• Journal of Life Science
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• v.32 no.1
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• pp.29-35
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• 2022

This study was conducted to analyze the genetic polymorphisms of dopamine receptor D4 (DRD4) in horse breeds and its association with substrate characteristics in Jeju crossbreds (Jeju Horse × Thoroughbred). Polymorphisms in DRD4 are candidate genes associated with temperament in various mammals, including humans. Single nucleotide polymorphism (SNP) G292A in the exon 3 region of the horse DRD4 has a reported association with curiosity and vigilance in thoroughbreds. Sanger sequencing was used to identify polymorphisms of the mutations in DRD4 in three horse breeds. The SNP frequency in Jeju horses was significantly different from the frequency in other breeds. Character evaluation, conducted in the Jeju crossbreds and scored using a temperament test and contact test, revealed a high correlation between each test. Comparison of the polymorphism in the DRD4 of horses and the results of the character evaluation revealed lower scores for all temperaments in horses carrying allele A. Comparison of the SNP of G292A and blood dopamine levels in Jeju crossbreds showed 2.87 times higher levels for the GA type than for the GG type. This study identified an association between DRD4 polymorphism and various test methods for evaluating horse temperament and levels of neurotransmitters. Further research could validate the use of this gene as a genetic marker for character evaluation.

• Therapeutic Science for Rehabilitation
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• v.11 no.2
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• pp.53-62
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• 2022

Objective : This study aimed to present basic data that could help in selecting or using evaluation tools in clinical settings. Methods : This study included 51 patients with stroke. The Cognitive Impairment Screening Test (CIST), Korean-Mini Mental State Examination, 2nd Edition (K-MMSE~2), and Clinical Dementia Rating (CDR) were used as evaluation tools. The correlation between evaluation tool scores was analyzed using Spearman's rank correlation coefficient, and the comparison of total scores between the CIST and K-MMSE~2 according to global CDR scores was analyzed using the Wilcoxon signed-rank test. Results : The correlation between the total CIST and K-MMSE~2 scores and global CDR scores was statistically significant (p<.01). The correlation between the sub-scores of the CIST and K-MMSE~2 showed a statistically significant correlation for all sub-scores (p<.01). The comparison of total scores between the CIST and K-MMSE~2 according to global CDR scores showed no statistically significant differences in all global CDR scores. Conclusion : This study showed that there was a correlation between CIST, K-MMSE~2, and CDR in patients with stroke. In the future, we hope that the results of this study will help to select or use cognitive function evaluation tools in clinical settings.

• Journal of Ginseng Culture
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• v.4
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• pp.128-141
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• 2022

Baengnyeongdo Island, which belongs to Ongjin-gun, Incheon, is an island in the northernmost part of the West Sea in South Korea. Baengnyeong Island is the 15th largest island in Korea and covers an area of 51 km². The Korea Ginseng Corporation (KGC) investigated the possibility of growing ginseng on Baengnyeong Island in 1996. In 1997, thanks to the support of cultivation costs from Ongjin-gun, the first ginseng seedbed was built on Baengnyeong Island. In 1999, the seedlings were transplanted to a permanent field under a contract with KGC. In 2003, the first six-year-old ginseng harvest was performed, and KGC purchased all production according to the contract. Since then, KGC has signed on to grow ginseng until 2012 and purchased six-year-old ginseng until the fall of 2016. Since 2014, the GimpoPaju Ginseng Agricultural Cooperative Association has signed a ginseng production contract. According to a survey of nine 6-year-old ginseng fields (total 5,961 units) on Baengnyeong Island, the top five with good growth had a survival rate of 42.6 to 68%, and the bottom four with poor growth had an extremely low survival rate of 11.1 to 21.3%. The four fields with low survival rates were where hot peppers were planted before ginseng cultivation. It is believed that the excess nitrogen remaining in the soil due to the treatment of compost or manure during pepper cultivation causes ginseng roots to rot. The average incidence of Alternaria blight was 8.6%. Six six-year-old ginseng gardens were low at 1.1 to 4.7%, while the other three were high at 16.7 to 20.9%. It is assumed that the reason for the low survival rate and high incidence of Alternaria blight is a rain-leaking shield. Farmers used rain-leaking shields because the precipitation on Baengnyeong Island was smaller than on land. One field showed 3% of leaves with yellowish brown spots, a symptom of physiological disturbance of the leaf, which is presumed to be due to the excessive presence of iron in the soil. To increase the production of ginseng on Baengnyeong Island, it is necessary to develop a suitable ginseng cultivation method for the island, such as strengthening the field management based on the results of a scientific study of soil, using rain-resistant shading, and installing drip irrigation facilities. I hope that ginseng will become a new driving force for the development of Baengnyeong Island, allowing ginseng products and food to thrive in the beautiful natural environment of the island.

