• Title/Summary/Keyword: 김철환

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Assessment of Natural Environment - I. Selection of Plant Taxa - (자연환경 평가 - I.식물군의 선정 -)

  • 김철환
    • Korean Journal of Environmental Biology
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    • v.18 no.1
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    • pp.163-198
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    • 2000
  • This study aim was to propose plant taxa as an alternative proposal for the purpose of solving many kinds of difficulties occurred in the floral item of environmental impact assessment (EIA) used to date. Plant taxa presented here for environmental assessment can probably be useful in understanding the excellence of natural environment to specific regional space as well as in determining the order of priority as to species preservation. These taxa, selected by the floristic analysis with distributional range, can be classified into five degrees: the fifth degree, characterized by the discontinuous and/or isolated distribution, includes 83 taxa, 76 genera, 41 families; the fourth degree, occurred in only one subprovince as southern and northern plants, includes 314 taxa, 217 genera, 78 families; the third degree, two subprovinces as southern and northern plants, includes 307 taxa, 223 genera, 93 families; the second degree, generally distributed more than 1,000 meter in altitude, a whole subprovince, includes 109 taxa, 92 genera, 43 families; the first degree, at least three subprovinces, includes 258 taxa, 207 genera, 91 families. The taxa mentioned here environmental assessment, therefore, summed up to 1,071 taxa, 559 genera, 142 families from the Korean flora. It is suggested that the taxa belonging to the fifth degree might structually have by far extinctive possibility in respond to environmental damage comparing with other degrees, simply because these have narrow distributional ranges. [Floristics, Korean flora, Geographical distribution, Natural environment, Environmental assessment, Plant taxa].

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Quality Characteristics of Unshiu Orange and Pear Packaged with Paper Incorporated with Antimicrobial Agents (항균소재를 함유한 포장재로 포장한 밀감과 배의 저장중 품질 특성 변화)

  • Park, Woo-Po;Jung, Jun-Ho;Cho, Sung-Hwan;Kim, Chul-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.10
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    • pp.1715-1719
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    • 2004
  • In order to help the preservation of the unshiu orange and pear, antimicrobial paper incorporating grapefruit seed extract and zeolite was applied to pack fruits. Unshiu orange was packed in a box (24${\times}$24${\times}$22 cm) attached with antimicrobial paper and then stored respectively at l$0^{\circ}C$. Pears were wrapped individually before storage at l$0^{\circ}C$. During the storage, weight loss, pH, total acidity, soluble solid content, microbial load and decay were measured as quality indices. Steady pH increase in unshiu orange was observed to slightly decrease total acidity during the storage with little difference between the packaging treatments. The microbial loads of total aerobic bacteria, and yeast/mold counts were suppressed during storage by the antimicrobial paper packaging, which also contributed to reducing the decayed unshiu orange. Limited reduction of total aerobic bacteria and yeast/mold counts was observed only for initial storage period for the pears wrapped with 9 and 12% antimicrobial agent-added papers. Antimicrobial paper was useful for the reduction of microbial load in unshiu orange and pear without other quality deterioration.

Effects of medium supplemented with persimmon peels on antioxidant activity of Pleurotus eryngii (감과피 첨가배지가 큰느타리 버섯의 항산화활성에 미치는 영향)

  • Kim, Chul Hwan;Kim, Hye Soo;Park, Hyun Young;Cheong, Jong-Chun;Lee, Chan-Jung;Kong, Won-Sik;Cho, Soo Jeong
    • Journal of Mushroom
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    • v.15 no.4
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    • pp.210-215
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    • 2017
  • In this study, we investigate the potential use of persimmon peels (PP) in mushroom culture medium for the production of functional mushrooms. Pleurotus eryngii was cultivated in medium supplemented with PP (SMPP) at the following concentrations: 0% SMPP (control), 5% SMPP, 10% SMPP, 15% SMPP, 20% SMPP, or 30% SMPP. The total polyphenol content, DPPH radical scavenging ability, ABTS cation scavenging ability, and reducing power of P. eryngii cultivated in SMPP were investigated. P. eryngii cultured in 20% SMPP produced the highest values for all four measurements. Total polyphenol content, DPPH radical scavenging activity, ABTS cation scavenging ability, and reducing power all increased upon the addition of PP. Based on our results, we can conclude that persimmon peels are a highly valuable supplement for functional mushroom culture medium.

