• Journal of Life Science
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• v.19 no.6
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• pp.728-734
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• 2009

In this work, a simple, inexpensive and reproducible technique of flotation density gradient centrifugation was developed to isolate monocytes with high purity and yield from peripheral blood mononuclear cells (PBMC) using Histopaque solution, density and osmolarity of which were modified to 1.072 g/ml and 335 mOsm with phosphate-buffered saline (PBS) and sodium chloride (NaCl) solution, respectively. The average purity of monocytes was 74.75${\pm}$3.84%, with the individual purity ranging from 71.44% to 82.38%. The average yield of monocytes was 32.62${\pm}$11.16%, with the individual yields ranging from 21.02 to 53.63%. The monocytes isolated by floatation density gradient centrifugation could be successfully cultured into morphologically, phenotypically and functionally dendritic cells in vitro. In conclusion, the entire procedure seemed to be faster and more convenient, simple and cost-effective than other monocyte isolation methods, including plastic adherence and density gradient methods, and has the potential to be developed as a closed system for clinical scale generation of dendritic cells.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.146-146
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• 1993

신장에서 브라디키닌(Bradikinin, BK)의 생리적 역할을 규명하기 위한 첫 단계로 토끼신장 근위세뇨관의 일차배양세포를 이용, BK 수용체를 ($^3$H) BK를 이용하여 수용체결합실험을 함과 아울러 세포배양과정에서 여러 성장인자들의 BK 수용체발현에 미치는 영향을 관찰하였다. 첫째, 토끼 신장의 피질, 수질 및 근 위세뇨관 둥 각 부위에 대한 BK 수용체결합 실험결과 신수질 부분에 가장 많은 BK 수용체가 발견되었으며 이때 해리항수는 0.52 nM, 그리고 최대결합부위는 mg 단배질당 112.5 fmol이었다. 둘째, serum free 배지에서 insulin, transferrin 그리고 hydrocortisone이 성장인자로 사용될 때 BK 수용체발현이 가장 높았으며 이 중 인슐린에 가장 큰 영향을 받았다. 한편 인슐린의 최적농도를 결정하는 실험의 결과 5 $\mu\textrm{g}$/ml매서 가장 높은 수용체발현을 나타내었으며 그 이상에서는 별다른 차이를 보이지 못했다. 세째, 위의 세 성장인자에 prostaglandin E$_1$이나 triiodothyronine을 첨가시 BK 수용체발현은 오히려 저하되었다. 네째, fetal bovine serum (FBS)과 위의 세 성장인자간의 수용체발현능을 비교한 실험에서 세포배양 후 첫 일주일에는 FBS가 세 성장인자보다 약간 나은 수용체발현능을 나타내었으나 그후 이주째에는 세 성장인자가 BK 수용체발현에 더 적절한 요소임을 보여주었다. 이상의 결과로 보아 토끼 근위세뇨관 상피세포에서의 BK 수용체발현은 세포성장인자로 insulin, transferrin, hydrocortisone 중 insulin에 가장 큰 영향을 받는것으로 보이며, 이들 세가지 성장인자는 serum free 배지에서 세포성장 및 기능에 많은 영향을 주는 것으로 생각된다.prolidine이 $K_{M}$ /K$_{H}$ 비가 가장 높았고 diphenidol이 가장 낮았다. 이상의 결과로 보아 항 histamine제의 muscarinic receptor 차단작용은 이들 약물의 항 alleragy 효과에 필요한 작용이 아니며 본 실험에서 추정된 항 histamine제의 H$_1$-receptor와 muscarinic receptor에 대한 상대적 역가는 이들 약물의 선택과 평가에 중요한 지표가 될수 있을 것으로 생각된다.ing ischemic insults. The nature of the receptor is being explored by molecular genetic techniques, and we have recently cloned two of the major subunits; some of the data will be presented.LIFO, 우선 순위 방식등을 선택할 수 있도록 확장하였다. SIMPLE는 자료구조 및 프로그램이 공개되어 있으므로 프로그래머가 원하는 기능을 쉽게 추가할 수 있는 장점도 있다. 아울러 SMPLE에서 새로이 추가된 자료구조와 함수 및 설비제어 방식등을 활용하여 실제 중형급 시스템에 대한 시뮬레이션 구현과 시스템 분석의 예를 보인다._3$", chain segment, with the activation energy of carriers from the shallow trap with 0.4[eV], in he amorphous regions.의 증발산율은 우기의 기상자료를 이용하여 구한 결과 0.05 - 0.10 mm/hr 의 범위로서 이로 인한 강우손실량은 큰 의미가 없음을 알았다.재발이 나타난 3례의 환자를 제외한 9례 (75%)에서는 현재까지 재발소견을 보이지 않고 있다. 이러한 결과는 다른 보고자들과 유사한 결과를

