• The Korean Journal of Nuclear Medicine
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• v.32 no.4
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• pp.374-381
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• 1998

Purpose: I-131 labeled (2'-deoxy-2'-iodo-${\beta}$-D-arabinofuranosyl) adenine (IAD) may be involved in DNA synthesis during active proliferation of tumor cells. We conducted this study to find out the biodistribution of IAD and it's feasibility for scintigraphic tumor imaging. Materials and Methods: Tosyl acetyl-adenosine was dissolved in acetonitrile, and I-131-NaI was added and heated to synthesize IAD. Female Fisher 344 rats innoculated with breast tumor cells were injected with 0.27 MBq of IAD. Rats were sacrificed at 0.5, 1, 2, 4, 24h and the % of injected dose per gram of tissue (%ID/g) was determined. For scintigraphy, rats bearing breast cancer were administered with 1.11 MBq of IAD and imaging was performed after 2 and 24h. Then, rat body was fixed and microtomized slice was placed on radiographic film for autoradiography. Results: %ID/g of tumor was 0.74 (0.5h),0.73 (1h), 0.55 (2h), 0.38 (4h), and 0.05 (24h), respectively. At 1h after injection, %ID/g of tumor was higher than that of heart (0.34), liver (0.42), spleen (0.47), kidney (0.69), muscle (0.14), bone (0.33) and intestine (0.51). However, %ID/g of tumor was lower than blood (1.06), lung (0.77), and thyroid (177.71). At 4h, %ID/g of tumor in comparison with other tissue did not change. Tumor contrast expressed by tumor to blood ratio was 0.69 and tumor to muscle ratio was 5.11 at 1h. However, these ratios did not improve through 24h. On autoradiogram and scintigraphy at 2 and 24 hour, the tumor was well visualized. Conclusion: This results suggest that IAD may have a potential for tumor scintigraphy. However, further work is needed to improve localization in tumor tissue.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.293-293
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• 2004

본 실험은 도축전 암소에 성선자극호르몬(Gonadon, 동방(주) : 3 ㎖ 목근육 주사, Gonadorelin 100 ㎍/㎖)을 처리하여 인위적으로 새로운 발정주기를 유도하여 난자 회수율 및 수정란 생산성을 향상시키고자 실시하였다. ① 실험대조군으로 85두(T1), 호르몬처리 37두를 대상으로 처리시간(T2 ; 72hrs, T3 ; 96 hrs, T4 ; 120. T5 ; 148 hrs.)에 따라 도축 후 난소로부터 혈통수정란 생산을 위해 개체별로 난자 회수량을 확인하였다. 회수된 난자를 이용하여 체외성숙 및 체외수정을 실시하였고, 이들 난자의 발육상태, 난발달 및 동결가능 수정란수를 각각 확인하였다. (중략)

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.284-284
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• 2004

본 연구는 Elk 사슴에서 Magic Doctor(한경게놈텍)를 이용하여 발정동기화 인공수정 후 재발정 행동의 발현양상을 관찰하기 위해 실시하였다. 미경산록 7두와 경산록 13두를 공시하였고, 발종동기화 및 인공수정은 번식계절인 9월에 progesterone이 3 g 함유된 controlled intravaginal drug-releasing device(CIDR)를 14일간 질내에 삽입하고 제거한 후에 pregnant mare serum gonadotropin(PMSG) 250IU틀 근육 주사하여 발정동기화와 배란을 유도하였다. 인공수정은 CIDR제거 후 60∼62시간째에 실시하였고 인공수정 후 비임신 개체의 재발정 발현 양상을 관찰하였다. (중략)

• Journal of Embryo Transfer
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• v.6 no.1
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• pp.46-55
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• 1991

