• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.50-50
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• 2023

Prostaglandin E2(PGE2), a major product of cyclooxygenase-2 (COX-2), plays an important role in the carcinogenesis of many solid tumors, including colorectal cancer. Because PGE2 functions by signaling through PGE2 receptors (Eps), which regulate tumor cell growth, invasion, and migration, there has been a growing amount of interest in the therapeutic potential of targeting Eps. In the present study, we investigated the role of EP4 on the effectiveness of cordycepin in inhibititing the migration and invasion of HCT116 human colorectal carcinoma cells. Our data indicate that cordycepin suppressed lipopolysaccharide (LPS)-enhanced cell migration and invasion through the inactivation of matrix metalloproteinases (MMP)-9 as well as the down-regulation of COX-2 expression and PGE2 production. These events were shown to be associated with the inactivation of EP4 and activation of AMP-activated protein kinase (AMPK). Moreover, the AMPK inhibitor, compound C, as well as AMPK knockdown via siRNA, attenuated the cordycepin-induced inhibition of EP4 expression. Cordycepin treatment also reduced the activation of CREB. These findings indicate that cordycepin suppresses the migration and invasion of HCT116 cells. Through modulating EP4 expression and the AMPK-CREB signaling pathway. Therefore, cordycepin has the potential to serve as a potent anti-cancer agent in therapeutic strategies against colorectal cancer metastasis.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.51-51
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• 2023

The world-wide rate of obesity is increasing continuously, representing a serious medical threat since it is associated with a variety of diseases including type 2 diabetes, cardiovascular disease, and numerous cancers. Sophora japonicais used as a traditional herb for medicinal purposes in eastern Asia. However, the anti-obesity effects of S. japonicafruit have not been explored. The aim of this study is to investigate the inhibition of adipocyte differentiation and adipogenesis by an ethanol extract of S. japonicafruit (EESF) in 3T3-L1 pre-adipocytes. Our results demonstrate that EESF suppressed the terminal differentiation of 3T3-L1 pre-adipocytes in a dose-dependent manner, as confirmed by a decrease in lipid droplet number and lipid content through Oil Red O staining. EESF significantly reduced the accumulation of cellular triglyceride, which was associated with a significant inhibition of the levels of pro-adipogenic transcription factors, including PPARγ, C/EBPα and C/EBPβ. In addition, EESF potentially down regulated the expression levels of adipocyte-specific proteins, including aP2 and leptin. In particular, EESF treatment effectively enhanced the activation of the AMPK signaling pathway; however, the co-treatment with compound C, an inhibitor of AMPK, significantly restored the EESF-induced inhibition of pro-adipogenic transcription factors and adipocyte-specific genes. These results indicate that EESF may exert an anti-obesity effect by controlling the AMPK signaling pathway, suggesting that the fruit extract of S. japonica may be a potential anti-obesity agent.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.17-17
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• 2023

Chrysanthemum zawadskii (C. zawadskii) is used in traditional East Asian medicine for the treatment of various diseases, including inflammatory disease. However, it has remained unclear whether extracts of C. zawadskii inhibit inflammasome activation in macrophages. The present study assessed the inhibitory effect of an ethanol extract of C. zawadskii (CZE) on the activation of the inflammasome in macrophages and the underlying mechanism. Bone marrow[-derived macrophages (BMDMs) were obtained from wild-type C57BL/6 mice. The release of IL-1β and lactate dehydrogenase in response to nucleotide-binding oligomerization domain-like receptor (NLR) family pyrin domain containing 3 (NLRP3) inflammasome activators, such as ATP, nigericin and monosodium urate (MSU) crystals, was significantly decreased by CZE in lipopolysaccharide(LPS)-primed BMDMs. Western blotting revealed that CZE inhibited ATP-induced caspase-1 cleavage and IL-1β maturation. To investigate whether CZE inhibits the priming step of the NLRP3 inflammasome, we confirmed the role of CZE at the gene level using RT-qPCR. CZE also downregulated the gene expression of NLRP3 and pro-IL-1β as well as NF-κB activation in BMDMs in response to LPS. Apoptosis associated speck-like protein containing a caspase-recruitment domain (CARD) oligomerization and speck formation by NLRP3 inflammasome activators were suppressed by CZE. By contrast, CZE did not affect NLR family CARD domain containing protein 4 (NLRC4) or absent in melanoma 2 (AIM2) inflammasome activation in response to Salmonella typhimurium and poly(dA:dT) in LPS-primed BMDMs, respectively. The results revealed that three key components of CZE, namely linarin, 3,5-dicaffeoylquinic acid and chlorogenic acid, decreased IL-1β secretion in response to ATP, nigericin and MSU. These findings suggest that CZE effectively inhibited activation of the NLRP3 inflammasome.