Development of BNR(Biological Nutrient Removal) Process Using Expanded Polystylene(EPS) Media (Expanded Polystylene(EPS) 여재를 이용한 BNR(Biological Nutrient Removal) 공정 개발)

  • Ryu, Hong-Duck;Min, Kyung-Kook;Lee, Jeong-Hun;Choi, Kyoung-Young;Lim, Heun-Eun;Kim, Chul-Hwan;Lee, Sang-Ill
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.1
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    • pp.1-6
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    • 2006
  • Biological nutrient removal(BNR) process used in this study, which was packed with EPS(expanded polystylene) media, has more many advantages in aspect of reducing hydraulic retention time(HRT) of the process and having less effect of temperature in the winter season than commercial process in the present. Bench-scale study was performed using domestic wastewater. In the results, it was observed that the T-N removal efficiencies in HRT 6 hr and HRT 4 hr were 55% and 51%, respectively, showing the small reduction (about 4%) of T-N removal efficiency according to shortening of HRT. In comparison of T-N removal characteristic in summer and winter seasons, it showed that T-N removal efficiencies in summer and winter seasons were 65% and 54%, respectively, showing the 11% lower T-N removal efficiency in winter season than in summer season due to deterioration of nitrification in winter season. In the studies of influent loading rate and C/N ratio, the process showed stable effluent quality under the condition of broad influent loading rate and C/N ratio. Therefore, it is anticipated that the developed process in this study could be applicable to small wastewater treatment plant in nutrient removal.

The flora of land plants (bryophytes and vascular plants) in Wangpicheon Ecosystem and Landscape Conservation Area (ELCA), Korea (왕피천 생태경관보전지역의 육상(비관속 선태식물 및 관속식물) 식물상)

  • LEE, Seung Hyuk;AHN, Jin-Kap;PARK, Seung Jin;KIM, Chul Hwan;CHOI, Seung Se
    • Korean Journal of Plant Taxonomy
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    • v.50 no.3
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    • pp.279-317
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    • 2020
  • This study presents the flora of nonvascular plants (bryophytes) and vascular plants within the Wangpicheon Ecosystem and Landscape Conservation Area in Korea. The time of the survey was divided into four seasons. The fieldwork was conducted for a total of 18 days from November of 2016 to October of 2017. The land plants in the Wangpicheon conservation area were identified as 758 taxa with 164 families, 455 genera, 698 species, 10 subspecies, 47 varieties and 3 forms. Among them, bryophytes amounted to 122 taxa and vascular plants numbered 636 taxa. Native plant amounted to 693 taxa. In total, there are 113 floristic target species in Korea, including one level V taxon, in this case Swertia wilfordii; six level IV taxa; 31 level III taxa; 38 level II taxa; and 37 level I taxa. There are nine endemic plants, including Sillaphyton (=Peucedanum) podagraria, which is the endemic genus; two rare plants; fifteen northern plants; and one southern plant as target plants adaptable to climate change. Also, there are forty-six naturalized plants and five ecosystem-disturbance organisms. The results of this study suggest several species that should be preserved and can serve as basic information with which to establish conservation and management plans for the Wangpicheon conservation area.

