• Animal Systematics, Evolution and Diversity
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• v.13 no.2
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• pp.73-81
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• 1997

한국산 조류 중 박새속(genus Parus)에 속하는 4종 Parus major wladiwostokensis (박새), P. ater amurensis(진박새), P. palustrius hellmayri(쇠박새) 및 P. varius varius(곤줄 박이)를 대상으로 이들의 계통적 유연관계를 구명하기 위하여 mtDNA분석을 실시하였다. 6 base를 인지하는 10개의 제한효소를 처리하여 얻어진 mtDNA의 크기는 16.6~17.0Kb였으며 Pst I과 Pvu II는 종간 차이가 뚜렷하였다. 각 종간의 절편양상을 비교하여 Parus속의 종 간 분화정도를 비교한 결과 P. m. wladiwostokensis와 P. a. amurensis의 유전적 근연관계 가 가장 가까웠고(p=0.073, F=0.294) P. a. amurensis와 P. v. varius는 비교적 현저한 유전 적 차이를 보였다.(p=0.119, F=0.143). Brown 등 (1979)의 공식을 이용하여 박새속 4종의 분 화시기를 추정한 결과 이들은 후기 선신세(Pliocene)와 초기 홍적세(Pleistocene) 사이에 분 화된 것으로 추정되었다.

• The Korean Journal of Malacology
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• v.11 no.2
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• pp.171-179
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• 1995

한국산 다슬기류의 종별 유전적 변이를 알아보고 계통분류학적 근거를 제공하기 위하여 다슬기속(Simisulcospira)에 속하는 다슬기(S. libertina),주름다슬기,(S. forticosta), 곳체다슬기(S. gottschei), 좀주름다슬기(S. tegulata)및 참다슬기(S. coreana)등 5종을 전국 각지에서 채집하여 전기영동(starch gelelectrophoresis)를 이용해 분자유전학적 분석을 실시한 결과를 용약하면 아래와 같다. 유전적 다형형의 빈도는 58%에서 83%로 대단히 높았고 이형접합자의 평균 빈도도 50%내외였다. Rogers' D를 근거로 한 각 종간 유연관계는 주름다슬기와 곳체다슬기가 근연관계를 이루고 있었으며 이들 두 군과 다슬기는 유전적으로 거리를 두고 있었다. 이러한 결과는 패각의 형채와 비교할 때 유사한 경향을 보였다.

• Journal of Environmental Impact Assessment
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• v.29 no.3
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• pp.230-238
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• 2020

This study inferred the ecology of habitat use of the wild boars (Sus scrofa) in Yeongwol Hanbando wetland through DNA analysis using non-invasive samples of hairs. From November 2018 to May 2019, hair samples were collected from rubbing trees and hairtraps within the Hanbando wetland (2.772 ㎢). We extracted DNA from the hair samples and conducted PCR to verify the species and identify sex of the individuals. In addition we analysed 6 microsatellite markers to identify individuals and genetic relationship among the pairs of individuals. A total of 16 boar hairs were sampled, which turned out to be from 10 individual (7 females and 3 males) boars. We found that 9 pairs, out of 45 possible pairs, were most likely to be relatives. The result from kinship data and the location of the sampled hairs suggest that wild boars in this area live as family groups that consist of a mother and her offspring, which is consistent with known habits of wild boars. It is needed to include more samples and microsatellite markers for better precise estimation of kinship among the boar individuals.

• The Korean Journal of Zoology
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• v.33 no.4
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• pp.499-508
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• 1990