• Journal of Venture Innovation
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• v.6 no.1
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• pp.17-34
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• 2023

This study tried to find a way to increase major satisfaction to overcome the difficulties of college due to the recent decrease in the number of educated population. In particular, problem-solving ability and digital literacy, which are essential in the era of the 4th industrial revolution, were set as influencing factors and mediators and tried to verify them. Therefore, in order to find out the effect of problem-solving ability on major satisfaction and the mediating effect of digital literacy, a study was conducted targeting junior college secretarial majors. A total of 193 questionnaires were analyzed, and regression analysis was performed to verify the hypothesis, and bootstrapping was performed using the SPSS PROCESS macro model 4 to verify the significance of the mediating effect. The results of the study are as follows. First, it was found that problem-solving ability had a positive(+) effect on major satisfaction. Second, problem-solving ability was found to have a positive(+) effect on all four sub-factors of digital literacy. Third, as a result of analyzing the mediating effect of digital literacy in the relationship between problem-solving ability and major satisfaction, among the four sub-factors of digital literacy, information literacy, critical understanding, and information analysis ability completely mediated between problem-solving ability and major satisfaction. has been shown to be effective. On the other hand, the ability to comply with norms was found to have no mediating effect. In this study, it was verified that problem-solving ability affects major satisfaction, and it was found that it is necessary to make various efforts to cultivate problem-solving ability. In addition, based on these research results, suggestions for preparing practical measures to increase major satisfaction were suggested.

• Journal of Family Resource Management and Policy Review
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• v.27 no.3
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• pp.21-37
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• 2023

This study examines the living conditions and life satisfaction of single-person households according to their life cycle. The survey was conducted from August to October 2022, and respondents were single-person households from Kimpo, South Korea. The respondents were categorized to three groups namely, young, middle-aged, and older adults by life cycle, and items regarding their sociodemographic background, personal life, family relations, and life satisfaction were included in the analysis. Descriptive statistics, Chi-square analysis, analysis of variance, and multiple regression analysis were performed. The key findings were as follows. First, significant differences were found according to life cycle in the respondents' diet, clothing, housing, financial and home management, self-care, and leisure life. Second, the variables that significantly affected the level of life satisfaction of single-person households were the life cycle with young adults showing a higher level of satisfaction; having a family member to care; living alone voluntarily, discrimination experience; community awareness; and ties with the local community. Based on the results, it is clear that single-person households have different needs and problems in each stage of life cycle. Thus, to reflect the different experiences and needs of single-person households by life cycle, tailored policy and programs should be provided for young, middle-aged, and older adult single-person households.

• Journal of Korea Port Economic Association
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• v.39 no.3
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• pp.91-105
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• 2023

This paper identified the difference in perception of the consignment system between the freight company and the consigned vehicle owners, derived factors that urgently need to improve the difference in perception, and presented the basis for establishing a management model based on this. A management model based on the difference in perception of consigned borrowers was established, and research hypotheses were established to conduct reliability, validity, and confirmatory factor analysis, path analysis, and mediation effect verification. As a result of the analysis, out of 58 questions, 56 of the total 65 questions showed a difference in perception of the consignment system between trucking companies and consigned vehicle owners, and the questions were input as management model factors. As a result of conducting confirmatory factor analysis, path analysis, and mediating effect verification using only recognition data, 5 hypotheses were adopted, and it was analyzed that the factor had no mediating effect. It can be analyzed that the appropriateness of the compensation system affects the improvement of each other's relationship, and the improvement of each other's relationship leads to the improvement of transportation services. However, since the mediating effect was rejected because it was not significant, this effect should not be exaggerated.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.34 no.2
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• pp.263-279
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• 2024

With the increasing trend of Cloud migration, security threats in the Cloud computing environment have also experienced a significant increase. Consequently, the importance of efficient incident investigation through log data analysis is being emphasized. In Cloud environments, the diversity of services and ease of resource creation generate a large volume of log data. Difficulties remain in determining which events to investigate when an incident occurs, and examining all the extensive log data requires considerable time and effort. Therefore, a systematic approach for efficient data investigation is necessary. CloudTrail, the Amazon Web Services(AWS) logging service, collects logs of all API call events occurring in an account. However, CloudTrail lacks insights into which logs to analyze in the event of an incident. This paper proposes an automated analysis framework that integrates Cloud Matrix and event information for efficient incident investigation. The framework enables simultaneous examination of user behavior log events, event frequency, and attack information. We believe the proposed framework contributes to Cloud incident investigations by efficiently identifying critical events based on the ATT&CK Framework.

