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Peptide Profiling and Selection of Specific-Expressed Peptides in Hypoglycemic Sorghum Seed using SELDI-TOF MS (SELDI-TOF MS를 활용한 혈당강하 수수 종자의 펩타이드 프로파일링 및 특이 발현 펩타이드 선발)

  • Park, Sei Joon;Hwang, Su Min;Park, Jun Young;Ko, Jee-Yeon;Kim, Tae Wan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.3
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    • pp.252-262
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    • 2014
  • Sorghum seed is traditionally used as secondary food sources in addition to rice in Korea. While the hypoglycemia regulating phytochemicals have been found in sorghum seed, peptides related with hypoglycemia never been studied before. To obtain the peptide characteristics and the specifically high-expressed peptides in hypoglycemic sorghum seed, peptide profiles of seven hypoglycemic and five non-hypoglycemic sorghum lines bred in RDA were determined using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). The twelve sorghum lines exhibited 104 peptides on CM10 protein chip array (weak cation exchange) and 95 peptides on Q10 (weak cation exchange) in the molecular mass range from 2,000 to 20,000 Da. Heat map via supervised hierarchical clustering of the significantly different peptides (p < 0.01) in peak intensity among the 12 lines effectively revealed the specifically upregulated peptides in each line and distinguished between 7 hypoglycemic and 5 non-hypoglycemic lines. Through the comparison with hypoglycemic and non-hypoglycemic lines, 10 peptides including 2231.6, 2845.4, 2907.9, 3063.5, 3132.6, 3520.8, 4078.8, 5066.2, 5296.5, 5375.5 Da were specifically high-expressed in hypoglycemic lines at p < 0.00001. This study characterized seed peptides of 12 sorghums and found ten peptides highly expressed for hypoglycemic sorghum lines, which could be used as peptide biomarkers for identification of hypoglycemic sorghum.

A Study to Improve the Spatial Data Design of Korean Reach File to Support TMDL Works (TMDL 업무 지원을 위한 Korean Reach File 공간자료 설계 개선 연구)

  • Lee, Chol Young;Kim, Kye Hyun;Park, Yong Gil;Lee, Hyuk
    • Journal of Korea Water Resources Association
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    • v.46 no.4
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    • pp.345-359
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    • 2013
  • In order to manage water quality efficiently and systematically through TMDL (Total Maximum Daily Load), the demand for the construction of spatial data for stream networks has increased for use with GIS-based water quality modeling, data management and spatial analysis. The objective of this study was to present an improved KRF (Korean Reach File) design as framework data for domestic stream networks to be used for various purposes in relation to the TMDL. In order to achieve this goal, the US EPA's RF (River Reach File) was initially reviewed. The improved design of the graphic and attribute data for the KRF based on the design of the EPA's RF was presented. To verify the results, the KRF was created for the Han River Basin. In total, 2,047 stream reaches were divided and the relevant nodes were generated at 2,048 points in the study area. The unique identifiers for each spatial object were input into the KRF without redundancy. This approach can serve as a means of linking the KRF with related database. Also, the enhanced topological information was included as attributes of the KRF. Therefore, the KRF can be used in conjunction with various types of network analysis. The utilization of KRF for water quality modeling, data management and spatial analysis as they pertain to the applicability of the TMDL should be conducted.

GIS spatial D/B formation of geothermal data and Distribution of Heat Flow of Korea (한국의 지열자료 GIS 공간 D/B 구축과 지열류량 분포)

