• Title/Summary/Keyword: 고정벽

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Preparation and Photosensitivity of Ag-Multi Walled Carbon Nanotube-TiO2 Nano Composite (Ag-Multi walled carbon nanotube-TiO2 복합나노소재 제조 및 광감응성)

  • Kim, Sung-Pil;Kim, Jong-Oh
    • Journal of the Korean GEO-environmental Society
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    • v.17 no.2
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    • pp.5-11
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    • 2016
  • $MWCNT-TiO_2$ nano composites and $Ag-MWCNT-TiO_2$ nano composites were prepared from Multi-Walled Carbon NanoTube (MWCNT), titanium (IV) butoxide (TNB) solution and silver nitrate ($AgNO_3$) by the sol-gel method. The dispersion and structure of Ag in the synthesized composites was observed by Scanning Electron Microscopy (SEM) and Field Emission Transmission Electron Microscopy (FE-TEM). X-Ray Diffraction (XRD) patterns of the composites showed that the composites contained an anatase phase. The Energy Dispersive X-ray spectroscopy (EDX) showed the presence of C, O, Ti and Ag peaks. The $TiO_2$ particles were distributed uniformly in the MWCNT network, and Ag particles were virtually fixed on the surface of the tubes. Also decomposition of the methylene blue was investigated according to UV radiation times for study photocatalytic activity. $Ag-MWCNT-TiO_2$ nano composites show high photodegradation than $MWCNT-TiO_2$ nano composites. The results indicate that the high conductivity of Ag improved the photoactivity of the $MWCNT-TiO_2$ composite.

Factors Influencing Protoplast Isolation and Culture in Three Capsicum Species (세 가지 고추속 식물의 원형질체 분리 및 배양에 미치는 요인)

  • 임학태;염옥희;전익조;조미애;양승균
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.3
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    • pp.141-146
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    • 2001
  • Protoplasts were isolated from cotyledons, hypocotyls, and mesophyll tissues of three species of Capsicum species (C. anuumm, C. bacatuum, and C. chacoense). Combination of Cellulysin (1%) and Macero-zyme (0.25%) in 0.65 M sorbitol was found to be the most effective for the digestion of cell wall, regardless of the Capsicum species. Antioxidant MES (2-[N-Morpholino]ethanesulfonic acid) in the enzyme solution helped protoplasts overcome browning. After 5 days of initial culture, Cell division occurred in modified K8p medium containing 1~5 mg/L zeatin, 0.5 mg/L IAA, 0.1~0.5 mg/L TDZ, and 1 mg/L 2,4-D under continuous dark condition at $25^{\circ}C$. Semi-solid agarose culture method was more effective than liquid culture, and it also protected the cells from browning caused by polyphenolic compound released during protoplast culture. A total of 4000 calli were obtained from protoplast culture of different capsicum species. All of these calli were transferred to the 100 combinations of regeneration media using various plant growth regulators; TDZ, IAA, 2ip, BAP, NAA, and zeatin. These calli derived from protoplast of three species of capsicum were, however, not differentiated into shoots.

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Enhancement of Antioxidant Activities of Blueberry (Vaccinium ashei) by Using High-Pressure Extraction Process (초고압 처리가 블루베리의 항산화 증진에 미치는 영향)

  • Park, Sung Jin;Choi, Young Bum;Ko, Jung Rim;Kim, Young Eon;Lee, Hyeon Yong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.3
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    • pp.471-476
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    • 2014
  • We developed a method for improving the antioxidant activities of blueberry (Vaccinium ashei) extracts through an ultra high-pressure extraction process. Blueberries were subjected to water extraction at $60^{\circ}C$ and 300 MPa for 5 min (High Pressure Extraction, HPE5) and 15 min (HPE15). Extraction yields obtained by ultra high-pressure extraction process were 18.48, and 19.89%, respectively. Total polyphenol contents were estimated to be 28.3, and 28.9 mg/g, whereas flavonoid contents were measured as 5.9 and 6.0 mg/g, respectively. Generally, HPE resulted in higher yields than the conventional extraction process. Further, HPE15 showed 53.84% DPPH radical scavenging activity (EDA, %) at $1,000{\mu}g/mL$. Reducing power of HPE15 showed its highest activity of 0.21. In general, antioxidant activities of blueberry increased by HPE. Therefore, HPE of blueberry resulted in higher antioxidant activity than conventional water extraction. These results demonstrate obvious advantages in terms of higher efficiency, shorter extraction time, and lower energy costs.