본연구의 목적은 흰쥐의 임신 초기에 있어서 착상의 유발에 PAF의 관련여부를 PAF의 수용체 길항제인 BN-52021d의 작용과 비교하여 결정하기 위함이다. 임신초기 각일에 점증하는 용량의 BN-52021 (체중 200g당 10$\mu$g 내지 1.25mg)이 근육내로 주사되었을때 10$\mu$g내지 250$\mu$g 용량에서는 대조구에 비하여 착상부위의 수 혹은 이것을 가진 흰쥐의 수에 대하여 현저한 영향을 미치지 아니하였으나 1.25mg투여 경우에는 현저히 감소된 효과를 나타내었다.(P<0.05). PAF(1$\mu$g) 경우에는 대조구와 비교하여 현저한 차이는 없었다. 임신 초기 각일에 BN-52021(1.25mg)과 검증하는 용량의 PAF(0.04$\mu$g 내지 1.0$\mu$g)가 동시에 투여된 경우에는 PAF의 농도가 증가함에 따라서 착상 부위의 수도 증가하는 경향이었으나 현저하지는 아니하였다. 본 실험 결과는 PAF는 흰쥐의 임신 초기 반응에 관련된다는 사실을 보여준다.

• The Korean Journal of Zoology
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• v.35 no.4
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• pp.414-427
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• 1992

Octopus variabilis의 흡반을 광학현미경과 주사전자현미경 그리고 투과전자현미경을 이용하여 그 미세구조를 관찰하였기에 이에 보고하는 바이다. 또한 내측상피 부위 돌기구 조물 속에서 관찰된 세포들은 핵을 둘러싼 세포들이 공포화 현상으로 인하여 해면처럼 보였다. 이 세포들은 감각과 수축에 관여하는 해면질성인 세포로 생각된다. 외측 상피조직에서는 2종의 분비성인 감각세포(A 및 B형세포)차 2종의 상피성인 감각세포(C 및 D형세포)가 관찰되었다. 분비성인 감각세포는 감각이외에 점액분비 기능을 소지하였으며, 상피성인 감각세포는 상피의 지지와 감각기능을 수행하는 것으로 생각되었다. 흠반을 구성하고 있는 근섬유는 중앙이 기린 도나스 형태로서 10-15개의 종선이 나타났다. 또한 부위에 따라서는 근섬유들은 서로 집결하여 원주형의 근육 기등을 형성하기도 했는데 이들은 신경섬유에 의하여 서로 연결 되고 통제되었다.

• Tuberculosis and Respiratory Diseases
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• v.52 no.4
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• pp.317-329
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• 2002

Background : Immunotherapy is another treatment modality for various cancers. There is little information on the antitumor effects of immunotherapy on implanted lung cancer mouse models. Toxoplasma gondii is able to potently induce a nonspecific stimulation of the host immune system. Therefore, this study evaluated the antitumor and antimetastatic effect of nonspecific immune stimulation by T. gondii in a Lewis lung cancer mouse model. Methods : Female C57BL/6 mice were injected with either Lewis lung cancer cells ($1{\times}10^6$ per mouse) or 5 cysts from the T. gondii Me49 strain with various schedules. The number of survival days, the tumor size of the implanted muscle and the histopathological findings of each group were noted. In addition to these mice, the Toxoplasma antigen($50{\mu}g$ per mouse) or a lymphokine (0.5 ml per mouse) was added to boost the immunotherapy. Results : No mouse in the Toxoplasma-infected group had died, whereas the mice receiving only the cancer cells (cancer control) survived for $29.1{\pm}4.4$ days. Cancer cells were revealed from 1 week after cancer cell inceulation in the muscle and from 3 weeks in the lung of the cancer control, whereas cancer cells were found in both the preinfection control and coinfection control groups from 2 weeks and 4 weeks in the lung respectively. The in the number of survival days were $32.4{\pm}3.3$ in the mice receiving T. gondii 2 weeks prior to the cancer cells inoculation (preinfection control), $30.9{\pm}5.1$ in mice received both simultaneously (coinfection control), and $34.9{\pm}2.9$ in mice received T. gondii 2 weeks after cancer cells implantation (postinfection control). These 3 infection groups had significantly longer survival days and suppressed tumor growth than those of the cancer control. In addition to these mice, and injection with the Toxoplasma antigen alone or in combination with lymphokine resulted in a significant increase in the number of survival days. Conclusion : These findings suggest that an injection with T. gondii can induce the antitumor and antimetastatic effects in Lewis lung cancer mouse models. Moreover, these effects were increased with an injection of the Toxoplasma antigen alone or in combination with lymphokine. However, this therapy can not prevent the development of cancer.

