• Journal of the Korean Society of Food Science and Nutrition
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• v.15 no.1
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• pp.56-62
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• 1986

Marine fishes, sardine(Sardinops melanosticta), scad kingfish(Caranx equula), horse mackerel(Trachurus japonicus) and file fish(Navodon modestus), were stored for fifty days with partial freezing at$-3.5^{\circ}C$. During the storage, the changes in microflora and volatile nitrogen content was investigated. The fishes exhibited $10^4\;to\;10^6$ of bacterial cells per square centimeter of their skin just before they were submitted to the storage. The bacterial cell number was increased as $10^6\;to\;10^8$ cells as the storage time passed over twenty-two days. Offensive odor which is typical in the spoilage of fishes became strong as increase the bacterial cell numbe. The major isolates among the three hundred strains of bacteria isolated from the fish skins were identified as Pseudomonas I/II, III/IV-H, Vibrio and Moraxella. The same was found in the spoiled fishes, however, Pseudomonas I/II, was predominant on contrast to that of fresh fishes. Pseudomonas III/IV-NH, Flavobacterium, Cytophaga and Micrococcus were also found in early period of storage, but they disappeared as the progress of storage. Nine per cent of isolates were unidentified.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.163-163
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• 1993

대상환자는 모두 23명이였으여, 이중 12명은 fluconazole을, 11명은 nystatin을 투여받았다. 두 군사이에 연령, 남여비, 기저질환, 진균감염의 위험요소는 차이가 없었다. 투약기간은 fluconazole군(F)이 24일, nystatin군(N)이 23일이였다. 중도탈락은 3례로 F중 MRSA ？혈증 1례, N중 오심, 구토 1례외 Stevens-Johnson증후군 1례였다. 평가가능한 20례중, F 11례중 8례와, N 9례중 4례에서 진균감염중의 예방에 성공하였고, F 3례와 N 5례는 실패하였다. 실패한 F 3례는 코의 피부조직에서 aspergillus가 중명된 1례와 경험적으로 amphotericin을 투여한 2례였고, N 5례는 혈액배양에서 Candida tropicalis가 분리된 1례와 경험적 amphotericin을 받은 4례였다. 진균의 colonization은 F중 3균주는 치료중 소실되었으나, 2례는 증가하였다. N은 1균주가 소실된 반면, 3례는 증가하였다. Colonization이 증가한 F 2례는 C. albicans와 Trichosporon beigelli가 대변에서 분리된 경우이고. M 3례는 대변에서 C. albicans가 분리된 1례, 구강에서 aspergillus가 분리된 1례, 혈액배양에서 C. tropicalis가 분해된 1례였다. 부작용은 오심 구토가 F중 1례, N중 2례 였으며, 이중 N의 1례는 증상이 심하여 투약을 중지하였다. 간기능 검사상 SGOT/SGPT의 상승은 F 12중 3례, N 11중 2례에서 관찰되었으나, 두 군사이에 통계학적으로 유의한 차이는 없었다.

• Membrane Journal
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• v.31 no.4
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• pp.282-292
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• 2021

In this work, we tested commercial organic solvent nanofiltration (OSN) membranes using both in-house dead-end and crossflow systems. Four different crosslinked polyimide Duramem (DM) OSN membranes with various MWCO (molecular weight cut off) values were tested in organic solvents such as ethanol, N,N-dimethylformamide, acetone and acetonitrile. The membranes exhibited more reliable and reproducible performance in the crossflow system, and the performance changed significantly depending in the physical properties of the testing solvent. This is due to the initial stabilization period via pressure-induced compaction phenomenon, which can be vastly different between membrane samples. Hence, to obtain reliable and reproducible results, crossflow system is the preferred choice.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.3
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• pp.357-363
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• 2000