Quality Characteristics of Cherry Tomato and Unshiu Orange Packaged with Box Incorporated with Antimicrobial Agents (항균소재 함유 박스로 포장한 방울토마토와 밀감의 저장중 품질 특성)

  • Park Woo-Po;Kim Chul-Hwan;Cho Sung-Hwan
    • Food Science and Preservation
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    • v.13 no.3
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    • pp.273-278
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    • 2006
  • In order to help the preservation of the cherry tomato and unshiu orange, antimicrobial paper incorporating grapefruit seed extract and zeolite was applied to the package. Cherry tomato and unshiu orange were packed in a box (38x25x20 cm) attached with antimicrobial paper and then stored respectively at $5^{\circ}C$. During the storage, weight loss, pH total acidity, soluble solid content microbial load and decay ratio were measured as quality indices. pH increase in cherry tomato was observed until 20 days, and decreased with litle difference between the packaging treatments thereafter pH and total acidity decrease in unshiu orange were shown till 30 days, and abrupt change was revealed by 40 days. This was due to physiological disorders. The microbial loads of total aerobic bacteria, and yeast/mold count were suppressed during storage by the box packaging incorporated with antimicrobial agents, which also contributed to reducing the decayed cherry tomato and unshiu orange. Antimicrobial paper was useful fur the reduction of microbial load in cherry tomato and unshiu orange pear without other quality deterioration.

STUDY ON EXPRESSION OF GLYCOSAMINOGLYCAN IN ADENOID CYSTIC CARCINOMA (선양낭성암종(Adenoid Cystic Carcinoma)에서의 Glycosaminoglycan의 발현에 관한 연구)

  • Son, Chang-Won;Kim, Kyung-Wook;Kim, Chul-Hwan
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.30 no.4
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    • pp.271-281
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    • 2004
  • Adenoid cystic carcinoma is malignant tumor in salivary gland, and its behavior is very invasive. Of all malignant tumor adenoid cystic carcinoma is occured in frequency of 4.4% in major salivary gland, and 1.29% in minor salivary gland. Histopathologically, adenoid cystic carcinoma is characterized by a cribriform appearance, and tubular form and solid nest type tumor can be seen. The tumor cell structure composed of modified myoepithelial cell, and basaloid cell. Extracellular matrix of this tumor cell contains variable ground substance with basement membrane component. Basement membrane matrix composed of collagen fibers, glycoproteins, proteoglycans, and its function is well known that it participate in differentiation, proliferation, and growth of tumor cell. Basement membrane molecule is essential for invasion of peripheral nerve, blood vessel, skeletal muscle in tumor cell of adenoid cystic carcinoma. In many studies, the tumor cell of adenoid cystic carcinoma containing modified myoepithelial cell participate in synthesis of proteoglycan. In this study, tissue sample of adenoid cystic carcinoma of human salivary gland were obtained from 15 surgical specimen, and all specimen were routinely fixed in 10% formalin and embedded. Serial $4-{\mu}m$ thick sections were cut from paraffin blocks. the histopathologic evaluation was done with light microscopy. And, the immunohistochemical staining, characteristics of glycosaminoglycan were observed. For biochemical analysis of glycosaminoglycan, isolation of crude glycosaminoglycan from tumor tissue and Western bolt analysis were carried out. With transmission electomicroscopy, tumor cell were observed. Biologic behavior of adenoid cystic carcinoma was observed with distribution and expression of basement membrane of glycosaminoglycan in tumor cells, The results obtained were as follows: 1. In immunohistochemical study, chondroitin sulfate is postively stained in tumor cell and interstitial space, dermatan sulfate is weakly stained in ductal cell. But keratan sulfate is negatively stained. 2. In immunohistochemical study, heparan sulfate is strong positive stained in tumor cell and basement membrane, especially in invasion area to peripheral nerve tissue. 3. In transmission electromicroscpic view, the tumor cells are composed modifed myoepithelial cells, and contains many microvilli and rough endoplasmic reticulum. 4. In Western blot analysis, the expression of glycosaminoglycan is expressed mostly in heparan sulfate. From the results obtained in this study, tumor cell of adenoid cystic carcinoma is composed modified myoepithelial cell, and glycosaminoglycan of basement membrane molecule of heparan sulfate and chondroitin sulfate mostly participate in the development and invasiveness of adenoid cystic carcinoma by immunohistochemical study and western blot analysis.

