• Title/Summary/Keyword: 균학적 특성 비교

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젖산생성균의 .betha.-galactosidase의 생화학 및 분자생물학적 특성

  • 민해기
    • The Microorganisms and Industry
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    • v.19 no.1
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    • pp.8-17
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    • 1993
  • 젖산생성균의 .betha.-gal의 생성과 Bif. longum KCTC 3215에 의한 .betha.-gal 생산, 정제 및 특성에 관한 연구와 젖산생성균의 .betha.-gal 유전자의 클로닝 및 대장균에의 발현과 Str.thermophilus SKD 1006의 Lac Z 유전자를 비교 분석하였다.

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Induction of Prochloraz-Resistant Isolates of Botrytis cinerea in Vitro and Their Biological Properties (Prochloraz에 저항성인 잿빛곰팡이균(Botrytis cinerea)의 유기 및 이들 균의 생물학적 특성)

  • 김병섭;박은우;박정헌;노성환;조광연
    • Korean Journal Plant Pathology
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    • v.12 no.2
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    • pp.226-230
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    • 1996
  • 1994~1995년 사이에 대전, 공주, 논산, 부여와 김해 등지에서 채집한 병든 딸기, 토마토, 오이에서 총 1,504 균주의 잿빛곰팡이병균을 분리하여 10 $\mu\textrm{g}$/ml의 약제를 넣은 PDA 배지에서 배양하면서 약제반응을 조사한 결과, benomyl에 대하여는 1,147(76.3%)균주, procymidone에 대하여는 614(40.8%)균주, prochloraz에 대하여는 189(12.6%)균주가 저항성으로 나타났다. UV 처리 및 약제 배지에서의 누대배양을 통하여 prochloraz저항성 균주를 선발하였는데, 이들의 생육 억제 최소 농도(MIC)는 8 $\mu\textrm{g}$/ml 이상이었고, 감수성균의 MIC는 2 $\mu\textrm{g}$/ml 이하였다. 병원력, 포자 발아율, 균핵형성과 같은 적응력과 관련된 생물학적 특성 비교에서 저항성 균주들은 감수성 균주보다 열등하거나 비슷한 정도로 나타났다.

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Characteristics Comparisons of Edwardsiella tarda Isolated from Cultured Olive Flounder and Eel (양식넙치와 뱀장어에서 분리된 Edwardsiella tarda의 특성 비교)

  • Kim, Eunheui
    • Journal of fish pathology
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    • v.34 no.1
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    • pp.31-38
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    • 2021
  • The objective of this study was to determine comparative biochemical characteristics and RAPD (random amplified polymorphic DNA) profiles of 18 strains of Edwardsiella tarda isolated from cultured olive flounder (Paralichthys olivaceus) and eel (Anguilla spp) that showed diseases between 1996 and 2010 in Korea. In terms of biochemical properties, they showed four patterns with differences in citrate degradation and production of H2S and indole. All strains were identified as E. tarda. Characteristics of isolates were not classified according to their host. As a result of PCR with E. tarda-specific primer, EDtT, the same band of about 270 bp was detected in all 18 isolates. However, no specific band was detected in type strains of E. tarda or Edwardsiella ictaluri. As a result of RAPD PCR performed with primer No. 5 and No. 6 of a Ready-To-Go-RAPD kit, the band profile showed clear differences among isolates of olive flounder, isolates of eel, and E. tarda and E. ictaluri type strains. It was possible to organize their characteristics according to the origin of host with possibility to develop tools for pathogen monitoring.

사상균이 생산하는 Xylanase에 관한 연구 제1보 Xylanase 생성과 그 성질

  • Bae, Moo;Kim, Byung-Hong;Lee, Gye-Jun;Kang, Kyung-Hee
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1978.04a
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    • pp.97.2-97
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    • 1978
  • 숙성한 퇴비에서 16종의 사상균을 분리하고 이들의 xylanase 및 cellulase의 활성을 측정하였다. 이 결과 효소활성이 강한 6 균주를 선별하고 이들의 형태학적 특성을 속까지 동정하고 선별된 균주가 생산하는 xylanase와 cellulase의 성질을 비교 검토하였다. 선별된 균주의 배양기질에 따른 효소생산량 산량을 비교하기 위해 cellulose와 xylan으로 배양한 후 이들을 분해하는 효소활성의 비 즉 xylanase/cellulase 비를 계산하고 이들 효소의 일반 성질을 검토하였다. 차후 연구에서 이들 효소를 분리, 정제하기 위해 acetone에 의한 침전성을 아울러 실험하였다.

