• The Korean Journal of Oral and Maxillofacial Pathology
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• v.42 no.5
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• pp.111-118
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• 2018

Nicotine of tobacco component has a controversial impact in the clinical outcome of dental implants. Although numerous nicotine effects on bone healing around implants have been presented, it is rarely reported in vitro study about normal human osteoblast(NHost) from oral and maxillofacial area at the surface of implants. The purpose of the present study was to evaluate the effect of nicotine on the proliferation and differentiation response of NHost to plasmatic and salivary levels of nicotine reported in smokers at the surface of screw-type plasma-sprayed titanium implants. NHosts were seeded on the surface of titanium implants and cultured for 21 days in ${\alpha}-MEM$ supplemented with 10% FBS, 50mg/ml ascorbic acid, 5mM ${\beta}$-glycerophosphate and 100nM dexamethasone. Seeded implants were exposed to various nicotine concentration(0.05-0.5mg/ml) from 1 to 21 days, and characterized for cell morphology, proliferation, differentiation, alkaline phosphatase(ALP) activity and ionized calcium concentration(Cai) of medium. Continuous exposure to higher nicotine concentration(above 0.3mg/ml) induced a dose- and time-dependent vacuolation of the cytoplasm, and a tendency to detach from the implant surface. 0.05mg/ml(lower nicotine concentration) did not cause significant effects in the cell proliferation and ALP activity. 0.1-0.2mg/ml caused evident dose-dependent effects in increased cell proliferation, ALP activity and earlier onset of matrix mineralization at levels up to 0.2mg/ml, while a dose-dependent inhibitory effect at 0.3-0.5mg/ml. Cai concentration of control group was decreased at 14 days. Increased Cai concentration at 0.1-0.2mg/ml, decreased Cai concentration at 0.3mg/ml and no change at 0.5mg/ml during the culture period were seen. It suggested that nicotine concentration could paly an role in modulating NHost activity as a contributing factor associated with proliferation and differentiation of NHost at the surface of implants.

• The Journal of Korean Academy of Prosthodontics
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• v.61 no.3
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• pp.234-244
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• 2023

Tooth wear refers to the loss of dental hard tissue caused by various physiological and pathological causes, and excessive pathological wear can cause complications such as pathological changes in dimensions, occlusal disharmony, loss of function, and aesthetic problems. The cause of tooth wear can be caused by attrition, abrasion, corrosion and abfraction, and it is known to act in a multifactorial etiology in interocclusal activity. In patients with excessive pathological wear, it is important to determine whether or not the vertical dimension of occlusion is reduced, and complete oral rehabilitation should be achieved with the adaptation of the neuromuscular and temporomandibular joint through accurate diagnosis and analysis. The patient in this case was a 63-year-old male patient, who presented discomfort to cold beverage due to severe tooth wear. After analysis of the patient's vertical dimension of occlusion, a full mouth rehabilitation was performed with increasing vertical dimension of occlusion. The goal of treatment was to improve the occlusal plane with the equal-intensity contact of all teeth, harmonious anterior guidance and immediate disclusion of all posterior contacts. After rehabilitation, the patient was satisfied with function and esthetic appearance.

• Journal of Chest Surgery
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• v.37 no.3
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• pp.279-281
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• 2004

Behest's disease is manifested as ulcers present in oral cavity, on external genital organ, orbital area, and skin. This disease could also cause other symptoms by invading the cardiovascular system, respiratory system, gastrointestinal system, central nervous system, and urogenital system. It is very rare for Behcet's disease to be accompanied by carotid artery aneurysm and intestinal perforation. We report a patient with Behcet's disease, who is diagnosed with symptoms and pathological findings as haying carotid artery aneurysm and intestinal perforation at the same time. The patient underwent operation for the aneurysm and the intestinal perforation.

