• Journal of KIISE:Computer Systems and Theory
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• v.33 no.3
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• pp.157-165
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• 2006

The change of external/internal factors of the cell rquires specific biological functions to maintain life. Such functions encourage particular genes to jnteract/regulate each other in multiple ways. Accordingly, we applied a linear decomposition model IFSA, which derives hidden variables, called the 'expression mode' that corresponds to the functions. To interpret gene interaction/regulation, we used a cross-correlation method given an expression mode. Linear decomposition models such as principal component analysis (PCA) and independent component analysis (ICA) were shown to be useful in analyzing high dimensional DNA microarray data, compared to clustering methods. These methods assume that gene expression is controlled by a linear combination of uncorrelated/indepdendent latent variables. However these methods have some difficulty in grouping similar patterns which are slightly time-delayed or asymmetric since only exactly matched Patterns are considered. In order to overcome this, we employ the (IFSA) method of [1] to locate phase- and shut-invariant features. Membership scoring functions play an important role to classify genes since linear decomposition models basically aim at data reduction not but at grouping data. We address a new function essential to the IFSA method. In this paper we stress that IFSA is useful in grouping functionally-related genes in the presence of time-shift and expression phase variance. Ultimately, we propose a new approach to investigate the multiple interaction information of genes.

• Journal of KIISE:Computer Systems and Theory
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• v.34 no.5_6
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• pp.257-266
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• 2007

In On-Chip bus, the arbitration scheme is one of the critical factors that decide the overall system performance. The arbitration scheme used in traditional shared bus is the master-side arbitration based on the request and grant signals between multiple masters and single arbiter. In the case of the master-side arbitration, only one master and one slave can transfer the data at a time. Therefore the throughput of total bus system and the utilization of resources are decreased in the master-side arbitration. However in the slave-side arbitration, there is an arbiter at each slave port and the master just starts a transaction and waits for the slave response to proceed to the next transfer. Thus, the unit of arbitration can be a transaction or a transfer. Besides the throughput of total bus system and the utilization of resources are increased since the multiple masters can simultaneously perform transfers with independent slaves. In this paper, we implement and analyze the arbitration schemes for the Multi-Layer AHB BusMatrix based on the slave-side arbitration. We implement the slave-side arbitration schemes based on fixed priority, round robin and dynamic priority and accomplish the performance simulation to compare and analyze the performance of each arbitration scheme according to the characteristics of the master and slave. With the performance simulation, we observed that when there are few masters on critical path in a bus system, the arbitration scheme based on dynamic priority shows the maximum performance and in other cases, the arbitration scheme based on round robin shows the highest performance. In addition, the arbitration scheme with transaction based multiplexing shows higher performance than the same arbitration scheme with single transfer based switching in an application with frequent accesses to the long latency devices or memories such as SDRAM. The improvements of the arbitration scheme with transaction based multiplexing are 26%, 42% and 51%, respectively when the latency times of SDRAM are 1, 2 and 3 clock cycles.

• Horticultural Science & Technology
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• v.28 no.6
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• pp.1025-1038
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• 2010

Carrot ($Daucus$ $carota$ L. var. $sativa$) is one of the most widely used crops in the world. Moreover it is an important crop because of its high content of ${\beta}$-carotene, well-known as the precursor of vitamin A carotenoid. However, seed-hair which is generated in epidermal cell of seeds inhibits absorption and germination. For that reason, carrot seeds are commercialized after mechanical hair removal process. To overcome such cumbersome weaknesses, new breeding program for developing hairless-seed carrot cultivar has been needed. Therefore, in this study, cDNA libraries from seeds of short-hair seed phenotype CT-ATR615 OP 666-13line and hairy seed CT-ATR615 OP-CK1-9 line were constructed and expression patterns related to generation of seed-hair were analyzed by comparison of EST sequences. Differential EST sequence results between two lines were classified into FunCat functional categories based on the results of BlastX search. Higher expression quantities belonging to metabolic category were shown on short-hair seed line than hairy-seed one. Differential expression quantities between those two lines in the protein folding and stabilization, subcellular localization categories were supposed to contribute variously on the generation of seed-hair. We confirmed 50 and 59 SSR sites, and 2 SNP sites by analyzing EST sequences in two lines; thereafter, we designed SNP and SSR primer sets from these EST sequence information as a molecular marker. These markers are thought to be used in research of molecular markers for classification of carrot family and related to various traits, as well as seed-hair characteristic.