• Journal of Korea Port Economic Association
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• v.38 no.3
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• pp.105-131
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• 2022

Even after the improvement in 2019, UNCTAD's Liner Shipping Connectivity Index (LSCI), which evaluates the performance of the global container port market, has limited use. In particular, since the liner shipping connectivity index evaluates the performance based only on the distance of the relationship, the performance index combining the port attractiveness of calling would be more efficient. This study used the modified Huff model, the hub-authority algorithm and the eigenvector centrality of social network analysis, and correlation analysis for 2007, 2017, and 2019 data of Ocean-Commerce, Japan. The findings are as follows: Firstly, the port attractiveness of calling and the overall performance of the port did not always match. However, according to the analysis of the attractiveness of a port calling, Busan remained within the top 10. Still, the attractiveness among other Korean ports improved slowly from the low level during the study period. Secondly, Global container ports are generally specialized for long-term specialized inbound and outbound ports by the route and grow while maintaining professionalism throughout the entire period. The Korean ports continue to change roles from analysis period to period. Lastly, the volume of cargo by period and the extended port connectivity index (EPCI) presented in this study showed a correlation from 0.77 to 0.85. Even though the Atlantic data is excluded from the analysis and the ship's operable capacity is used instead of the port throughput volume, it shows a high correlation. The study result would help evaluate and analyze global ports. According to the study, Korean ports need a long-term strategy to improve performance while maintaining professionalism. In order to maintain and develop the port's desirable role, it is necessary to utilize cooperation and partnerships with the complimentary port and attract shipping companies' services calling to the complementary port. Although this study carried out a complex analysis using a lot of data and methodologies for an extended period, it is necessary to conduct a study covering ports around the world, a long-term panel analysis, and a scientific parameter estimation study of the attractiveness analysis.

• Journal of Conservation Science
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• v.37 no.6
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• pp.689-700
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• 2021

This study aims to optimize the measurement conditions of handmade Hanji paper to reduce the time required for measurement while meeting folding endurance standards, for various basis weights. Thirteen types were selected, including 12 types of Hanji with grammages of 17 g/m², 30 g/m², and 45 g/m², and one type of 75 g/m² copy paper, and 24 sheets were prepared in the laid and chain directions. Subsequently, folding endurance experiments at different load (14.72 N, 9.81 N, 4.91 N) and specimen width conditions (15 mm, 10 mm, 5 mm) were performed, using a 4-MIT folding endurance tester. The results showed that, for specimen widths of 15 mm and 10 mm under a load of 4.91 N, the folding endurance falls within the reference range (10~10000 times). In particular, reduced folding endurance range and measurement time were observed at a width of 10 mm under a load of 4.91 N. Moreover, the endurance trend lines based on the average values of load and specimen width ranges enabled the prediction of the folding endurance within those ranges, according to the decreasing slope. Furthermore, for a specimen width of 15 mm under a load of 4.91 N, the folding endurance in the comparison group (35 to 17723 times) significantly exceeds the upper limit of the reference range (10~10000 times). Therefore, the measurement conditions of 4.91 N with a specimen width of 10 mm are proposed.

