• Korean Journal of Food Science and Technology
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• v.35 no.3
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• pp.488-492
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• 2003

Yeast cell wall mutant, Saccharomyces cerevisiae IS2 was screened by the NTG treatment of Saccharomyces cerevisiae KCTC 7911. The mutant was highly resistant to zymolase, which specifically degrades ${\beta}$-1,3-D-glucose chain of ${\beta}$-glucan and mechanical disruption by glass beads. These phenomena demonstrate that the yeast mutant has cell wall structure different from the wild-type. The ${\beta}$-glucan of yeast mutant and wild-type strains was recovered by sequential extraction with NaOH. The injection of ${\beta}$-glucan into the abdominal cavity of mouse resulted in an increase in the number of peritoneal immune cells, NO (nitric oxide) production, and phagocytic activity of macrophage. The number of immune cells was found to be $3.90{\times}10^6\;cells/10\;mL$ and $5.48{\times}10^6\;cells/10\;mL$ with the wild-type and mutant ${\beta}$-glucan, respectively. The effect on the NO production and phagocytic activity of mutant ${\beta}$-glucan were 1.69 and 1.43-fold higher than those of wild-type. These results indicate that the immuno-stimulating activity of alternated ${\beta}$-glucan from mutant yeast is higher than that of wild-type.

• Korean Journal of Microbiology
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• v.35 no.4
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• pp.296-301
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• 1999

A Bacillus strain from Korean soil was isolated and identified to be Bacillus licheniformis B1 through various biochemical tests, VITEK, and MIDI system analysis. The strain produced extracellular amylase and protease. Whether or not the strain can perform Chungkookjang fermentation with autoclaved soybean and Kanjang fermentation was determined in this study. In Chungkookjang fermentation, browining materials of strong anti-oxidant increased 8-fold, and 2-fold in Kanjang, compared with initiation material for fermentation. Maximal protease activity in Chungkookjang was observed one day after inoculation. Protease activities in Kanjang decreased to the half, and then maintained constant values during fermentation, probably due to the inhibitory effect of salt on protease activities. High molecular mass of nucleic acids was identified in Chungkookjang and Kanjang. Since the nucleic acids were not observed in autoclaved soybean, they seem to be originated from B. licheniformis B1. This study demonstrated successive fermentation of Chungkookjang and Kanjang by B. licheniformis B1 isolated from nature, and suggested possible development of food rich in browing and nucleic acids.

• Korean Journal of Poultry Science
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• v.33 no.3
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• pp.211-215
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• 2006

This study was carried out to determine whether genistein implants in laying hens could be transferred into their eggs. 250 mg genistein pellet was implanted two or four subcutaneously in the neck of laying hens. The contents of genistein in egg yolk transferred were analyzed with HPLC-MS. In 500 mg genistein pellets, it was detected as 395 ng/egg yolk on the day 18 after implanting and maintained as 546 ng/egg yolk after the day 59. In 1,000 mg, genistein was detected as 240 ng/egg yolk on the day 13, as 514 ng/egg yolk on the day 30 and maintained over 59 days. In conclusion, the direct genistein implants could be more twenty times efficiently transferred to egg yolks than dietary supplement.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2012.10a
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• pp.99-102
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• 2012

An image scaler is a IP used in a image processing block of display devices to adjust image size. Proposed image scaler adopts line memories instead of a conventional method using a frame memory. This method reduced hardware resources and enhanced data precision by using shift operations that number is multiplied by $2^m$ and divided again at final stage for scaling. Also image scaler increased efficiency of IP by using serial divider to calculate parameters by itself. Parameters used in image scaling is automatically produced by it. Suggested methods are designed by Verilog HDL and implemented with Xilinx Vertex-4 XC4LX80 and ASIC using TSMC 0.18um process.

