• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.4
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• pp.544-548
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• 2005

The objective of this study was to determine the effect of die temperature and dimension on extraction pattern, extract yield, and crude saponin content of extruded white ginseng. The extrusion variables were die temperature $(110\;and\;120^{\circ}C)$ and die dimension (3 holes with 1.0 mm, 2 holes with 2.0 mm, and 1 hole with 3.0 mm diameter). The browness and redness were indicator of active components in ginseng extract. Both were used to evaluate the effect of die temperature and die dimension on release pattern and release rate constant. Browness and redness of extract achieved its lowest value at die temperature $110^{\circ}C$ and 2 holes with 2.0 mm diameter, indicating the lowest extraction rate constant. Extract yield highly increased by extrusion treatment. Extract yield and crude saponin content were the highest at die temperature $120^{\circ}C$ and 1 hole with 3.0 mm diameter. In conclusion, extrusion process has contributed significantly in improvement of release rate of its active components.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.10 no.3
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• pp.151-162
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• 1977

Distribution of marine algae is diverse in Korea and the resource of edible algae is abundant marking 239,037 tons of yearly production in 1976. They have been known as a protein source and used as a supplement in Korean diet. It is necessary to estimate the potentiality and properties of usable algal proteins especially as food resources and studies of extraction and separation of the proteins, therefore, are basically required for this purpose. In this study, the influence of various factors including the sample treatment, extraction time and temperature, sample us extraction solvent ratio and pH upon the extractability of the water soluble protein was determined. And the effect of precipitation treatment for isolation of the algal protein from the extracts was also tested. Nine species of algae, the major ones in consumption as food namely Porphyra suborbiculata, Undaria pinnatifida, Hizikia fusiforme, Sargassum fulvellu, Enteromorpha linza, Codium fragile, Sargassum kjellmanianum and Ulva pertusa were collected as fresh from Kijang, Yangsan Gun, in the vicinity of Busan city. The content of crude protein $(N\times6.25)$ of the algae ranged from $9.46\%\;to\;24.14\% showing the highest value in Porphyra suborbiculata and the minimum in Hizikia fusiforme. In the effort of maceration of blending methods on the extractability, immersion freezing in dry ice-methanol solution appeared most effective yielding 1.5 to 2.5 times extractability than that of the mortar grinding method. The effect of the ratio of sample vs solvent on extractability differed from species. It was enhanced at the ratio of 1:20 (w/v) in Ulva pertusa and Enteromorpha linza while the ratio was 1:30 (w/v) for Cedium fragile, Undaria pinnatifida, Hizikia fusiferme, Sargassum fulvellum and Porphyra suborbiculata and 1:40 for Sargassum kjellmanianum respectively. The effect of extraction time and temperature was revealed differently from species which might be caused by differences in the constitution of algal tissues resulting in that the extraction for 1 hour at $50^{\circ}C$ gave the maximum extractabilily in Ulva pertusa and Enteromorpha linza, 2 hours in Porphyra suborbiculata, Hikikia fusiforme, Undaria pinnatifida, Sargassum kjellmanianum and 3 hours in Codium fragile. And the extractability was higher at $50^{\circ}C$ to $60^{\circ}C$ for the most of the tested samples except Hizikia fusiforme. The optimum pH for the extraction was 9 to 12. The recovery of extractable nitrogen to the total nitrogen was $63\%$ in average with the first extracts and $8.6\%$ with the second extracts respectively. Both extracts were prepared by 2 hour extraction at $50{\pm}1^{\circ}C$ with dry ice-methanol frozen and seasand macerated materials. And these conditions assumed to be an optimum for the extraction of water soluble algal proteins since the nitrogen content after the first extraction covered $90\%$ of the total water extractable nitrogen. In the precipitation of the extracted proteins, Barnstein method and methanol treatment seemed to be more efficient than other precipitation methods.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.39 no.3
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• pp.177-186
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• 2013

Skin dermal fibroblast is the major collagen-producing cell type in human skin. As aging process continues in human skin, collagen production is reduced and fragmentation is increased, which is initiated by matrix metalloproteinase-1 (MMP-1). This imbalance of collagen homeostasis impairs the structure and function of dermal collagenous extracellular matrix (ECM), thereby promoting skin aging. Cysteine-rich protein 61 (CCN1), a member of the CCN family, negatively regulates collagen homeostasis in primary human skin dermal fibroblast cells. It is known in aging fibroblast cells that elevated CCN1 expression substantially reduces type I procollagen and concurrently increases MMP-1, which initiates fibrillar collagen degradation. And proliferation rate of aging fibroblast cells is reduced compared to the pre-aging fibroblast cells. In this study, we confirmed that the replicative senescence dermal fibroblast cells increased the expression levels of MMP-1 and decreased the production of type I procollagen. Our results also showed that the replicative senescence dermal fibroblast cells increased in the expression of CCN1 and decreased in the proliferation rate. Hydrolyzed Ulva pertusa extracts are the materials to improve photo-aging by reducing the expression of MMP-1 that was increased by ultraviolet and by promoting the synthesis of new collagen from fibroblast cells. In this study, we also investigated the hydrolyzed U. pertusa extract to see whether it inhibits CCN1 protein expression in the senescence fibroblasts. Results showed that the hydrolyzed U. pertusa extract inhibited the expression of MMP-1 and increased the production of type I procollagen in the aging skin fibroblast cells cultured. In addition, the proteins that regulate collagen homeostasis CCN1 expression were greatly reduced. The hydrolyzed U. pertusa extract increased the proliferation rate of the aging fibroblast cells. These results suggest that replicative senescent fibroblast cells may be used in the study of cosmetic ingredients as a model of the natural aging. In conclusion, the hydrolyzed U. pertusa extract can be used in anti-wrinkle functional cosmetic material to improve the natural aging skin care as well as photo-aging.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.38 no.10
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• pp.865-871
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• 2014

