• Title/Summary/Keyword: 광노화

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Effect of Green Tea Catechins on the Expression and Activity of MMPs and Type I Procollagen Synthesis in Human Dermal Fibroblasts (사람 섬유아세포에서 녹차 카테킨이 노화 인자인 MMP와 type 1 Procollagen 발현에 미치는 영향)

  • Shin, Hyun-Jung;Kim, Su-Nam;Kim, Jung-Ki;Lee, Byeong-Gon;Chang, Ih-Seoup
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.32 no.2 s.57
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    • pp.117-121
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    • 2006
  • Although many studies have been performed to elucidate the molecular consequence of factors that regulate skin aging, little is known about the effect of green tea catechins except EGCG. The matrix metalloproteinase (MMP), can degrade matrix proteins and results in a collagen deficiency in photodamaged skin, are known to play an important role in photoaging. This study, investigated the effects of green tea catechins on the UVA-induced MMP-1 expression, activity of MMP-2 and synthesis of type I procollagen in human dermal fibroblasts. We examined eight catechins that naturally exist in green tea leaves and compared their efficacies among them. Most of catechins inhibited the expression of MMP-1 in dose dependent manner, and the levels were reduced, especially, 57.4 and 68.2% by treatment with $1{\mu}M$ of epigallocatechin-3-gallate (EGCG) and gallocatechin-3-gallate (GCG), respectively. Also, catechins significantly suppressed the activities of MMP-2. Catechins also induced the expression of type I procollagen, however, they acted only at the concentration below $1{\mu}M$ interestingly. Furthermore, when EGCG:GCG:ECG had the ratio of 0.5:1.5:.1.3, they presented the most effective on procollagen synthesis. Therefore, we concluded that catechins significantly inhibited MMPs and induced collagen synthesis. Taken together, all these results suggested that green tea catechins might be good natural materials act as an anti-photoaging and a skin-aging improving agent.

Power variation of Solar cell according to Electrolyte (전해질에 따른 Solar Cell의 출력 변화)

  • Shin, Jun-Oh;Jung, Tae-Hee;Kim, Tae-Bum;Won, Chang-Sub;Ji, Yang-Geun;Kong, Ji-Hyun;Kang, Gi-Hwan;Ahan, Hyung-Keun;Han, Deuk-Young
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.06a
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    • pp.471-472
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    • 2009
  • 최근 태양전지의 내구성과 관련하여 모듈의 노화에 따른 효율 저하 현상이 대두되고 있다. 노화현상원인 중의 하나인 Hot spot 혹은 염분에 의한 백화현상으로 Solar Cell 과 EVA Sheet간 막들뜸 현상이 발생하며 그로 인한 공기 및 수분에 의해 금속 산화가 이뤄진다. 본 연구에서는 특히 전해질의 농도에 따른 금속의 산화와 그 출력에 대해 다룸으로써 태양광 발전 설치 지역 조건에 따른 Module의 내구성을 검토해 보았다. 산성비의 대부분을 구성하고 있는 황산과 질산의 PH를 0.1 간격으로 Split하여 농도의 변화에 의한 금속 부식과 그에 따른 전기적 저항 및 출력변화를 통해 태양광 Module 최적의 설치 지역조건을 제시하였다.

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Effects of Ultraviolet Radiation on the Skin (자외선에 의한 피부반응)

  • Youn, Jail-Il
    • Journal of Radiation Protection and Research
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    • v.20 no.3
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    • pp.181-186
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    • 1995
  • UV irradiation causes a variety of biologic effects on the skin. These effects can be devided to acute reactuons and chronic reacxtions by duration of UV irradiation. Acute reactions are erythema reaction, pigment reactions and changes in epidermal thickness. Among them erythema reaction is most common and conspicuous acute effects of the skin. Upon exposure to sun or artificial UV soures, a faint redness response of skin may begin. Larger exposure causes sunburn reaction which is exaggerated erythema reactionassociated with pain, swelling, vesicle and dulla. Extent and time course of erythema reaction depend upon several factors including wavelength and dose of UVR, skin conditions likeas skin type, site, color, temperature, humidity and environmental factors. Evaluation of erythema erythema induced by UV irradiation is difficult to quantify. Degree of redness of skin are usually estimated by subjective visual evaluation. The lowest exposure dose required to protuce erythema is called minimal erythema dose (mod). Repeated exposures of UVR result in photaging skin. In this condition we can see wrinkling, skin atrophy, dilated blood vessels and keratoses. In sensitive persons photocarcinogenesis is can Be developed on exposed area of skin. Recently skin canser is increasing now in our country. An effective public education and photopreventive method must be developed.