A COMPARATIVE STUDY ON POST-OPERATIVE ANALGESIC EFFECT FOR PATIENT-CONTROLLED AND INTRAMUSCULAR ANALGESIA IN MANDIBULAR FRACTURE PATIENTS (하악골 골절환자에서 술후 자가통증조절장치와 근주용 진통제의 효과에 관한 비교)

  • Lee, Seok-Jae;Kim, Kyung-Wook;Kim, Chul-Hwan
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.28 no.1
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    • pp.42-48
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    • 2006
  • Open reduction and rigid internal fixation is the most popular treatment method for maxillofacial fracture patients, and it is unevitable that postoperative pain can be developed. Many surgeons including oral & maxillofacial surgeons have made constant efforts to decrease postoperative pain. This study is a comparison of postoperative analgesia and intramuscular analgesia in patients with mandibular fractures. In this study, twenty-one patients (Experimental group) were randomly selected and they were injected with IV patient-controlled analgesia (PCA; Walkmed$^{(R)}$, USA). For control group another twenty-one patients were injected with intramusclar non-steroid anti-inflammatory drugs (Rheoma$^{(R)}$, Samsung Pharm. Co.). And then, we measured visual analogue scale (VAS) scores from first postoperative day to second day at regular time interval. The following results were uptained; 1. In patient group who with open reduction and rigid internal fixation, there was significant difference of postoperative analgesic effect during the first postoperative day(p<0.05). 2. In patient group with over 90 minutes surgery time, there was significant difference of postoperative analgesic effect during the first and second postoperative day when compared between experimental group and control group(p<0.05). 3. In patient group with less than 90 minutes surgery time, there was no significant difference of postoperative analgesic effect during the first and second postoperative day when compared between experimental group and control group(p>0.05). 4. In patient group with surgery of open reduction using rigid internal fixation at single fractured site, there was no significant difference of postoperative analgesic effect during the first and second postoperative day when compared between experimental group and control group(p>0.05). 5. In patient group with surgery at two fractured sites, there was significant difference of postoperative analgesic effect during the first postoperative day when compared between experimental group and control group(p<0.05). As mentioned above, it suggest that patient-controlled analgesia is more effective for postoperative pain relief than intramuscular injection in patients with rigid internal fixation by open reduction after mandibular fracture occurred. Especially, it is considered that in patient with more than 90 minutes surgery time or in cases with multiple fractured sites had more effective results with PCA therapy than conventional intramuscular analgesics.

GLYCOSAMINOGLYCAN EXPRESSION IN PLEOMORPHIC ADENOMAS OF THE SALIVARY GLAND (타액선 다형성 선종에서 Glycosaminoglycan의 발현)

  • Kim, Seong-Joo;Kim, Chul-Hwan;Kim, Kyung-Wook
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.28 no.1
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    • pp.1-12
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    • 2006
  • Pleomorphic adenoma is the most common benign tumor in salivary glands, and occurred in frequency of 60% in parotid gland tumors, and 50% in submandibular gland tumors, and 25% in sublingual gland tumors. Histopathologically, pleomorphic adenoma is composed of epithelial cells and mesenchymal tissues, and called 'mixed tumor' because of morphological divergency. The cell structures of luminal area are composed of polyhedral and cuboidal secretory epithelial cells and modified myoepithelial cells around it, and mesenchymal tissue is composed of some myoepithelial cells and stromal tissue. In stromal tissue, myxoid change, chondroid change, or hyalinization can be seen even if bone tissue. In many studies, tumor cells of pleomorphic adenoma containing modified myoepithelial cell participate in synthesis of glycosaminoglycans. In this study, tissue sample of pleomorphic adenoma of human salivary gland were obtained from 20 surgical specimens, and all specimens were routinely fixed in 10% formalin and embedded. Serial 4-8${\mu}m$ thick sections were cut from paraffin blocks. The histopathologic evaluation was done with light microscopy. And, with immunohistochemical staining, characteristics of glycosaminoglycan were observed. And, for biochemical analysis of glycosaminoglycan, isolation of crude glycosaminoglycan from tumor tissue and immuno-blot analysis were carried out. With transmission electromicroscopy, tumor cells and biologic behavior of pleomorphic adenoma were observed with distribution and expression of glycosaminoglycan in tumor cells, The results were obtained as follows: 1. In immunohistochemical study, chondroitin 4-sulfate is highly postively stained in myxoid stromal tissue, and chondroitin 6-sulfate is highly positively stained in chondroid mesenchymal tissue, both glycosaminoglycans are positively stained in non-luminal cell of ductal area. 2. Dermatan sulfate and keratan sulfate is positively stained in periductal non-luminal tumor cells. 3. In immunohistochemical study, heparan sulfate is weakly stained in luminal cells and non-luminal cells around duct, and chondroid mesenchymal tissue. 4. In transmission electromicroscopic view, the tumor cells are composed of modified myoepithelial cells, and contain many microfilaments and well developed rough endoplasmic reticulum. 5. In Immuno-Blot analysis, the expression of glycosaminoglycans is expressed mostly in chondroitin 6-sulfate and chondroitin 4-sulfate. From the results obtained in this study, tumor cells of pleomorphic adenoma are composed of modified myoepithelial cells, and glycosaminoglycans of chondroitin 4-sulfate and chondroitin 6-sulfate mostly participate in the development of pleomorphic adenoma, but dermatan sulfate, keratan sulfate and heparan sulfate glycosaminoglycans were expressed variably.

