• Title/Summary/Keyword: 김준철

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Shoot Proliferation and Plant Regeneration from Suspension-Cultured Cells of Dianthus gratianopol (패랭이꽃속 Dianthus gratianopol의 현탁배양세포로부터 Shoot 증식과 식물체 재분화)

  • Kim Joon-Chul
    • Journal of Plant Biotechnology
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    • v.32 no.4
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    • pp.301-306
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    • 2005
  • Conditions for efficient organogenesis and plant regeneration from Dianthus gratianopol suspension cultured cells were established. Shoot-forming calli of glossy surface, pale green and knobby type were selected from leaf explant-derived calli and were suspension-subcultured every week in CP liquid medium with 1.0 mg/L 2,4-D and 0.5 mg/L BAP. Combinations of 1.0 mg/L 2,4-D and 0.5 mg/L BAP, and 1.5 mg/L 2,4-D and 0.5 mg/L BAP were effective for the induction of regenerative callus from the suspension cultured cell clusters. Multiple shoot primordia were initiated from the green spots of these regenerative callus and formed shoots on MS medium with 1.0 mg/L TDZ and 0.5 mg/L PAA. Shoot regeneration frequency (calli regenerating at least one shoot) was about 87%. For plant regeneration, proliferated shoots were excised and transferred to MS medium with 0.1 mg/L NAA for root initiation after 9 weeks of culture. The regenerants were potted in soil and formed the flowering buds and petals. Also, adventitious shoots were formed from the excised green shoot primordia of regenerative callus and these shoots proliferated successfully and regenerated to whole plants.

The Characteristics of the Fish Community in Yeongwol Hanbando Wetland (영월한반도습지의 어류군집 특성)

  • Han, Sang-Jin;Lee, Kwang-Yeol;Yoon, Young-Jin;Choi, Jae-Yoon;Kim, Joon Chul;Choi, Jae-Seok
    • Korean Journal of Environment and Ecology
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    • v.28 no.4
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    • pp.424-431
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    • 2014
  • Analysis of the fish community in Yeongwol Hanbando wetland in Korea was investigated from April to October 2012. During the survey period, total 33 species belonged to 10 families and 7,107 individuals. There were 18 Korean endemic species (52.94 %), including to Acheilognathus signifer. Also, the dominant species were Zacco koreanus at St. 1, 2, 3 & 5, as well Acheilognathus signifer at St. 6, and Pseudopungtungia tenuicorpus at St. 7, respectively, and these species were inhabited in the midstream. But at St. 4, Carassius auratus was dominance, so there appeared to characteristics of downstream or lentic habitat. Also, according to the similarity analysis on fish species and their individuals in each sites, they were divided into three major groups (A,B and C). Among them, group C (St. 4) showed the special inverse characteristics as a stagnant pond. unlike the river branch (group A) and anthropogenic disturbances receiving stream point (group B), Therefore, the ecosystem of Yeongwol Hanbando wetland is considered to very unique and these characteristics are not found in other stream ecosystems and it is confidently believed that the ecological value is very high.

The Clinical Evaluation of Anomalous Bronchi Arising From The Trachea and Main Bronchi (기관 및 주기관지에서 발생한 기관지기형의 임상적 고찰)

  • Kim, Jun Chul;Kim, Yeon Jae;Kang, Byung Jun;Youn, Young Deuk;Lee, Se Young;Kwon, Young Lan;Lee, Soo Ok;Jeong, Chi Yeong;Lee, Byung Ki
    • Tuberculosis and Respiratory Diseases
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    • v.59 no.6
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    • pp.664-669
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    • 2005
  • Objectives : To evaluate the clinical significance of abnormal bronchi originating from the trachea or main bronchi. Methods : 21 patients (male:female ratio, 13:8; mean age, 58.2 years, range 34-77), who were diagnosed with major tracheobronchial anomalies by bronchoscopy from January 2001 to March 2005, were enrolled in this study. The anomalous bronchi consisted of 13 tracheal bronchi and 8 cardiac accessory bronchus. The clinical features, bronchoscopic findings, and outcomes were analyzed retrospectively. Results : Common symptoms, including hemoptysis, cough and dyspnea, resulted from the underlying lung disease regardless of the bronchial anomalies. In this series of 13 tracheal bronchi, 7 cases originated from the trachea within 1cm of the carina (carinal type) and 6 cases originated at a higher level(tracheal type). Most patients had favorable outcome with conservative treatment for the underlying lung disease. Conclusion : Most tracheobronchial anomalies are found incidentally in the process of diagnosing lung disease. The clinical outcome of patients with a bronchial anomaly depends on the underlying lung disease.