Surveys of elecirophoretic variations in isozymes and proteins encoded by 25 persumtive loci were conducted to estimate the degree of genic variation and to assess species recognition for three species of the Family Gasterosteidae (Pisces) in Korea. The average degree of genic variation of Gasterosteus aculeatus was HD = 0.086, HG = 0.084, and P =36% and it was similar to Pungitius sinensis (HD = 0.080, HG = 0.091, P = 29%). But P.kaibarae was less variable than these species (HD = 0.034; HG = 0.034, P = 13%). The average genetic similarities among the populations in each species of Gasterosteus aculeatus pungitius sinensis and P. kaibarae were S = 0.963, S = 0.953 and S = 0.800 respectively.Yongchon and Kyongiu populations of P. kaibarae were genetically remote from the rest of the conspecific populations (S = 0.690). Chae (1988) also observed morphological differences between them. The value of genetic similariiy coefficients of S = 0.606 between P. sinensis and P. kaibarae is low enough to consider as specific level of genetic difference, but moderate rate of hybridization (44%) between them at a synpatric area of Kangnung indicates that isolating mechanism is incomplete. Therefore it is concluded that P. sinensis and P. kaffarae are semispecitic status. Intergeneric genetic similarities between the genus Gasrerosteus and Pungitius were S = 0.052 and this value is the lowest estimate up to date in freshwater fishes.

• Animal Systematics, Evolution and Diversity
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• v.16 no.2
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• pp.203-211
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• 2000

Partial mitochondrial 16S rDNA and COI gene were amplified using PCR and sequenced for four species of oysters in Korea. Phylogenetic relationships among them were inferred from their aligned sequences by neighbor-joining method. The sequence comparison data of two mitochondrial genes showed that the genetic distinction between two oyster genera (Crassostreo and Ostrea) was obvious. Phylogenetic analysis based on the nucleotide sequences and A+T percentage of two genes indicates that C. gigas and C. nippona strongly formed a sister group and then C. ariakensis was clustered with the clade although that based on amino acid sequences of COI gene by neighbor-joining method represented different phylogenetic tree.

• Journal of Plant Biology
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• v.28 no.2
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• pp.95-104
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• 1985

Additional serological data from radial immunodiffusion and conventional immunoelectrophoresis with combination of presaturation of antibody were obtained for further interpretation of Cornus (and relatives) systematics. Pollen proteins were compared by qualitative and quantitative means. Cornus drummondi and C. stolonifera are very similar to C. florida, and C. amomum and C. recemosa are less similar. Nyssa constitutes the next distinct and most similar family, Nyssaceae to the Cornaceae. The serological affinities indicate that Corokia and Griselinia should not be included within the Cornaceae. Their taxonomic treatment to elevate to the family level is now awaiting until more data are accumulated.

• Journal of Applied Biological Chemistry
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• v.62 no.3
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• pp.287-292
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• 2019

Pseudomonas tolaasii causes brown blotch disease on the oyster mushroom (Pleurotus ostreatus). Various pathogenic strains of P. tolaasii were isolated and divided into three subtypes, $P1{\alpha}$, $P1{\beta}$, and $P1{\gamma}$. For phage therapy, bacteriophages against to these subtype strains were applied to mushroom cultivation and very successful to prevent from the disease. In this study, bacteriophages were isolated against the representative strains of subtype pathogens and their polyclonal antibodies were synthesized to investigate structural relationship among capsid proteins of phages. Phage preparations over $10^{10}pfu/mL$ were injected to rabbit thigh muscle and polyclonal antibodies were obtained after three times of boost injection. Titers of the antibodies obtained were over $2{\times}10^7Ab/mL$ for the phage ${\phi}6264$, $1{\times}10^6Ab/mL$ for the phage ${\phi}HK2$, and $1{\times}10^7Ab/mL$ for the phage ${\phi}HK19$ and phage ${\phi}HK23$. High specific activities were observed between antibodies and the corresponding bacteriophages. Some cross-reactivities between the antibodies and non-corresponding bacteriophages were also measured. Antibody $Ab{\phi}6264$ inactivated all phages of $P1{\alpha}$ subtype and only phage ${\phi}HK16$ among $P1{\beta}$ subtype phages. Antibody $Ab{\phi}HK23$ of $P1{\gamma}$ subtype neutralized all phages of $P1{\beta}$ subtype as well as the phage ${\phi}HK23$, showing the widest phage-inactivation range. When the structural-similarity studies of phages were investigated by using phage antibodies, closeness obtained by phylogenetic analysis of 16S rRNA genes of pathogenic strains were quite different from that of polyclonal antibody-specific structural similarity of phage capsid proteins. In conclusion, there is weak correlation between the host strain specificity of bacteriophage and its capsid structural similarity measured by phage antibodies.