  • Kim, Hyoung-Chan;Lee, Young-Min;Park, Jeong-Min
    • 한국신재생에너지학회:학술대회논문집
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    • 2006.06a
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    • pp.459-460
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    • 2006
  • 현재 남한의 지열류량 측정값으로는 총 363개 지점의 자료가 측정 및 수집되어 있다. 이것은 Mizutani et at. (1970), 장정진 외(1970), 그리고 서정희(1976) 등의 자료, 총 35개의 자료도 추가된 것이다. 1989년 이후부터 측정된 지열류량 자료는 217개 자료이며(임정웅 외, 1989; 임정웅 외, 1996; Lim and Kim, 1997; 염병우 외, 1997), 모두 직접 측정한 것이나, 1989년 이후 보고된 지열류량 자료에 약간의 오류가 있어 이번 연구에서 수정 보완하였다. 또한 과거의 자료 35개 자료는 이미 지열류량 측정치가 논문화 되어 있는 것으로 암석시료는 없다 1989년 이후 2004년까지 자료 217개 2005년도 추가 자료 111개의 지열류량 자료는 암석시료도 있으며, 측정기기가 서로 달라 오차가 있을 수 있어 서로 보정을 해야 할 필요가 있어 시추공 주변 암석을 새로 수집해서 신장비로 다시 측정 보정하였다. 지열류량 D/B 구축은 각 자료의 일련번호, 고유번호 (Sn.), 위경도 좌표 (longitude, lattitude), 암석의 열전도도(thermal conductivity), 지온경사 (thermal gradient), 지열류량 (heat flow)등으로 구성되어 있다. 지열류량 자료 공간 데이터베이스는 점 속성을 가지며 자료형태는 각종 소프트웨어와 호환성이 좋은 shape 파일 형태로 작성하였다. 또만 최근 천부 토양 및 암석 열물성을 이용한 냉난방시스템 즉, Heat Pump System 설계를 위하여 반드시 들어가야 하는 요소인 열확산율, 공극율, 밀도, 비열 등 열물성 특성을 추가하여 GIS 공간 D/B구축하였다. 대륙붕 자료 4개 자료를 제외하고 359개의 지열류량 자료를 이용하여 한반도 남부, 즉 남한의 지열류량 분포도를 작성 분석해 본 결과(그림 1), 우리나라의 지열류량 이상대는 아산만 주변, 보령, 유성, 진안, 울진, 포항, 부산 지역과 포천, 속초, 충주, 수안보 등 지역에서 나타난다 이러한 이상대 주변에는 대개 온천이 발달되어 있었거나 새로 개발되어 있는 곳이다. 온천에 이용하고 있는 시추공의 자료는 배제하였으나 온천이응으로 직접적으로 영향을 받지 않은 시추공의 자료는 사용하였다 이러한 온천 주변 지역이라 하더라도 실제는 온천의 pumping 으로 인한 대류현상으로 주변 일대의 온도를 올려놓았기 때문에 비교적 높은 지열류량 값을 보인다. 한편 한반도 남동부 일대는 이번 추가된 자료에 의해 새로운 지열류량 분포 변화가 나타났다 강원 북부 오색온천지역 부근에서 높은 지열류량 분포를 보이며 또한 우리나라 대단층 중의 하나인 양산단층과 같은 방향으로 발달한 밀양단층, 모량단층, 동래단층 등 주변부로 NNE-SSW 방향의 지열류량 이상대가 발달한다. 이것으로 볼 때 지열류량은 지질구조와 무관하지 않음을 파악할 수 있다. 특히 이러한 단층대 주변은 지열수의 순환이 깊은 심도까지 가능하므로 이러한 대류현상으로 지표부근까지 높은 지온 전달이 되어 나타나는 것으로 판단된다.

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Designing a Web-Based Jeongganbo Notation Program (웹기반 정간보 사보 프로그램 설계)

  • Shim, In Sup
    • The Journal of the Korea Contents Association
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    • v.22 no.4
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    • pp.742-753
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    • 2022
  • Recently, the globalization of culture and the global trend of K-culture have become a good background for further developing and promoting Korean traditional culture. However, although Korean traditional music has an excellent notation method called Jeongganbo developed by King Sejong, it is gradually not being used as the universality of Western notation. However, since the 7th curriculum, the proportion of introduction and utilization of Jeongganbo in elementary music education has been increasing. And this is a good opportunity to re-generalize our unique traditional notation. On the other hand, in addition to practicing the interpretation of the notation method, more diverse tools will be needed to activate the practice of Jeongganbo. Therefore, this study studied the design of a web program that can make Jeongganbo on the web without installation or download. In addition, in order to increase the user's convenience in the process of publishing Jeongganbo, it designs and proposes a method of inputting using a computer keyboard like a piano keyboard and method of listening to input music information through MIDI function and save it as a file. Through this study, it is possible to develop a web program that anyone can easily and simply approach, practice, and publish, and it is expected to be a useful tool for practicing Korean traditional music or publishing traditional music in the future.

