Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Production of the Functional Egg to Strengthen Isoflavone : Improvement of Transfer Efficiency of Genistein into the Egg Yolk

Isoflavone 강화 기능성 계란 생산 : 난황내 Genistein 전이 효율 개선

  • HwangBo, J. (National Livestock Research institute, RDA) ;
  • Lee, B.S. (National Livestock Research institute, RDA) ;
  • Lee, H.J. (National Livestock Research institute, RDA) ;
  • Chung, W.T. (National Livestock Research institute, RDA) ;
  • Cho, S.B. (National Livestock Research institute, RDA) ;
  • Hong, E.C. (National Livestock Research institute, RDA) ;
  • Bae, H.D. (National Livestock Research institute, RDA) ;
  • Chang, J.S. (Department of Agricultural Science, Korean National Open University)
  • Published : 2006.09.01
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Abstract

This study was carried out to determine whether genistein implants in laying hens could be transferred into their eggs. 250 mg genistein pellet was implanted two or four subcutaneously in the neck of laying hens. The contents of genistein in egg yolk transferred were analyzed with HPLC-MS. In 500 mg genistein pellets, it was detected as 395 ng/egg yolk on the day 18 after implanting and maintained as 546 ng/egg yolk after the day 59. In 1,000 mg, genistein was detected as 240 ng/egg yolk on the day 13, as 514 ng/egg yolk on the day 30 and maintained over 59 days. In conclusion, the direct genistein implants could be more twenty times efficiently transferred to egg yolks than dietary supplement.

Genistein의 전이율을 조사하기 위하여 순도 97% 이상의 genistein 250 mg을 펠렛으로 성형한 후 500 mg및 1 g의 용량으로 각각 2, 4개씩 산란계의 어깨 부위 피하에 이식하여 계란의 난황 내에서 검출되는 genistein의 함량을 HPLC 및 HPLC-MS를 이용하여 분석하였다. Genistein 500 mg 처리구에서, 12, 18, 30일에 각각 218, 395 ng/g, 500 ng/g의 genistein 이 전이되어 59일까지 계속적으로 유지되었다. 또한 genistein 1,000 mg 처리구에서는 12일 후에는 240 ng/g, 18일 후에는 406.5 ng/g, 30일 후에는 514 ng/g의 genistein이 전이되어 59일까지 계속적으로 유지되었다. 본 연구를 통하여, 500 ng/g 이상의 genistein이 함유된 계란을 59일 이상 지속적으로 생산할 수 있어 피하이식을 통한 비급이 방식은 기존의 급이 방식에 비해 20배 이상 효과적이었다.

Keywords

References

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