• Title/Summary/Keyword: 과학 과정 기술

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Determination of Di-(2-Ethylhexyl) Adipate Migrated from Polyvinyl Chloride Wrap Film into Various Foodstuffs and Dishes Depending on Exposure Conditions (염화비닐 랩 필름으로 포장된 다양한 식품 및 요리류의 노출 조건에 따른 di-(2-ethylhexyl) adipate의 이행량 분석)

  • Lee, Young-Ho;Gyoung, Young-Soo;Lee, Keun-Taik
    • Korean Journal of Food Science and Technology
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    • v.34 no.6
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    • pp.969-976
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    • 2002
  • The migrating level of di-(2-ethylhexyl) adipate (DEHA) in PVC wrap to various foodstuffs dishes was determined using acetone/n-hexane as an extracting solvent. The recovery ratio of DEHA from various foodstuffs ranged from 81.3 to 91.2%. During storage at $10\;or\;20^{\circ}C$ for up to 3 days, highest migration of 22.9 mg/kg occurred with pork belly, an indication that increases in fat content, storage temperature, and storage time result in increasing DEHA migration. DEHA concentrations of various delivered dishes ranged between 6.9 and 29.8 mg/kg, and highest migration was observed in Chambbong, which had the highest fat content among samples. After microwave-reheating, which resulted in a tight contact between film and food samples, DEHA was not detected in rice and potato, whereas up to 158.8 mg/kg was detected in pizza. When the wrap film was not contacted with the surface of food, the migration was lowered. Migration levels of DEHA from PVC wrap film into samples under various exposure conditions often exceeded the limit value of $3\;mg/dm^2\;and/or\;18\;mg/kg$ set by the European Union.

Selection of Lines Resistant to Phytophthora capsici from Capsicum chinense, Korean Land Races and Resistant Genetic Resources of Pepper (Capsicum chinense, 재래종 및 저항성 고추 유전자원에서 역병 저항성 계통 선발)

  • Kim, Jeong-Hoon;Yeo, Seung-Ho;Kim, Dong-Woo;Bae, Su-Yeon;Han, Jeong-Hye;Hwang, Hee-Suk;Kim, Byung-Soo
    • Current Research on Agriculture and Life Sciences
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    • v.20
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    • pp.9-18
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    • 2002
  • A total of 31 C. chinense lines selected from 2000 screening were tested again for resistance to P. capsid but resistance was not found in tile lines. A total of 26 selections from Korean land races tested 2001 spring were tested again for resistance to P. capsici, KC180, KC230, KC195 and KC194 showed moderate resistance to P. capsid. However, it was apparent on the basis of horticultural characteristics that KC180 and KC230 had been naturally crossed with AC2258 and CM334, respectively. CM334 and AC2248 seed lots that were increased in different years were taken out and tested for resistance to improve their genetic purity because the resistant genetic resources have been showing some off-types in tile population. Off-types began to be found in 1992 seed lots and tile proportion and degree of tile offs was increasing with time up to 2001. Plants true to the type in 1992 seed lots were selected and their inbred seeds were mass produced in a net cage in the greenhouse. AC2258 included in the experiment together was uniform. In 1995 seed lots of CM334, plants with resistance to P. capsici and low or no number of lateral branching at cotyledonary axil, although they were off from tile original CM334, were found and selection was applied to breed lines fixed in tile characters.

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Production of Xylooligo-Saccharides and Purification of Extracellular Xylanase from Streptomyces chibaensis J-59 (방선균 Streptomyces chibaensis J-59 Xylanase의 정제 및 자일로 올리고당(Xylooligo-Saccharides)의 생산)

  • Joo, Gil-Jae;Rhee, In-Koo
    • Current Research on Agriculture and Life Sciences
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    • v.14
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    • pp.111-122
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    • 1996
  • S. chibaensis J-59 produced an extracellular xylanase in a CSL medium composed of 1.5% com steep liquor, 0.1% $MgSO_4{\cdot}7H_2O$, 0.012% $CoCl_2{\cdot}6H_2O$, and 0.15% glucose containing xylan. but it did not produce in the culture medium containing xylose. The production of enzyme reached to a maximum level (0.83 uints/ml) when bacteria were cultured in 2.5 l jar fermentor for 48hrs at $30^{\circ}C$ and pH 7.0. Furthermore, S. chibaensis J-59 produced an intracellular glucose isomerase in a medium containing xylan and/or xylose. Xylanase was purified 29-fold over the culture supernatants of S. chibaensis J-59 by ammonium sulfate fractionation, chromatography on DEAE-Sephadex A-50, and gel filtration on Sephadex G-200. The purified enzyme is a monomeric enzyme with a native molecular mass of 25 kDa and a subunit molecular mass of 25 kDa. The purified enzyme requires $Mg^{2+}$ for activity, $Ca^{2+}$, $Co^{2+}$ is not an inhibitor but inhibit by $Fe^{3+}$, $Hg^{2+}$, and $Cu^{2+}$, sodium dodecyl sulfate, N-bromosuccinide. Pattern of hydrolysis demonstrated that the xylanase was an endo-splitting enzyme able to break down birchwood xylan at random giving xylobiose, xylotriose and xylotetrose as the main end products.