• Korean Journal of Microbiology
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• v.50 no.2
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• pp.164-168
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• 2014

The ecosystem of the Arctic region has been increasingly affected by global warming. Archaeal ammonia monooxygenase alpha subunit coding gene (amoA) which is a key enzyme for nitrification was used to investigate the effect of runoff water of ice melt on microbial community of nitrogen cycle. The archaeal amoA genes at coastal area of Svalbard, Arctic region were PCR-amplified and sequenced after clone library construction. Analysis of archaeal amoA gene clone libraries suggested that the station 188 which is in the vicinity to the area of runoff water harbor lower ammonia-oxidizing archaeal diversity than the station 176 and 184. The average amino acid sequence identity within all archaeal amoA gene clones was 94% (with 91% nucleotide sequence identity). While all the clones of the station 188 were affiliated with Nitrosoarchaeaum clade containing strains isolated from low-salinity and terrestrial environments, about 45% of total clones of the station 176 and 184 were related to marine Nitosopumilus clade. Interestingly, other typical archaeal amoA gene clones of thaumarchaeal I.1b clade frequently retrieved from terrestrial environments was identified at station 188. Microbial community of nitrogen cycle in marine sediment might be affected by input of sediments caused by runoff glacier melt waters.

• Research in Plant Disease
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• v.26 no.3
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• pp.179-182
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• 2020

In July 2018, a serious rust symptom was found throughout the fringe trees planted in Gangjin-gun, Korea. Yellow and brown spots were observed on the adaxial (topside) surface of the collected fringe tree leaves, and yellow color aecia were observed on the abaxial (underside) surface leaves. The size of aeciospore and urediniospores of JCK-KCFR1 strain were measured to 41.2 ㎛ (Φ) and 28.84 ㎛ (Φ) with a light microscope. Phylogenetic analysis of the small subunit rRNA, internal transcribed spacer, and large subunit rRNA region indicated that JCK-KCFR1 strain is novel species of the genus Puccinia and closely related to Puccinia kusanoi, which has been reported a rust pathogen on bamboo. In May 2019, rust symptoms were also discovered on the bamboo leaves planted around the fringe tree on Muwisa-ro, and their telia and teliospores were observed on the abaxial leaf surfaces of the bamboo with 100% sequence homology with the rust of the fringe tree. This is the first report that Puccinia sp. JCK-KCFR1 is a new species that requires both primary (fringe tree) and alternative (bamboo) host plants to complete its life cycle in Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.790-799
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• 1997

Aegilops genus is known to include the donor species of the Band D genome of the bread wheat(ABD). An effort to establish a better strategy for phylogenetic relationships about Aegilops polyploids by AFLPs(Amplified Fragment Length Polymorphisms) was conducted using the 19 Aegilops sPP. and T. aestivum. The 207 polymorphic bands from the amplified products on the 6% acrylamide denaturing sequencing gels were obtained with the 7 AFLP primer combinations, and used to account for the genetic similarities and cluster analysis using NTSYS program. According to the genome analysis, the $M^h$-genome of Ae. heldreichii was estimated as an intermediate genome between the M-genome of Ae. comosa and N-genome of Ae. uniaristata and supposed to be incorporated in the establishing process of UM-genome as a possible diploid donor. And Ae. ventricosa(DN) was more close to Ae. umbellulata(U) than Ae. squarrosa(D). The close relationship between Ae. squarrosa and T. aestivum was perceived as a diploid donor of D-genome. As for the polyploid species, hexaploid Ae. triaristata was more closely related to Ae. columnaris rather than tetraploid Ae. triaristata. The clustered groups were, basically same to the previous Gihara's sections based on phenotypes and pairing analysis of interspecific hybrids. AFLP was evaluated as an efficient and powerful method in the genome evaluation of closely related species.