• Ecology and Resilient Infrastructure
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• v.10 no.4
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• pp.250-257
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• 2023

Tree in river have environmental functions such as ecosystem preservation and flood control functions that protect the riverbank. On the other hand, excessive tree development can have the negative effect of fixing the sand bar and reducing the cross-sectional area. Nays2D simulation results performing two flow conditions (average dam operation discharge and two-year frequency discharge) and four tree density conditions (current, zero, low, high tree density) used as input data for PHABSIM to calculate WUA (Weighted Usable Area). The results show that riverbed changes occur more significantly in the zero tree density than presence of trees, which could have a positive impact on the biological habitat environment of Zacco platypus.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.4
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• pp.430-435
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• 2009

The effects of turmeric and fermented turmeric by Bacillus natto on antioxidant activities, liver function recovery of acute hepatotoxicity mice, and serum lipid parameters in high fat diet fed mice were investigated. In the results of antioxidant activity by DPPH method, fermented turmeric had higher antioxidative activity than turmeric. Acute hepatotoxicity was induced by 0.5 mL of carbon tetrachloride ($CCl_4$) per kg of mice. Unlike turmeric, fermented turmeric significantly reduced the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) after 5 days compared to the controls with 0.5% methyl cellulose (p<0.05). In addition, higher recovery of liver damage by $CCl_4$ was observed in mice with fermented turmeric than with turmeric. High fat (20%) diet fed mice were divided into 4 groups to investigate the effects of turmeric and fermented turmeric on serum lipid parameters: C (vehicle), TuL (low dose (80 mg/kg) with turmeric), TuH (high dose (160 mg/kg) with turmeric), FTuL (low dose with fermented turmeric), and FTuH (high dose with fermented turmeric). The levels of LDL-cholesterol and HDL-cholesterol were significantly reduced and increased in FTuL, FTuH and TuH groups compared to the C group, respectively. However, there was no significant change in triglyceride levels by either turmeric or fermented turmeric compared to those by control. Collectively, these results strongly suggest that fermented turmeric by Bacillus natto could be used as a functional food for enhancement of health with better consumer acceptance.

• Journal of Periodontal and Implant Science
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• v.37 no.sup2
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• pp.311-323
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• 2007

Receptor activation of nuclear factor ${\kappa}$ B ligand (RANKL)은 파골세포의 분화와 기능에 중요한 역할을 하는 단백질로 이들 물질의 조절에는 p38 MAP kinase가 관여한다. 그러나 치주인대 섬유모세포에서 RANKL 발현 시 p38 MAP kinase의 역할은 잘 알려져 있지 않다. 이에 이번 연구는 마우스 치주인대 섬유모세포의 $IL-1{\beta}-induced$ RANKL 발현과정에서 p38의 역할을 규명하고자 하여 다음과 같은 결과를 얻었다. 마우스 치주인대 섬유모세포에 $IL-1{\beta}$ (1ng/ml)의 자극은 수용성 RANKL의 합성을 증가시켰다. 수용성 RANKL의 합성은 p38 MAP kinase 억제제인 SB203580에 의해 농도 의존적으로 억제되었으나 다른 MAP kinase 억제제인 SP600125, JNK 억제제와 PD98059, ERK 억제제에 의해서는 수용성 RANKL의 합성이 조절되지 않았다. NF-kB 억제제에 의해서도 수용성 RANKL의 합성이 억제되지 않았다. RANKL 유전자의 발현은 $IL-1{\beta}$로 자극 시에는 대조군에 비해 약 5배의 발현 증가를 보였으나 SB203580으로 전처치 시 $IL-1{\beta}$ (1ng/ml)로 자극시보다 약 1.5배의 감소를 보였다. 그러나 SP600125, PD98059, 및 NF-kB 억제제로 전처치한 경우에는 $IL-1{\beta}$로 자극한 경우와 비슷한 수준을 보였다. $IL-1{\beta}$로 자극 시 RANKL 유전자의 반감기가 90분 이었으나 SB203580로 전처치 후 $IL-1{\beta}$로 자극 시 RANKL 유전자의 반감기는 60분으로 감소하였다. Cycloheximide 전처리 시 SB203580에 의한 RANKL 유전자 발현 억제가 관찰되지 않았다. 단백질 분석결과 p38 MAP kinase의 인산화 활성은 30분까지 증가하였으나 그 이후 감소하여 2시간째에는 그 발현이 미약하였다. SB203580로 전처치 후 $IL-1{\beta}$로 자극 시 p38 MAP kinase의 인산화 활성이 감소하였다. 이상의 결과는 p38 MAP kinase가 RANKL 유전자 조절에 중요한 역할을 담당하고 있음을 시사한다.