• The Journal of the Korean dental association
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• v.25 no.6 s.217
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• pp.541-548
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• 1987

골수염은 조기에 적절한 치료를 하지않으면 중대한 후유증이 초래되므로 신속한 진단과 치료가 요구된다. 골수염을 진단하기 위해서는 구강검진, 방사선검사, 병리조직학적 검사, 혈액 및 세균배양검사와 골스캔등이 필요하다. 골수염의 감염과정은 골에 파괴적 및 재생적 변화의 양자를 모두 일으키지만 X선사진소견에서는 임상증상이 발현된 후 약 10내지 14일이 경과되어야만 골변화를 관찰할 수 있으며 그 이전에는 대체로 비특이적 연조직의 종창만을 보인단. 반면에 골스캔을 이용하면 급성염증기의 골질환을 감견하기가 용이하다. 골스캔제의 국소골섭취의 증가는 골의 어떤 부위에서 대사능이나 혈류가 증가된 경우에 일어나기 때문에, 실제로 임상증상이 발현된 후 24시간정도의 조기에 골스캔상은 양성반응을 보인다. 즉 골의 국소적 병변은 어느정도 신생골 형성과 골파괴가 같이 수반되어있고 이러한 신생골은 골스캔제와 친화력이 강하므로 골형성반응을 야기하는 어떠한 병변이라도 골스캔에 의하여 발견될 수 있다. 임상적으로 골수염에 대하여 골스캔을 시행하는 경우는 골수염의 조기진단, 골수염과 봉와직염과의 감별진단, 만성 공수염의 악화여부 및 치료에 대한 반응을 monitoring하기 위해서 시행된다.

• Journal of the korean veterinary medical association
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• v.28 no.8
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• pp.470-475
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• 1992

개업수의사로서 실천할 수 있는 예방의학 1. 효과적인 예방주사접종과 정확한 주사간격(예방주사접종일이 되면 축주에게 전화나 엽서로 시기가 되었음을 알려준다.) 2. 영양교육과 grooming 상담을 통한 건강상태 개선과 관계되는 처방약품 또는 양질의 용품판매 3. 비상/상비약품 필요성 인식과 판매, 건강유지를 위한 영양제의 계속적인 필요성 강조로 축주의 영양관리에 대한 인식개선 4. 1년에 2회, 분변검사를 통한 정기적인 기생충 r제와 일단 감염시 2회 치료와 효과확인 5. 구강검사를 통한 제질병의 발견과 치아검사의 필요성강조 및 치아건강 관련제품 사용권장 6. 각 품종별 특이한 질병의 설명과 예방을 한 검사(X-ray, 병리검사)의 추천과 결과에 따라 진행방지 방법이나 수술/치료 권유 7. 적극적인 진료자세-예)종양의 보다 세밀한 검사와 치료로 전이예방

• The Journal of the Korean dental association
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• v.9 no.6
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• pp.311-315
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• 1971

The author has studied histopathologically on the 24 cases of precancerous lesions 14 cases of benigh tumors and 3 cases of odontogenic cysts transformed to malignancy. The results are as follows: 1. On the 4 cases of leukoplakias, could observed precancerous changes such as hyperkeratosis, dyskeratosis and indistinct basement membrane. 2. The proliferative epithelium in the chronic inflammatory gingivitis, revealed precancerous conditions such as loss of polarity, mitotic figures with cellular pleomorphism and dyskeratosis. 3. The proliferative epithelial islands in the 2 cases of epitheliated dental granulomas could observe the cellualr malignancy. 4. Oral tuberculous lesions can become precancerous lesions inducing cancerous proliferation. 5. Oral benign tumors such as fibromas, salivary mixed tumors and ameloblastomas can be regarded as precancerous condition that the more recurrent they become the more likely they may be to transforme malignancy. 6. The proliferation of covering epithelium in the odontogenic cysts needs attention as its possible transformation to a precancerous condition.