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Development of Cleaved Amplified Polymorphic Sequence (CAPS) Marker for Selecting Powdery Mildew-Resistance Line in Strawberry (Fragaria×ananassa Duchesne) (딸기 흰가루병 저항성 계통 선발을 위한 분자마커 개발)

  • Je, Hee-Jeong;Ahn, Jae-Wook;Yoon, Hae-Suk;Kim, Min-Keun;Ryu, Jae-San;Hong, Kwang-Pyo;Lee, Sang-Dae;Park, Young-Hoon
    • Horticultural Science & Technology
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    • v.33 no.5
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    • pp.722-729
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    • 2015
  • Powdery mildew (PM) caused by Podosphaera aphanis is a major disease that can result in significant yield losses in strawberry (Fragaria ${\times}$ ananassa Duchesne). For preventing PM, pesticides are usually applied in strawberry. In this study, molecular markers were developed to increase breeding efficiency of PM-resistance cultivars by marker-assisted selection (MAS). An $F_2$ population derived from a cross between PM-resistance 'Seolhyang' and PM-susceptibility 'Akihime' was evaluated for disease resistance to PM and RAPD (random amplification of polymorphic DNA)-BSA (bulked segregant analysis). Among 200 RAPD primers tested, OPE10 primer amplified a 311bp-band present in with 331bp. Sequence alignment performed for searching polymorphisms and six single nucleotide polymorphism (SNP) were found in amplified regions. To develop polymorphic marker for distinguishing between resistant and susceptible, RAPD was converted to cleaved amplified polymorphic sequence (CAPS) marker. Among restriction enzymes associated with six SNPs, Eae I (Y/GGCCR) was successfully digested to 231bp in susceptible. The results suggest that the selected CAPS marker could be used for increasing efficiency of selecting powdery mildew resistant strawberry in breeding system.

A Web-based Simulation Environment based on the Client/Server Architecture for Distance Education: SimDraw (원격교육을 위한 클라이언트/서버구조의 웹 기반 시뮬레이션 환경 : SimDraw)

  • 서현곤;사공봉;김기형
    • Journal of KIISE:Software and Applications
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    • v.30 no.11
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    • pp.1080-1091
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    • 2003
  • Recently, the distance education has been rapidly proliferated with the rapid growth of the Internet and high speed networks. There has been relatively much research with regard to online lecture (teaching and studying) tools for the distance education, compared to the virtual laboratory tools (for self-study and experiments). In this paper, we design and implement a web-based simulation tool, named as SimDraw, for the virtual laboratory in the distance education. To apply the web-based simulation technology into the distance education, some requirements should be met; firstly, the user interface of the simulation should be very easy for students. Secondly, the simulation should be very portable to be run on various computer systems of remote students. Finally, the simulation program on remote computers should be very thin so that students can easily install the program onto their computers. To meet these requirements, SimDraw adopts the client/server architecture; the client program contains only model development and animation functions so that no installation of a client program onto student's system is required, and it can be implemented by a Java applet in Web browsers. The server program supports client programs by offering the functions such as remote compiling, model storing, library management, and user management. For the evaluation of SimDraw, we show the simulation process using the example experimentation of the RIP(Routing Information Protocol) Internet routing protocol.

Design and Implementation of the Management System of Cultivation and Tracking for Agricultural Products using USN (유비쿼터스 센서 네트워크를 이용한 농산물 재배관리 및 이력추적 시스템의 설계 및 구현)

  • Yoo, Nam-Hyun;Song, Gil-Jong;Yoo, Ju-Hyun;Yang, Su-Yeong;Son, Cheol-Su;Koh, Jing-Wang;Kim, Won-Jung
    • Journal of KIISE:Computing Practices and Letters
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    • v.15 no.9
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    • pp.661-674
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    • 2009
  • Recently, there has been much research and many attempts to enhance converged information technology services using new technology such as ubiquitous sensor networks (USN) in medical, environmental, industrial, and logistic areas. There has also been much research and various attempts to apply this new technology to agricultural areas. However, applications to the agricultural areas should be considered differently against the same areas such as medical, environmental, industrial, and logistics. Therefore, this paper suggests that an agricultural cultivating management and traceability system. This system is a unified system that supports the processes sowing seeds through selling agricultural products to consumers. Farmers can be provided with an effective calendar for cultivation and weather information in real time as well as the monitoring of the growth of farm products on the farm in real time using this system. Farmers can also control all equipment installed on the farm directly or remotely and the equipment can be controlled automatically when the measured values such as temperature and humidity deviate from the decent criteria which are set by farmers or this system. Additionally, the reliability for and the better quality of the agricultural products can be improved because farmers can use this unified system to cover all processes from sowing seeds to selling to consumers.