• Journal of the Korea Institute of Military Science and Technology
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• v.27 no.4
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• pp.507-515
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• 2024

Microbial forensics is a scientific discipline for analyzing evidence related to biological crimes by identifying the origin of microorganisms. Multiple locus variable number tandem repeat analysis(MLVA) is one of the microbiological analysis methods used to specify subtypes within a species based on the number of tandem repeat in the genome, and advances in next generation sequencing(NGS) technology have enabled in silico anlysis of full-length whole genome sequences. In this paper, we analyzed unknown samples provided by Robert Koch Institute(RKI) through The United Nations Secretary-General's Mechanism(UNSGM)'s external quality assessment exercise(EQAE) project, which we officially participated in 2023. We confirmed that the 3 unknown samples were B. anthracis through nucleic acid isolation and genetic sequence analysis studies. MLVA results on 32 loci of B. anthracis were analysed by using genome sequences obtained from NGS(NextSeq and MinION) and Sanger sequencing. The MLVA typing using short-reads based NGS platform(NextSeq) showed a high probability of causing assembly error when a size of the tandem repeats was grater than 200 bp, while long-reads based NGS platform(MinION) showed higher accuracy than NextSeq, although insertion and deletion was observed. We also showed hybrid assembly can correct most indel error caused by MinION. Based on the MLVA results, genetic identification was performed compared to the 2,975 published MLVA databases of B. anthracis, and MLVA results of 10 strains were identical with 3 unkonwn samples. As a result of whole genome alignment of the 10 strains and 3 unknown samples, all samples were identified as B. anthracis strain A4564 which is associated with injectional anthrax isolates in heroin users.

• Journal of the Korean Society of Radiology
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• v.82 no.4
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• pp.994-999
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• 2021

Late recurrence over 10 years after surgery and endobronchial metastasis are some of the specific biological behaviors of renal cell carcinoma (RCC). The current report describes a case of solitary endobronchial metastasis at a subsegmental bronchus that developed 20 years after curative nephrectomy for RCC. A 71-year-old male was admitted to our hospital for pneumonia. Chest radiography showed multifocal ill-defined nodular opacities in the right lower lung zone, suggesting pneumonia. Subsequent chest CT confirmed pneumonic infiltration in the right lung. However, a 4.3-cm, well-defined, elongated mass with a branching pattern was also identified in the right lower lobe, and a right nephrectomy scar was detected on the covered upper abdomen. The patient had undergone right nephrectomy 20 years ago due to clear cell RCC. After right lower lobectomy, the postoperative pathological diagnosis was endobronchial metastatic clear cell RCC. Endobronchial metastasis should be considered in a patient with a history of RCC who presents with a suspected endobronchial tumor, even decades after curative surgery.

• Journal of Family Resource Management and Policy Review
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• v.28 no.1
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• pp.13-25
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• 2024

The rate of single-person households has been increasing all over the world, and there has been a particularly rapid increase in them in Korea. Single-person households show unique and various characteristics related to the reasons for becoming a single-person household, gender, life cycle, and so on. Thus, research needs to focus on the specific groups of single-person households in order to provide tailored policies and programs. This study segmented single-person households in three groups based on life cycle: young, middle-aged, and older adults. Differences in the level of healthy lifestyle, as well as factors affecting that, were investigated according to the groups. The data were collected in 2022, with 237 respondents from single-person households in Kimpo. Descriptive statistics, t-test, ANOVA, and multiple regression analysis were conducted. The overall level of healthy lifestyle was found to be significantly higher for young adults compared to older adults. Results from multiple regression show that significant factors related to the healthy lifestyle of single-person households were gender, educational attainment, whether becoming a single-person household was voluntary, and whether the respondents had experienced discrimination as a single-person household. Significant factors differed by the sub-categories of the healthy lifestyle scale. This study has implications related to discovering differences in the level of healthy lifestyles of single-person households through examining the factors affecting it according to life cycle.