa, which were grade V of 2 taxa, grade IV of 2 taxa and grade III of 9 taxa. Naturalized plants were 27 taxa, that was 8.3% of whole discovered vascular plants in this area. Even naturalized plants has not influence on the vegetation of Yugu stream. Since village was composed along the stream and the roads and banks were linked, naturalized plants were flowed continuously into this region. So, strategy of the conservation for this region should be designed. Based on flora, 3 types of group were discriminated such as main stream type (4 points), branch stream type (2 points) and reservior type (1 point). Each group has difference in composition of plants.

Metabolic Engineering of the Brassinosteroid Biosynthetic Pathways (Brassinosteroid의 대사공학)

  • Lee, Mi-Ock;Song, Ki-Hong;Lee, Hyun-Kyung;Jung, Ji-Yoon;Choe, Vit-Nary;Choe, Sung-Hwa
    • Journal of Plant Biotechnology
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    • v.29 no.2
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    • pp.139-144
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    • 2002
  • Sterols play two major roles in plants: a bulk component in biological membranes and precursors of plant steroid hormones. Physiological effects of plant steroids, brassinosteroids (BRs), include cell elongation, cell division, stress tolerance, and senescence acceleration. Arabidopsis mutants that carry genetic defects in BR biosynthesis or its signaling display characteristic phenotypes, such as short robust inflorescences, dark-green round leaves, and sterility. Currently there are more than 100 dwarf mutants representing 7 genetic loci in Arabidopsis. Mutants of 6 loci, dwf1/dim1/cbb1, cpd/dwf3, dwf4, dwf5, det2/dwf6, dwf7 are rescued by exogenous application of BRs, whereas bri1/dwf2 shares phenotypes with the above 6 loci but are resistant to BRs. These suggest that the 6 loci are defective in BR biosynthesis, and the one locus is in BR signaling. Biochemical analyses, such as intermediate feeding tests, examining the levels of endogenous BR, and molecular cloning of the genes revealed that dwf7, dwf5, and dwf1 are defective in the three consecutive steps of sterol biosynthesis, from episterol to campesterol via 5-dehydroepisterol. Similarly, det2/dwf6, dwf4, and cpd /dwf3 were shown to be blocked in D$^4$reduction, 22a-hydroxylation, and 23 a-hydroxylation, respectively. A signaling mutant bril/dwf2 carries mutations in a Leucine-rich repeat receptor kinase. Interestingly, the bri1 mutant was shown to accumulate significant amount of BRs, suggesting that signaling and biosynthesis are dynamically coupled in Arabidopsis. Thus it is likely that transgenic plants over-expressing the rate-limiting step enzyme DWF4 as well as blocking its use by BRIl could dramatically increase the biosynthetic yield of BRs. When applied industrially, BRs will boost new sector of plant biotechnology because of its potential use as a precursor of human steroid hormones, a novel lead compound for cholesterol-lowering effects, and a various application in plant protection.

Study on the Nutritional Composition and Antioxidative Capacity of Mulberry Fruit(Ficus-4x) (4배성 휘커스(Ficus-4x)오디품종의 영양성분 및 항산화능 검색)

  • Kim, Ae-Jung;Kim, Mi-Won;Woo, Na-Ri-Yah;Kim, Sun-Yeou;Kim, Hyun-Bok;Lim, Young-Hee;Kim, Myung-Hee
    • Korean Journal of Food Science and Technology
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    • v.36 no.6
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    • pp.995-1000
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    • 2004
  • Nutritional composition and antioxidative capacity of mulberry fruit (Ficus-4x) were investigated for evaluation as new red-colored fruit. Contents of moisture, crude fat, crude protein, and vitamin C were similar, whereas that of crude ash was higher, to those of other berry fruits. Contents of minerals (Ca, 14.33 mg/100 g; P, 39.98 mg/100 g; Fe, 6.01 mg/100 g; Zn, 4.04 mg/100 g; Mn, 2.26 mg/100 g), particularly Fe, were higher than those of other berry fruits. Hardness, springness, cohesiveness, gumminess, and chewiness of mulberry fruit were higher, and color values (L, 36.03; a, 1.80; b, 1.54) were lower than those of strawberry. Relative scavenging activities of mulberry fruit methanol extract and its cyanidine 3-glucoside on 1, 1-diphenyl-2-picrylhydrazy radical (DPPH) were 35.7 and 78.2%, respectively, using butylated hydroxyanisole (BHA) as standard. Antioxidant activities in corn oil (peroxide values and conjugated dienoic acid) were tertiary butylhydroquinone (TBHQ) > mulberry fruit ethanol extract > mulberry fruit water extract > butylated hydroxytoluene (BHT) > tocopherol. Results show mulberry fruit can be very useful red-colored fruit for development of functional foods with beneficial effects on radical scavenging and antioxidative capacities.

Overcoming F1 Sterility of Intersectional OA Lily Hybrids by Somatic Chromosome Doubling and Production of Subsequent Progeny (체세포 염색체 배가법을 이용한 백합 OA 종간 잡종의 불임극복과 후대획득)

  • Chung, Mi-Young;Chung, Jae-Dong;Jee, Sun-Ok;Tuyl J. M. Van;Lim, Ki-Byung
    • Journal of Plant Biotechnology
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    • v.31 no.2
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    • pp.103-108
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    • 2004
  • This study was carried out to develop the efficient methods for the subsequent progeny of intersectional hybrid between Oriental and Asiatic lily hybrids. The pollen fertility and germination ability of 3 different allotetraploids (OAOA) after somatic chromosome doubling was ranged from 0 to 80 percent on artificial pollen germination medium. The number of BC$_1$ progeny using allotetraploid of F$_1$ OA-hybrid as male and female parent was different. The efficiency of BC$_1$ progeny production was increased when F$_1$OA-hybrids was used as male rather than as female parent. And in back crosses of F$_1$ OA-hybrids with the Asiatic and Oriental hybrids, Asiatic hybrids showed higher efficiency on BC$_1$ progeny production. The ploidy level between 2x or 4x Asiatic hybrid and allotetraploid F$_1$ OA-hybrid was determined and showed higher progeny production in 2x-4x crosses rather than 4x-4x.