• Journal of the Korean Society of Radiology
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• v.16 no.1
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• pp.35-43
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• 2022

In this study, a survey was conducted and analyzed to find out the satisfaction of online classes among students enrolled in the radiology department of a university located in Busan city. As a result, in terms of satisfaction with online classes, male scores were higher, but there was no statistically significant difference. In the interdisciplinary system, the satisfaction score of the students enrolled in Bachelor's degree was high, and there was a statistically significant difference except for the satisfaction of learning participation (p<0.001, p<0.05). For class satisfaction by grade level, Senior had higher scores, and there were statistically significant differences except for learning participation satisfaction (p<0.001, p<0.01, p<0.05). In the satisfaction survey according to the number of lectures, the scores of the students who took 4-7 lectures were found to be high except for the satisfaction of learning participation, and there was a statistically significant difference (p<0.01, p<0.05). In the method of communication with the instructor, students who used e-mail showed high scores, and there was a statistically significant difference in lecture satisfaction (p<0.05). In the correlation analysis between sub-variables for online classes, statistically significant correlations were established in all areas. Most of the students preferred class methods such as recorded classes and classes using external content such as YouTube, and when asked about the merits of online classes, many students answered that the advantages of online classes were repetitive classes and no restrictions on time and place. When asked about the shortcomings of online classes, many students answered that it was a lack of concentration and lack of communication with the instructor. This study was conducted to provide basic data to improve the satisfaction of online classes that will increase in the future. Therefore, based on the results of this study, it is expected that more quality online classes will be produced so that students' satisfaction with online classes can be improved.

• Journal of Dental Rehabilitation and Applied Science
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• v.38 no.3
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• pp.150-161
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• 2022

Purpose: To determine the effects of the microgroove-fibronectin complex surface on the expression of various genes related to cellular activity in human gingival fibroblasts. Materials and Methods: Smooth titanium specimens (NE0), acid-treated titanium specimens (E0), microgroove and acid-treated titanium specimens (E60/10), fibronectin-fixed smooth titanium specimens (NE0FN), acid-treated and fibronectin-immobilized titanium specimens (E0FN), and microgroove and acid-treated titanium specimens immobilized with fibronectin (E60/10FN) were prepared. Real-time polymerase chain reaction experiments were conducted on 44 genes related to cell behavior of human gingival fibroblasts. Results: Adhesion and proliferation of human gingival fibroblast on microgroove-fibronectin complex titanium were activated through four types of signaling pathway. Integrin α5, Integrin β1, Integrin β3, Talin-2, which belong to the focal adhesion pathway, AKT1, AKT2, NF-κB, which belong to the PI3K-AKT signaling pathway, MEK2, ERK1, ERK2, which belong to the MAPK signaling pathway, and Cyclin D1, CDK4, CDK6 genes belonging to the cell cycle signaling pathway were upregulated on the microgroove-fibronectin complex titanium surface (E60/10FN). Conclusion: The microgroove-fibronectin complex titanium surface can up-regulate various genes involved in cell behavior.

• Journal of Life Science
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• v.32 no.12
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• pp.947-955
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• 2022

Silkworms, which have recently shown promise as functional health foods, show functional differences between varieties; therefore, the need for variety identification is emerging. In this study, we analyzed the whole silkworm genome to identify 10 unique silkworm varieties (Baekhwang, Baekok, Daebaek, Daebak, Daehwang, Goldensilk, Hansaeng, Joohwang, Kumkang, and Kumok) using single nucleotide polymorphisms (SNP) present in the genome as biomarkers. In addition, nine SNPs were selected to discriminate between varieties by selecting SNPs specific to each variety. We subsequently created a decision tree capable of cross-verifying each variety and classifying the varieties through sequential analysis. Restriction fragment length polymorphism (RFLP) was used for SNP867 and SNP9183 to differentiate between the varieties of Daehwang and Goldensilk and between Kumkang and Daebak, respectively. A tetra-primer amplification refractory (T-ARMS) mutation was used to analyze the remaining SNPs. As a result, we could isolate the same group or select an individual variety using the nine unique SNPs from SNP780 to SNP9183. Furthermore, nucleotide sequence analysis for the region confirmed that the alleles were identical. In conclusion, our results show that combining SNP analysis of the whole silkworm genome with the decision tree is of high value as a discriminative marker for classifying silkworm varieties.