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The Analysis of Scattering Characteristics of a Prime-focus Offset Parabolic Antenna with a Shaped Edge Structure (정형 모서리 구조를 갖는 옵\ulcornerV 파라볼릭 안테나의 산란 특성 해석)

  • 박대성;김형규;최재훈
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.10 no.3
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    • pp.412-418
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    • 1999
  • The scattering characteristics of a prime-focus offset-parabolic antenna are analyzed using UTD. First, ray tracing method is used to locate the shadow boundaries, and then UTD is utilized to evalute the far-zone scattered magnetic field pattern. The field components included in the UTD analysis are the reflected, edge diffracted and creeping waves. The effects of circular caps attached to both edges of a prime-focus offset-parabolic antenna are investigated by comparing the scattered magnetic field patterns with those of a knife edge parabolic reflector.

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The study on the causal agent of Streptococcicosis (Lactococcus garvieae), isolated from cultured marine fishes (해산 양식어류로부터 분리된 연쇄구균증의 원인균, Lactococcus garvieae에 대한 연구)

  • Lee, Deok-Chan;Lee, Jae-Il;Park, Chan-Il;Park, Soo-Il
    • Journal of fish pathology
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    • v.14 no.2
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    • pp.71-80
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    • 2001
  • The purpose of this study was to investigate the microbiological characteristics and the distributions of the bacteria causing streptococcicosis occurred in marine fish farm, Korea. Many kinds of cultures fishes suffered from the disease accompanied with typical symptoms, including darkening of the skin, exophthalmia, petechiae inside of the opercula and distended abdomen. The isolates from the diseased fishes were compared with Lactococcus garvieae by biochmical, biophysical and serological methods and polymerase chain reaction(PCR) assay. We isolated 35 strains of the geuns Streptococcus from the diseased olive flounder, Paralichthys olivaceus, yellow tail, Seriola quinqueradiata and Korean rockfish, Sebastes schlegeli. 15 strains out of the isolates were identified to L. garvieae and the others were not because of their different biochemical and biophysical charateristics. Seven strains of the isolates were agglutinated by rabbit serum raised against L. garvieae $KG^+$ phenotypic cells(ATCC49156)as a reference strain. Twenty-one strains of the isolates identified to L. garvieae since they were formed the expected band through performing PCR assay using specific primers, pLG-1(5'-CATAACAATGAGATCGC-3') and pLG-2(5'-GCACCCCGCGGTTG-3'). In the present study, it showed that L. garvieae was a dominant strain causing streptococcicosis in the tested area due to occurrence of 21 strains as L. garvieae out of all the isolates, 9 atrains as Streptococcus sp. and 5 strains as Enterococcus sp.

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Physiological and Biochemical Properties of Isolates of Rhizobia from Soybean (콩에서 분리한 근류균의 생리, 생화학적 특성)

  • 박기선;최재을
    • Korean Journal Plant Pathology
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    • v.12 no.3
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    • pp.351-357
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    • 1996
  • 콩으로부터 분리한 140균주의 근류균은 25균주(17.9%)가 Rhizobium fredii로 115 균주(82.1%)가 Brady-rhizobium japonicum으로 동정되었다. R. fredii에 속하는 분리 균주의 생존 pH 범위는 4..5∼9.0이었고 B. japonicum의 생육 pH 범위는 5.5∼8.0로 비교적 좁게 나타났다. B. japonicum에 속하는 98균주 중에서는 53균주(54%)가 IAA를 생산하지 않는 GT I group으로, 45균주(46%)는 IAA를 생산하는 GT II group으로 명확하게 구분되었으며, 항생물질에 대한 내성 유무에 의해 10개의 group으로 구분되었다.

