• Korean Journal of Agricultural Science
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• v.40 no.4
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• pp.281-287
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• 2013

In Korea, isolated cultivation has been implemented for 102 genera, including about 250 species, each of which has underwent microscopic inspection, cultivation of bacteria in selective medium, analysis of physiology and biochemistry, enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). The number of isolated microorganisms was 8,307 in the period of 2005-2012, and bulbs and tubers had the greatest diversity of microorganisms, of 5,165 (62.2%), followed by 2,119 (25.0%) sapling, 796 (9.6%) seed, 150 (1.8%) cutting slip, 70 (0.8%) branch graft and 7 (0.1%). The number of cases which were disqualified were 413 (4.97%), after the detection of 47 disease causing species of microorganism. Viruses predominated, with 27 species, followed by 16 fungi, a viroid, a Chromalveolata and 2 further species. Top on the list of detection was Arabis mosaic virus (77 cases), followed by Tobacco rattle virus (70 cases), Lily symptomless virus (46 cases) and Penicillium expansum (46 cases).

• Journal of the Korean Geotechnical Society
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• v.32 no.10
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• pp.67-79
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• 2016

Waveform micropile is an advanced construction method that combined the concept of conventional micropile with jet grouting method. This new form of micropile was developed to improve frictional resistance, which consequently leads to achieving higher bearing capacity and cost efficiency. Two field tests were conducted to examine the field applicability as well as to verify the effects of bearing capacity enhancement. In particular, waveform micropile construction using jet grouting method was performed to evaluate the viability of waveform micropile installation. After testing, the surrounding ground was excavated to check the accomplishment on the shape of waveform micropile. The result showed that waveform micropile can be installed by adjusting the grouting time and pressure. In the loading tests, waveform micropile's bearing capacity increased by 1.4 to 2.3 times depending on their shapes when compared with conventional micropile. Overall results clearly demonstrated that waveform micropile is an enhanced construction method that can improve bearing capacity.

• FLOWER RESEARCH JOURNAL
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• v.17 no.3
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• pp.208-213
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• 2009

Since International Union for the Protection of New Varieties of Plants (UPOV) was established in 1968, the number of joined nations is getting increasing and the regime for variety protection was intensified. Korea joined as the 50th member nation in UPOV. A lot of floricultural plants including rose and chrysanthemum from foreign countries have been applied and registered by Korea Seed and Variety Service (KOSID). Since Korea is one of the importing countries in cut flowers and bulbs, paying royalty is a big burden. Now we are to identify the policy on handling of a mutant from essentially derived varieties (EDV) and relation of a finder (or grower) and breeder by different companies in each country. As a result, the rights are provided not to a finder but to a breeder (breeding company) in most industries. Second, grower's rights are recognized, but in case of the mutant is commercialized, a grower and a breeder should come to a mutual agreement. Third, there is a kind of compensation system for the finder of the mutant.

• Korean Journal of Microbiology
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• v.45 no.3
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• pp.251-256
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• 2009

Leaf samples and bulbs showing characteristic symptoms of virus infection were collected from Gang-won, Chung-nam, and Jeju Province of Korea in 2008-2009. Three viruses, Lily symptomless virus (LSV), Lily mottle virus (LMoV), and Cucumber mosaic virus (CMV) were detected by RT-PCR. Virus-infected plant samples were identified; 12 plants with LSV, 20 plants with LMoV, and 1 plant with CMV. Of the twelve LSV infected samples, seven samples were found to be mix-infected with LMoV and LSV. Symptoms of LMoV and LSV mixed infection were fairly severe, like as vein clearing, leaf curling, leaf mottling, leaf mosaic, and yellow streaking. Mixed infection with LMoV and LSV was also found in lily bulbs which have been stored under unfavorable environmental conditions. LMoV predominated in our tests, whereas spread of Lilyvirus X (LVX) was not found. The nucleotide sequences of coat protein (CP) region of seven isolates (4 LMoV, 2 LSV, and 1 CMV) were compared with the corresponding regions of LMoV (AJ564636), LSV (AJ516059) and CMV(AJ296154). The nucleotide sequence homologies between reference viruses and seven isolates were 95-99%. Complete sequencing of seven isolates is necessary to obtain more information on the molecular characteristics of these viruses as well as to increase sensitivity and rapidity of viral detection.

• Journal of Bio-Environment Control
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• v.31 no.2
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• pp.133-141
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• 2022

Bulbophyllum auricomum Lindl. is a rare orchid and has flowers with an attractive fragrance. The present study investigated the tissue culture method for micropropagation. Capsules derived from artificial self-pollination were obtained for the best seed germination in MS basal medium. Plant growth regulators (1.0 mg·L^-1 of BAP and 2.0 mg·L^-1 of NAA) were affected by callus induction from subcultured pseudobulb explants. For the callus subculture, different natural plant extracts were tested in 11 treatment media. Among them, MS medium with 150 mL·L^-1 of coconut water was generally effective in fresh weight (1.75 ± 0.08) and (3.01 ± 0.20) of callus proliferation and PLBs induction at 1 and 2 months, respectively, followed by an MS combination of 30 g·L^-1 of banana and 20 g·L^-1 of potato extract. The results of a comparative study of different MS mediums containing plant growth regulators with a natural extract combination and MS medium supplemented with natural extract only showed that MS medium supplemented with a combination of natural extracts (150 mL·L^-1 of coconut water) and plant growth regulators (2.0 mg·L^-1 of BAP and 1.0 mg·L^-1 of NAA) obtained the highest shoot regeneration (3.37 ± 0.17) and (6.41 ± 0.68) after 1 month and 2 months of culturing, respectively.

