• Proceedings of the KIEE Conference
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• 2006.07b
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• pp.1073-1074
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• 2006

In this paper, we perform a simulation to verify the earth resistance values in Common Earth Network. The simulation is performed on the assumption that certain shorts are occurred in common earth network. Furthermore, from the result, we confirmed that very small earth resistance values in common earth network are given, by carrying out practical measurements in railway sections where common earth network is composed. From the effect, we could discover that the construction of common earth network is in a disadvantageous position on the financial aspect, while it is the most desirable way of construction for the purpose of Earth.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2010.04a
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• pp.150-153
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• 2010

본 논문에서는 비내진 설계된 철근콘크리트 골조로 이루어진 저층의 노후공동주택의 내진성능을 향상시키기 위한 구조물의 보강방법에 대해 연구하였다. 이를 위하여 비선형 정적 해석과 시간 이력 해석을 수행하여 추가되는 철골 모멘트골조와 가새의 내진보강 효과를 검증하였다. 해석결과에 따르면 $H150{\times}150{\times}6{\times}8$로 구성된 철골 모멘트골조는 탄성구간에서는 하중의 약 1%, 구조물이 항복한 이후, 최대 3.5%까지 하중을 부담하여 자체적으로 지진하중에 대한 저항 성능은 크지 않았다. 그러나 철골 모멘트골조와 가새를 동시에 사용함으로써 접합부의 조기 파괴를 방지하고 구조물의 내진성능을 큰 폭으로 증진시킬 수 있는 것으로 나타났다.

• Bulletin of the Society of Naval Architects of Korea
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• v.39 no.2
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• pp.75-82
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• 2002

잠수함 설계는, 내압(내압)성능 유지를 위해 효과적인 공간 활용이 어려운 원통형의 구조를 가질 뿐 아니라 제한된 공간에 다양한 장비들을 배치해야 하고,수 심 수백 미터의 극한 환경에서 3차원 운동을 하는 등의 환경 및 운용특성으로 인하여 수상 선박에 비해 많은 경험과 고난도의 기술들이 요구된다. 본문에서는, 해외의 유수한 대학 및 설계 기관과의 공동설계를 수행한 경험과 13년여에 걸친 장보고급 잠수함 건조기간 중에 수행한 수 차례의 역설계 과정 등을 통하여 습득한 설계 기술과 자료들을 바탕으로, 대우조선해양에서 자체 개발한 잠수함 기본설계 시스템(SUB-21)을 이용하여 수행한 중형잠수함 설계결과에 대해 간략하게 소개하고자 한다. 본 SUB-21의 개발은 잠수함 기본설계의 핵심 과정인 배치, 선형개발, 저항, 추진, 조종 및 압력선체 설계뿐만 아니라 디젤발전기-축전지-추진전동기로 구성되는 추진계통의 최적화 과정을 실시간으로 처리할 수 있기 때문에 기본설계에 소요되는 시간을 획기적으로 절감할 수 있으며 기본설계 결과의 신뢰성 제고에 크게 기여할 수 있을 것이다.

• Korean Journal of Heritage: History & Science
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• v.48 no.4
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• pp.74-93
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• 2015

Geophysical survey of Korea was introduced in Nara National Research Institute of Cultural Heritage in 1995. At that time, it has been activated geophysical survey of architecture and civil engineering in Korea. But there was no exploration experts to be combined the archaeology. For this reason, National Research Institute of Cultural Heritage has introduced the physical exploration. Through the expert exchanges South Korea and Japan carried out joint exploration. And it has increased the reliability of the exploration method and exploration results. It is GPR the most method commonly in geophysical exploration. There are many usability before excavation because of good resolution. However, the shallow GPR penetration depth has limitations in large mounds. We were able to take advantage of the resistivity analysis program to study the underground structure to deep through the experts exchange. We was able to get a good result that overcomes the limitations of GPR exploration in a number of burial mounds including Naju bokamri by the resistivity analysis program. In particular, we confirmed the location of the burial main body by compares the results of exploration and excavation results. In the future we will perform a convergence research of exploration and archaeology through a variety of joint research. In addition we will have to build a new network of archaeological science.

• Journal of the Korean Geotechnical Society
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• v.32 no.7
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• pp.25-34
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• 2016

Recently, in Korea, problems related with unstability of slope or sinkhole in urban area due to erosion of compacted granite soil which was used as a backfill or embankment material have been treated as important issues. Small hole might develop inside of backfill area due to erosion of not only weathered granite soil but also clay, silt, fine sand size particles when underground water flows. Once erosion starts in a soil mass, erosion rate increases gradually to cause rapid destruction. In this study, a rotating cylinder test (RCT) was performed to evaluate the hydraulic resistance characteristics of compacted weathered granite soil under various relative densities and preconsolidation pressures. Meanwhile, an image analysis method was introduced to analyze radius of irregularly eroded sample. It was found that image analysis is an effective means of minimizing the error in calculating a critical shear stress and threshold shear stress on the irregularly eroded sample. Furthermore, in general, hydraulic resistance capacity increases with the increase of relative density and preconsolidation pressure.

