• Journal of Mushroom
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• v.3 no.3
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• pp.109-114
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• 2005

Attempts were made to control Diatrype stigma occurred on the bed-log of shiitake by resistant shiitake strains. In selection test of resistant shiitake strains, 67 out of 77 strains tested were proved to be resistant to D. stigma. Among them, 13 strains including KFRI 5 were effective to inhibit the access of D. stigma, and 7 strains including KFRI 180 remarkably invaded the territory of D. stigma. Among 31 shiitake strains made by hybridization of resistant strains for D. stigma, 8 strains including KFRI 537 inhibited the access of D. stigma, and 4 strains including KFRI 545 invaded the territory of D. stigma. The effects of temperatures and inoculation orders to the resistance were confirmed in PDA plates and test tubes filled with sawdust of Quercus acutissima. Four kinds of temperature treatments as follows were tested: (1) continuous incubation at $14^{\circ}C$, (2) continuous incubation at $25^{\circ}C$, (3) changing of incubation temperature from $14^{\circ}C$ to $25^{\circ}C$ as soon as mycelia of both shiitake and D. stigma meet together, (4) changing of incubation temperature from $25^{\circ}C$ to $14^{\circ}C$ as soon as mycelia of both shiitake and D. stigma meet together. Three kinds of inoculation procedure were tested: (1) inoculation of shiitake 3 days ahead of D. stigma inoculation, (2) inoculation of D. stigma 3 days ahead of shiitake inoculation, (3) simultaneous inoculation of both fungi. In PDA plate test, the strain KFRI 137 showed outstanding ability to inhibit mycelial growth of D. stigma and the strain KFRI 180 invaded into the territory of D. stigma in most of treatments. Hybrid strains, KFRI 545, 546, and 547 were more resistant than their parent strains, KFRI 488 and 405. In test tube examinations, all the strains of shiitake showed high resistance at the treatment of change in temperature from $14^{\circ}C$ to $25^{\circ}C$ when mycelia of both shiitake and D. stigma meet together. On the other hand, resistance of all the strains growing at $25^{\circ}C$ decreased when the temperature was changed into $14^{\circ}C$ after mycelia of both fungi. In these cases, the resistance reached to 7~20% of the highest resistance. The strain KFRI 259 invaded the territory of D. stigma, contrary to PDA plate test. Among the strains, KFRI 393 strain was the most resistant under the continuous incubation at $25^{\circ}C$.

• Korean Journal of Plant Resources
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• v.30 no.2
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• pp.160-166
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• 2017

This study was performed to develop the efficient phytoremediation model in the paddy soil contaminated with heavy metals by cultivating Pteris multifida and Artemisia princeps with different mixing ratios (1:0, 8:1, 6:1, 4:1). As a result of investigating the heavy metal accumulation of each plant per dried material (1 kg), content of arsenic and cadmium was the highest in aerial part of P. multifida (169.82, $1.70mg{\cdot}kg^{-1}DW$, each) among the treated group. Lead content was the highest ($12.58mg{\cdot}kg^{-1}DW$) in the aerial part of P. multifida cultivated with 8:1 mixed planting. But the content of copper and zinc was the highest (33.94, $61.78mg{\cdot}kg^{-1}DW$, each) in the aerial part of A. princeps with 8:1 treatment. Regardless of heavy metals, plant uptake from the $1m^2$ soil was the highest in 4:1 mixed planting group, which showed the best yield of A. princeps.

• Applied Microscopy
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• v.39 no.2
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• pp.107-115
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• 2009

The programed cell death of the cutaneous epithelial tissue during tail regression stages in anuran tadpoles of the blackspotted frog, Rana nigromaculata were visualized by the histochemical and transmission electron microscopic techniques. Metamorphotic changes in the tail regression during the period of the Shumway stage number 31 to 33 are characterized by the disappearance of mucous layer and formation of compound epithelium through cutaneous thickening. Following the TUNEL (terminal deoxynucleotidyl transferase-mediated biotinylated d-uridine triphosphate nick end labeling) staining technique, the apoptotic cells were detected at the distal region of the tail skin initially, but they can be seen at the proximal region according to their following development. It has been also revealed that the number of the TUNEL-positive cells gradually increased from apical to basal direction of the epithelial layers during the tail regressing stages. Following the TEM observation, the early apoptotic cells shown in the epithelium demonstrated condensation and margination of the chromatin material at the nuclear periphery. Another epithelial apoptotic cells were shown nuclear fragmentation, membrane blebbing and cytoplasmic condensation. Following the process of the apoptotic degradation, well preserved organelles and nuclear fragments can be identified in the cytoplasm of lysosome-rich cells, however they soon reduced to lysosomal residual bodies through the progressive degradation.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.2 no.1 s.2
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• pp.41-52
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• 2003

