• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.05a
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• pp.307-310
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• 2004

본 연구는 젖소 유방염의 환경성 원인체인 그람음성균의 분포와 E. coli의 항생제 내성 및 사람 식중독과의 연관성을 조사하기 위해 E. coli 혈청형에 대해 조사한 바, 2001년과 2003년 그람음성균의 주요 균 중 분포에서 많은 차이가 났으며, 2003년에는 E. coli가 31.8%로 가장 높은 것으로 나타났다. 체세포수에 따른 그람음성균의 분포율에서는 1백만 cells/ml 이하가 65.3%로 가장 높았으며, 체세포수 3백만 이상의 유즙에는 10.8%만이 분포하고 있는 것으로 나타났다. 또한, E. coli의 항생제 내성율 양상에서는 페니실린, 클록싸신, 세팔로틴등이 70%이상의 높은 내성율을 나타내었으며, E. coli 혈청형에서는 O23, O111, O157과 같은 주요 장관출혈성 E. coli 식중독균은 분리되지 않았다.

• Journal of Korean Ophthalmic Optics Society
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• v.13 no.1
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• pp.21-26
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• 2008

Purpose: To investigate the proper care system of sample-wearing cosmetic contact lens (SWCCL). Methods: Research on the actual condition about SWCCL was examined in fifty cosmetic contact lens wearer in their 10s and 20s by the questionnaire. Also, the extents of the contamination of gram-negative bacteria and fungi were measured in SWCCL after wearing for 2, 4 and 6 weeks, and it was investigated whether the proper care system could prevented the contamination. Results: As the result of the questionnaire, people purchased the cosmetic contact lens after trying SWCCL with a mean of 4.5. The contamination of gram-negative bacteria and fungi were significantly increased in SWCCL-wearing period-dependent manner. In both Group 1 (rubbing SWCCL and exchanging preserving solution every wearing of SWCCL) and Group 2 (only exchanging preserving solution every wearing of SWCCL without rubbing SWCCL), the contamination of gram-negative bacteria and fungi were prevented perfectly. In the case of Groups having every-week care, the proliferation of gram-negative bacteria and fungi were somewhat suppressed, and rubbing was helpful of decreasing the contamination. The biweekly care had scarcely any effect for preventing the contamination. Conclusions: By exchanging the preserving solution every wearing of SWCCL, the contamination of gram-negative bacteria and fungi could be suppressed perfectly.

• The Korean Journal of Food And Nutrition
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• v.19 no.4
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• pp.526-531
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• 2006

Minimum inhibition concentration(MIC), growth inhibition activity, and colony forming inhibitory activity of grapefruit seed extract against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Escherichia coli, Salmonella enterifidis and Serratia marcescens were tested. MIC of grapefruit seed extract against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Escherichia coli, Salmonella enteritidis and Serratia marcescens was 12.5, 12.5, 12.5, 50, 50, 100ppm, respectively. Growth inhibition concentration of grapefruit seed extract against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Escherichia coli, Salmonella enteritidis and Serratia marcescens was below 1.0, 6.25, below 1.0, 6.25, 25, 25ppm, respectively. Colony forming inhibitory activity of grapefruit seed extract against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Escherichia coli, Salmonella enteritidis and Serratia marcescens was 93.9, 94.0, 99.9, 4.4, 82.7, 86.4%, respectively. Colony forming inhibitory activities of grapefruit seed extract against Gram positive bacteria were higher than that against Gram negative bacteria.

• 건강소식
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• v.14 no.7 s.140
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• pp.31-31
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• 1990

• Korean Journal of Food Science and Technology
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• v.22 no.6
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• pp.716-719
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• 1990

The essential oils were isolated by steam distillation from 13 spices used for curry. Antimicrobial activity of essential oils for two strains of Gram(+) bacteria, Gram(-) bacteria, lactic acid bacteria, yeast and mold were investigated by agar diffusion method. 5 spice essential oils(clove, cumin, nutmeg, oregano, rosemary) having high antimicrobial activity were selected and their minimal inhibitory concentration(MIC) were measured. Very low concentration ($0.2{\sim}9\;mg/ml$) of 5 spice essential oils were sufficient to prevent microbial growth. The data show that Gram(+) bacteria were more sensitive to the antimicrobial compounds in spices than Gram(-). But though Gram(+) bacteria, lactic acid bacteria were less sensitive to the compounds than Gram(-).

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.10a
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• pp.388-390
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• 2004

본 연구는 자돈 설사의 원인이 되는 대장균, 살모넬라 등 그람음성균을 억제할 수 있는 유산균의 이용을 위해 선발된 균주의 과산화수소 대사를 구명하기 위해 수행하였다. 대장균 억제유산균중 superoxide dismutase 활성이 우수한 균주가 관찰되었으며, 과산화수소 이외에 그람음성균 억제에 유효한 bacteriocin-like compounds의 존재 유무, 대사과정에서 과산화수소 생성과 관련된 다른 효소 활성, superoxide dismutase 유전자 클로닝에 관한 연구가 진행중이다.