• Journal of Animal Science and Technology
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• v.48 no.5
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• pp.651-662
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• 2006

Phthalate esters that are used as plasticizers and also found at low levels in foods such as dairy products are often mentioned as suspected endocrine disrupters. The purpose of the present study is to elucidate whether perinatal exposure to di-n-butyl phthalate(DBP), diisononyl phthalate (DINP) and di-2-ethylhexyl adipate (DEHA) affects several aspects of reproductive function in rats especially sexual differentiation of the brain. To this end, the dams were provided with pulverized soy-free diet containing 20, 200, 2,000 and 10,000 ppm of DBP, 40, 400, 4,000 and 20,000 ppm of DINP, or 480, 2,400 and 12,000 ppm of DEHA from gestational day (GD) 15 to postnatal day (PDN) 21, the day of weaning, and serum sex steroid hormone, gonadotropin levels and sexual behaviors after maturation were assessed. At Postnatal week (PNW) 20-21, serum levels of sex steroids and gonadotropins in both male and female rats, as well as estrous cyclicity in females, were not changed by perinatal exposure to DBP, DINP and DEHA, indicating that these chemicals did not affect sexual differentiation of the brain controlling the endocrine system of hypothalamo-pituitary-gonadal (HPG) axis. On the other hand, inhibitory influences on sexual behaviors, especially on ejaculation in males and lordosis in females, were observed by perinatal exposure to these chemicals. These results suggest that these chemicals may act directly on discrete regions of the hypothalamus regulating sexual behaviors, but not regulating gonadotropin secretion, thereby affect sexual differentiation of the brain with a resultant decrease in sex-specific behaviors in adulthood.

• Korean Journal of Food Science and Technology
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• v.40 no.1
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• pp.21-30
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• 2008

In this study, the antioxidant activities of 27 medicinal herbs and plants were measured. The dried medicinal herbs and plants were pulverized and extracted by water, 15% ethanol, and 45% ethanol, respectively at above $80^{circ}C$ for 90 minutes. The original and diluted extracts were tested for their antioxidant activities by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging activity assay. Cornus officinalis (C) and Scutellaria baicalensis (S) were selected to develop rice wines with higher antioxidant activities, based on the result of the 45% ethanol extraction. The rice wine developed using sample showed the highest antioxidant activity as comparing to those of other commercial rice wines. The rice wine samples were analyzed for titratable acidity, pH, $^{\circ}Brix$, reducing sugar content, color (L, a, b), amino-acidity, and ultraviolet absorption. The preferences for color, aroma, and overall acceptability were determined using a 9-point hedonic scale by 150 consumers. The sweetness, sourness, fruitiness, color, and medicinal herb-taste levels of the developed rice wines were also evaluated, using a 9-point just-about-right scale. The mean overall acceptability score of c (5.54) was higher than that of sample S. Based on the results, the sourness and medicinal herb-taste levels of sample s should be modified to higher levels, and the fruitiness of sample S also needs to be adjusted. For a future study, the final compositions of the developed rice wines will be adjusted for product launching based on the preference test data acquired in this study.

• Journal of Information Management
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• v.38 no.2
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• pp.153-173
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• 2007

[ $OntoFrame^{(R)}$ ] system provides information dissemination service and inference service, based on Semantic Web framework to fully support R&D activities. Although it is one of state-of-the-art systems in the viewpoint of functionality, we are not able to declare whether it has satisfiable usability because of the omission of usability test in development process. Thus, this research tries to reveal the usability level of the $OntoFrame^{(R)}$, and further to find ways to achieve a user-center system. Both 'theory-based assessment' by a software ergonomics expert and 'user test' by four users are used for evaluating the usability of the $OntoFrame^{(R)}$. We look forward this research to being a basic reference for practical systems aiming at satisfiable usability.