The purpose of this study was to test the effects of the pedestrian green signal adjustment on clearance of the turning vehicles impeding the through traffic flow at the signalized intersections, and thereby, suggest some operational criteria for adjustment of the pedestrian green signal. In order to test such effects, the pedestrian green time was adjusted so that it could started a few seconds later than the vehicle green time during peak hours, and thereby, the turning vehicle volume not cleared at the intersection was measured by extending the time gap by 2 seconds. (In general, the pedestrian green signal turns on at the same time as the vehicle green signal.) The results of this test can be summed up as follows; first, the longer the time gap was, the turning vehicle volume not cleared from the intersection decreased more. Second, in case there existed a storage space between intersection and crosswalk the effect of the turning vehicles on the through traffic flows was minimal. Third, at the pelican, the effect of the turning vehicles on the through traffic flow was minimal due to the structure of the intersection and the phase sequence. In conclusion, it was found that the adjustment of pedestrian green signal had the effect of enhancing the intersection operation. When adjusting the pedestrian green signal, it was deemed necessary to thoroughly survey the geometric structure of the intersection and collect the data on the turning traffic volume and thereby, apply the results of analysis flexibly to each intersection.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.2
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• pp.163-168
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• 2015

In order to perform estimations with high reliability, it is necessary to deal with the tail part of the cumulative distribution function (CDF) in greater detail compared to an overall CDF. The use of a generalized Pareto distribution (GPD) to model the tail part of a CDF is receiving more research attention with the goal of performing estimations with high reliability. Current studies on GPDs focus on ways to determine the appropriate number of sample points and their parameters. However, even if a proper estimation is made, it can be inaccurate as a result of an incorrect threshold value. Therefore, in this paper, a GPD based on the Akaike information criterion (AIC) is proposed to improve the accuracy of the tail model. The proposed method determines an accurate threshold value using the AIC with the overall samples before estimating the GPD over the threshold. To validate the accuracy of the method, its reliability is compared with that obtained using a general GPD model with an empirical CDF.

• Korean Journal of Ecology and Environment
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• v.43 no.1
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• pp.142-149
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• 2010

Tadpole shrimps are aquatic crustaceans adapted to temporary water pool. Since they have been recorded as the living fossil, there are a number of reports on their past and present distribution as well as their morphology and reproduction in foreign countries. However, there are few studies of biology and ecology of tadpole shrimps despite registration as an endangered species by Ministry of Environment, in Korea. This study was carried out to examine the distribution and habitat characteristics of tadpole shrimps (Triops longicaudatus) collected from May 2006 to July 2008 in Korea. Tadpole shrimps were found from the paddy fields and ephemeral pools at 21 countries in South Korea. They appeared between May and July and survived for 30 days, and temperatrue range was $10.7^{\circ}C$ to $33.5^{\circ}C$. The average turbidity of experimental paddy fields was 176.8 (${\pm}239.3$) NTU, and controlled field was 28.9 (${\pm}26.7$) NTU. The life pattern of tadpole shrimps is adapted to a temporary water pool and unstable environmental conditions, and also there was great effect of agricultural activity on the development and growth of tadpole shrimps.

• Korean Journal of Plant Taxonomy
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• v.45 no.4
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• pp.362-368
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• 2015

Two new and one unrecorded hybrids of the genus Asplenium were newly found in Korea. They were proved to be hybrid based on intermediate morphology between putative parents. The first hybrid is $A.{\times}uiryeongse$ C.S. Lee & K. Lee (Aspleniaceae), nom. nov. (vernacular name: geo-mi-sa-cheol-go-sa-ri). This new hydrid is a cross between the Asian walking fern, A. ruprechtii and A. pekinens in Uiryeong-gil, Mt. Bukansan, Seoul, Korea. The other new one occurs in Mt. Seongsan in Yeoncheon-gun, Gyeonggi-do, Korea, $A.{\times}montanus$ C.S. Lee & K. Lee, nom. nov. (vernacular name: san-kko-ri-go-sa-ri) which might have experienced multiple hybridization events between A. ruprechtii ${\times}$ A. trichomanes and A. incisum following the hybrid between A. ruprechtii and A. trichomanes. In addition, one unrecorded hybrid, $A.{\times}kitazawae$ Kurata & Hutoh (vernacular name: geo-mi-dol-dam-go-sa-ri), reported first from Japan, is found in natural habitats in Do-dong, Daegu-si, and is a hybrid, between the Asian walking fern and A. sarelli. These hybrid taxa grow in sympatric regions with both putative parental species. The diagnostic characters for each taxon and evidence for their hybridization have been suggested. Descriptions, illustrations, and photographs of these hybrid taxa in their habitats are provided from Korea.

