• Journal of Forest and Environmental Science
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• v.15 no.1
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• pp.77-88
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• 1999

This experiment was carried out to compare the cultural characteristics of mycorrizal fungi associated with Pinus species, and to form mycorrhizal association with Pinus thunbergii by artificial inoculation of these fungi. Mycorrhizal fungi tested showed great variations in cultural characteristics. Most fungal isolates was best grown on MP medium, except PDA for Lepista sp.(Ln73/92). Hagem for Rhizopogon rubescens(FRI91017), and FDA for Paxillus sp.(Pa60/92). Optimum temperature for these fungi was $25^{\circ}C$, except $30^{\circ}C$ for Pisolithus tinctorius(FRI91004 and Pt1). The range of pH conditions favorable for these fungal isolates were also variable from weak acidic(pH5) to weak alkalic(pH8). Utilization of the carbon sources for these mycorrhizal fungi was different. Fructose, glucose, and maltose were all utilized well, while xylose was not utilized generally. Mycelial growth on the media supplemented with potassium nitrate was better than those on other media with urea, asparagine, or peptone as a nitrogen source, and the poor growth was observed on the media with urea. Pisolithus tinctorius(Pt1) among 7 mycorrhizal fungi artificially inoculated for the mycorrhizal synthesis on pinus thunbergii seedlings in the test tube containing a mixture of peat moss-vermiculite(2:1, v/v) formed mycorrhizae successfully after 3 months. P. tinctorius formed branched and unbranched roots covered with thick fungal mantle and radiating extemal hyphae. Mycorrhizal root cross-sectioned by hand, stained, and observed by Nomarski interference microscope showed typical characteristics of ectomycorrhizae: fungal mantle on epidermal cells and thick Hartig net hyphae around cortex cells.

• The Korean Journal of Mycology
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• v.20 no.2
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• pp.126-133
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• 1992

Using the soils containing several arbuscular mycorrhizae, the degrees of infection on the plant roots were measured with the different level of phosphate added on pot cultures. Infection on the plant roots was independent of the phosphate level for the roots of sorghum, but formation of arbuscular mycorrhizae in the roots was inversely related to the growth of soybean roots. It was concluded that infection of arbuscular mycorrhizae would be related to the phosphate level in the soils. Plants, themselves, were considered to control the infection of arbuscular mycorrhizae under the environments of soils, especially amount of phosphate.

• Journal of Mushroom
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• v.18 no.1
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• pp.1-9
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• 2020

Mycorrhiza refers to the association between a plant and a fungus colonizing the cortical tissue of the plant's roots during periods of active plant growth. The benefits afforded by plants from mycorrhizal symbioses can be characterized either agronomically, based on increased growth and yield, or ecologically, based on improved fitness (i.e., reproductive ability). In either case, the benefit accrues primarily because mycorrhizal fungi form a critical linkage between plant roots and the soil. The soilborne or extramatrical hyphae take up nutrients from the soil solution and transport them to the root. This mycorrhizae-mediated mechanism increases the effective absorptive surface area of the plant. There are seven major types of mycorrhizae along with mycoheterotrophy: endomycorrhizae (arbuscular mycorrhizae, AM), ectomycorrhizae (EM), ectendomycorrhizae, monotropoid, arbutoid, orchid, and ericoid. Endomycorrhizal fungi form arbuscules or highly branched structures within root cortical cells, giving rise to arbuscular mycorrhiza, which may produce extensive extramatrical hyphae and significantly increase phosphorus inflow rates in the plants they colonize. Ectomycorrhizal fungi may produce large quantities of hyphae on the root and in the soil; these hyphae play a role in absorption and translocation of inorganic nutrients and water, and also release nutrients from litter layers by producing enzymes involved in mineralization of organic matters. Over 4,000 fungal species, primarily belonging to Basidiomycotina and to a lesser extent Ascomycotina, are able to form ectomycorrhizae. Many of these fungi produce various mushrooms on the forest floor that are traded at a high price. In this paper, we discuss the benefits, nutrient cycles, and artificial cultivation of mycorrhizae in Korea.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.5
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• pp.341-349
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• 2004