Implementation of Front End Module for 2.4GHz WLAN Band (2.4GHz 무선랜 대역을 위한 Front End Module 구현)

  • Lee, Yun-Sang;Ryu, Jong-In;Kim, Dong-Su;Kim, Jun-Chul;Park, Jong-Dae;Kang, Nam-Kee
    • Journal of the Microelectronics and Packaging Society
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    • v.15 no.1
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    • pp.19-25
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    • 2008
  • In this paper, the front end module (FEM) was proposed for 2.4GHz WLAN band by LTCC multilayer application. The FEM was composed of power amplifier IC, switch IC, and LTCC module. LTCC module consists of output matching circuit and lowpass filter as Tx part, bandpass filter as Rx part. Design of output matching circuit for LTCC was used matching parameter from output matching circuit based on lumped circuit on the PCB board. The dielectric constant of LTCC substrate is 9. The substrate was composed of total 26 layers with each 30um thickness. Ag paste was used for the internal pattern as the conductor material. The size of the module is $4.5mm{\times}3.2mm{\times}1.4mm$. The fabricated FEM showed the gain of 21dB, ACPR of less than -31dBc first side lobe and Less than -59dBc second side lobe and the output power of 23Bm at P1dB.

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A temperature stable bandpass filter using dieletric-filled stepped impedance resonators (접합된 Stepped impedance resonator를 이용한 온도보상형 유전체 대역통과 필터)

  • Lim, Sang-Kyu;Kim, Jun-Chul;Kim, Duck-Hwan;Ha, Jong-Su;Oh, Chang-Heon;Sim, Hwa-Sup;An, Chul
    • Journal of the Korean Institute of Telematics and Electronics D
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    • v.35D no.2
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    • pp.78-85
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    • 1998
  • The design method of a temperature stable bandpass filter using dielectric coaxial resonators of with two dielectric ceramics with opposite signs of temperature coefficient of dielectric constant (${\tau}_{\epsilon}$) to compensate for each other in this method. $MgTiO_3$(${\tau}_{\epsilon}$=+99 ppm/${\circ}C$) as a positive ${\tau}_{\epsilon}$ material and Ba($Zn_{1/3}Nb_{2/3}$)$O_3$(${\tau}_{\epsilon}$=-77ppm/${\circ}C$) as a negative material were selected. The length of a SIR for the temperature stability was calculated according to the design method and the susceptance slope parameter of the SIR was obtained. A temperature stable bandpass filter using dielectric SIR's was designed, simulated and fabricated. The center frequency of this filter was 915 MHz and the pass bandwidth was 20 MHz. Temperature properties of this bandpass filter by simulations were compared with the measured results of the bandpass filter fabricated.

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Design of a LTCC Front End Module with Power Detecting Function (전력 검출 기능을 포함하는 LTCC 프런트 엔드 모듈 설계)

  • Hwang, Mun-Su;Koo, Jae-Jin;Koo, Ja-Kyung;Lim, Jong-Sik;Ahn, Dal;Yang, Gyu-Yeol;Kim, Jun-Chul;Kim, Dong-Su;Park, Ung-Hee
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.19 no.8
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    • pp.844-853
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    • 2008
  • This paper describes the design of a FEM(Front End Module) having power detection function for mobile handset application. The designed FEM consists of a MMIC(Monolithic Microwave Integrated Circuits) power amplifier chip, SAW Tx filter and duplexer, diode power detector and stripline matching circuit. An LTCC(Low Temperature Co-fired Ceramics) technology is adopted for miniaturized FEM. The frequency band is $824{\sim}869$ MHz which is the uplink Tx band of the CDMA mobile system. The size of designed FEM is $7.0{\times}5.5{\times}1.5\;mm^3$, which is an ultra-small size even though the power detector circuit is included. All sub-components of FEM have been developed and measured in advance before being integrated into FEM. The measured output power and gain are 27 dBm and 27 dB, respectively. In addition, the measured ACPR characteristics are 46.59 dBc and 55.5 dBc at 885 kHz and 1.98 MHz offset, respectively.

Implementation of an LTCC RF Front-End Module Considering Parasitic Elements for Wi-Fi and WiMAX Applications (기생 성분을 고려한 Wi-Fi와 WiMAX용 LTCC 무선 전단부 모듈의 구현)

  • Kim, Dong-Ho;Baek, Gyung-Hoon;Kim, Dong-Su;Ryu, Jong-In;Kim, Jun-Chul;Park, Jong-Chul;Park, Chong-Dae
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.21 no.4
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    • pp.362-370
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    • 2010
  • In this paper, a compact RF Front-end module for Wireless Fidelity(Wi-Fi) and Worldwide Interoperability for Microwave Access(WiMAX) applications is realized by low temperature co-fired ceramic(LTCC) technology. The RF Front-end module is composed of three LTCC band-pass filters, a Film Bulk Acoustic Resonator(FBAR) filter, fully embedded matching circuits, an SPDT switch for mode selection, an SPDT switch for Tx/Rx selection, and an SP4T switch for band selection. The parasitic elements of 0.2~0.3 pF are generated by the structure of stacking in the top pad pattern for DC block capacitor of SPDT switch for mode selection. These kinds of parasitic elements break the matching characteristic, and thus, the overall electrical performance of the module is degraded. In order to compensate it, we insert a parallel lumped-element inductor on capacitor pad pattern for DC block, so that we obtain the optimized performance of the RF Front-end module. The fabricated RF front-end module has 12 layers including three inner grounds and it occupies less than $6.0mm{\times}6.0mm{\times}0.728mm$.

