• Korean Journal of Animal Reproduction
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• v.22 no.4
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• pp.425-435
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• 1998

To improve the efficiency of nuclear transplantation in bovine, in this study the development in vitro of nuclear transferred (NT) embryos was compared by different activation regimens of the enucleated oocytes. The effect of developmental stage and culture system of donor nuclei on fusion and development in vitro of NT embryos were also evaluated. Oocytes were collected from Hanwoo ovaries obtained from slaughterhouse and matured in Ham's F-10 supplemented with hormones. After 20~22 h maturation, the oocytes were vortexed to be free from cumulus cells and subsequently their nucleus and the first polar body were removed. Enucleated oocytes were divided into 3 groups for activation; the oocytes of group I were activated with ionomycin for 5 min and subsequently incubated in 6-dimetylarninopurine (DMAP) for 4 h, Those of group II were treated with DMAP for 4 h at 39 h after onset of in vitro maturation (IVM) and those of group III were kept in room temperature ($25^{\circ}C$) for 3 h at 39 h after onset of IVM. After in vitro fertilization (IVF) the embryos for muclear donor were cultured either by group culture (20 embryos /50 ${mu}ell$ drop) or individually (1 embryo /50 ${mu}ell$ drop) for 4 day and 5 day. At day 4 and 5 after IVF, blastomeres were separated in calcium-magnesium free medium, and then classified into small (day 5: $\leq$ 38 ${\mu}{\textrm}{m}$, day 4: $\leq$ 46 ${\mu}{\textrm}{m}$) and large (day 5 : $\geq$ 38 ${\mu}{\textrm}{m}$, day 4 ; $\geq$ 46 ${\mu}{\textrm}{m}$). The separated blastomeres were replaced into enucleated and activated recipient cytoplasm. The blastomere-oocyte complexes were fused by electrically. The NT embryos were cultured in TCM-199 containing 10% FCS in 39$^{\circ}C$, 5% $CO_2$ incubator for 7 day. The results obtained were summarized as follows; There were no differences in fusion and development to blastocyst between groups as group I (68%, 10%), group II (75%, 14%) and group III (73%, 9%), respectively. However, the cell number in blastocyst of NT embryos in group III were significantly fewer than in the other groups (P＜0.05). No differences in fusion and development to blastocyst were found between individual or group cultured and between small or large blastomeres of day 4 and day 5 donor embryos. From these results, it was concluded that the combination of ionomycin and DMAP, or treatment of DMAP at 39 h after onset of IVM were useful for the efficient of production of NT bovine embryos, and the individual cultured embryos could be simply used as donor nuclei for NT bovine embryo.

• Research in Plant Disease
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• v.21 no.3
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• pp.235-242
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• 2015

A 2014 nationwide survey in radish fields investigated the distribution of common viruses and possible emerging viruses. Radish leaves with virus-like symptoms were collected and 108 samples assayed by RT-PCR using specific primers for Radish mosaic virus (RaMV), Cucumber mosaic virus (CMV), and Turnip mosaic virus (TuMV); 47 samples were TuMV positive, and RaMV and CMV were detected in 3 and 2 samples, respectively. No samples showed double infection of TuMV/RaMV, or RaMV/CMV, but two double infections of TuMV/CMV were detected. TuMV isolates were sorted by symptom severity, and three isolates (R007-mild; R041 and R065-severe) selected for BLAST and phylogenetic analysis, which indicated that the coat protein (CP) of these isolates (R007, R041, and R065) have approx. 98-99% homology to a previously reported TuMV isolate. RaMV CP showed approx. 99% homology to a previously reported isolate, and the CMV CP is identical to a previously reported Korean isolate (GenBank : GU327368). Three isolates of TuMV showing different pathogenicity (degree of symptom severity) will be valuable to study determinants of pathogenicity.

