• Title/Summary/Keyword: 과립형

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한국산 플라나리아 Phagocata vivida 인두에서 형성되는 점액과립

  • 장남섭
    • The Korean Journal of Zoology
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    • v.36 no.4
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    • pp.496-504
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    • 1993
  • 한국산 플라나리아 Phagocata vivida의 인두에서 다음과 같은 점액과립들을 관찰하였는데 그 결과는 다음과 같다. 인두외강의 인두측 상피조직과 내측부의 유조직에서 조직화학적 및 미세구조적 특징에 따라 5종류의 점액과립(G1형, G2형, G3형, G4형 및 G5형)이 관찰되었다. methylene blue-baslc fuchsin 이중염색을 시행한 결과 G1형, G3형 및 G4형 과립은 methylene blue에만 양성반응을 보인데 비해 G2형 및 G5형 과립은 두 염색액에 모두 양성반응을 보였다. G1형과 G3형 과립은 그 크기가 0.5mm정도이고. 전자밀도가 매우 높게 나타났으며 특히 G1형 과립에서는 흰 반점과 줄무의가 관찰되었다 G2형 과립은 그 크기가 0.750m정도이고, 전자밀도는 G3형 과립에 비해 약간 낮았다. G4형 과립은 그 크기가 0.3mm정도로 매우 작고, 전자밀도는 낮아서 밝게 보였다. G5형 과립은 그 크기가 0.4$\times$0.3mm정도이고. 전자밀도가 낮게 나타났으며 배상세포 형태를 하고 있었다.

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Ultrastructural and Histochemical Study on the Epithelia of Digestive Tract of a Korean Slug, Incilaria fruhstorferi (한국산 산민달팽이 ( Incilaria fruhstorferi ) 소화관 상피조직의 미세구조 및 조직화학적 연구)

  • 이정찬;장남섭;한종민
    • The Korean Journal of Malacology
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    • v.13 no.2
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    • pp.143-160
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    • 1997
  • 한국산 산민달팽이 Incilariafruhstorferi의 소화관을 부위별로 관찰하고, 소화관 상피조직을 구성하는 세포의 종류와 분포수 그리고 분비되는 과립 등을 미세구조적, 조직화학적으로 관찰하고 그 결과를 보고하고자 한다. 산민달팽이의 소화관은 식도, 위, 장, 직장 등으로 구성되어있으며, 식도는 다시 전식도, 소낭, 후식도로 나누어지고, 장은 전장과 중장, 후장 등으로 구분되었다. 소화관을 부위별롸 관찰한 결과 섬모원주상피세포 2종, 녹색과립세포 3종, 청색과립세포, 점액세포, 투명세포, 망상형세포 그리고 괴사형세포 등 모두 10종류가 확인되었다. 섬모원주세포는 A형과 B형 등 두 종으로 나뉘며, A형은 세포의 상단 자유면에 섬모와 미세융모가 밀생된데 비해, B형은 전자밀도가 높아서 검게 보이고, 장과 직장에서만 관찰되는 특징을 보였다. 섬모의 구조는 9 x 2 + 2 axoneme이었다. 녹색과립세포는 미세구조에 의해 A형, B형 그리고 C형등 3종으로 나누어지며, 소낭과 후식도, 위, 직장에서 주로 관찰되었다. A형과 B형은 Sudan black에 양성인 지방과립(1.36 x 1.6$\mu\textrm{m}$)만을 포함하고 있는데비해 C형은 글리코겐 과립도 포함하고 있었다. 청색과립세포는 10종류의 세포 중 가장 키가 크고 (35$\mu\textrm{m}$), Millon반응에 양성을 보이는 둥근과립(직경, 1.3$\mu\textrm{m}$)을 포함하고 있는데 과립들은 단백질성으로 확인되었다. 이들은 중장에서만 관찰되었다. 장과 직장에서 주로 많이 관찰되는 점액세포는 세포질 속에 전자밀도가 낮은 투명한 과립과 전자밀도가 높아서 검게 보이는 둥근과립(크기, 1.33 x 0.89$\mu\textrm{m}$)들을 포함하고 있었는데, 이들은 미성숙 시기에는 투명과립(직경, 2.66$\mu\textrm{m}$)으로 관찰되었다. PAS-alcian blue(pH 2.5)반응에서 투명과립은 alcianophillia로 나타난 반면, 검은 과립은 PAS에 양성반을을 보이며, 각각 산성점액과 중성점액과립으로 확인되었다. 투명세포는 광학현미경 관찰에서 A형과 B형으로 구분되었으나, A형은 전자현미경 관찰에서 신경내분비세포로 확인된 반며, B형은 지방과립을 소지한 지방저장세포로 각각 확인되었다. 신경내분비세포가 소지한 과립의 크기는 0.16$\mu\textrm{m}$ 정도였다. 망상형세포는 주로 위에서 관찰되는 형태가 불규칙한 작은 세포로서 세포질에 비해 큰 핵을 소지하고 있었다. 소량의 세포질 돌기에는 사립체와 과립성소포체를 포함하고 있다. 괴사형세포는 후장과 직장에서 주로 많이 관찰되고, 점액세포의 점액과립이 분비된 후, 붕괴되는 과정에서 형성된 것으로 확인되었다.