Shape Oscillation and Detachment of Droplet on Vibrating Flat Surface (진동하는 평판 위의 액적의 형상 진동 및 제거 조건에 대한 연구)

  • Shin, Young-Sub;Lim, Hee-Chang
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.38 no.4
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    • pp.337-346
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    • 2014
  • This study aimed to understand the mode characteristics of a droplet subject to periodic forced vibration and the detachment of a droplet placed on a plate surface. An surface was coated with Teflon to clearly observe the behavior of a droplet. The contact angle between the droplet and surface and the hysteresis were found to be approximately $115^{\circ}C$ and within $25^{\circ}C$, respectively. The coating process was performed in a clean room that had an environment with a low level of contaminants and impurities such as air dust, detergents, and particles. To predict the resonance frequency of a droplet, theoretical and experimental approaches were applied. Two high-speed cameras were configured to acquire side and top views and thus capture different characteristics of a droplet: the mode shape, the detachment, the separated secondary droplet, and the waggling motion. A comparison of the theoretical and experimental results shows no more than 18 discrepancies when predicting the resonance frequency. These differences seem to be caused by contact line friction, nonlinear wall adhesion, and the uncertainty of the experiment. For lower energy inputs, the contact line of the droplet was pinned and the oscillation pattern was axisymmetric. However, the contact line of the droplet was de-pinned as the oscillation became more vigorous with increased energy input. The size of each lobe at the resonance frequency is somewhat larger than that at the neighboring frequency. A droplet in mode 2, one of the primary mode frequencies, exhibits vertical periodic movement as well as detachment and secondary ejection from the main droplet.

In Vivo Study on the Biocompatibility of New Resin-based Root Canal Sealers (신개발 레진 계열 봉함제의 생체친화성에 관한 연구)

  • Kim, Yong-Beom;Baek, Seung-Ho;Bae, Kwang-Shik
    • Restorative Dentistry and Endodontics
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    • v.27 no.2
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    • pp.122-134
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    • 2002
  • 목적 : 근관 치료의 최종 목적인 근관계의 영구적인 충전을 위해 사용되는 근관 봉함제는 많은 연구와 개선을 거쳐서 현재는 다양한 성분의 봉함제가 시판되고 있다. 이중에서 레진이 주성분인 봉함제는 조작이 편리하고 흐름성이 좋으며 근관의 벽에 높은 밀폐성을 보이고 충분한 작업시간과 높은 방사선 불투과성을 가지는 장점을 가짐에도 불구하고 높은 초기 생체 독성을 나타내는 단점을 가지고 있다. 본 실험에서는 기존의 상용화된 제품 중 레진이 주성분인 두 종류의 봉함제(AH 26, AH plus)와 산화아연이 주성분인 봉함제(Pulp Canal Sealer EWT)와 국내에서 새로이 개발한 제품으로서 레진이 주성분인 두 종류의 봉함제(Adseal-1,2)를 생체조직에 매식하여 국소적인 반응을 비교하여 생체친화성을 알아보고자 하였다. 방법 : 수종 봉함제의 생체 친화성을 알아보기 위하여 64마리의 Sprague-Dawley rat을 사용하였다. 봉함제의 피하조직 매식을 위해 길이와 직경이 각각 5와 1.5mm인 폴리에틸렌 테프론 관을 사용하였으며 이를 에탄올과 증류수로 세척 한 후 고압증기멸균을 시행하였다. Rat에 대하여 케타민으로 복강내 마취를 시행한 후 배부를 면도하고 iodine으로 소독한 다음 네 곳에 절개를 시행하였으며 blunt dissection을 통해 깊이 10mm이상의 피하조직 pocket을 형성하였다. 각각의 봉함제를 제조사의 지시 에 따라 혼합 후 즉시 멸균된 테프론 관에 주사기를 이용하여 담은 다음에 봉함제가 흐르지 않게 유의하며 pocket내로 삽입하였으며 이때 16개의 관을 대조군으로 사용하기 위해 봉함제를 넣지 않은 상태로 삽입하였다. 이 후 절개 부위를 surgical gut suture로 봉합하였으며 1주일 후에 발사하였다. Rat을 1, 2, 4, 12주 후에 각 군 당 세 마리 씩 에테르 흡입을 통해 희생하였으며 이 때 한 마리씩의 대조군도 포함시켰다. 이 후 매식된 관을 주위 조직과 함께 제거하고 포르말린에서 48시간 고정시킨 후 파라핀에 포매한 다음에 micro-tome을 사용하여 6$\mu\textrm{m}$로 serial section을 시행하였다. 정중선 부위의 시편에 Hematoxylin-Eosin staining을 시행한 후 Olsson, Orstavik 그리고 Mjor 등의 방법에 따라 조직학적 변화를 관찰한 후 slight(1), moderate(2), severe inflammation(3)의 단계로 분류하였다. 얻어진 결과를 통계처리 프로그램인 Jandel사의 Sigmastat을 이용하여 Kruskal Wallis Test로 통계처리를 하였다. 결과 : (Table omitted) 결론 : 1) Pulp Canal Sealer를 제외한 모든 군에서 시간이 지남에 따라 유의성 있게 염증이 감소되는 양상을 보였다(p<0.05). 2) Pulp Canal Sealer는 1주, 2주, 12주에서 강한 염증반응을 보였다. 3) AH 26과 AH Plus에서는 1주, 2주에서 강한 염증반응을 보였으나 12주에서는 염증반응이 감소하였다. 4) 새로 개발된 봉함제 Adseal-1,2는 1주, 2주에서는 가장 약한 염증반응을 보이나 4주, 12주 후에는 AH Plus와 비슷한 수준의 염증 반응을 보였다. 5) Pulp Canal Sealer를 제외한 모든 군에서 인정할 만한 생체친화성을 보였다. 6) Adseal-2가 Adseal-1에 비하여 전반적으로 낮은 염증반응을 보였다. 7) 각 군간 결과의 차이에 통계적 유의성은 없었다(p>0.05).