A Basic Study on the Development of Garlic Seeder (경운기 부착형 마늘 파종기 개발을 위한 기초연구)

  • Lim, Hack-Kyoo;Kim, Tae-Han
    • Current Research on Agriculture and Life Sciences
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    • v.22
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    • pp.19-27
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    • 2004
  • Cultivation area of garlics is 9% of all cultivation area of vegetables. The amount of annual demand is increased in 1,000~1,500ton. Also, the amount of demand per person has a tendency to increase as above 10kg. So, garlics has become important crops in agriculture. The purpose of this study is to acquire the basic information to design the garlic seeder attached power tiller. We used Working Model 3D program to design an automatic aligning device which is the most important part of the garlic seeder. The results are as follows; 1. The optimum depth of garlics seeding was shown as 3cm. 2. The garlic seed has to he discharged from the garlic seeder at intervals of 0.75sec in order to seed at intervals of seeds of 15cm. 3. The optimum design factors of the automatic aligning device were shown as cylinder diameter of 4cm, cylinder gap of 1cm, revolution of 36rpm and inclined angle of cylinder of $8.4^{\circ}$.

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Hallym Jikimi: A Remote Monitoring System for Daily Activities of Elders Living Alone (한림 지킴이: 독거노인 일상 활동 원격 모니터링 시스템)

  • Lee, Seon-Woo;Kim, Yong-Joong;Lee, Gi-Sup;Kim, Byung-Jung
    • Journal of KIISE:Computing Practices and Letters
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    • v.15 no.4
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    • pp.244-254
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    • 2009
  • This paper describes a remote system to monitor the circadian behavioral patterns of elders who live alone. The proposed system was designed and implemented to provide more conveniently and reliably the required functionalities of a remote monitoring system for elders based on the development of first phase prototype[2]. The developed system is composed of an in-house sensing system and a server system. The in-house sensing system is a set of wireless sensor nodes which have pyroelectric infrared (PIR) sensor to detect a motion of elder. Each sensing node sends its detection signal to a home gateway via wireless link. The home gateway stores the received signals into a remote database. The server system is composed of a database server and a web server, which provides web-based monitoring system to caregivers (friends, family and social workers) for more cost effective intelligent care service. The improved second phase system can provide 'automatic diagnosis', 'going out detection', and enhanced user interface functionalities. We have evaluated the first and second phase monitoring systems from real field experiments of 3/4 months continuous operation with installation of 9/15 elders' houses, respectively. The experimental results show the promising possibilities to estimate the behavioral patterns and the current status of elder even though the simplicity of sensing capability.

인터넷 질의 처리를 위한 웨이블릿 변환에 기반한 통합 요약정보의 관리

  • Joe, Moon-Jeung;Whang, Kyu-Young;Kim, Sang-Wook;Shim, Kyu-Seok
    • Journal of KIISE:Databases
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    • v.28 no.4
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    • pp.702-714
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    • 2001
  • As Internet technology evolves, there is growing need of Internet queries involving multiple information sources. Efficient processing of such queries necessitates the integrated summary data that compactly represents the data distribution of the entire database scattered over many information sources. This paper presents an efficient method of managing the integrated summary data based on the wavelet transform and addresses Internet query processing using the integrated summary data. The simplest method for creating the integrated summary data would be to summarize the integrated data sidtribution obtained by merging the data distributions in multiple information sources. However, this method suffers from the high cost of transmitting storing and merging a large amount of data distribution. To overcome the drawbacks, we propose a new wavelet transform based method that creates the integrated summary data by merging multiple summary data and effective method for optimizing Internet queries using it A wavelet transformed summary data is converted to satisfy conditions for merging. Moreover i the merging process is very simpe owing to the properties of the wavelet transform. we formally derive the upper bound of the error of the wavelet transformed intergrated summary data. Compared with the histogram-based integrated summary data the wavelet transformedintegrated summary data provesto be 1.6~5.5 time more accurate when used for selectivity estimation in experiments. In processing Internet top-N queries involving 56 information sources using the integrated summary data reduces the processing cost to 1/44 of the cost of not using it.