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Inhibitory effect of Aralia elata ethanol extract against skin damage in UVB-exposed human keratinocytes and human dermal fibroblasts (두릅순 에탄올 추출물의 인간유래 피부각질형성세포와 피부섬유아세포에서의 자외선에 의한 광노화 억제효과)

  • Yang, Jiwon;Kwak, Chungshil
    • Journal of Nutrition and Health
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    • v.49 no.6
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    • pp.429-436
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    • 2016
  • Purpose: Solar ultraviolet (UV) radiation causes inflammation and matrix metalloproteinase (MMP) overexpression and extracellular matrix depletion, leading to skin photoaging such as wrinkle formation, dryness, and sagging. Activation of MMP is influenced by various molecules such as reactive oxygen species (ROS), proinflammatory cytokines, and transient receptor potential vanilloid type (TRPV)-1, which are increased in UV-irradiated skin cells. Aralia elata (AE) ethanolic extract was reported to inhibit ROS generation caused by UVB-irradiation in keratinocytes. In this study, we investigated the photoprotective effect of AE ethanolic extract on UVB-irradiated human keratinocytes (HaCaT) and human dermal fibroblasts (HDF). Methods: AE was freeze-dried, extracted in 70% ethanol, and concentrated. Skin cells were treated with AE extract for 24 h and then exposed to UVB ($55mJ/cm^2$). After 48 h of incubation, proinflammatory cytokines, MMP-1, type-1 procollagen, and TRPV-1 levels were measured by ELISA or Western blotting. Results: Treatment with AE extract ($100{\mu}g/mL$) significantly inhibited UVB-induced IL-6, IL-8, and $PGE_2$ production in HaCaT by 25.6%, 5.3%, and 70.2%, respectively, and also inhibited elevation of MMP-1 and TRPV-1 caused by UVB irradiation by 20.0% and 41.9%, respectively (p < 0.05). In HDF, AE extract treatment significantly inhibited both elevation of MMP-1 and reduction of type-1 procollagen caused by UVB irradiation (p < 0.05). In addition, type-1 procollagen was elevated by AE extract treatment in normal HDFs (p < 0.05). Conclusion: AE 70% ethanol extract has photoprotective ability via reduction of proinflammatory mediators, TRPV-1 and MMP-1 production, and elevation of collagen synthesis. Our findings suggest that AE extract might be a good natural material to protect against UVB-induced premature skin aging.

Effect of Germination Temperature and Light Quality on Germination of Pretreated Gourd Seeds (발아온도 및 발아중 광질 처리가 전처리된 박 종자의 발아에 미치는 영향)

  • 강신윤;강진호;전병삼;최영환;이상우
    • Journal of Life Science
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    • v.13 no.1
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    • pp.9-14
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    • 2003
  • Seed germinability might be highly related to seedling establishment. The experiments were carried out to evaluate the effect of presowing treatments of aging, priming, $GA_3$ , prechilling and water imbibition, different temperatures and light quality during germination on germination of gourd (Lagenaria siceraria Standl.) seeds. Aging treatment with different temperatures and periods was done by the accelerated aging method. Priming using KNO$_3$ and $GA_3$ treatment for 24 hours were done at 100 mM and at 0.01 mM before a week prechilling. The germination tests using a week prechilled seeds were done at 10, 20 and $30^{\circ}C$ on the condition of darkness, and also with blue, red, far-red light and darkness as light quality treatments. FR-yongjadaemok and FR-kunghap were used as test cultivars and their daily germination rates were measured at treatment levels. Germination rates were reduced in the seeds aged at $45^{\circ}C$ for 6 days. Prechilling showed the highest and fastest rate among the four presowing treatments but the two other presowing treatments had similar or less rates than water imbibition. Prechilling done before sowing enhanced the low vigor seeds, and accelerated the germination of cv. FR-kunghap at 20 to $30^{\circ}C$ and with blue, red light or darkness during germination although far-red light inhibited their germination.