A Study on the Surface-Radiation Heat Transfer Characteristics in an Open Cavity with a Heat Source (발열체가 존재하는 개방된 정사각형공간에서 표면복사 열전달 특성에 관한 연구)

  • Nam, Pyoung-Woo;Park, Myoung-Sig;Park, Chan-Woo
    • Solar Energy
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    • v.12 no.3
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    • pp.70-83
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    • 1992
  • The interaction between the surface radiation and the mixed convection transport from an isolated thermal source, with a uniform surface heat flux input and located in a rectangular enclosure, is stuied numerically. The enclosure simulates a practical system such an air cooled electric device, where an air-stream flows through the openings on the two vertical walls. The heat source represents an electric component located in such an enclosure. The size of this cavity is $0.1[m]{\times}0.1[m]$. The inlet velocity is assumed as 0.07[m/s] and the inlet temperature is maintained as $27^{\circ}C$. The inflow is kept at a fixed position. Laminar, two dimensional flow is assumed, and the problem lies in the mixed convection regime, governed by buoyancy force and surface readiation. The significant variables include the location of the out-flow opening, of the heat source and the wall emissivity. The basic nature of the resulting interaction betwwn the externally induced air stream and the buoyancy-driven flow generated by the source is investigated. As a result, the best location of the heat source to make the active heat transfer is 0.075[m] from the left wall on the floor. The trends observed are also discussed in terms of heat removal from practical systems such as electric circuitry.

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Numerical Analysis of the Complex Permittivity of MWNT added Epoxy Depending on Agglomeration Size (에폭시 내부의 MWNT 응집 크기에 따른 복소유전율 변화의 해석적 관찰)

  • Shin, Jae-Hwan;Jang, Hong-Kyu;Choi, Won-Ho;Song, Tae-Hoon;Kim, Chun-Gon;Lee, Woo-Yong
    • Composites Research
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    • v.27 no.5
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    • pp.190-195
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    • 2014
  • This paper predicts the complex permittivity of MWNT added epoxy depending on agglomeration by numerical analysis. 1wt% MWNT added epoxy specimen is prepared using 3-roll-mill method and its complex permittivity is measured in X-band (8.2~12.4 GHz) using freespace measurement system. The analytic model is comprised of cube epoxy and perfect sphere agglomeration. The complex permittivity of the agglomeration model is predicted by complex permittivity mixing rule using the measured complex permittivity of epoxy and 1 wt% MWNT added epoxy. Commercial electromagnetic analysis software, CST, is used to obtain S-parameter of the analytic model and MATLAB code is used to calculate complex permittivity from the S-parameter. It is confirmed that the complex permittivity increases when the agglomeration size decreases.