This study was conducted with the aim of developing a venturi-type air supply system for a microbubble generator. In order to determine the influence of the varying geometry of the venturi tube on the flow characteristics, a computational fluid dynamics (CFD) simulation was performed using the commercial CFD software ANSYS CFX-15. Furthermore, in order to elucidate the effects of variation in major design dimensions such as the air supply hole size, position of holes, and number of holes on the air supply characteristics, two-phase multiflow CFD analysis was performed. The analysis results showed that the starting point of expansion on the venturi tube with 0.75 is the best hole position and that the air supply hole size and the number of holes are linearly proportional to the amount of air.

• Korean Journal of Environment and Ecology
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• v.11 no.2
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• pp.240-248
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• 1997

기존 연구 문헌의 고찰로 서울시 야생조류상의 현황 및 관리방안은 다음과 같이 결론지을 수 있다. 1. 서울시 야생조류상에 대하여 물새와 산새를 모두 연구한 결과가 아직 발표되지 않아 정확한 현황은 파악하기 어려우나 번식기에는 약 40 여종, 겨울철에는 40여종이 서식하는 것으로 볼 수 있으나, 앞으로 정교하며 지속적인 모니터링이 필요하다. 2. 도시림 뿐만 아니라 주변의 패치를 동시에 고려한 통합적 접근방식(intergrated approach)으로 도시림을 연구하여 관리해야 할 것이다. 3. 도시림의 면적은 도시림에 번식하는 야생조류의 종 수에 큰 영향을 미치는 지배적인 인자로써 도시림이 더 이상 다른 토지이용 형태로 전환되지 않도록 관리하는 것이 가장 기초적인 관리 지침이다. 4. 대면적의 숲이 세분화 될 경우, 여름철새의 번식에 지장이 있을 수 있으므로 대면적 숲의 세분화를 막아야 하며, 가능한 한 생태통로(eco-corridor)로 세분화 된 숲을 연결할 수 있는 기술을 개발해야 할 것이다. 5. 도시림에 생육하는 임목 중 대경급 임목은 나무구멍을 둥지자원으로 이용하는 조류의 중요한 은신처와 둥지이므로 대경급 임목을 대신할 수 있는 인공새집의 설치는 나무구멍을 둥지로 이용하는 조류의 번식 종 수를 높이는 방법이다. 또한, 도시림이 시민에 의해 과도하게 이용될 경우, 관목층이 제거되어 관목층을 둥지자원으로 이용하는 조류의 서식에 불리한 조건을 제공할 수 있으므로 등산로 주변에 관목성 수목을 밀식하여 등산로의 확폭과 파괴를 막아야 할 것이다. 풍부한 먹이 자원을 제공하기 위하여 관목층 보호를 꾀하고 임목의 조림과 무육에 힘써야 할 것이다. 6. 동일한 면적에서 번식 조류 종 수를 높이는 방법은 엽층의 피도량을 늘이는 것이다. 현재 서울의 도시림은 엽층의 분화보다는 우선, 피도량의 증가를 위해 노력해야 하며 후에 엽층의 다양성을 높여 산림의 수직적 구조를 다양하게 만들어야 할 것이다. 7. 들고양이와 들개에 의한 소형 조류의 피해가 늘고 있으므로, 야생조류의 천적인 들고양이와 들개를 구제해야 할 것이며, 이에 앞서 서식실태가 조사되어야 할 것이다. 8. 한각을 비롯한 지천의 하변에 서식, 월동하는 물새류와 서식지에 대한 연구와 관리가 시급히 이루어져야 할 것이다. 9. 산불방지와 도시림 정비의 일환으로 실시해 온 관목층과 낙엽 및 죽은 가지를 제거하는 비과학적인 관행과, 하천유지유량을 높이고 하변 정비 및 산불 방지으 ㅣ한 방편으로 진행된 하변 식생 제거는 전문가와 충분히 제고한 후, 없어져야 할 것이다.