• Proceedings of the Korean Society of Veterinary Service Conference
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• 1997.05a
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• pp.242.1-242
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• 1997

• Pediatric Infection and Vaccine
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• v.4 no.1
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• pp.79-89
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• 1997

Purpose : Neonatal bacterial meningitis is the disease which clinical manifestations are nonspecific and several neurologic complications may occur. We studied neonatal bacterial meningitis, particularly in treatment and prognosis according to causative organisms -gram positive and gram negative bacteria- to assist in treatment of neonatal bacterial meningitis. Methods : We analysed twenty-four cases retrospectively who had been admitted in NICU or pediatric ward in Chung-ang Gil hospital from Jan. 1991 to Jun. 1996, and who had proven causative organisms in culture or latex agglutination[n test in CSF. Results : 1) The ratio of male to female was 2.4: 1. The mean birth weight and gestational age in cases with gram positive bacterial meningitis were $2.91{\pm}0.79kg$ and $38.4{\pm}2.74$ weeks and those in cases with yam negative bacterial meningitis were $3.30{\pm}0.90kg$ and $37.7{\pm}3.33$weeks respectively. There was no significant difference between the two groups. 2) The perinatal predisposing factors were pematurity, mecoinium staining amnionic fluid, matemal diabetes and pregnancy-induced hypertension, etc. The clinical manifestations Were fever, seizure, poor oral intake and fontanel bulging, etc. There were eleven cases with early onset bacterial meningitis(four cases by gram positive bacteria, seven cases by gram negative bacteria), and thirteen cases with late onset bacterial meningitis(seven cases by gram positive bacteria, six cases by gram negative bacteria). There was no significant difference between the two groups in terms of onset. 3) There were eleven cases with yam positive bacterial meningitis and they were coagulase-negative staphylococci(three cases), group B streptococci(three cases), Staphylococcus aureus(two cases), Streptococcus viridans(two cases), and enterococci(one case). And there were thirteen cases with gram negative bacterial menir gitis and they were Escherichia coli(seven cases), Klevsiella pneumoniae(three cases), Pseudomonas aeruginosa(one case), Acinetobactor(one case) and Enterobacter(one case). 4) The initial CSF WBC counts in cases with yam negative bacterial meningitis were significantly higher than those in cases with gram positive bacterial meningitis but the CSF protein and glucose levels were no significant difference in the two groups statistically. 5) The number of cases with abnormal findings in brain ultrasonography was seven in gram positive bacterial meningitis and ten in gram negative bacterial meningitis. 6) There were relatively high sensitivity to penicillin derivatives, the first generation cephalosporin and vancomycin in gram positive bacteria and to the third generation cephalosporin and amikacin in gram negative bacteria. 7) The mortality rate was 20.8%(5 cases were expired or discharged hopelessly). There was no significant difference between the two groups in prognosis. Conclusions : We recommend active treatment in noenatal bacterial meningitis to improve prognosis because the prognosis is poor.

• The Korean Journal of Mycology
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• v.23 no.2 s.73
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• pp.176-189
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• 1995

For the development of antibiotics from Korean mushrooms, the biological activities of extracts from 98 species of mushrooms and from 25 mushrooms were tested against 9 different Gram-negative bacteria and 8 fungi, respectively. Fruiting bodies of each mushrooms were extracted with petroleum ether (P), 80% ethanol (E), and distilled water (H) in that order. P, E, or H extracts from 20 mushroom samples exhibited the antibacterial activity against 8 different Gram-negative bacteria containing Klebsiella pneumoniae, selectively. Among the mushroom extracts with antibiotic activity, E extracts of Boletus umbriniporus, Armillariella tabescens, Rhodophyllus sinuatus, and Suillus luteus showed various antibiotic activities against several bacteria. E extracts of Abortiporus biennis, Phellinus gilvus, and Polyporus dispansus are highly active against Salmonella typhi and their minimum inhibitory concentration (MIC) was all $10\;{\mu}g/ml$. E extract of Armillariella tabescens showed the antifungal activity against Trichopyton mentagrophytes, and its MIC was $300\;{\mu}g/ml$.

• Polymer(Korea)
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• v.30 no.5
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• pp.397-401
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• 2006

In this study, we coated the polypropylene filter with titania sol and investigated various characteristic such as its deodorization efficiency and sterilization effect of gram negative bacillus Escherichia coli(ATCC 25922) and gram positive bacillus Staphylococcus aureus(ATCC 6538). As the results, in case of titania sol coated polypropylene filter, the deodorization rate of ammonia, trimethylamin and acetic acid which cause indoor air pollution was 66%, 90% and 16% respectively. Also. the sterilization rate of gram negative bacillus Escherichia coli(ATCC 25922) and gram positive bacillus Staphylococcus aureus(ATCC 6538) was 99.9% after 24 hours.