• Journal of Plant Biotechnology
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• v.35 no.1
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• pp.55-61
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• 2008

Previous studies on genetic transformation of chrysanthemum using cold regulated gene (BN115) have been conducted and the PCR and Real-Time PCR based method to determine the presence of the transferred cold regulated gene in the chrysanthemum was established. To check whether over-expression of BN115 gene in transgenic chrysanthemum will enhance their tolerance to cold stress, the transgenic chrysanthemum were grown under low temperature condition and several cold signalling including growth characteristics, stoma size and shape, SPAD value and ion leakage test were investigated. The transgenic chrysanthemum in the low temperature growth chamber grow much faster in term of the height, number and size of the leaves than those of wild-type plants and damage of transgenic plant caused by the low temperature was much less than that of wild-type plants. The stoma type and size of transgenic plant leaves grown at $5^{\circ}C$ were much similar to of wild-type plant cultured on $25^{\circ}C$ It has been found that SPAD value of transgenic plants was much higher than those of wild-type, but the EC density being lower under low temperature condition.

• Journal of Plant Biotechnology
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• v.31 no.4
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• pp.267-271
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• 2004

Transformed sweetpotato (Ipomoea batatas (L.) Lam. cv. Yulmi) plants were developed from embryogenic calli following Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain EHA105/pCAMBIA2301 harboring genes for intron $\beta$-glucuronidase (GUS) and kanamycin resistance. Transient expression of GUS gene was found to be higher when embryogenic calli were co-cultivated with Agrobacterium for 2 days. The co-cultured embryogenic calli transferred to selective MS medium containing 1mg/L 2,4-D, 100mg/L kanamycin, and 400mg/L claforan. These embryogenic calli were subcultured to the same selection medium at 4 weeks interval. Kanamycin-resistant calli transferred to hormone-free MS medium with kanamycin gave rise to somatic embryos and then converted into plantlets in the same medium. Southern blot analysis confirmed that the GUS gene was inserted into the genome of the sweetpotato plants. A histochemical assay revealed that the GUS gene was preferentially expressed in the leaf, petiole, and vascular tissue and tip of root.

• Journal of Plant Biotechnology
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• v.38 no.3
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• pp.198-202
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• 2011

Agrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic cucumber. Cotyledonary-node explants of cucumber (Cucumis sativus L. cv., Eunsung) were co-cultivated with Agrobacterium strains (EHA101) containing the binary vector (pPZP211) carrying with CaMV 35S promoter-nptII gene as selectable marker gene and 35S promoter-DQ gene (unpublished data) as target gene. The average of transformation efficiency (4.01%) was obtained from three times experiments and the maximum efficiency was shown at 5.97%. A total of 9 putative transgenic plants resistant to paromomycin were produced from the cultures of cotyledonary-node explants on selection medium. Among them, 6 transgenic plants showed that the nptII gene integrated into each genome of cucumber by Southern blot analysis.

• Korean Journal of Weed Science
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• v.18 no.3
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• pp.205-213
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• 1998

This experiment was carried out to produce herbicide resistant potatoes hawing only chimeric phosphinothricin acetyltransferase (PAT) genes without using antibiotic selectable marker. The pDY502 vector having only PAT gene was reconstructed for transformation of potato. The reconstructed vector was introduced to Agrobacterium tumefaciens MP90 disarmed, and they were used for potato transformation. Hormonal requirement for plant regeneration from leaves and stem explants of potato was investigated. From this experiment, MS medium treated with IBA 0.1 mg/L + BA 0.5 mg/L was the best for potato regeneration, and the ratio of shoot regeneration was 54% for leaf and 46% for stem in that condition. For transformation, explants of potato leaves and stems were cocultured with A. tumefaciens MP90 containing reconstructed vector harvoring only PAT gene. When the potato explants were placed on various concentrations of bialaphos and all the potato explants were dead on medium with over 5.0mg/L bialaphos. By this selection methods, the explants cocultured with Agrobacterium produced the putative transgenic shoots on medium with 5mg/L bialaphos treatment after 3-4 weeks. Second selection was performed by transferring the shoot tips of putative transgenic to medium containing 20mg/L of bialaphos. The shoot tips grew well on the second selection medium, indicating the production of successful transgenic plants. But normal shoots were dead in same cytotoxic medium. Incorporation of the PAT gene into transgenic potatos were confirmed by PCR analysis of DNA and Southern hybridization. These results show that the PAT gene can serve as a selectable marker and herbicide resistant genes for transformation of potato.

• Microbiology and Biotechnology Letters
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• v.44 no.3
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• pp.341-348
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• 2016

This study aimed to develop a microbial process for producing aminolevulinic acid (ALA) using crude glycerol. In the culture of ALA-producing cells (Escherichia coli/pH-hemA) in a medium containing crude glycerol, the cell density and production were 1.8-fold and 1.2-fold lower than those obtained from pure glycerol, respectively. However, the cell growth and production were improved by supplementing the medium with trehalose (30 or 100 g/l). Engineered cells (E. coli/pH-hemA/pS-otsBA) were constructed to express otsBA and their culture performance was compared with that of control cells (E. coli/pH-hemA/ pSTV28). The effects of isopropyl β-D-1-thiogalactopyranoside (IPTG) concentration and the time of induction were examined to improve the cell growth and ALA production in engineered cells cultured using crude glycerol. When 0.6 mM of IPTG was added at the beginning of the exponential growth phase, the ALA produced by cells was 2,121 mg/l, which was comparable to that from pure glycerol. The results demonstrate that otsBA expression endowed cells with the capacity to tolerate the toxicity of crude glycerol for direct use.