• Journal of the Korean Wood Science and Technology
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• v.36 no.6
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• pp.96-104
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• 2008

In this study, the observation of test pieces with an optical microscope was carried out after fabricating 4 groups of standard lacquer, in order to inquire into the ancient lacquering techniques. Group I had transparent reddish brown color, and the part of layer was indefinite, and the layer polished was easily distinguished. Group II had mostly transparent yellowish brown color, and it was possibility distinguished the layers when they were varnished with mixed lacquer after prime coating, and when they were varnished with lacquer as the prime coating. Group III set up membrane in the hardening process of the lacquer, and the lacquer and the unsaturated fatty acid of internal part was dried, so the top layer and the lacquer layer were separated and observed. Group IV: When the lacquer coat film of Group IV test pieces were observed in polarized light, the ferrous components were seen as the black and red color were mixed, and the silica crystals of silty soil were distinguished by reflection of lights. And the colors were distinguished as the lacquer layer of ruddle had red color, and the mixed lacquer of reddish lacquer and ruddle had the deep red color at the top and the bottom, but the middle part had the bright red color.

• Information Systems Review
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• v.10 no.1
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• pp.165-191
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• 2008

Traditionally, information systems audit has been carried out by the auditors. So the main focuses of audit are managerial point of view, such as finding errors or missing functions. The final purpose of audit, however, is to help the organization to make better decision through better information services. So we felt that managerial point of audit should be extended to include the factors that increase the competitiveness of organization. To accomplish this goal, new factors that reflect the ideas of three groups, users, developers in addition to auditors. First, by adopting the ideas from service quality evaluation area, we develop six items and 24 indexes for information systems audit. Second, we separate the audit related parties into three groups, users, developers and auditors. The main reason is that major concerns of three groups to the system will be different considerably. Third, we quantified the weights of each group to each 6 items and 24 indexes using Analytic Hierarchy Process. Fourth, the resulting weights of each item are found to be different by the group, and possible reasons are analyzed.

• Applied Biological Chemistry
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• v.50 no.2
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• pp.101-106
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• 2007

Twenty-two strains of nematophagous fungi were isolated from 100 soil samples. Nematophagous fungi were classified into three categories; 3-dimensional adhesive nets (A group), 2-dimensional adhesive nets (B group) and constricting ring (C group). Nine strains were selected and identified on the basis of morphological characteristics (hypha, conidiophore, form and size of conidia, number of conidia, node of conidophore, number and location of septa, size and color of chlamydospore) and ITS (internal transcribed spacer) region of rDNA sequences. As the results, the isolated were identified as belonging to the species of Monacrosporium thaumasium (Kan-2, Kan-4, Kan-11), Arthrobotrys oligospora (Kan-9, Kan-13, Kan-20, Kan-21), A. musiformis (Kan-12), and A. dactyloides (Kan-22).

• Journal of Convergence for Information Technology
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• v.10 no.3
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• pp.58-66
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• 2020

This paper aims to empirically analyze the factors of difference in trust level for Internet-only bank by age groups. The purpose of this study is to provide clues and a research data in developing differentiation strategies that can increase consumer's trust by age group. In this study, above all, the financial consumer group was divided into the 2030s and 4050s based on age. The study explored the trust determinants of the two groups and compared the differences between the two groups using a multi-group analysis method. As results, in all age groups, financial consumer's trust was seen as a determinant of convenience, security and benefit of financial transaction costs. The difference of the determinant of trust between 2030s and 4050s occurred in the factor of benefit. Moreover, it reconfirmed that consumer's trust is later strongly extended to relationship commitment. These findings suggest that trust is of paramount importance to the Internet-only bank, whose market competition will intensify in the future.