Adventitious Bud Induction and Plant Regeneration from Mature Embryos in Larix leptolepis (낙엽송 (Larix leptolepis) 성숙배로부터 부정아 유도 및 식물체 재분화)

  • Kim, Yong-Wook;Kim, Joon-Chul;Youn, Yang;Noh, Eu-Rae;Son, Sung-Ho
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.4
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    • pp.251-257
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    • 1999
  • Adventitious buds were produced from the cultures of mature zygotic embryos of Larix leptolepis with the highest frequency of 91.7% in SH medium containing 1.0 mg/L zeatin. The most effective cytokinin combination for inducing adventitious buds was 1.0 mg/L zeatin + 1.0 mg/L thidiazuron (40.3%). The highest mean number of adventitious buds induced in 1.0 mg/L zeatin + 1.0 mg/L 2iP or 1.0 mg/L zeatin + 1.0 mg/L kinetin combined treatments. Elongation of the adventitious buds occurred the best on half strength LM salts medium, on which the buds elongated upto 27 mm. Also, the supplement of activated charcoal in medium suppressed the elongation of the adventitious buds. The highest frequency (23.3%) of rooting from elongated shoots was obtained on the medium containing 5.0 mg/L IBA and 0.2 mg/L NAA. The highest number (3.5) of roots was induced on the medium containing 5.0 mg/L IBA alone.

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Effect of Ethylene on Formations of Adventitious Roots, Trichomes, and Calli by NAA in Leaf segments of Arabidopsis thaliana (애기장대 잎 절편 배양시 NAA 농도에 따른 부정근, 모용 및 캘러스 형성에 미치는 에틸렌의 영향)

  • 한태진;홍종필;김준철;임창진;진창덕
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.4
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    • pp.259-264
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    • 1999
  • In order to study the role of ethylene on the formation of adventitious roots, trichomes and calli, the effects of 1-aminocyclopropane-l-carboxylic acid (ACC), ethephon, $CoCl_2$ and $AgNO_3$ were investigated in the leaf segments from ecotype Columbia of Arabidopsis thaliana. When the leaf segments were cultured on the media for forming adventitious roots (0.1 mg/L NAA), trichomes (2.0 mg/L NAA) and calli (10.0 mg/L NAA), and then each cultures was treated with 1-100 mg/L of ACC and ethephon, respectively. On the adventitious root-forming medium adventitious root formation was decreased, and trichomes were induced. And on the trichome-forming medium trichome formation was decreased, and calli were induced. In order hand each culture was treated with 1-100 mg/L of $CoCl_2$ and $AgNO_3$, respectively. On the adventitious root-forming medium adventitious roots was increased without trichome formation, and on the trichome-forming medium trichome formation was decreased, and adventitious roots were induced. However on the callus-forming medium treated with ACC, ethephon, $CoCl_2$ and $AgNO_3$, respectively, callus formation was inhibited and trichomes were induced in all cultures.

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Plant Regeneration and Mutagenesis from Organogenic Callus of Dianthus Distributed in Gangwon Province (강원지역 패랭이꽃속의 캘러스로부터 식물체 재분화와 돌연변이체 유발)

  • Chang, Mi-Young;Hong, Sung-Won;Kim, Joon-Chul
    • Journal of Plant Biotechnology
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    • v.30 no.1
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    • pp.73-80
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    • 2003
  • Useful Dianthus species were collected and selected from two native and seven foreign species distributed in Gangwon province. For in vitro breeding,. callus was induced from the explants of apical meristem, leaf, stem and the in vitro adventitious shoots on MS basal medium with 2.0 mg/L 2,4-D and 0.5 mg/L BA at 27$^{\circ}C$ under continuous light. After 3 weeks of culture, calli initiated the most highly from the leaf explants of D. chinensis Organogenic calli were able to be selected from the adventitious shoot-derived calli. For shoot regeneration, these organogenic calli were cultured on MS medium with the combination of 0.1 mg/L NAA+1.0 mg/L BA under continuous light. Multiple shoots were proliferated with low frequency (about 30%) from those adventitious shootderived calli. Also, shoots initiated directly from the adventitious shoot explants without callus formation at high frequency of 52% when cultured on N6 medium containing 0.1 mg/L NAA and 1.0 mg/L BA in D. gratianopol. Multiple shoots and plantlets grew well and rooted on MS medium supplemented with 0.1 mg/L NAA. Regenerants with well-developed roots were transferred to 8-cm pots containing vermiculite at 85% relative humidity and 27$^{\circ}C$ These plantlets were acclimatized in artificial soil mixture and transferred to the greenhouse for flowering with normal phenotypes. M28 Mutant line was selected with white flowers from 0.03M EMS-treated organogenic calli derived from in vitro adventitious shoot explants of D. chinensis and set seeds.