• Agrochemical news magazine
• /
• v.22 no.9 s.172
• /
• pp.45-47
• /
• 2001

• Monthly Duck's Village
• /
• s.209
• /
• pp.76-79
• /
• 2020

• Applied Biological Chemistry
• /
• v.44 no.3
• /
• pp.162-166
• /
• 2001

Effects of light, methyl jasmonate(MJ), 6-benzylaminopurine(BA), thidiazouron(TDZ), and 2,4-dichlorophenoxyacetic acid(2,4-D) on the contents of ecdysteroids (${\beta}$-ecdysone+polypodine B) in Polypodium vulgare L. and Achyranthes japonica Nakai were studied. When the plants of P. vulgare were cultured under light control, the ecdysteroids contents in both leaves and rhizomes decreased with increasing light intensity. The ecdysteroids contents in A. japonica were also lower when cultured under light than under dark. Among the tested plant growth regulators, MJ, BA, and TDZ increased the ecdysteroids contents in both P. vulgare and A. japonica.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.10a
• /
• pp.71-71
• /
• 2018

본 연구는 각시톱시네고사리(Dryopteris hangchowensis Ching)의 전엽체 증식 및 포자체 형성을 위한 번식방법을 구명하고자 수행되었다. 각시톱지네고사리의 식물체를 무가온 온실에 재배하면서 포자를 채취하였으며, 수집된 포자는 기내에서 발아시켜 전엽체를 획득한 다음 계대배양하면서 실험의 재료로 사용하였다. 전엽체 대량증식을 위하여 1/4, 1/2, 1배로 조절한 MS배지와 Knop배지를 조성하였다. 이후 전엽체 300mg을 균일하게 다져 조성된 배지에 8주간 배양하였다. 연구의 결과, 전엽체의 생체중은 1/2MS와 1/4MS에서 3.4g으로 가장 많이 증가하였다. 현미경 관찰의 결과, 두 배지 모두 정상적인 전엽체의 형태로 발달하였으며, 생육도 양호하였다. 포자체 형성을 위한 적정 배양토의 혼합조건을 확인하고자 원예상토, 피트모스, 펄라이트 및 마사토의 혼합비율을 5종류로 달리하여 조성하였다. 준비된 배양토를 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진 후 기내 배양한 전엽체 1g과 증류수를 함께 10초간 분쇄하여 토양표면에 균일하게 분주한 다음 12주간 재배하였다. 연구의 결과, 원예상토, 피트모스, 펄라이트를 1:1:1(v:v:v) 비율로 혼합한 토양에서 포트 당 223.7개로 가장 많은 포자체가 형성되었다. 또한 형성된 포자체의 생육을 조사한 결과, 상기 토양에서 생체중, 엽수, 엽장, 엽폭, 근수, 근장 및 SPAD value 등의 생육수치가 우수하였다. 따라서 각시톱시네고사리의 전엽체 증식에 적합한 배지는 경제성과 생육수준을 고려하여 1/4MS 배지가 적합하며, 포자체 대량생산을 위해서는 원예상토, 피트모스, 펄라이트를 1:1:1(v:v:v)로 혼합한 토양이 적합하다고 판단되었다.

• Korean Journal of Plant Resources
• /
• v.19 no.2
• /
• pp.348-354
• /
• 2006

Homogenized prothallus of 6 species (Cyrtomium falcatum, Cyrtomium caryoptideum var. coreanum, Dryopteris varia, Asplenium incisum, Camptosous sibiricus and Phyllitis scolopendrium) were treated with gamma radiation or by chemical mutagenesis with EMS, NMU, $NaN_3$ and colchicine to assess their sensitivities for each treatments and also with the aim of inducing mutations. Generally, decrease of proliferation ratio was dose-dependent and time-dependent. Based on proliferation ratio, optimum dose of gamma irradiation was $5{\sim}10krad$ except in D. varia with 20krad. Optimum condition of EMS treatment was considered as 50mM for 3h and for NMU as $5{\sim}10mM\;for\;1{\sim}3h$. Optimum condition of $NaN_3$ treatment was considered as $0.5{\sim}1mM\;for\;1{\sim}3h$. For colchicine, there were significant differences between species as to the proliferation ratios of prothallus for each treatment.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.5 no.2
• /
• pp.34-38
• /
• 2002