• Korean Journal of Medicinal Crop Science
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• v.2 no.3
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• pp.181-186
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• 1994

Vegetative node and rhizome of Ligusticum chuanxiong Hort. were planted in spring and autumn and harvested after one and two years to determine the appropriate harvesting time which produced a high yield. Plant height, leaf number and branch number per plant were increased in order of rhizome planted in spring and harvested after two year(RST), rhizome planted in autumn and harvested after one year(RAO), vegetative node planted in autumn and harvested after one year(VNAO), and vegetative node planted in spring and harvested after one year(VNSO). Leaf area index and dry leaf weight in VNSO were highest on August 16, but dry weights of stem and rhizome was increased until harvesting time. The appropriate harvesting time was October 17, in RST, November 9 in RAO and VNAO, and November 13 in VNSO. Yield in autumn planting was more increased than that in spring planting and also that in RST was 443kg per 10a and increased by 2.8 times compared to RAO. However the yield in the rhizome planting was more increased by 17 percent than the vegetative node planting, the latter planting was inexpensive and economic for purchasing seed materials.

• Korean Journal of Medicinal Crop Science
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• v.2 no.3
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• pp.198-204
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• 1994

This study was carried out to investigate the auxin(IAA, IBA, NAA) treatment and the effect of shading rate in Asarum sieboldii. The results obtained were summerized as follows: By the soaking treatment of auxins to the cutted rhizome enhanced root growth and plant weight. By the increment of shading rate, plant growth was much better compare to the control. Leaf fallen times appeared about 20 days more earlier at plain area then the alpain area. Root yield was much higher by the treatment of shading then the conventional cultivation so it seem to be the useful for large scale cultivations of A. sieboldii.

• Journal of Veterinary Clinics
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• v.30 no.6
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• pp.415-420
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• 2013

This study was performed to examine the anesthetic and cardiopulmonary effects of medetomidine, midazolam and ketamine (MMK) combination in ten beagle dogs. Dogs were randomly allocated to two groups. Treatment group MMK-L received 0.015 mg/kg medetomidine followed by 0.3 mg/kg midazolam and 5 mg/kg ketamine by intramuscular injection. Treatment group MMK-H received 0.02 mg/kg medetomidine followed by 0.3 mg/kg midazolam and 5 mg/kg ketamine by intramuscular injection. Induction, anesthesia, sternal recumbency, standing, walking time, heart rate, arterial blood pressure, rectal temperature, respiratory rate and arterial blood gases were measured. Mean anesthesia time was significantly different between MMK-L group ($52.4{\pm}11.08$ minutes) and MMK-H group ($78.2{\pm}20.72$ minutes). Sedative scores and noxious stimuli were raised to the maximum value at 5 minutes after administration of the test dose and maintained until 40 minutes in both groups. In both groups, the heart rate significantly decreased after MMK administration. The blood pressures (MAP, SAP and DAP) increased after MMK administration but there were no significant differences in blood pressures between two groups. In conclusion, intramuscular administration of medetomidine followed by intramuscular injection of midazolam and ketamine in beagle dogs, leads immediate and sufficient anesthesia and proper doses of medetomidine for minimal adverse effects in intramuscular MMK combination will be 0.015 mg/kg in dogs.

• The Korean Journal of Nuclear Medicine
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• v.32 no.1
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• pp.89-98
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• 1998

The search for tumor-avid agents for use in nuclear medicine imaging is an ongoing field of importance. The purpose of this study was to determine the affinity for radio labeled vitamin $B_{12}$ by a wide histologic variety of tumor types in mice. Seventeen different types of tumor were grown subcutaneously in female Balb/C or Balb nu/nu(nude) mice. When the tumors reached about 1 cm in diameter, mice were injected intraperitoneally with $^{57}Co$-vitamin $B_{12}$. Twenty-four hours later, the mice were sacrificed. Organs and tissues were removed, weighed, and activity per mg determined by gamma counter. Values represented cpm/mg tissue that was normalized to 20 grams body weight for each mouse. A wide variety of tumor types showed significant uptake and concentration of $^{57}Co$-vitamin $B_{12}$, as evidenced by tumor:tissue activity ratios. For many tissues of great importance in terms of background(bone, muscle, blood), the tumor:tissue activity ratios of uptake were high. These data strongly suggest that further efforts to evaluate the utility of radio labeled adducts of vitamin $B_{12}$ for clinical use in oncologic imaging are warranted.