This study was conducted to compare the effects of arbuscular mycorrhizal fungi (AMF) on the growth and fruit yield of hydroponically grown cucumber and tomato plants in solid medium culture. Mycorrhizal fungus Glomus sp. was collected from plastic film house soils of cucumber and tomato and inoculated to the experimental crops at the time of seeding and transplanting. Root infection of cucumber and tomato plants by AMF was more significantly increased when the AMF was inoculated at seeding stage than at transplanting stage. In the infected roots of cucumber and tomato, mycorrhizal hyphae was easily observed but vesicle and arbuscule were rare. Overall plant growth was increased with AMF inoculation and the growth was higher when AMF was inoculated at seeding stage. Fresh weight of each fruit of cucumber and tomato and sugar content in tomato fruits were significantly increased with AMF inoculation at seeding stage. The AMF inoculation also increased fruit yields of cucumber and tomato.

• Journal of Forest and Environmental Science
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• v.19 no.1
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• pp.99-107
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• 2003

For the investigation of the potentials of ectomycorrhizal (ECM) fungi on pine seedlings for reforestation on fire-damaged forest lands, six months old seedlings of Pinus densiflora, which were artificially inoculated or non-inoculated with Pisolithus tinctorius, were transplanted into the fire-damaged forest land. Seedling mortality was recorded as 3.5% for the seedlings inoculated with P. tinctorius at three months after transplanting, while it was 70.5% for the non-inoculated seedlings. Mycorrhizal root formation was shown as typically Y-branched type, and thier rate was 82% for inoculated seedlings, but it was 52% for non-inoculated. Comaprisons in seedling height, fresh weight, and dry weight between pine seedlings inoculated or non-inoculated with P. tinctorius at every 3 months till 9 months after transplanting showed that the inoculated seedlings were much better in all criteria as compared to the non-inoculated at the level of 63%, 35%, 18% in seedling height, 206%, 114%, 70% in fresh weight, and 187%, 109%, 63% in dry weight, respectively. The percentages were decreased by the elapse of time, which indicate that P. tinctorius give a growth-stimulating effects on seedlings at the early stage. T/R ratio for inoculated and non-inoculated seedlings after 9 months was 1.75 and 1.90, respectively.

• Journal of Korean Society of Forest Science
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• v.94 no.1 s.158
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• pp.16-20
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• 2005

Tricholoma matsutake ectomycorrhizas are unique in their morphology: not bifurcated broom shaped roots with not easily wettable brilliant and profuse white hyphae. To understand these characteristics the ectomycorrhizas were investigated with electron microscopy. T. matsutake ectomycorrhiza have thin mantle and typical Hartig net development in the epidermis and cortex, but no fungal mantle on the root apex. There were no penetrating hyphae inside of the cells of either epidermis, cortex or stele. Inside of the walls of epidermis and cortex cells are lined with ca. $2{\mu}m$ hemispherical amyloplasts. The brilliant hyphal surface was covered with various fine amorphous granules. The hyphal cell wall was thin membrane less than $0.3{\mu}m$ thick. There is no clamp connection on the hyphae. This thin membraneous cell wall with high elasticity can be related to survival strategy of the species without plasmolysis under frequent soil water stress environment. And the coarse hyphal surface with some water repellency can control sudden inrush of water of the hyphae with an extremely low osmotic potential. It is concluded that no mantle on the tip can induce mycorrhizas not bifurcated and that finely coarse surface of T. matsutake hyphae can make the hyphae brilliantly white but less wetted.