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A palynological study of the genus Artemisia L. (Asteraceae) in Korea (한국산 쑥속(국화과)의 화분학적 연구)

  • Park, Myung Soon;Chung, Gyu Young
    • Korean Journal of Plant Taxonomy
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    • v.42 no.1
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    • pp.24-39
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    • 2012
  • The palynological study of 31 taxa of Korean Artemisia L. was carried out to evaluate their taxonomic characteristics and to determine the relationships among the taxa. The size and shape of the pollen are not used as characteristics to classify the taxa of Korean Artemisia L., but the granule distribution ands pinule connection on the exine were useful for an interspecific classification and for reflecting the relationship between some taxa. It is considered that Artemisia pollen has evolved from a connected spinule and a nearly psilate surface into 1) a connected spinule and a granule surface, 2) a disconnected spinule and a granule surface, 3) a disconnected spinule and a dense granule surface. These pollen characteristics with external morphological characteristics, a geographical distribution and a somatic chromosome number are considered to be good characteristics for distinguishing the relationship of some taxa, such as the A. japonica group (A. hallaisanensis, A. angustissima, A. littoricola) and the A. sacrorum group (A. sacrorum var. sacrorum, A. sacrorum var. incana, A. sacrorum var. iwayomogi).

Legumin Accumulation in Endoplasmic Reticulum Cisternae at Early Stage of Seed Development and Protein Body Transformation in Pea Cotyledon Cells (완두의 종자 발달과정에서 소포체 내강에 대한 저장 단백질 legumin의 축적과 단백과립 변환)

  • Jeong, Byung-Kap;Lee, Sun-Hee
    • Applied Microscopy
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    • v.31 no.4
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    • pp.347-354
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    • 2001
  • Immunoelectron microscopy of storage protein at early stage of seed development showed legumin was firstly accumulated protein in between endoplasmic reticulum (ER) cisternae, and these accumulates were differentiated into protein body (PB) by transformation at later stage. Thin sections of pea cotyledons during the later stages of seed maturation showed three morphologically different types of protein bodies. One of these, presented as rough-surfaced cisternae with terminal dilations, which contained protein deposits and were often found interdigitated between stacks of rough endoplasmic reticulum. Conventional electron microscopy at earlier stages of cotyledon development showed this protein body type initially developed from the rough ER. This transformation of endoplasmic reticulum into a protein body is believed to represent a new pathway of protein body development.

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The Ultrastructure of the Gastro-endocrine Cells in the Gastric Mucosa of the Frog, Rana rugosa (옴개구리 Rana rugosa 위점막의 위장관 내분비세포에 관한 전자현미경적 연구)

  • 이형식;이재현
    • The Korean Journal of Zoology
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    • v.33 no.1
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    • pp.22-27
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    • 1990
  • The gastro-endocrine cells were examined in the fundic region of stomach of the frog, Rana rugosa, by transmission electron microscope. In the present paper, at least three kinds of cell type distributed in this region were idendfted according to their morphological charactedstics based on the size, shape and electron density of the secretory granules. Type I cells were characterized by the presence of round or oval granules (300-5OOnm in diameter) with high electron density. The granules showed a wide lucent between the contents and the limiting membrane. This cell was reminiscent of the ECL cell in the human alimentary mucosa. Type II cells were characterized by the presence of spherical or oval granules (11O-230nm in diameter) with low or high electron density. The granules showed a clear halo between the homogenous contents and the limiting membrane. This cell was reminiscent of the G cell in the human alimentary mucosa. Type Ill cells were characterized by the presence of elongated oval or pheomorphic granules (50-2OOnm in diameter) with low or moderate electron density and abundant microfilament bundles in the cytoplasm. The granules contained a very thin halo.

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Morphological Study on the Mast Cell of Proventriculus in Pheasant (Phasianus colchicus) (꿩 전위의 비만세포에 관한 형태학적 연구)

  • Lee Y. H.
    • Korean Journal of Poultry Science
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    • v.32 no.2
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    • pp.97-100
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    • 2005
  • Mast cells have been studied extensively in various animals including rats and mice, whereas little is known the morphological data about pheasant mast cells. Here, morphological features of Korean pheasant mast cells are described in this study using light and electron microscopes. For light microscopy, mast cells had many metachromatic granules stained with toluidine blue in the cytoplasm. The fixation with $10\%$ neutral buffered formalin blocked staining of most mast cells but a modified Karnovsky solution proved to be a good fixative. In Korean pheasants, toluidine blue stained more mast cells than did alcian blue. For electron microscopy, the mast cells of the Korean pheasant were round, oval, spindle-like and irregular form and occasionally had a few short cytoplasmic processes. These cells had membrane-bounded granules and poorly developed organells. Some granules in the cytoplasm of the mast cells had bilayer membrane. Most granules were round shape and the membrane of several granules was concave or convex. The granules were composed of three parts, homogenous, particulate and reticular pattern.