• 한국벤처창업학회:학술대회논문집
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• 2017.04a
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• pp.39-39
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• 2017

정부는 대학발 창업에 대한 기본계획을 확정하고 그동안 산재되어 있는 창업지원기능을 대학중심으로 전환하고 있다. 본 연구는 기업가 정신을 확산하고 창업성과와 연계하기 위한 대학의 창업교육과정 및 창업지원체계 구축이 창업성과에 미치는 영향을 종단적으로 분석을 시도한다. K대학은 1998년 지역 내에서 가장 먼저 창업보육센터로 지정을 받았고 이후 2011년 창업선도대학, 2012년 LINC사업, 2013년 사관학교식 창업선도대학 및 2014년 글로벌창업대학원 지원사업 선정 등 각종 정부지원사업을 통해 창업인프라를 구축하여 왔다, K대학은 학부생, 대학원생을 대상으로 창업수준 4단계, 기술분야별 교과과정 체계를 구축하였다. 또한 아이디어 도출부터 기업설립까지 창업 과정을 실질적으로 경험해 볼 수 있는 창업동아리와 이를 지원하는 비교과 프로그램과 창업친화형학사제도가 갖추어져 있다. 또한 창업 활동을 효과적으로 지원하기 위해 대학 내에 산재한 창업지원기관을 통합하고 본부 조직화하였다. K대학은 이를 통해 창업교과 수강학생 수 및 창업동아리 수에서 2배 수준의 증가와 창업자 수는 2013년에 비해 7배의 성장을 이루었다. K대학의 창업교육과 창업지원체계 및 창업성과를 종단적으로 분석하여 대학에서의 창업시스템이 단계별로 구축될 필요성과 대학 내 구성원의 창업에 대한 인식전환과 성과도출까지 적응기간의 필요성을 도출하였다.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.326-335
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• 2015

Citrus is an economically important fruit tree with the largest amount of fruit production in the world. It provides important nutrition such as vitamin C and other health-promoting compounds including its unique flavonoids for human health. However, it is classified into the most difficult crops to develop new cultivars through conventional breeding approaches due to its long juvenility and some unique reproductive biological features such as gamete sterility, nucellar embryony, and high level of heterozygosity. Due to global warming and changes in consumer trends, establishing a systematic and efficient breeding programs is highly required for sustainable production of high quality fruits and diversification of cultivars. Recently, reference genome sequences of sweet orange and clementine mandarin have been released. Based on the reference whole-genome sequences, comparative genomics, reference-guided resequencing, and genotyping-by-sequencing for various citrus cultivars and crosses could be performed for the advance of functional genomics and development of traits-related molecular markers. In addition, a full understanding of gene function and gene co-expression networks can be provided through combined analysis of various transcriptome data. Analytic information on whole-genome and transcriptome will provide massive data on polymorphic molecular markers such as SNP, INDEL, and SSR, suggesting that it is possible to construct integrated maps and high-density genetic maps as well as physical maps. In the near future, integrated maps will be useful for map-based precise cloning of genes that are specific to citrus with major agronomic traits to facilitate rapid and efficient marker-assisted selection.

• Food Science and Preservation
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• v.13 no.2
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• pp.125-130
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• 2006

In order to study the storage stability of winter date (Zizyphus jujuba Miller) with functional MA film storage, linear low density polyethylene $(LLDPE,\;30{\mu}m\;and\;60{\mu}m)$ film packaging and non-packaging were compared $30{\mu}m$ LLDPE packaging, $60{\mu}m$ LLDPE packaging and non-packaging samples were stored at $0^{\circ}C,\;8^{\circ}C$ (low temperature) and $25^{\circ}C$(room temperature) for 10 weeks. Weight loss of $30{\mu}m$ LLDPE packaging, $60{\mu}m$ LLDPE packaging and non-packaging in room temperature were decreased by 3.6% 0.4% and 36.1% respectively. Titratable acidity showed the trend of similarity in all treatment during the storage conditions, and especially LLDPE film packaging showed higher than non-packaging in the last storage. Soluble solids showed an increase in non-packaging, a decrease in LDPE film packaging highly. Vitamin C content showed the trend of decrease in all treatments, but non-packed jujubes sustained higher than LLDPE film packaging.