고추장의 품질을 향상시키고 재래식 전통 고추장과 유사한 맛을 지닌 고추장을 만들기 위해 재래식 전통 고추장에서 분리한 B.licheniformis 균주와 공장에서 대량 생산을 위해 개량해온 A. oryzae와, 비교적 호염성이며 알콜 발효 능력이 우수한 S.rouxii를 혼용하여 고추장을 담근 후 180일까지의 각 고추장의 이화학적 특성 변화를 조사한 결과는 다음과 같다. 단도는 모든 구에서 숙성 30일 까지 급격히 증가한 후 180일까지 거의 일정한 수준을 유지하였으며, 곰팡이 단용구에 비해 고초균과 효모 혼용구에서 숙성 기간 동안 높은 적정산도 값을 보였다. 조단백질은 숙성 기간 동안 대조구에서는 숙성 60일까지 약간 증가한 후 숙성 120일까지 급격히 감소하였으며 고초균 혼용구와 효모 첨가구는 숙성 90일 이후 감소한 후 숙성 120일 이후부터는 거의 변화가 없었다. 숙성 90일 이후부터는 B. licheniformis와 효모 혼용구에서 A. oryzae 단용구보다 조단백질 함량이 높았다. 환원당은 세 구간 모두 숙성 60일 경까지는 증가하는 추세를 보였으며, A. oryzae 단용구보다 B.licheniformis 균주와의 혼용구에서 높은 수치를 보여주었다. 비휘발성 유기산은 숙성 기간이 경과하면서 증가하였고 가장 많이 검출된 유기산은 pyroglutamic acid와 citric acid의 순이었다. 따라서 고추장의 숙성 과정 중 이화학적 특성 변화의 측면에서 볼 때 고초균과 효모 혼용구로 제조된 고추장이 구수한 맛의 생성면에서 유용한 담금법인 것으로 사료된다.

• Journal of Korea Water Resources Association
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• v.32 no.3
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• pp.247-254
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• 1999

The frequency analyses of annual maximum rainfall data for 22 rainfall gauging stations is Korea were performed. The method of moments (MOM), maximum likelihood (ML), and probability weighted moments (PWM) were used in parameter estimation. The GEV distribution was selected as an appropriate model for annual maximum rainfall data based on parameter validity condition, graphical analysis, separation effect, and goodness of fit tests. For the selected GEV model, spatial analysis was performed and rainfall intensity-duration-frequency equation was derived by using linearization technique. The derived rainfall intensity-duration-frequency equation can be used for estimating rainfall quantiles of the selected stations with convenience and reliability in practice.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.194.2-195
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• 2003

본 연구는 수출방법의 개선과 절임 가공식품의 개발하고자 일본 수출용으로 재배하고 있는 축양종의 절임가지의 저장방법과 저장 중에 당류, 유기산 및 아미노산의 변화를 분석하였다. 총당 함량과 환원당 함량은 저장방법에 관계없이 저장기간이 길어질수록 조금씩 감소하였고, 침지 저장한 것보다는 진공포장하여 저장한 것이 변화가 적었다. 가지에서 분리 정량된 유기산은 acetic acid, citric acid, lactic acid, malic acid 그리고 succinic acid 총 5종이었으며 acetic acid와 malic acid의 함량이 가장 높았으며, 저장기간이 길어질수록 저장 초기에 비해 acetic acid의 함량은 증가하였는데 반해 malic acid의 함량은 감소하였다. 가지의 주요한 구성아미노산은 valine, aspartic acid, glutamic acid, glycine, alanine 등이었으며, 총아미노산 함량은 진공포장하여 저장한 경우에는 저장 20 일째 1488.14mg/100g으로 생가지보다도 증가하였는데 반해 침지하여 저장한 경우에는 총 아미노산 함량이 745.42mg/100g으로 서 저장 초기에 비해 감소함을 나타내었다. 유리아미노산은 aspartic acid, alanine, cystine과 proline 등의 함량이 높았고, 저장 기간이 길어질수록 저장초기에 비해 유리아미노산의 함량이 증가하였는데, 특히 진공 포장하여 저장한 가지에 비해 침지 저장 한 가지에서 그 함량이 높았다. 또한 아미노산 유도체의 함량은 phosphoserine, taurine, ${\gamma}$-aminoisobutyric acid 및 hydroxyproline의 함량이 높았고, 저장중에 phosphoserine의 함량은 감소하였는데 반해 ${\gamma}$-aminoisobutyric acid는 증가하였다. 축양품종의 절임가지의 저장방법과 저장기간에 따른 성분의 변화를 고려할 때 진공포장하는 것이 절임가지의 품질 유지할 수 있는 것으로 생각된다.