• Korean Chemical Engineering Research
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• v.48 no.4
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• pp.519-533
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• 2010

Activity oefficients and solubilities of L-Alanine in aqueous solutions containing each of four electrolytes(NaCl, KCl, $NaNO_3$ and $KNO_3$) were measured at 298.15 K. The measurements of activity coefficients were carried out in the electrochemical cell coupled with two ion-selective electrodes(cation and anion), and the solubilities were measured by the gravimetric analysis of saturated solutions in equilibrium with the solid phase of L-alanine. To model the activity coefficients and solubilities of amino acid in the amino acid/electrolyte aqueous solutions, thermodynamic relations of the residual Helmholtz free energy in the amino acid/electrolyte aqueous solutions were developed based on the perturbed-chain statistical associating fluid theory(PC-SAFT) combined with the primitive mean spherical approximation(primitive-MSA). In the present model, it is assumed that the zwitterions of L-alanine are associated with each other and cross-associated with water molecules, and also cross-associated with the cation and anion dissociated from an electrolyte(inorganic salt). The activity coefficients and solubilities of L-Alanine calculated from the theoretical model proposed in this work are found to be well agreeable with experimental data.

• The Journal of the Convergence on Culture Technology
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• v.4 no.4
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• pp.325-329
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• 2018

There are two kinds of calla belonging to the Zantedeschia spp. depending on the growing condition. Between them, various colored calla with flower shape and flower color are very popular as cut flowers. However, colored calla is very low in natural reproduction rate, and it takes a long time to obtain a flowering bulb. So it is urgent to establish a large breeding system of good varieties. In this study, various plant growth hormone treatments were carried out to improve the growth of the plants in calla. Root formation was the highest at 81.5 % in basal MS medium, and the formation of shoot and multiple shoots were the highest at 100.0 % and 36.4 % in the combined treatment of BA 2.0 mg/L and IBA 1.0 mg/L, respectively. In fresh weight, the highest growth rate was observed with 11.2 times increase in BA 2.0 mg/L single treatment. Auxin and cytokinin mixed treatment were widely used in previous studies and positive effects on the growth of calla plants will help to establish the micro-propagation system.

• Journal of the Korean Geotechnical Society
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• v.26 no.6
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• pp.5-19
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• 2010

The purpose of this study is to figure out the effect of pressurized grouting on the pullout resistance and the group effect of the compression ground anchor by performing pilot-scale chamber tests and field tests. The laboratory tests are carried out for 3-types of soils which are abundant in the Korean peninsular. Experimental results showed that the enlargement of anchor diameters estimated from the cavity expansion theory matches reasonable well with that obtained from experiments. Moreover, the required injection time as a function of the coefficient of permeability of each soil type was proposed. A series of in-situ anchor pullout tests were also performed to experimentally figure out the effect of pressurized grouting on the pullout resistance. Experimental results also showed that the effect of the pressurized grouting is more prominent in a softer ground with smaller SPT-N value in all of the following three aspects: increase in anchor diameter; pullout resistance; and surface roughness. The pressurized grouting effect in comparison with gravitational grouting was found to be almost nil if the SPT-N value is more than 50. Based on experimental results, a new equation to estimate the pullout resistance as a function of the SPT-N value was proposed. And based on in-situ group anchor pullout tests results, a new group effect equation was proposed which might be applicable to decomposed residual soils which are abundant in the Korean peninsular.

• Journal of Plant Biotechnology
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• v.37 no.1
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• pp.102-109
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• 2010

Fructan has been found to accumulate in various tissues during periods when light levels increased carbon fixation where low temperatures reduced growth rates while photosynthesis continued. In this study, we have cloned 1-sucrose:sucrose fructosyl transferase(1-sst) and 1-fructan: fructan fructosyl transferase (1-fft, a key enzyme for the synthesis of fuctan) from Jerusalem Artichoke (Helianthus tuberosus L.). The recombinant vector with 1-sst and 1-fft has been constructed under the control of 35S promoter of KJGV-B2 vector and transgenic plants obtained by Agrobacterium tumefaciens LBA4404. PCR analysis carried out on the putative transgenic plants for amplification of the coding region of specific gene (1-sst, 1-fft), and HPT genes. Transgenic lines carrying of 1-sst and 1-fft were confirmed for integration into the rice genome using Southern blot hybridization and RT-PCR. The transgenic plants in $T_2$ generation were selected and expression pattern analysis revealed that 1-sst and 1-fft were stable. This analysis confirmed the presence of low-molecular-weight fructan in the seedling of the transgenic rices. Therefore, cold tolerance and carbohydrate metabolism will be possible to develop resistant plants using the transgenic rice.

• Korean Journal of Medicinal Crop Science
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• v.2 no.1
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• pp.14-19
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• 1994

Elephant food(Amorphophallus konjac K.) have been utilized its tubers in workedmaterials for a health and diet food. The author supposed that it was increased the area of cultivation and demand. This experiments were conducted to select the proper covering material during winter in order to increase yield of tubers and decrease input by 2 year's continuous cultivation, also to verified ability of seed germination and to measured efficiency of photosynthesis of plant. The proper covering materials for wintering were rice straw and rice hull. These materials were covered at 5 cm thick and at field was promoted according to emergence appearing after winter. The yields were 5,790kg /10a at 4,730kg /10a, respectively. Yield increase was 120% and 80% than that of control. The seeds collected at August 22 were germinated about 84 percent, and it was not necessary to treatment of low temperature or germination-accelerated chemicals. The widest leaf area was ranged $1,218-1,438cm^2$ at October 20 and was varied. The efficiency of photosynthesis was highest at 65-95 days after leaf emergence. The line of broad leaf and high photosynthetic efficiency per unit area was greater compare with yield. Therefore, it was supposed that these characteristics will use a marker for selection for high-yielding lines.