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Isolation of Bacillus sp. SW29-2 and Its Antifungal Activity against Colletotrichum coccodes (Bacillus sp. SW29-2의 분리 및 Colletotrichum coccodes에 대한 항진균 활성)

  • Han, Yeong-Hwan
    • Journal of Life Science
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    • v.27 no.6
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    • pp.688-693
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    • 2017
  • Antifungal bacterium against Colletotrichum coccodes causing black dot disease of potatoes and anthracnose of tomatoes was isolated from sewage sludge. The isolate showed a 99% sequence homology of partial 16S rRNA of Bacillus methylotrophicus CBMB205 and Bacillus amyloliquefaciens subsp. plantarum FZB42. The isolate was identified as Bacillus sp. SW29-2, using the neighbor-joining phylogenetic tree, BlastN sequence analysis, and morphological and cultural characteristics. Bacillus sp. SW29-2 is an aerobic, Gram-positive, endospore-forming bacterium, of which the morphological and physiological characteristics were the same as those of type strain B. lichniformis CBMB205, except for the cell growth of over 4% NaCl. The cell growth of the temperature and the initial pH of the medium was shown at $18-47^{\circ}C$ (opt. ca. $38^{\circ}C$) and 3-9 (opt. ca. 6.0), respectively. The inhibition size (diameter) of Bacillus sp. SW29-2 against four strains of C. coccodes ranged from 23 to 29 mm. Also, the isolate showed antifungal activity against penicillium rot-causing Penicillium expansum in apples. Thus far, any report on the antifungal activity of Baciilus spp. against C. coccodes has not been found. These results suggest that the Bacillus sp. SW29-2 isolate could be used as a possible biocontrol agent against C. coccodes, and further applied to other plant pathogenic fungi.

Studies on the biochemical and serological characteristics of Edwardsiella tarda isolated from cultured flounder, Paralichthys olivaceus (양식넙치에서 분리된 Edwardsiella tarda의 생화학적 및 혈청학적 특성에 관한 연구)

  • Bang, Jong-Deuk;Chun, Seh-Kyu;Park, Soo-Il;Choi, Yun-Jung
    • Journal of fish pathology
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    • v.5 no.1
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    • pp.29-35
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    • 1992
  • This study was carried out in order to identify the biochemical and serological characteristics of Edwardsiella tarda isolated from cultured flounder, Paralichthys olivaceus in the east and south coast of Korea. During the year of 1990 and 1991, the number of isolated E. tarda were 131 strains. To identify the biochemical characteristics of them kinds of tests were conducted. The results represented that all the strains had the same biochemical characteristics, and their biochemical characteristics were no differences among strains. A serological analysis was carried out based on agglutination test with antiserum belonging to E. tarda serotype a (E-22), b (SU-138), c (SU-100) classfied in japan. The selected 10 isolates showed agglutinin titer of 5120-2560, 40-80 and below 40 against E. tarda serotype a, b and c, respectively. Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) profiles of cell proteins of selected 10 isolates were showed no differences in kinds and volumes of proteins among strains.

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Biological Control of Blue Mold by Microorganism (잿빛 곰팡이병의 미생물학적 제어)

  • 조정일;조자용;안병렬
    • Korean Journal of Organic Agriculture
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    • v.8 no.3
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    • pp.121-130
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    • 2000
  • In order to screen the antagonistic bacteria which inhibit the growth of the plant pathogen, Penicillum expansum, we isolated an effective bacterial strain and investigated into the antifungal activity of the antagonist and it's identification. The eleven strains of bacteria which strongly inhibited P. expansum were isolated from the nature, and the best antagonistic bacterial strain designated as KB22, was selected. The antagonistic strain KB22 was identified to be the genus Bacillus subtilis based on morphological and biochemical characterization, The KB22 showed 55.9% of antifungal activity against the growth of P. erpansum. By the treatment of the culture broth and the heat treated culture filtrate of it, the B. subtilis KB22 showed 90% and 15% of antifungal activity, respectively.

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