• The Journal of the Korean dental association
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• v.38 no.11 s.378
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• pp.1075-1081
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• 2000

• Proceedings of the Korean Society of Computer Information Conference
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• 2021.07a
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• pp.359-360
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• 2021

디지털 헬스케어는 헬스케어 산업과 ICT 기술이 융합되어 개인 건강 및 질환을 관리하는 분야로서 소비자 중심의 서비스 제공을 위해 관련 산업이 크게 발전하고 있다. 최근 감염병 시대에 디지털 건강기술을 활용한 비대면 진료에 대한 요구가 급격히 증가하고 있다. 본 연구에서 제안하는 디지털 헬스케어 플랫폼은 비대면 치아 진단 및 치료 견적 플랫폼으로서 시간적·공간적 제약 없이 충치 환자에게 편리한 구강검진이 가능하게 하고, 직접 치과를 방문하지 않아도 치과 전문의에게 본인의 치아 상태를 검진받을 수 있다. 또한, 여러 치과 전문의에게 본인의 치아 치료 견적을 받고 가격을 비교하여 본인이 원하는 적절한 가격과 방법으로 치료하여 비용을 절약할 수 있도록 한다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.26 no.4
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• pp.337-344
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• 2000

Germ-line mutations at DNA repair loci confer susceptibility to colon cancer in hereditary non-polypopsis colorectal cancer. Somatic loss of DNA mismatch repair gene has been reported in a large variety of other tumor types. Replication errors(RERs) judged by microsatellite instability(MSI) and its associated mutations have been recognized as an important mechanism in various tumor types. To investigate associations between MSI and oral squamous cell carcinoma, the frequency of MSI using 12 microsatellite markers were analyzed for the series of oral tumors. Of 17 tumors, 8 cases(47%) did not show instability at any of the 12 loci; 5(29%) showed instability at $2{\sim}3$ loci; and 4(24%) showed instability above 4 loci. The 4 cases showing widespread MSI did not differ from those without evidence of instability in terms of age at diagnosis, degree of differentiation, metastasis to lymph node, tumor location or the presence of mutations in the p53 tumor suppressor gene. DCC and D17S 796 were the most frequently detected in MSI analysis. There were no correlation between smoking and MSI frequency, instead, smoking was suggested to increase the mutation rate of p53 and development of oral carcinomas.

• The Korean Journal of Oral and Maxillofacial Pathology
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• v.41 no.3
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• pp.121-129
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• 2017

Coffee (Coffea spp.) is one of the most important agricultural commodities, being widely consumed in the world. Various beneficial health effects of coffee have been extensively investigated, but data on habitual coffee consumption and its bio-physiological effect have not been clearly explained as well as it is not proved the cause and effect between drinking coffee and its bio-physiological reactions. We made the dialyzed coffee extract (DCE), which is absorbable through gastrointestinal tract, in order to elucidate the cellular effect of whole small coffee molecules. RAW 264.7 cells, a murine macrophage lineage, were directly treated with DCE, i.e., DCE-2.5 (equivalent to 2.5 cups of coffee a day), DCE-5, and DCE-10, for 12 hours, and their protein extracts were examined by immunoprecipitation high performance liquid chromatography (IP-HPLC). RAW 264.7 cells differently expressed the inflammation-related proteins depending on the doses of DCE. RAW 264.7 cells treated with DCE showed marked increase of cathepsin C, cathepsin G, CD20, CD28, CD31, CD68, indicating the activation of innate immunity. Particularly, the macrophage biomarkers, cathepsin G, cathepsin C, CD31, and CD68 were markedly increased after DCE-5 and DCE-10 treatments, and the lymphocyte biomarkers, CD20 and CD28 were consistently increased and became marked after DCE-10 treatment. On the other hand, RAW 264.7 cells treated with DCE showed consistent increase of IL-10, an anti-inflammatory factor, but gradual decreases of different pro-inflammatory proteins including $TNF{\alpha}$, COX-2, lysozyme, MMP-2, and MMP-3. In particular, the cellular signaling of inflammation was gradually mitigated by the reduction of $TNF{\alpha}$, COX-2, IL-12, and M-CSF, and also the matrix inflammatory reaction was reduced by marked deceases of MMP-2, MMP-3, and lysozyme. These anti-inflammatory expressions were consistently found until DCE-10 treatment. Therefore, it is presumed that DCE may have dynamic effects of innate immunity activation and pro-inflammation suppression on RAW264.7 cells simultaneously. These effects were consistently found in the highest dose of coffee, DCE-10 (equivalent to 10 cups of coffee a day in man), that might imply the small coffee molecules were accumulated in RAW 264.7 cells after DCE-10 treatment and produce synergistic cytokine effects for innate immunity activation and anti-inflammatory reaction concurrently.