• The Korean Journal of Pesticide Science
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• v.18 no.4
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• pp.247-257
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• 2014

Assessment for operator's dermal and inhalation exposure to acetamiprid during cultivation of water melon in greenhouse was carried out. For dermal exposure measurement, whole body dosimetry (WBD) was performed as the first trial in Korea. WBD consists of cotton/polyester outer clothes and cotton inner clothes. Hand exposure was measured by washing of nitrile gloves and hands while head exposure was monitored by face/neck wipe technique. Inhalation exposure was monitored with personal air sampling pumps and IOM sampler (glass fiber filter). Analytical limit of quantitation was 2.5 ng/mL. Good reproducibility (C.V < 8.7%), linearity ($R^2$ > 0.99) and recovery (70~119%) were obtained. Field recovery of acetamiprid was 77~95%. During mixing/loading, hand exposure of acetamiprid was about 10 times ($229.7{\mu}g$) more than that of application case ($20.9{\mu}g$). During application, total dermal exposure was $1207.4{\mu}g$. Exposure of lower legs was $1132.1{\mu}g$, which is 93.8% of the total dermal exposure. Inhalation exposure during mixing/loading and application was not detected. Margin of safety (MOS) was calculated for risk assessment using male Korean average body weight (70 kg) and acceptable operator exposure level ($124{\mu}g/kg/day$) to give 140, suggesting that health risk of operator during treatment of acetamiprid for water melon in greenhouse could be safe.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.3
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• pp.320-330
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• 2018

This study analyzed the curriculum and clinical practicum of the department of clinical laboratory science in Korea. The 2017 educational curricula of all 3-year and 4-year universities were analyzed. Clinical practice was conducted by a questionnaire. As a result of curriculum analysis, the 3-year curriculum was able to grasp the curriculum that focused on the national examinations of medical technologists, and the 4-year curriculum was open to a variety of subjects reflecting the changes in the future, but only at a few universities. In addition, the autonomous enrollment application made it possible to take a national examination without enrolling in courses that students find difficult in major courses. In the case of clinical practice, it was difficult to standardize in various practical institutes, practice periods, and practice credits. Therefore, it will be necessary to standardize the composition of the credits according to the duration of clinical practice and the duration of education. Moreover, is necessary to revise the curriculum in consideration of medical technologist job analysis and clinical field, and it will be necessary to standardize the curriculum and clinical practice model through the Medical Technologist Evaluation Center.

• Horticultural Science & Technology
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• v.19 no.3
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• pp.378-383
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• 2001

Embryo culture, ovule culture and ovary slice culture were tested to find optimum method for overcoming post fertilization barrier in interspecific crosses between L. longiflorum 'Gelria' and L. cernuum. Although reciprocal crosses between the species were carried out by cut-style pollination method, fruits developed only in crosses of L. longiflorum${\times}$L. cernuum. On the 40 days after pollination, ovaries were sliced into 2-4mm thickness and cultured on a hormone-free Murashige-Skoog (MS) medium, supplemented with 2%, 4%, 6%, 8% and 10% sucrose. For the L. longiflorum Gelria'${\times}$L. cernuum cross, ovule development was found to be best at 6% sucrose and a lot of hybrid plant lets established directly from the ovary slice culture and subsequent ovule culture. High concentration of sucrose above 8% made ovules abort or vitrificate from 40 days after culture. In contrast, ovules from the L. cernuum${\times}$L. longiflorum 'Gelria' cross swelled well in ovary slice culture, however, they did not germinated in subsequent ovule culture. On the 60 days after pollination, ovules thicker than 0.6mm was interpreted as one containing embryo. The embryo size ranged from 1.2 mm to 1.7 mm, and in vitro development of the excised embryos was found to be best with the MS medium (pH 5.8), supplemented with $0.1-1 mg{\cdot}L^{-1}$ NAA and 6% sucrose. Thick ovules excised 60 days after pollination germinated about 60% as normal seeds in MS medium supplemented with 6% sucrose and free hormone. The ovule culture 60 days after pollination was concluded to be most recommendable to produce interspecific hybrids in large scale crosses between L. longiflorum 'Gelria' and L. cernuum by the reason of easy procedure.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.202-209
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• 2014

This study was conducted to investigate the effects of low temperature shipping condition and precooling treatments (forced air and room cooling) on everbearing strawberry through export simulation of 'Goha' and 'Flamenco' cultivars. After harvest, it took two days to prepare export procedure such as precooling, sorting, storage, transportation, quarantine and handling, and then everbearing strawberry was carried out shipping at room ($20-25^{\circ}C$) or low ($8^{\circ}C$) temperature conditions. In the case of shipping at room temperature, weight losses of both cultivars were increased up to 10% after 2 days where 'Goha' being 2% higher than that of ' Flamenco'. In 'Flamenco' cultivar, shipping temperature and precooling treatment were not effective in firmness and soluble solids contents during transporting periods. However, in 'Goha' cultivar, room cooling treatment and low shipping temperature were effective in maintaining firmness until 4 days after shipping. Especially titratable acidity was affected by shipping temperature ($P{\leq}0.001$) and precooling treatments ($P{\leq}0.05$) in 'Goha' cultivar. Also shipping temperature under $8^{\circ}C$ delayed coloring and decay incidence of both strawberry cultivars, and precooling treatments of both forced air and room cooling reduced frequency of decay. The shelf life of everbearing strawberry at low shipping temperature was extended more than 4 days compared with shipping at room temperature. Precooling treatment including forced air or room cooling will be useful for the two cultivars when they are transported at low temperature. In 'Flamenco' cultivar, the effect of forced air and room cooling was similar, whereas in 'Goha' room cooling was more effective.