Quality Characteristics of Citrus Fruit by Cyclic Low Pressure Drying and High Hydrostatic Pressure Extraction (초고압 처리에 의한 감귤의 추출률 및 특성변화)

  • Park, Sung-Jin;Choi, Young-Bum;Ko, Jung-Rim;Rha, Young-Ah;Lee, Hyeon-Yong
    • Culinary science and hospitality research
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    • v.20 no.3
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    • pp.13-21
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    • 2014
  • We developed a method for improving the antioxidant activities of Citrus extracts through cyclic low pressure drying(CLPD) and a high hydrostatic pressure extraction (HPE) process. Citrus fruits were prepared for water extraction at $60^{\circ}C$ and 300 MPa for 5 min (high hydrostatic pressure extraction, HPE5) and 15 min (high hydrostatic pressure extraction, HPE15) after cyclic low pressure drying method. Extraction yields obtained by cyclic low pressure drying and high hydrostatic pressure extraction process were 20.41, 23.47, and 28.19%, respectively. Total polyphenol contents were increased by combined process. Generally, CLPD and HPE resulted in higher yields than the conventional extraction process. Further, HPE15 showed 48.21% DPPH radical scavenging activity (EDA, %) at $1,000{\mu}g/mL$. In general, antioxidant activities of Citrus increased by CLPD and HPE. Therfore, CLPD and HPE of Citrus resulted in higher antioxidant activity than conventional water extraction.

Volatile Compounds and Ultrastructure of Petal Epidermal Cells According to Scent Intensity in Rosa hybrida (장미꽃의 향기정도에 따른 주요성분 및 꽃잎 세포의 미세구조)

  • Lee, Young-Soon;Lee, Yun-Hae;Lim, Seung-Hee;Park, Gun-Hwan;Choi, Sunk-Young;Hong, Hae-Jung;Ko, Jeong-Ae
    • Horticultural Science & Technology
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    • v.31 no.5
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    • pp.590-597
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    • 2013
  • Principle component and emission localization of volatile compounds were investigated according to scent intensity of rose flower. Scent intensity in cultivars and bred-line of Rosa hybrida was divided into three levels; light ('Feel Lip', 'Venus Berry'), medium ('GR07-135'), strong ('Honey Blue'). The major volatile compounds were different depending on the cultivars and selected line; 3,5-dimethoxytoluene (DMT), benzene, 1,3,5-trimethoxy ('Feel Lip'), megastigma-4,6(Z),8(E)-triene ('Venus Berry'), DMT, benzene,1-ethenyl-4-methoxyand, phenylethylalcohol ('GR07-135') and germacrene-D, DMT ('Honey Blue'). The adaxial epidermal cells were conical papillate shape, whereas the abaxial epidermal cells were flat shape. The adaxial epidermal cells of 3 cultivars and 1 selected line were surrounded by thick cell wall and covered by waxy cuticle of 2 cultivars and 1 selected line (except 'Honey Blue'). The adaxial epidermal cells contained starches in 'Feel Lip', osmiophlic droplets in 'Venus Berry', starchs, plastids, vacuoles in 'GR07-135' and plastoglobules, plastids, vacuoles in 'Honey Blue'. Based on these results, it appears that plastids and vacuoles in adaxial epidermal cells with conical papillate shape are associated production and emission of volatile compounds in scent R. hybrida.

Characterization of Potential Plant Growth-promoting Rhizobacteria as Biological Agents with Antifungal Activity, Plant Growth-promoting Activity, and Mineral Solubilizing Activity (항진균 활성, 식물 생장촉진 활성, 미네랄 가용화능을 가진 생물학적 제제로서 잠재적 식물 생장촉진 근권세균의 특성조사)

  • Lee, Song Min;Kim, Ji-Youn;Kim, Hee Sook;Oh, Ka-Yoon;Lee, Kwang Hui;Lee, Sang-Hyeon;Jang, Jeong Su
    • Journal of Life Science
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    • v.31 no.7
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    • pp.641-653
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    • 2021
  • The purpose of this study was to confirm the antifungal activity, plant growth-promoting activity, and mineral solubilizing activity of 18 types of bacteria isolated purely from rhizosphere soil. The potential of isolates of the genus Bacillus and Pseudomonas as biocontrol agents was confirmed through the antifungal activity of these isolates. This activity has been determined to be due to various hydrolytic enzymes on the cell wall of plant pathogenic fungi and the production of siderophores in isolates. In addition, most of the isolates have been found to have aminocyclopropane-1-carboxylate deaminase production activity, indole-3-acetic acid production activity, and nitrogen fixation activity. These characteristics are believed to have a positive effect on root development, growth, and the productivity of crops via a reduction in the concentration of ethylene under conditions of environmental stress, to which plants are commonly exposed. In addition, on testing for the solubilizing activity of the isolates for phosphoric acid, silicon, calcium carbonate, and zinc, some isolates were found to have mineral solubilizing activities. Inoculation of these isolates during plant growth is expected to assist plant growth by converting nutrients necessary for growth into usable forms that can be absorbed by plants. The 18 isolated strains can be used as biocontrol agents due to their antifungal activity, plant growthpromoting activity, and mineral solubilizing activity.