• Proceedings of the Korean Association of Geographic Inforamtion Studies Conference
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• 2004.03a
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• pp.599-605
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• 2004

기존의 구축된 GIS 컴포넌트 혹은 개발 중이거나 향후개발을 목표로 설계단계에 있는 컴포넌트들의 최종 목표는 재사용성과 상호운용성의 가능성 여부이다. 하지만 컴포넌트 개발에 있어 시스템 개발환경의 다양성으로 인하여 그 재활용성은 생각만큼 쉬운 작업이 아니며, 특히 공간정보를 다루고 있는 GIS(Geographic Information System)분야에서의 GIS 컴포넌트 재활용은 전 세계의 산재한 각 데이터형의 포맷, 개발 환경, 운영환경을 고려하여 볼 때 시급한 일임에도 불구하고 그에 대한 노력이 상당히 미진한 실 정 이 다. 본 논문에서는 GIS 애플리케이션을 보다 효율적이고 유용하게 개발하기 위하여 GIS 컴포넌트의 개발과 관리에 이르는 전 과정을 관리 감독할 수 있는 COGIS(Component Oriented Geographic Information System)을 제안하고, COGIS 프로세스의 가이드라인이며 GIS 컴포넌트의 기능적인 면을 정의하기 위한 GCA(GIS based Component Architecture) 아키텍처를 제안하였다. 아울러 GIS 컴포넌트의 메타데이터를 분류 및 정의하여 GIS 컴포넌트의 비 기능적면을 제시하고 이를 이용하여 웹 기반 GIS 컴포넌트 등록/검색 에이전트 시스템을 개발하였으며 기존 GIS 컴포넌트 재사용 및 확장, 신규 컴포넌트의 등록, 검색이 가능하도록 한다. 사례연구로 웹 상에서 산불 발생 위험지수 표출을 위한 GIS 공간 분포도 작성이 쉽게 이루어지도록 2FDRV.avx와 2FDRC.exe 컴포넌트를 개발하였으며, COGIS 프로세스의 컴포넌트 관리방법을 통하여 여러 관련 컴포넌트를 조합함으로써 웹 기반 산불위험지수예보시스템을 구축하였다.입력 근거의 확보’, ‘갱신주체별 역할의 정의 및 유지관리 기준의 설정’, ‘분야별업무 특성을 고려한 관련 기준의 마련 및 타 시스템과 연계되는 항목을 고려한 절차 정의’ 등에 대한 다양한 접근을 시도하였다. 본 연구에서 제시하는 유지관리 모델을 기반으로 각 지자체별로 적절한 컨설팅이 진행되고 이에 따라 담당자의 실천이 이루어진다면 지자체 GIS의 투자대비 효과에 대한 기대는 이상이 아닌 현실로 다가오게 될 것이다.가오게 될 것이다. 동일하게 25%의 소유권을 가지고 있다. ？스굴 시추사업은 2008년까지 수행될 계획이며, 시추작업은 2005년까지 완료될 계획이다. 연구 진행과 관련하여, 공동연구의 명분을 높이고 분석의 효율성을 높이기 위해서 시료채취 및 기초자료 획득은 4개국의 연구원이 모여 공동으로 수행한 후의 결과물을 서로 공유하고, 자세한 전문분야 연구는 각 국의 대표기관이 독립적으로 수행하는 방식을 택하였다 ？스굴에 대한 제1차 시추작업은 2004년 3월 말에 실시하였다. 시추작업 결과, 약 80m의 시추 코아가 성공적으로 회수되어 현재 러시아 이르쿠츠크 지구화학연구소에 보관중이다. 이 시추코아는 2004년 8월 중순경에 4개국 연구팀원들에 의해 공동으로 기재된 후에 분할될 계획이다. 분할된 시료는 국내로 운반되어 다양한 전문분야별 연구에 이용될 것이다. 한편, 제2차 시추작업은 2004년 12월에서 2005년 2월 사이에 실시될 계획이다. 수백만년에 이르는 장기간에 걸쳐 지구환경변화 기록이 보존되어 있는 ？스굴호에 대한 시추사업은 후기 신생대 동안 유라시아 대륙 중부에서 일어난 지구환경 및 기후변화를 이해함과 동시에 이러한 변화가 육상생태계 및 지표지질환경에 미친 영향을 이해하는데 크게 기여할 것이다.