• Journal of Internet Computing and Services
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• v.5 no.5
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• pp.1-15
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• 2004

In this paper, we explain the design and implementation of GWB(Gene WorkBench), which is a web-based, integrated system for efficiently managing and analyzing genomic sequences, Most existing software systems handling genomic sequences rarely provide both managing facilities and analyzing facilities. The analysis programs also tend to be unit programs that include just single or some part of the required functions. Moreover, these programs are widely distributed over Internet and require different execution environments. As lots of manual and conversion works are required for using these programs together, many life science researchers suffer great inconveniences. in order to overcome the problems of existing systems and provide a more convenient one for helping genomic researches in effective ways, this paper integrates both managing facilities and analyzing facilities into a single system called GWB. Most important issues regarding the design of GWB are how to integrate many different analysis programs into a single software system, and how to provide data or databases of different formats required to run these programs. In order to address these issues, GWB integrates different analysis programs byusing common input/output interfaces called wrappers, suggests a common format of genomic sequence data, organizes local databases consisting of a relational database and an indexed sequential file, and provides facilities for converting data among several well-known different formats and exporting local databases into XML files.

• Analytical Science and Technology
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• v.11 no.5
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• pp.347-352
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• 1998

A study was carried out on the elelctrochemical characteristics of chemically modified electrodes (CMEs) by cyclic voltammetry. Fabrication of CMEs was made by coating with mixed valence (mv) inorganic-metal polymeric films on the glassy carbon electrode surface by potential cycling. Anodic oxidation behavior of methanol and L-ascorbic acid was studied by using CMEs working electrode. Deposition of films such as mv ruthenium oxo/ruthenium cyanide film (mv Ru-O/CN-Ru), mv ruthenium oxo/ferrocyanide film (mv Ru-O/$Fe(CN)_6$), and mv ruthenium oxo/ruthenium cyanide/Rhodium film (mv Ru-O/CN-Ru/Rh) was obtained to coat by scan rate of 50 mV/sec within the specified potential range (-0.5V ~ +1.2V). Film thickness was controlled by the repeat of the potential cycling. Anodic oxidation behavior of methanol was as follow. Calibration graph by using mv Ru-O/CN-Ru film showed linearly from 10 mM to 80 mM MeOH with slope factor of $-7.552{\mu}A/cm^2$. Although slope factor by using mv Ru-O/$Fe(CN)_6$ film was $-5.13{\mu}A/cm^2$, yet linear range of calibration graph could be extended from 10 mM to 100 mM MeOH. Anodic oxidation behavior of L-ascorbic acid was studied by mv Ru-O/CN-Ru film on the glassy carbon electrode and the glassy carbon electrode with Rh film, Glassy carbon electrode modified with Ru polymeric film was showed better sensitivity than the Rh-glassy carbon modified electrode (mv Ru-O/CN-Ru/Rh). Calibration graph was linear from 0.1 mM to 5 mM L-ascorbic acid by using glassy carbon electrode modified with Ru polymeric film. Solpe factor and relative coefficient are $-84.78{\mu}A/mM$ and 0.998, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.12 no.3
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• pp.125-130
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• 1979

In this paper, the noise pressure propagated in the air on account of the engine revolution of a stern trawler, Sae-Ba-Da(G. T. 2275.71) was measured at the check points No.1 through No.43 when the vessel was cruising, towing nets, and drifting. The experiment was carried out in the period from August 23 to October 22, 1978 at the locations of lat. $33^{\circ}$ 47'N, long. $127^{\circ}$ 34'E; lat. $34^{\circ}$ 24'N, long. $128^{\circ}$ 23'E; and lat. $6^{\circ}$ 01'N, long. $108^{\circ}$ 04'E. In case of cruising, noise on the weather deck came from funnel noise. The highest noise pressure was 92dB at observation point No.9 where tile noise pressure from main engine was 105dB when the engine was operated at 730rpm and $12^{\circ}$ sorely propeller pitch. The noise measured was reduced to 90dB at observation point No.9 when the screw propeller pitch was changed to $8^{\circ}$ that resulted in reduction of engine to 103dB. In case of towing net, the main engine revolution and screw propeller pitch was fixed at 730rpm and $8^{\circ}$ respectively. But the engine noise pressure was increased up to 106dB due to the towing resistance by 14 tons of the nets, and the noise pressure was 90dB at No.9 point. A hight noise was also generated from screw because of the towing reoistance and could be measurable even in the wall of the insulated freezing room. When the vessel was drifting: the noise pressure from the generator operated, at 720rpm was 100dB. This caused 87dB noise pressure at No.9 point. The noise pressure in the boarding or residence sections was 45 to 60dB in each case of cruisinrg towing net or drifting but it was so high as 82dB on the open deck that voice could hardly be heap.