Fine Structure of Oocyte Envelopes of Diploid and Triploid Biotypes in Cobitis hankugensis-Iksookimia longicorpa Complex (Cobitis hankugensis-Iksookimia longicorpa Complex의 2배체, 3배체집단의 난막 미세구조)

  • Ko, Meong-Hun;Park, Jong-Young
    • Korean Journal of Ichthyology
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    • v.22 no.1
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    • pp.56-60
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    • 2010
  • The Cobitis hankugensis-Iksookimia longicorpa complex from Korea has been presumed to be a unisexual lineage, originating from C. hankugensis and I. longicorpa and having almost all females. Recently, it was confirmed that the complex consisted of one diploid and two triploid complexes. From observation of their oocyte envelopes, three forms could be classified: a villous projection in I. longicorpa, a granular one in C. hankugensis and two triploid complexes, and a granule with villous one in the diploid complex. Even within the same granular projection, they showed specific features in length and density number from each other. These architectures are first observed in cobitid complexes and may playa role in identification of diploids and triploids.

Electron Microscopic Study on the Mast Cells of the Vertebrates(Mammals) Stomach (척추동물(脊椎動物)(포유류(哺乳類))위(胃)의 비만세포(肥滿細胞)에 관한 전자현미경적 연구)

  • Kang Ho-Suck;Kim Chang-Whan
    • Applied Microscopy
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    • v.11 no.1
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    • pp.39-50
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    • 1981
  • An electron microscopical observation was carried out to compare the general shape of the mast cells and structures of granules inside the cells in the stomach of 5 species in 3 orders of Mammals. In convenience, the granules in the cytoplasm were abbreviated as follows: 1) Homogeneous granule, GR1 2) Particulate granule, GR2 a. Dark dense particulate granule, GR2-1 b. Less dense particulate granule, GR2-2 3) Reticular granule, GR5 a. Dark dense reticular granule, GR5-1 b. Light dense reticular granule, GR5-2 In Mammalia including goat, dog, cat, and hamster, most of cytoplasmic organelle were Golgi apparatus and mitochondria, and most of the cytoplasmic granules were highly densed GR1and GR2. However GR5-1 and GR5-2 appeared in guinea pig while one side sunken or crescent-like types occured in both dog and guinea pig. All mast cells were oval or spindle with cytoplasmic processes around the cell. There was also found vacuoles and vesicles in these cells. These results demonstrated that there was a morphological difference between species of vertebrate in the mast cells and their cytoplasmic granules. It was also suggested that a variety of structures of granules were closely related with the composition (histamine, heparin, serotonin, hyaluronic acid etc.) and mature of the granules.

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Egg Membrane in Five Cobitid Species of Cobitis (Pisces : Cobitidae) (기름종개속 Cobitis(어강, 미꾸리과) 어류 5종의 난막)

  • Park, Jong-Young;Kim, Ik-Soo
    • Korean Journal of Ichthyology
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    • v.9 no.1
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    • pp.121-129
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    • 1997
  • The five species of genus Cobitis from Korea were investigated by electron microscopes to clarify the adhesive membranes on zona radiata. In the late vitellogenic stage the adhesive membranes could be classified into two form as follows: 1) granular form of Cobitis lutheri, C. striata, C. sinensis, and C. sinensis-longicorpus, 2) villous form of C. melanoleuca. Although C. lutheri, C. striata, C. sinensis, and C. sinensis-longicorpus possessed the same granular form, it was evident that fine structure of the zona radiata varied according to species. The adhesive membranes and fine structures of zona radiata in Cobitis showed a species specificity closely related to their habitats and spawning properties.

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Ultrastructure of the neuromuscular Junction in the Mantle Edge of the African Giant Snail, Achatina fulica (아프리카 왕달팽이(Achatina fulica) 외투막내 신경근연접의 미세구조)

  • 장남섭;한종민;황선종
    • The Korean Journal of Malacology
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    • v.14 no.2
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    • pp.75-83
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    • 1998
  • 아프리키 왕달팽이(Achatina fulica) 외투막(mantle edge) 내 근육 속의 신경근연접에 관한 미세구조 연구결과는 다음과 같았다. 신경근연접종말의 형태는 방추형이거나 불규칙형이었으며, 연접막과 근형질막상이 연접간극(synaptic cleft)의 넓이는 30mm 정도로 나타났다. 연접강속에는 전자밀도가 높은 둥근 과립(직경 100mm)과 중등도는 과립(직경 70mm)그리고 전자밀도가 낮은 소포(직경70mm)등 3종류가 관찰되었으며, 연접종말의 외측에는 0.2 - 0.4$\mu\textrm{m}$ 정도크기의 전자밀도가 높은 둥근 과립들이 존재하였다. 전자밀도가 중등도인 둥근 과립 중 일부는 연접막 밖으로 배출(exocytosis) 되었으며, 근형질막과 연접막이 합체되는 현상도 관찰되었다. 근형질(sarcoplasm)내에서 수축성단백질인 액틴과 미오신섬유의 배열은 불규칙하였으며, 세포질성 치밀체(cytoplasmic dense body)도 관찰되었다.

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