THE STUDY FOR HEMODYNAMIC CHANGE ON CORONAL APPROACH TO ZYGOMATICO-MAXILLARY COMPLEX FRACTURE (관골골절에 있어 관상피판 접근법시 혈액학적 변화에 대한 연구)

  • Kim, Hoon;Kim, Chul-Hwan;Yeo, Hwan-Ho;Kim, Su-Gwan
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.27 no.1
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    • pp.78-82
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    • 2001
  • Background : The coronal incision is versatile surgical approach to upper and middle region of the facial skeletal including the zygomatic arch. The advantages of coronal approach are minimal injury of facial tissue including facial nerve and satisfactory cosmetic result by hidden scar at hair. But wide exposure of scalp, its disadvantages are operation time and massive blood loss. Methods : Thirty patients undergoing elective surgery were divided 3 groups. Group I used only coronal approach, group II used coronal with subciliary approach and group III used coronal with subciliary and intraoral approach. And then retrospected of the preoperative, postoperative red blood cell count, hemoglobin(Hb), hematocrit, transfused red blood cell units and platelet cell units, and the amount of infused crystalloids and colloids, and postoperative hemovac count was estimated. Results: 1. Red blood cell count were decreased in all groups at immediated postoperation and decreased in all group of postoperative first day and decreased in group I, II but increased group III of postoperative third day. 2. Hemoglobin and hematocrit were decreased in all group at immediated postoperation and decreased in all group of postoperative first day and decreased in group I, II., but increased group III postoperative third day. 3. Platelet was decreased in all group at immediated postoperation, and decreased in group II, III but increased in group I of postoperative first day and decreased in group I but increased group II, III of postoperative third day. 4. Mean postoperative hemovac mean drainage group I of first day is $48.63{\pm}21.12ml$ and second day is $23.92{\pm}19.53ml$ and third day is $7.82{\pm}5.32ml$ and group II of first day $60.45{\pm}22.65ml$ and second day is $22.14{\pm}13.21ml$ and third day is $7.32{\pm}6.25ml$. III group of first day $58.16{\pm}10.13ml$ and second day is $21.27{\pm}11.72ml$ and third day is $7.13{\pm}4.90ml$. 5. Infusion of group I is mean PRC $1.08{\pm}0.91$ pint, FFP $1.03{\pm}0.75$ pint, crystalloid $2562.23{\pm}1345.53ml$ and group II is mean PRC $1.05{\pm}0.89$ pint, FFP $1.71{\pm}0.78$, crystalloid $2650.47{\pm}1096.36ml$ and group III is mean PRC $1.79{\pm}1.45$ pint, crystalloid $3295.43{\pm}1472.432ml$.

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