• Convergence Security Journal
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• v.5 no.4
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• pp.41-48
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• 2005

Objective of this study is to design and implement security system based on intrusion tolerance technology for the improvement of dependability in defense system. In order to do so, I identify and extract core technologies through the research and analysis into characteristics, structures, main functions, and technologies of intrusion tolerance architecture. And I accomplish a design of security system through the redundant system based on these core technologies. To implement and verify intrusion tolerance system, I chose 'wargame system' as a subjected system, and accomplished 'Wargame Intrusion Tolerance System' and verified security required functions through a performance test. By applying showed security system into the development of application software based on intrusion tolerance, systematic and efficient system could be developed. Also applying 'WITDS' can solve the current security problems, and this will be basic model for design of security architecture in the federation system after.

• Journal of Life Science
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• v.23 no.2
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• pp.157-166
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• 2013

This study aimed to investigate whether nociceptin contributes to the alterations in cerebral blood flow (CBF) regulation following postnatal exposure to ethanol in Sprague-Dawley rats. Animals received ethanol twice a day, 2 hr apart, on postnatal 6, 7 and 8 days. The changes in regional CBF (rCBF) in response to the changes in mean arterial blood pressure were determined at 4-, 8-, and 12-week of age by laser-Doppler flowmetry. Hypotension was induced by the gradual withdrawal of blood from arterial catheter, and the reversal of blood pressure was produced by the reinfusion of blood. Expression of nociceptin-like immunoreactivity was determined in dura mater and cerebral cortex using immunohistochemistry. Postnatal exposure to ethanol almost abolished the autoregulation of rCBF in all age groups. Pretreatment with nociceptin but not with [$Nphe^1$]nociceptin(1-13)$NH_2$, a selective competitive nociceptin receptor antagonist, 5 min prior to ethanol administration preserved the autoregulation of rCBF in all age groups. Postnatal exposure to ethanol markedly increased the expressions of nociceptin-like immunoreactivity in the dura mater and cerebral cortex, both of which were significantly inhibited by pretreatment with 7-nitroindazole monosodium salt as well as aminoguanidine 5 min prior to ethanol administration in all age groups. The values of arterial blood gas analysis were not significantly different from the basal levels in all groups. These results suggest that nociceptin deeply contributes to the compensatory mechanisms for the nitric oxide-dependent alterations in CBF autoregulation following postnatal exposure to ethanol.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.4
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• pp.669-676
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• 2017

Recently, due to the importance of information security, security vulnerability analysis and various information protection technologies and security systems are being introduced as a countermeasure against cyber-attacks in new as well as existing buildings, and information security studies on high-rise buildings are also being conducted. However, security system introduction and research are generally performed from the viewpoint of general IT systems and security policies, so there is little consideration of the infrastructure of the building. In particular, the BAS or building infrastructure, is a closed system, unlike typical IT systems, but has unique structural features that accommodate open functions. Insufficient understanding of these system structures and functions when establishing a building security policy makes the information security policies for the BAS vulnerable and increases the likelihood that all of the components of the building will be exposed to malicious cyber-attacks via the BAS. In this paper, we propose an architecture reference model that integrates three different levels of BAS structure (from?) different vendors. The architectures derived from this study and the security characteristics and vulnerabilities at each level will contribute to the establishment of security policies that reflect the characteristics of the BAS and the improvement of the safety management of buildings.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.11
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• pp.18-23
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• 2018

FPSO consists of topside modularized plants for production of crude oil, and hullside structures that serve as support for the topside and storage of produced crude oil. The structural behavior of the FPSO topside module and its supporting hull depends on the interface structure that connects them, and the interface structure consists of a combination of individual unit support structures called Module Support Seat (MSS). Types of interface structures are various and, accordingly, the basic design of the FPSO topside module structure is greatly influenced, so various design methods should be considered from the initial design phase. Structural design of FPSO topside module requires consideration of the number of MSSs, connection type, and structural analysis options such as the range of finite element models, load conditions, and boundary conditions for verification of structural strength. In this study, the comparison combination cases for the above considerations were derived and the strength evaluation was performed, and the structural behavior characteristics of the topside module were compared and analyzed through a detailed review of the analysis results. The results of this study are considered to be a good reference for designing a more reliable topside module structure.