• Research in Plant Disease
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• v.24 no.2
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• pp.170-173
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• 2018

Three isolates of Cucumber mosaic virus isolated from Commelina communis plants showing chlorosis and mosaic were collected in Chungju and Chuncheon, Korea. To confirm genetic variation of these three isolates (CMV-Co, CMV-Co2, and CMV-Co3), we performed PCR-RFLP and sequence analysis. Sequences of coat protein genes of CMV-C0, -Co2 and -Co3 were compared with CMV-Fny and showed 96.3%, 96.3%, and 95.9% similarities, respectively. In host reactions, three CMV-Co isolates induced systemic necrosis in Cucurbita pepo unlike CMV-Fny and CMV-Co, CMV-Co2 and CMV-Co3 observed differential symptoms responses in Physalis angulata and Nicotiana rustica. These results indicated that three isolates of CMV isolated from C. communis have genetic and biological variation.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.48 no.6
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• pp.124-132
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• 2011

In a modern electronic warfare, radar signal environments become more denser and complex. Therefor the capability of reliable signal analysis techniques is required for ES(Electronic warfare Support) system to identify and analysis individual emitter signals from received signals. In this paper, we propose the new signal grouping algorithm to ensure the reliable signal analysis and to reduce the cost of the signal processing steps in the ES. The proposed grouping algorithm uses KDE(Kernel Density Estimator) and its CDF(Cumulative Distribution Function) to compose windows considering the statistical distribution characteristics based on the radar frequency modulation type. Simulation results show the good performance of the proposed technique in the signal grouping.

• Journal of KIISE:Information Networking
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• v.28 no.1
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• pp.143-153
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• 2001

본 논문에서는 에드-혹 망의 멀티캐스트 라우팅 프로토콜인 ODMRP(On-Demand Multicast Routing Protocol)를 확장한 PatchODMRP를 제안한다. ODMRP는 네트워크 상에서 멀티캐스트그룹의 송신원으로부터 수신원에 이르는 경로 상에 있는 노드들을 FG(Forwarding Group) 노드로 선출하여 이들이 해당 멀티캐스트그룹에 속하는 패킷을 모두 플러딩하도록 함으로써 멀티캐스트 그룹데이타 전송을 담당하는 메쉬를 구성하도록 하는 방안이다. 그런데 ODMRP는 주기적으로 이 메쉬를 구성하는 FG 노드들을 재 선정하기 때문에 이 주기가 길어지면 메쉬 구성이 네트워크 노드들의 이동성을 따라가지 못해 메쉬분리가 발생하고 데이터가 손실될수 있다. 반면에 이 주기를 짧게 하면 오버헤드가 지나치게 커질수 있다. 특히 송신원의 수가 적은 경우 ODMRP의 메쉬는 매우 성기게 형성되는데 이때 호스트들의 이동성이 크면 메쉬 연결을 유지하기 위하여 이 주기를 짧게 잡아주거나 높은 데이터 손실율을 감수해야한다. 본 논문에서는 이 문제점을 해결하고자 각 FG 노드들이 BEACON 신호를 이용해 자신에 인접한 메쉬에 손실이 발생한 것을 인지하고 이를 국부적인 플러딩을 통하여 빠르게 복구하는 메커니즘을 ODMRP에 추가한 PatchODMRP 방식을 제안한다. 시뮬레이션을 통하여 기존의 ODMRP와 제안하는 PatchODMRP의 성능을 비교한 결과 PatchODMRP가 호스트의 이동성에 훨씬 강하며 ODMRP에 비하여 낮은 오버헤드로 높은 데이터 전송률을 제공할 수 있음을 보여주었다.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.1
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• pp.21-26
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• 2013

In this paper we propose a system where we divide the group with 2 symbols. The two added symbols are separated by multiplexing and later added using the DE-multiplexing technique. In our proposed system a simple Partial Interference Cancelation (PIC) group decoding scheme is used for Double-ABBA(D-ABBA) Quasi-Orthogonal Space Time Code, which reduces the decoding complexity for the higher order Multiple Input Multiple Output (MIMO) space time block coding. Finally we compare the proposed scheme performance using the different modulation schemes.