A Study on the Characteristics of Non-Fungible Token(NFT) and Application Plans from the Digital Records Perspective : Focused on Transferable Records (전자기록 관점에서 본 대체 불가능한 토큰(NFT) 특성 및 활용 방안 이전 및 거래 가능한 기록을 중심으로)

  • Won, Joo-hye;So, Hyeon-Gi;Oh, Hyo-Jung
    • The Korean Journal of Archival Studies
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    • no.73
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    • pp.47-79
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    • 2022
  • NFT is literally a 'non-fungible token', a digital file that records specific virtual assets on a blockchain. Events such as ownership of the asset and transaction history are recorded on the blockchain through the token transaction, so counterfeiting and falsification are impossible. Therefore, NFT is used as a tool that can uniquely represent a specific virtual asset. The main purpose of this paper is to examine the characteristics of NFT from a records management point of view and to find ways to use them, and focuses on digital records that have the characteristics of assets as digital works. For this purpose, we first examine the basic concept of NFT and the principle of ownership and proof of value as an asset for digital works. In addition, it was confirmed how the advantages of NFT were applied through NFT use cases in various fields, and in particular, areas related to audio-visual records such as art, music, sports, and fashion were focused on. Furthermore, by comparing the characteristics of digital records with those of NFT, factors applicable to electronic records were identified. Finally, the types of digital records that are expected to be effective in the application of NFT were identified, and the possibility of their use and discussion points for introduction in records management are presented.

The Study of Discriminant Validity for the Sensory Processing Scale for Children (SPS-C) of Children with and without Sensory Processing Disorder (아동감각처리척도(Sensory Processing Scale for Children; SPS-C)의 판별타당도 연구)

  • Kim, Kyeong-Mi
    • The Journal of Korean Academy of Sensory Integration
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    • v.20 no.2
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    • pp.36-45
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    • 2022
  • Objective : The objective of this study was to determine a discriminat validity of the Sensory Processing Scale-Children (SPS-C) for children with and without Sensory Processing Disorder (SPD). Method : The SPS-C was completed by parents of 151 Korean children including 68 typically developing children (control group), 83 children with SPD (known group) in the age range 3-5 years, in South Korea. ANCOVA with sex as covariable was used to identified the difference between the children with typically development and SPD. Results : Sensory processing differences in sensory domains and sensory factors were detected and the gender differences were not effective between typically developing children and children with SPD. Significant differences were found in all sensory domain score and total score except proprioceptive processing in sensory domain. In sensory factors, significant differences were found in overrsensitivity, underresponsivity, and discrimination, but sensory seeking was not significant. SPS-C was a valid assessment tool to identify or screen of SPD in Korea. Conclusion : The psychometric characteristics for the standardization study of SPS-C were presented, and the discriminant validity to screen the children with sensory processing problems was presented as a verified evaluation tool. SPS-C will be helpful in screening, analyzing and interpreting characteristics of sensory processing, and establishing an intervention plan.

A Study on Non-Fungible Token Platform for Usability and Privacy Improvement (사용성 및 프라이버시 개선을 위한 NFT 플랫폼 연구)

  • Kang, Myung Joe;Kim, Mi Hui
    • KIPS Transactions on Computer and Communication Systems
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    • v.11 no.11
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    • pp.403-410
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    • 2022
  • Non-Fungible Tokens (NFTs) created on the basis of blockchain have their own unique value, so they cannot be forged or exchanged with other tokens or coins. Using these characteristics, NFTs can be issued to digital assets such as images, videos, artworks, game characters, and items to claim ownership of digital assets among many users and objects in cyberspace, as well as proving the original. However, interest in NFTs exploded from the beginning of 2020, causing a lot of load on the blockchain network, and as a result, users are experiencing problems such as delays in computational processing or very large fees in the mining process. Additionally, all actions of users are stored in the blockchain, and digital assets are stored in a blockchain-based distributed file storage system, which may unnecessarily expose the personal information of users who do not want to identify themselves on the Internet. In this paper, we propose an NFT platform using cloud computing, access gate, conversion table, and cloud ID to improve usability and privacy problems that occur in existing system. For performance comparison between local and cloud systems, we measured the gas used for smart contract deployment and NFT-issued transaction. As a result, even though the cloud system used the same experimental environment and parameters, it saved about 3.75% of gas for smart contract deployment and about 4.6% for NFT-generated transaction, confirming that the cloud system can handle computations more efficiently than the local system.