Inhibitory Activity of Blueberries on UVB-induced Oxidative Stress and Matrix Metalloproteinase Expression in Human Skin Fibroblasts (인간피부 섬유아세포에서 UVB 유도된 산화 스트레스와 기질금속단백질가수분해효소 발현에 블루베리의 저해능)

  • Jang, Young Ah;Kim, Se Gie
    • Journal of Life Science
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    • v.29 no.12
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    • pp.1321-1328
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    • 2019
  • Intermediate-wavelength solar radiation, also known as ultraviolet B (UVB: 290-320 nm) radiation, may cause premature aging and oxidative damage-dependent skin cancer in humans. UVB-induced formation of reactive oxygen species (ROS)-often a consequence of excessive exposure to these rays-could activate matrix metalloproteinases (MMPs) such as MMP-1 and MMP-3. These enzymes break down type I collagen in human fibroblasts. In this study, we assessed the antioxidant and anti-aging effects of ethyl acetate extract of blueberry (EEB). An antioxidant test in blueberries evaluated ROS production using CCD-986sk cells and DPPH assay. In order to evaluate the anti-wrinkle efficacy of blueberries, the MMP-1 production and type 1 procollagen synthesis evaluated and the expression of MMP 1, 3 were tested through Western blot and RT- PCR. EEB exhibited 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reduced the production of UVB-induced ROS. Also, EEB inhibited UVB-induced processes associated with photoaging and skin cancer, such as reduction in procollagen production and increase in MMP-1 production. More precisely, EEB (50 ㎍/ml) markedly suppressed mRNA and protein levels of MMP-1 and -3. The anti-aging effects are attributable to the antioxidant activity of EEB. These findings indicate that EEB has a protective effect against UVB-induced aging in human fibroblast cells by regulating the levels of type-1 procollagen, MMP-1, and MMP-3.

The Protective Effect of Spirulina-derived Phycocyanin on Dermal Fibroblasts Induced by UV Rays (자외선으로 손상을 유도한 피부섬유아세포에서 스피룰리나 유래 피코시아닌의 보호 효과)

  • Yang, Jae Chan
    • Journal of the Korean Applied Science and Technology
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    • v.38 no.5
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    • pp.1249-1254
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    • 2021
  • UV induce oxidative stress and increase matrix metalloproteinase (MMP) expression, resulting in skin aging. Thus, preventing skin damage from ultraviolet B (UVB)-induced skin damage can attenuate skin aging. Spirulina is comprised of prokaryotes a powerful antioxidant. This study aimed to investigate the photoprotective effects of spirulina-derived phycocyanin (PC) against UVB radiation using human skin fibroblast. As a results, PC showed no toxicity at concentrations of 5-40 ㎍/mL in terms of fibroblast viability. Survival rate of UVB-irradiated fibroblast incresased to 73.5% from 50.5% with PC treatment. UVB treatment increased MMP-1 and MMP-9 expression whereas PC treatment decreased it. The results indicate that PC might reduce or prevent skin aging by reducing UVB irradiation-induced skin wrinkles and free radicals.