• Transactions of the Korean Society of Mechanical Engineers
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• v.9 no.2
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• pp.150-157
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• 1985

In terms of behavior of fatigue cracks propagated after build-up around the artificial drilled miro-hole, this study has been made of the build-up process of slips and micro cracks, behavior of micro-crack propagation and the definition of fatigue limit under the rotating bending stress with low carbon steel. The results of this study are as follows: (1) The fatigue limit is the repropagating critical stress for the nonpropagating cracks which have grown to some limit around the micro-hole in regard of the magnitude of micro-hole. (2) Behavior of the slips and micro-cracks initiation are occurring simultaneously in front and in rear of micro-hole tips in the view of the rotational direction, regardless of the magnitude of micro-hole. (3) Behavior of fatigue crack propagation is different from magnitude of micro-hole, its behavior is propagation of single crack about respectively large hole, but about respectively small hole, fatigue crack propagated joining phenomena of micro-cracks. (4) The behavior of fatigue fracture is affected by the factor of its defects in the view of magnitude of micro-hole when the diameter of the micro-holes are smaller than 50.mu.m, and this is also affected with the size effect of micro-hole diameter.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.40 no.5
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• pp.275-281
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• 2016

In this study, we investigate the pool boiling enhancement caused by perforated plates on top of a smooth surface. We conduct tests using R-123 at atmospheric pressure. It was shown that perforated plates significantly enhanced the pool boiling of the smooth surface. The reason may be attributed to the increased bubble contact area between the plates. The results showed that the enhancement ratio was dependent on the heat flux. At high heat flux, the enhancement ratio increased as the porosity increased. However, at low heat flux, the enhancement ratio decreased as the porosity increased. For the present investigation, the optimum configuration had a pore diameter of 2.0 mm, pore pitch of $2.5mm{\times}5.0mm$ or $5.0mm{\times}5.0mm$, and a gap width of 0.5 mm, which yielded heat-transfer coefficients that are close to those of GEWA-T. The optimum porosity for R-123 was significantly larger than that of water or ethanol. The reason for this may be the large liquid-to-vapor density ratio along with the small latent heat of vaporization of R-123. The perforated plates yielded smaller boiling hysteresis compared with that of the smooth surface.

• Journal of Korea Multimedia Society
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• v.9 no.9
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• pp.1139-1149
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• 2006

This paper describes a novel and efficient algorithm, which extracts focused objects from still images with low depth-of-field (DOF). The algorithm unfolds into four modules. In the first module, a HOS map, in which the spatial distribution of the high-frequency components is represented, is obtained from an input low DOF image [1]. The second module finds OOI candidate by using characteristics of the HOS. Since it is possible to contain some holes in the region, the third module detects and fills them. In order to obtain an OOI, the last module gets rid of background pixels in the OOI candidate. The experimental results show that the proposed method is highly useful in various applications, such as image indexing for content-based retrieval from huge amounts of image database, image analysis for digital cameras, and video analysis for virtual reality, immersive video system, photo-realistic video scene generation and video indexing system.

• The Korean Journal of Mycology
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• v.22 no.4
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• pp.325-331
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• 1994

White-rot fungus, Schizopora paradoxa did not produce Mn-peroxidase and glucose oxidase without manganese. But, in high concentration of manganese (40 ppm), the activities of both enzymes were higher than those in basal concentration of manganese (11.15 ppm). Unlike the activities of the enzymes, mycelial mass was the same level as the control culture (11.15 ppm manganese) through out the culture period, depending on the concentration of manganese. The same experiments were carried out for the effect of copper and veratryl alcohol added to the culture. The results were not consistent dependent on the concentration of copper and veratryl alcohol, respectively. The involvement of cAMP in the correlative production of MNP and GOX was investigated. In this study, addition of atropine to the culture resulted in a concomitant inhibition of production of MNP and GOX, depending on the concentration of inhibitor added.

• The Korean Journal of Mycology
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• v.36 no.2
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• pp.183-188
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• 2008

A fibrinolytic serine protease was purified from the fruiting bodies of an edible mushroom, Albatrellus confluens. The enzyme had a molecular mass of 30086.41 Da, as measured by MALDI-TOF mass spectrometry. The N-terminal amino acid sequence of the enzyme was Glu-Thr-Val-Thr-Glu-Thr-Thr-Ala -Pro-Trp-Gly-Leu-Ser-Arg-Ile. It displayed optimal activity at $50^{\circ}C$ and within a pH range of $8.0{\sim}10.0$, suggesting that the enzyme is an alkaline protease. The enzyme was stable up to $30^{\circ}C$. The enzyme displayed a strong substrate specificity for the synthetic peptide, N-Suc-Ala-Ala-Pro-Phe pNA. The enzyme activity was completely inhibited by addition of PMSF, indicating that the enzyme is a serine protease. No inhibition was observed following addition of E-64, pepstatin, or EDTA. The activity of the purified enzyme was decreased in the presence $Fe^{2+}$ or $Co^{2+}$, and the enzyme was completely inhibited by addition of $Hg^{2+}$. From these results, we propose that Albatrellus confluens could be used for biofunctional foods development and has potential therapeutic value for the treatment of vascular diseases.