Structure of the Temperature and Salinity in 2003-2005 Profiled by the ARGO floats around the Ulleung-do area in the East Sea (ARGO 뜰개에 의한 2003-2005년 울릉도 주변 해역의 수온-염분 구조)

  • Kim, Eung;Ro, Young-Jae;Youn, Yong-Hun
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.11 no.1
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    • pp.21-30
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    • 2006
  • This study investigated the temperature-salinity spatio-temporal variability around the Ulleung-do Island (UI) by using CTD profiles obtained by the ARGO floats far the period of Oct.,2003 to Aug.,2005. The waterbody in the upper 700 m around the UI could be classified into five water masses, which is consistent to traditional water characteristics in the East Sea. In the upper surface layer, the temperature and salinity in fall season became even lower than those properties in the summer time. The East Sea Intermediate Water (ESIW) characterized by the salinity minimum layer shows the range of potential temperature between 1 to $5^{\circ}C$ and salinity lower than 34.06 psu. The ESIW lies approximately at 265 m depth with average thickness of 175 m. This thickness of the ESIW continues to be relatively uniform regardless of spatio-temporal space. However, the depth of the ESIW shows vertical variation influenced by the Ulleung warm eddy (UWE). Since the UWE lies in the upper layer, the Upper Portion of the Japan Sea Proper. Water (UPJSPW) is also affected to show the vertical variation. The influence extorted by the UWE reached down to 700 m depth in terms of temperature. The CTD profiles obtained with the high sampling rate by ARCO floats over two-year period provided with very useful and detailed informations in investigating the spatio-temporal variability In the study area.

Microarray profile of hypothalamic gene expression with acupuncture at acupoint ST36 in carrageenan induced inflammation in Stat 6 knockout mice (Carrageenan으로 염증을 유도한 Stat 6 유전자제거 생쥐의 족삼리 침치료에 대한 시상하부 유전자의 마이크로어레이 프로파일)