• Journal of Korean Society of Forest Science
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• v.94 no.3 s.160
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• pp.146-152
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• 2005

For the application of ectomycorrhizal seedlings on damaged slope lands, studies on cultural characteristics of Lyophyllum shimeji and ectomycorrhizal associations of Lespedeza cyrtobotrya seedlings were carried out by artificial inoculation of L. shimeji. Mycelial growth of L. shimeji was best on MP (1% malt extract, 0.1% peptone, 1% glucose and 1.5% agar) medium. An optimum temperature and pH for the mycelial growth were $25^{\circ}C$ and pH6, respectively. Mycorrhizal root of L. cyrtobotrya seedlings inoculated with L. shimeji showed characteristics of ectomycorrhizas with Hartig net. Growth rate of the mycorrhizal seedlings's roots was higher than that of non-mycorrhizal seedlings. When the mycorrhizal seedlings were transplanted in slope land, survival rate and dry weight were 62% and 850 mg/seedling, respectively. On the other hand, survival rate and dry weight of non-mycorrhizal seedlings were 11% and 430 mg/seedling, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.6
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• pp.1002-1007
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• 2010

The study was performed to investigate the influence on growth and development of grape-cuttings by arbuscular mycorrhizal (AM) fungi inoculation, AM colonization rate, and the phenomena of mycorrhizal association. Among the grape-cuttings, 'Kyoho' and 'Tamnara' cultivars inoculated with AM fungi showed significantly increase of leaf area, leaf number, total root length and root surface area than non-infected ones. But 'Cambell Early' did not showed any significant difference in total root length and root surface area even after the inoculation. The AM colonization rates in mycorrhizal inoculation treatment were 22.5-32.5% in total average after 8weeks, and were 29.6%, 28.8%, and 48.8% for 'Cambell Early', 'Tamnara', and 'Kyoho' respectively after 12weeks. The AM colonization rate marked very low level in non-colonization control plot.

• Journal of Korean Society of Forest Science
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• v.86 no.4
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• pp.466-475
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• 1997

I examined arbuscular mycorrhizal(AM) fungus inoculation effects on the seedling growth of Korean ash tree(Fraxinus rhynchophylla Hance), which distributes in fertile mesic soils, under a seven-day watering cycle of water stress and compost-added fertile conditions. Three Korea-native AM fungi were inoculated : an unidentified Glomus species, Gigaspora margarita Becker & Hall and Scutellospora heterogama(Nicol. & Gerd) Walker & Sanders from disturbed forest soils. The effect of AM fungus inoculation on the seedling varied depending upon fungal species and soil conditions. AM formation was 27 to 65% by the Glomus without forming spores, 47 to 74% with about 10 spores per 20g soil by G. margarita and about 65% with 35 spores by S. heterogama. The soil conditions did not affect either AM or spore formation. The Glomus inoculation increased shoot N and P concentrations, but did not affect seedling growth. G. margarita increased shoot N and P, irrespective of soil conditions, in general, but S. heterogama increased N under water stress and Pin the control soil only. These two fungi significantly increased seedling growth in both control and water stress soils. Compost addition increased the growth of non-mycorrhizal seedlings and offset AM fungus inoculation effects. The relative field mycorrhizal dependency(RFMD) of the seedlings was significant only in control and water stress soils by over 40% in G. margarita or S. heterogama AM plants. Under water stress RFMD was the most evident in S. heterogama AM plants. I conclude that some AM fungi such as G, margarita and S. heterogama can broaden the niche of Korean ash seedlings to a water stress or nutrient poor site but less likely to more fertile sites.

• The Korean Journal of Mycology
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• v.39 no.1
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• pp.7-10
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• 2011

Orchid mycorrhizal fungi (OMF) were examined in roots of the six terrestrial species of orchids collected in Ulleung Islands. Seven OMF isolates from the roots of orchids were identified based on morphological and molecular characters. Internal transcribed spacer region of OMF DNA was amplified using basidiomycete-specific ITS primers, ITS1-OF and ITS4-OF. OMF beloning to Tulasnellaceae and Ceratobasidaceae was identified through molecular analysis.