• Ecology and Resilient Infrastructure
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• v.9 no.1
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• pp.68-76
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• 2022

In this study, the Habitat Suitability Index (HSI) was calculated in the Miho stream of the Geum river system, and the environmental ecological flow by point was evaluated. Two points (St.3 and St.8) representing the up and downstream of Miho Stream were selected, in order to calculate the Habitat Suitability Index, the depth and velocity at point where each species is appeared were investigated. The Habitat Suitability Index (HSI) was calculated by the Washington Department of Fish and Wildlife (WDFW) method using the number collected by water depth and velocity section and the results of the flow rate survey. Two target species were selected in this study; dominant species and swimming species sensitive to flow. In the case of a single species of Zacco platypus, the water depth was 0.1 - 0.5 m and the velocity was 0.2 - 0.5 m/s. For species of swimming fish, the water depth was 0.2 - 0.5 m and the velocity was 0.2 - 0.5 m/s. The discharge-Weighted Useable Area (WUA) relationship curve and habitat suitability distribution were simulated at the Miho Stream points St.3 and St.8. At the upstream St.3 of Miho Stream, the optimal discharge was simulated as 4.0 m³/s for swimming fishes and 2.7 m³/s for Zacco platypus. At the downstream point of St.8, species of swimming fish were simulated as 8.8 m³/s and Zacco platypus was simulated as 7.6 m³/s. In both points, the optimal discharge of swimming fish was over estimated. This is a result that the Habitat Suitability Index for swimming fish requires a faster flow rate than the habitat conditions of the Zacco platypus. In the calculation of the minimum discharge, the discharge of Zacco platypus is smaller and is evaluated to provide more Weighted Useable Area. In the case of swimming fishes, narrow range of depth and velocity increases the required discharge and relatively decreases the Weighted Useable Area. Therefore, when calculating the Habitat Suitability Index for swimming fishes, it is more advantageous to calculate the index including the habitat of all fish species than to narrow the range.

• Maritime Security
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• v.5 no.1
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• pp.113-136
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• 2022

China has been pushing for a systematic strategy for territorialization over a long period of time to invade Korea's West Sea (Yellow Sea) in order to create China's territorial water. China's strategy for territorializing the West Sea is an activity in which China curbs the use of South Korea and enforces the illegal use of China in order to dominate the West Sea exclusively. China aided Chinese fishing boats that engaged in illegal fishing in Korea's jurisdiction as a means to territorialize the West Sea, and is opposed to combined exercise and training of Korea and the United States Naval Forces in the West Sea, while intentionally entering KADIZ(Korea Air Defense Identification Zone). In addition, Beijing used 'scientific exploration and research' measures as a pretext for its strategies in order to encroach on Korea's West Sea. China is carrying out such work to announce to the world that China is a systematic and organized country while consistently attempting to dominate the West Sea. China's activities in the West Sea seriously infringe South Korea's sovereignty. In order to respond to China's strategies of territorialization in the West Sea stated above, I analyzed the rejection effect of the ROK-US combined military training in the West Sea and presented a 'proportional response strategy centered on the ROK-US combined forces'. Korea should be able to respond proportionally to China's activities in the seas around the Korean peninsula, and Korea should be able to neutralize China's attempt to a Fait Accompli. In addition, just as China installs buoys in the Korea-China Provisional Measures Zone, Korea should be able to install and actively utilize some devices in the West Sea and for the use of free and open West Sea. Korea should not just wait for the tragic future to come without preparing for China's gradual and long-term strategy, and Seoul needs to respond to China's maritime policy in the West Sea with a more active attitude than it is now. China has historically taken a bold and aggressive response to neighboring countries that are consistent with a passive attitude, on the other hand, Beijing has taken a cautious approach to neighboring countries that respond with an active attitude. It should not be forgotten that Korea's passive response to the Chinese strategy in the name of a 'realistic approach' such as Korea's economic dependence on China for economy will result in China's success for territorialization of the West Sea.