  • Park, Hi-Joon;Um, Yoon-Kyung;Jung, Kyung-Hee;Kim, Soo-Cheol;Han, Mi-Young;Hong, Mee-Suk
    • Korean Journal of Acupuncture
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    • v.24 no.2
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    • pp.129-139
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    • 2007
  • 목적 : Signal transducers and activators of transcription 6 (Stat 6) 유전자는 면역세포의 발달에 있어서 중요한 유전인자이며, IL-4와 같은 사이토카인에 의해 유전자 발현이 조절된다. 본 연구에서는 Stat 6 유전자 제거 생쥐와 정상 (wild type, W/T) 생쥐에 carrageenan으로 염증을 유도한 후 족삼리에 침치료를 시행하여 시상하부에서의 유전자 발현 양상을 분석하고자 하였다. 방 법 : BALB/c (W/T, n=12) and BALB/c-Stat 6 유전자 제거 생쥐 (n=12)의 발뒤꿈치 표피에 1% carrageenan을 30 ul 주사하여 염증을 유도하였다. 침은 염증 유도 30분 후에 족삼리(ST36)에 시침하였으며, 염증유도에 의한 부종 증가율을 매 시간마다 측정하여 총 5시간동안 측정하였다. 마이크로에러이는 Stat 6 유전자 제거 생쥐를 염증 유발 군과 염증유발 후 침을 처치한 군으로 나누고, 시상하부를 적출하여 RNA를 분리한뒤 마이크로어레이 프로파일을 분석하였다. 결 과 : 염증에 의한 부종증가율을 비교한 결과, Stat 6 유전자 제거 생쥐 그룹의 부종증가율이 W/T 생쥐의 부종 증가율보다 약 50 % 정도 감소하였으며, 각 3, 4, 5시간째에 유의한 차이를 나타내었다. (각 p<0.05). W.T생쥐군과 Stat 6 유전자 제거 생쥐군 모두에서, 침 처치군이 염증 유발 군에 비해, 염증 유발 2시간 후부터 유의한 감소를 나타내었다. 시상하부의 유전자 발현을 관찰한 결과, 39개의 유전자가 3배 이상 감소하였으며, 19개의 유전자는 3배 이상 증가하였다. 결 론 : W/T 생쥐군과 Stat 6 유전자 제거 생쥐 모두에서 침의 진통효과는 나타나며, 이의 기전에는 시상하부에서의 침 치료에 의한 염증관련 유전자들의 감소와, 항염증과 관련된 유전자들이 증가가 관여하는 것으로 보인다., 10, 11), 내측전완피신경(TE5, 6, 7, 8, 9, 10, 11), 후상완피신경(TE12, 13), 상외측상완피신경(TE13), 외측쇄골상신경(TE14, 15),대이개신경(TE16, 17, 18, 19), 소후두신경(TE19, 20), 이개측두신경(TE20, 21, 22), 안면신경측두지(TE22, 23), 관골측두신경(TE23), 중층에 견갑상신경(TE15), 견갑배신경(TE15), 경상설골근신경(TE17), 후이개신경(TE18, 19, 20), 안면신경측두지(TE20, 21, 22), 심층에 후골간신경(TE5, 6, 7), 요골신경심지(TE8, 9, 12, 13), 견갑상신경(TE14), 액와신경가지(TE14), 부신경(TE16), 안면신경과 부신경가지(TE17), 설인신경(TE17), 설하신경(TE17), 경신경고리(TE17), 미주신경(TE17), 안면신경 (TE18). 3) 혈(血) 관(管) : 천층에 척측정맥배측지(TE1, 2), 고유수장지동맥배측지(TE1), 배측중수골동맥배측지(TE2), 배측중수골정맥(TE3), 척측피정맥(TE4, 5, 6, 7, 8, 9, 10, 11), 배측정맥궁(TE4), 부요측피정맥(TE6, 8, 9),요측피정맥(TE10, 11), 후견봉정맥가지(TE13, 14), 후이개동 ${\cdot}$ 정맥(TE16, 17, 18, 19, 20), 전이개동 ${\cdot}$ 정맥(TE20), 천측두동 ${\cdot}$ 정맥(TE22, 23), 중층에 후상완회선동맥(TE14), 견갑배동맥(TE15), 견갑상동맥(TE15),천측두동 ${\cdot}$ 정맥(TE21), 관골측두동 ${\cdot}$ 정맥(TE23), 심층에 배측중수골동맥(TE3), 배측수근동맥궁(TE4), 후골간동맥(TE4, 5, 6, 7, 8, 9), 전골간동맥(TE6, 7, 9)

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Development of Plant BIM Library according to Object Geometry and Attribute Information Guidelines (객체 형상 및 속성정보 지침에 따른 수목 BIM 라이브러리 개발)

  • Kim, Bok-Young
    • Journal of the Korean Institute of Landscape Architecture
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    • v.52 no.2
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    • pp.51-63
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    • 2024
  • While the government policy to fully adopt BIM in the construction sector is being implemented, the construction and utilization of landscape BIM models are facing challenges due to problems such as limitations in BIM authoring tools, difficulties in modeling natural materials, and a shortage in BIM content including libraries. In particular, plants, fundamental design elements in the field of landscape architecture, must be included in BIM models, yet they are often omitted during the modeling process, or necessary information is not included, which further compromises the quality of the BIM data. This study aimed to contribute to the construction and utilization of landscape BIM models by developing a plant library that complies with BIM standards and is applicable to the landscape industry. The plant library of trees and shrubs was developed in Revit by modeling 3D shapes and collecting attribute items. The geometric information is simplified to express the unique characteristics of each plant species at LOD200, LOD300, and LOD350 levels. The attribute information includes properties on plant species identification, such as species name, specifications, and quantity estimation, as well as ecological attributes and environmental performance information, totaling 24 items. The names of the files were given so that the hierarchy of an object in the landscape field could be revealed and the object name could classify the plant itself. Its usability was examined by building a landscape BIM model of an apartment complex. The result showed that the plant library facilitated the construction process of the landscape BIM model. It was also confirmed that the library was properly operated in the basic utilization of the BIM model, such as 2D documentation, quantity takeoff, and design review. However, the library lacked ground cover, and had limitations in those variables such as the environmental performance of plants because various databases for some materials have not yet been established. Further efforts are needed to develop BIM modeling tools, techniques, and various databases for natural materials. Moreover, entities and systems responsible for creating, managing, distributing, and disseminating BIM libraries must be established.