태양광 발전용 축전지의 연구 개발 현황

  • 김인곤;강홍열
    • 전기의세계
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    • v.39 no.10
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    • pp.28-36
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    • 1990
  • 태양광발전 시스템에서 축전지는 일조시간에 태양 전지에 의해 충전된 전력을 일몰 후와 같이 태양전지로 부터의 출력이 없을때나 흐린날, 우천시와 같이 태양 전지의 출력이 부족할 때 방전하여 부하에 전력을 공급하게 된다. 이와같이 축전지는 태양 광발전 시스템이라는 특별한 용도에 사용되기 때문에 일반적인 용도와는 달리 매우 가혹한 사용 조건하에서 가동된다. 즉, 축전지의 충전량은 기후 변화에 의해서 좌우되므로 과충전, 부족충전, 과방전이 진행되면 축전지의 노화 즉, 용량 감소는 급격히 일어나게 된다. 물론 최적의 충전 상태를 유지하기 위해서 충전기를 개량하거나, 부조일을 감안하면 축전지의 용량을 설계하거나, 디젤 보조발전기를 사용하여 일사량이 적은 경우에 충전 전기량을 보충하는 등 여러가지 개선책이 있지만, 불규칙적이며 가혹한 사용조건은 자연 현상에 의한 것이므로 근본적인 해결책은 될 수가 없다.

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Development of Anti-Aging Products (Anti-Wrinkle) like Epidermal Growth Factor(EGF) Materials using Supercritical Heat-Treated Extract Radish (초임계 열처리된 무 성분을 이용한 상피세포성장인자(EGF) 유사소재 개발 및 광노화(주름개선) 효과)

  • Kim, Hyun Kyoung
    • The Journal of the Convergence on Culture Technology
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    • v.4 no.3
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    • pp.197-207
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    • 2018
  • The radish skin and radish greens (mucheong) are an edible part of the radish. But they are removed before eating the radish and used as a byproduct or an animal feed material because of their tough and rough texture. This study was conducted to investigate the effect of supercritical heat-treated radish-extract on UV-induced Hos: HRM-2 wrinkled mouse animal model on anti-aging wrinkles. Supercritical heat-treated radish-extract was applied on the back of seven-weeks old HRM-2 mice. The effect of HRE on skin thickness, elasticity and wrinkle formation of the mice was observed by using UVB lamp to induce melanogenesis and wrinkle formation. As the result, increased depth of wrinkles was observed in the negative control group in comparison to the normal group. In contrast, decreased depth of wrinkles was observed in the radish-extract-free group compared to the negative control group. In the study of the effect of radish-extract on wrinkle-formation related gene expression and protein what protein expression, MMP-2 and MMP-2 gene expression significantly increased in the negative control group compared to the normal group. The gene expression reduced independence to the mass of radish-extract treated. Similar to quantitative results of mRNA expression, the expression of MMP-2 protein increased as a result of UVB-irradiation. The MMP-2 expression was inhibited in dependence to the mass of radish-extract treated. In conclusion, the supercritical heat-treated radish-extract has an effect on improving skin wrinkles not only when it is applied to the skin but also when orally ingested. Thus, it can be effectively used as a composition to health functional products. Thereafter, we can also conclude that radish, a food that does not show any side-effects even upon long-term intake, can reduce wrinkle formation as well as improve skin elasticity when taken regularly for a long period.

Intelligent Prediction System for Diagnosis of Agricultural Photovoltaic Power Generation (영농형 태양광 발전의 진단을 위한 지능형 예측 시스템)

  • Jung, Seol-Ryung;Park, Kyoung-Wook;Lee, Sung-Keun
    • The Journal of the Korea institute of electronic communication sciences
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    • v.16 no.5
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    • pp.859-866
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    • 2021
  • Agricultural Photovoltaic power generation is a new model that installs solar power generation facilities on top of farmland. Through this, it is possible to increase farm household income by producing crops and electricity at the same time. Recently, various attempts have been made to utilize agricultural solar power generation. Agricultural photovoltaic power generation has a disadvantage in that maintenance is relatively difficult because it is installed on a relatively high structure unlike conventional photovoltaic power generation. To solve these problems, intelligent and efficient operation and diagnostic functions are required. In this paper, we discuss the design and implementation of a prediction and diagnosis system to collect and store the power output of agricultural solar power generation facilities and implement an intelligent prediction model. The proposed system predicts the amount of power generation based on the amount of solar power generation and environmental sensor data, determines whether there is an abnormality in the facility, calculates the aging degree of the facility and provides it to the user.