• Korean Journal of Food Science and Technology
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• v.23 no.4
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• pp.452-458
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• 1991

Psychrotrophs producing protease were isolated during ripening periods of Cheddar cheese and one of them containing the highest protease activity was identified as Pseudomonas fluorescens 65. The extracelluar proteolytic enzyme was partially purified from P. fluorescens 65 through the Sephadex G-100 gel filtration. The protease was eluted between 190 ml and 230 ml of elution volume of sodium phosphate buffer. The purified protease showed a single band in SDS-PAGE and its molecular weight was 47,000. The composition of amino acid for the protease was determined and the most abundant amino acids were glutamic acid (14.96%) and serine (13.86%). The optimum temperature and pH for the activity was $45{\sim}50^{\circ}C$ and 6.0, respectively.

• Journal of Korea Water Resources Association
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• v.37 no.5
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• pp.397-406
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• 2004

It is necessary to estimate the groundwater recharge rate properly to evaluate the reasonable development amount of groundwater in a specific site. A small basin in Wicheon River Basin located in the Province of Kyungsangbuk-Do is selected to calculate the groundwater recharge rate. Average annual groundwater recharge rates are calculated from 1992 to 1997 because wet and draught year are contained during this period. In the calculation, baseflow separation method and SCS-CN method are applied to this area. As a result of estimation by baseflow separation method, the value of groundwater recharge ratio is varied between 11.9% and 18.7%. The average annual recharge rate is 14.5%. The average annual recharge rate calculated by SCS-CN method is varied between 7.9% and 20.9%. The average annual recharge rate in the calculation period is 15.1%. The results show that the average annual recharge amount from infiltration in the study basin is 141.6mm and 147.4mm in each estimation method. It appears that the average annual recharge amount calculated for the long period containing wet and draught year by the two methods is useful for groundwater development.

• Korean Journal of Microbiology
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• v.46 no.4
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• pp.346-351
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• 2010

The purpose of this work was to investigate the characteristics of a biosurfactant-producing bacterium isolated from crude-oil contaminated soils. During the incubation of strain HK-3 with 1% crude-oil, bacterial growth pattern, the amount of biosurfactant production, and pH changes were monitored. In order to examine the effect of supplemented carbons on the production of biosurfactant, cultivation of HK-3 cells in BH media with different carbons (e.g. glucose, dextrose, mannitol, citrate, or acetate) revealed that the production of biosurfactant reached the maximal level at the 72 h incubation with mannitol, which the area of clear zone was measured to approximately 7.64 $cm^2$. Identification test using the BIOLOG system, morphology study based on scanning electron microscopy and the 16S rRNA sequence-based phylogenetic analysis assigned strain HK-3 to a Pseudoalteromonas species, designated as Pseudoalteromonas sp. HK-3 which was registered in GenBank as [FJ477041].

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.4
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• pp.549-555
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• 1997

Twenty species of lactic acid bacteria were isolated from Kimchi. Six species were identified as Lactobacillus sake, ten species as Leuconostoc mesenteroides and four species as Lactobacillus plantarum. The ability of these isolates to deplete nitrite during growth at 15, 20, 25 and 3$0^{\circ}C$ in MRS broth containing 250$\mu\textrm{g}$/$m\ell$ of nitrite was examined. Depletion of nitrite was high as the order of L. plantarum, L. sake and L. mesenteroides at all temperature tested, and the depletion activity was increased as the increase of growth temperatures. Especially, almost all of nitrites were utilized by L. plantarum during growth at all temperature ranges tested. L. plantarum and L. sake required induction periods for adapting to nitrite, but L. plantarum was remarkably depleted nitrite after two days of growth at 15$^{\circ}C$ and one day at 2$0^{\circ}C$, L. sake after one day of growth at both temperatures. Whereas, L. mesentero-ides did not require those periods at all temperature ranges tested.