• Journal of the Korean Society of Food Science and Nutrition
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• v.13 no.2
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• pp.149-155
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• 1984

Raw and cooked pig's head, feet and tails were evaluated for yield, proximate composition, caloric value, and the contents of amino acids, minerals and vitamins. The yields of the raw pig's head, feet and tails were 67.4%, 46.1% and 67.6%, respectively. Corresponding values for the cooked were 54.1%, 34.3% and 47.6% respectively. No significant differences were observed for proximate composition between these samples and any other source3 of the meats. The high energy values, 253.8-310.5 Cal/100 g, of the samples were largely due to the fat content. Among the essential amino acids, these products showed a higher lysine, leucine and threonine content, and lower methionine and tryptophan content. The total amounts of non-essential amino acids were more than half of those of all amino acids. Neither the raw nor the cooked pig's head, feet and tails contained vitamin A. On these data, pig's head, feet and tails tissue have a relatively low in vitamin B content as compared to conventional pork cuts.

• The Korean Journal of Soil Zoology
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• v.4 no.2
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• pp.101-106
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• 1999

Fibrinolytic enzyme is thought to be involved in extracellular matrix remodeling during regeneration. We investigated the expression and characterization fibrinolytic enzyme activity during earthworm tail regeneration. Electrophoretic analysis of fibrinolytic enzymes induced during regeneration revealed that at least seven types of fibrinolytic enzymes were expressed, which had molecular weight of 12, 19, 23, 27, 32, 45 and 58 kDa, respectively. These fibrinolytic enzyme activities were dramatically increased within 1 day after amputation. These activities were maintained by 7 days postamputation, followed by decrease to control level from 14 days after amputation. Alltypes of fibrinolytic enzyme activities were inhibited by treatment of PMSF and aprotinin, and were insensitive to EDTA and exogenous Ca$^{2+}$. These results indicate that the fibrinolytic enzymes are serineproteinase. Other characteristics including specificities for extracellular matrix proteins are under investigation. Based on these results, we are trying to find out the relationship among expression of proteinases, extracellular matrix remodeling, and dedifferentiation, which are believed to be essential processes during regeneration.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.86-86
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• 2019

현삼과(Scrophulariaceae)에 속하는 긴산꼬리풀(Veronica longifolia L.)은 벽자색의 긴 꽃이 총상화서로 달려 청량감을 자아내어 분화소재로 이용한다면 단조로운 실내의 분위기를 환기할 수 있다. 따라서, 조경 및 관상용 식재에 용이한 긴산꼬리풀의 재배는 2018년 5월 15일에 파종하여 생산된 유묘를 2018년 7월 16일에 정식하여 2018년 10월 15일까지 수행하였다. 기비실험은 코트비료(싱싱코트)를 포트당 0, 10 및 20알 처리하였다. 추비는 hyponex (N-P-K, 4-6-6)를 4주 간격으로 총 3회 각 0, 1000 및 $2000mg{\cdot}L-1$로 엽면시비 하였다. 광량 실험은 무차광, 55 및 75% 차광막을 설치하여 수행하였다. 왜화제 종류 및 농도 실험은 dinizonazole(빈나리)과 daminozide (B-9)를 각 0, 1000 및 $2000mg{\cdot}L-1$ 농도로 처리하였으며, 적심처리는 4주차에 1회 처리 유무를 달리하였다. 모든 실험의 공통사항은 플라스틱 10호 화분에 원예상토를 충진한 후 200구 트레이에 종자를 셀당 4립씩 파종하여 생산된 유묘를 정식하였다. 연구의 결과, 기비처리는 초장, 마디수, 엽수가 증가하고, 절간장 및 측지수를 감소시켰으나, 유의적인 차이는 나타나지 않았다. 추비 처리는 농도가 높을수록 엽수가 증가하는 경향을 보였으며, $2000mg{\cdot}L-1$에서 무처리에 비해 유의적으로 많았다. 차광정도에 따른 긴산꼬리풀의 생육은 무차광 조건에서 생육이 가장 양호하였다. 차광률이 증가할수록 전반적인 생육이 억제되고, 개화율도 크게 감소하는 결과를 보였다. 왜화제 처리별로는 종류에 관계없이 전반적인 생육이 억제 되었다. Diniconazole 및 daminozide의 농도가 높을수록 초장이 억제되는 경향을 보였으나, 측지수, 마디수, 엽수도 감소하여 관상적 이용에 적합하지 않다. 적심을 할 경우 전반적인 생육은 큰 차이가 나타나지 않았으며, 측지수가 감소하였다. 따라서, 긴산꼬리풀의 분화생산을 위한 재배기술은 기비는 처리하지 않고, 추비는 $2000mg{\cdot}L-1$로 시비하며, 광이 충분한 곳에서 재배하는 것이 효과적이다. 또한, 왜화제와 적심은 일반적으로 줄기신장을 억제하고 마디수와 엽수를 증가시켜 관상적 이용효과가 있는 것으로 알려져 있지만 본 연구에서는 반대의 결과를 보여 처리 하지 않는 것이 효과적인 것으로 판단된다.