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The Production of Traditional Alcoholic Beverage in Containing Medicinal Herb (한약재를 첨가한 전통주개발에 관한 연구)

  • Kim, Young-Sook;Park, Young-Sook
    • Food Science and Industry
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    • v.40 no.2
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    • pp.83-89
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    • 2007
  • 저온발효와 고온발효에 의하여 만들어진 민속주를 증류하여 알콜돗를 45%로 같이 맞추어 성분함량을 비교하였다. 발효온도에 따른 환원당의 함량을 나타내고 있으며 저온발효 민속주는 15 mg/100 mL, 고온발효 민속주는 5 mg/100 mL로 나타났다. 즉 저온 발효에서 환원당의 양이 많았으며, 그러나 시중에 판매되고 있는 소주보다는 낮은 경향을 보였다. 향기 성분에 생성과정에 영향을 주는 Fusel alcohol을 볼 때 반적으로는 발효액에 아미노산이 없는 것보다는 많을 수록 많은 양의 fusel alcohol을 생성되기는 하지만, fusel alcohol을 생성하기위한 조건은 효모의 종류, 발효조건, 발효온도, 발효액의 조성 등의 복잡한 인자가 관련된다. 본 연구에 제조한 발효주를 소주의 아미노산과 비교할 때 아미노산 함량이 전반적으로 20-100도 낮은 함량치로 나타났다. 한약재 첨가로 인하여 면역증강, 살세포 반응 억제효과, 기능성 전통주로 좋은 발효주로 인정되지만 한약재특유의 강한 향기를 약화시키는 연구가 금후에 보완되어야 한다고 본다. 발효온도에 따른 아미노산의 함량은 저온발효 조미주에서 cysteine, valine, mrthionine, isoleucine, phenylalanine이 많았으며, 고온발효 조미주에서는 serine, glycine, leucine이 많았다. 한 alanine, tyrisine, lysine은 고온발효주에는 있으나, 저온발효주에서는 나타나지 않았다. 그러나 저온발효주와 고온 발효주 모두 시중에 판매되고 있는 소주보다는 아주 낮은 경향을 보였다. 저온발효와 고온발효에 의하여 만들어진 민속주를 증류하여 알콜 도수를 45%로 같이 맞추어 성분함량을 비교하였다. 발효온도에 따른 환원당의 함량을 나타내고 있으며 저온발효 민속주는 15 mg/100 mL, 고온발효 민속주는 5 mg/100 mL로 나타났다. 즉 저온 발효에서 환원당의 양이 많았으며, 그러나 시중에 판매되고 있는 소주보다는 낮은 경향을 보였다. 아미노산 실험에서는 소주와 45% 고온 발효주를 비교 할 때 serine, glycine, cysteine, methionine, phenylalanine 등은 100배, alanine, valine, isoleucine 80배, ammonia 20배 낮은 수치를 나타냈다. OD 측정의 실험결과 또한 control과 비교시 40% 발효주에서는 10배, 45% 고은 발효주에서는 100배 낮은 측정치가 나타났으므로 한약재의 색소성분을 휘발시키는 기술이 요구된다고 본다. 항균성실험에서 항균성 측정은 06 cm paper disk agar diffusion법을 이용하였으며, 43%의 발효주와, 45% 고온 발효주가 항균력이 가장 강력한 0.95 cm의 영향을 나타냈다. 사용한 사용한 Gram 양성, Gram 음성 균주는 Escherichia coli KCCM 11591를 제외하고는 0.8 - 0.95 cm로 항균력이 강했으며, Gram negitive의 Pseudomonas aeruginosa KCTC 1750 에서는 43% 발효주에는 0.95 cm, 45% 고은 발효주에는 0.95 cm의 항균성을 나타냈으며 관능평가에서도 가장 높게 났다. 관능평가에서는 45% 고온 발효주가 가장 높게 나타났으며, 항산화성 실험에 나타난 저온 45%의 갈색도의 측정과는 항산화성에서는 좀 다른 결과를 나타낸다. 그러나 항균성이 가장 높게 나타난 43-45%와 관능평가에서 가장 높게 나타난 45% 고온 발효주를 볼 때 본 연구에서는 고온 발효주 45%가 가장 우수한 전통주로 조사되었다. 발효주를 소주의 아미노산과 비교할 때 아미노산 함량이 전반적으로 100배 정도 낮은 함량치로 나타났으므로, 한약재 첨가로 인하여 면역증강, 살세포반응 억제효과, 기능성전통주로 좋은 발효주로 인정되지만 한약재특유의 강한 향기를 약화시켜서 부드럽고 은은한 전통발효주의 연구가 금후에 보완되어야 한다고 본다.