This studies were performed to determine the effects of indoor relative humidity conditions on the growth of Arachniodes aristata and Pyrrosia lingua in native fern and investigate an applicable possibility of indoor plants. The response of Arachniodes aristata and Pyrrosia lingua was evaluated in growth chamber (KG-8407-87) to 3 relative humidity and 3 soils. Relative humidity was applied to 25%, 50%, 90%; soils were prepared based on soil mixture(SM), top field soil(TF), masa soil(MS). Results of experiments are as follows ; 1. Under a low humidity condition, such as 25%, Water contents, chlorophyll content, ornamental value of Arachniodes aristata and Pyrrosia lingua decreased much more a 50-90%. 2. The growth of Arachniodes aristata were best in pots under a soil mixture(SM) but Pyrrosia lingua were field top soil(FT) under 25%. It seemed that Pyrrosia lingua due to tolerance of desiccation more than Arachniodes aristata. Though these studies, coefficient of relative humidity and soil was plays an important role in Arachniodes aristata and Pyrrosia lingua growth in indoor environments.

• Korean Journal of Plant Resources
• /
• v.23 no.4
• /
• pp.350-359
• /
• 2010

The distribution of the Mankyua chejuense was restricted to the northeast region of Jeju Island. It grows in the isolated pit crater-like swamp areas of various extent and shape, where the height is lower compared to the adjacent areas, and the adjacent regions contained a evergreen broad-leaved forests, a shrubbery, and a grassland vegetation. It contained 147 taxa of 61 families, 112 genera and 147 species in the habitats. The life form of the flora showed that the habitat of M. chejuense is different from the surrounding others, in particular, the ratio of the Therophytes and the Hydrophytes appeared highly. Preservation of habitat conditions is very important for stable maintenance the flora, and the surrounding area including habitats has to be designated a protection area for habitat preservation.

• Journal of Plant Biology
• /
• v.38 no.1
• /
• pp.115-119
• /
• 1995

In order to clarify the relationship between the activity and age of vacular cambium in the rhizome of Botrychium ternatum, the cambia of the 6th and 10th internodes from its shoot apex were compared ultrastructurally and histochemically. The cambium in the 6th internode had two to four radially arranged cells in each row, and the cells are radially flattened and had thiner tangential walls than radial. The cambial cells were ultrastructurally characterlized by the presence of a large vacuole, few storage materials such as starch grains and lipid droplets, and various cell organelles such as mitochondria, endoplasmic reticulums and dictyosomes in cytoplasm. On the other hand, the cells of cambium in the 10th internode showed irregular arangement and somewhat enlargement in radial plane. The cells were filled with numerous storage materials and small vacuoles and had few cell organelles except for mitochondria. In addition, the activity of acid phosphatase appeared in the cambium of the 6th internode but of the 10th. From the above results, it is clear that the vascular cambium of the 6th internode was active and that of the 10th was dormant. Thus we reached a conclusion that the rhizome of '||'&'||'trychium ternatum has determinate cambium.

• Korean Journal of Plant Resources
• /
• v.24 no.4
• /
• pp.395-403
• /
• 2011

This study was conducted to investigate the habitat positional environment and vegetation properties of Mankyua chejuense. The habitats were classified into two types depending on the depth of the habitat, the ratio of rock exposure, the dominant species and the surrounding vegetation, which affected the vegetation of the habitats. The habitats with a high ratio of rock exposure showed a distinctive geographical boundary to the adjacent region and most of them were composed of trees that grow in humid environment. On the contrary, in the soil-rich habitats, the depth was shallow, soil layer was well developed, and the trees were introduced from the adjacent areas. However, the dominant species in the herbaceous layer were aquatic plants, which indicated that the habitats had the properties of wetland. Therefore, it was found that thes habitats of Mankyua chejuense have the properties similar to those of marshland. For the preservation of Mankyua chejuense, it is very important to make the habitats maintain such properties of wetland.

• Korean Journal of Plant Resources
• /
• v.24 no.4
• /
• pp.351-357
• /
• 2011

This study was carried out to investigate the effective extraction condition for increase of phytoecdysteroids from fronds of Matteuccia struthiopteris (FMS) and Osmunda japonica (FOJ). Lyophilizated fronds were mixed with three different solvents (MeOH, 80% EtOH or water) and then extraction was carried out by using six different methods, such as, immersion (room temp.), heating (60$^{\circ}C$), stirring (200 rpm) for 6 h, or sonication in 42 kHz ultrasonic bath for 15, 30 and 45 minutes. Contents of 20-hydroxyecdysone (20E) and ponasterone A (PonA) were measured by using HPLC after purification of the extracts by $C_{18}$ cartridge. Altogether, our results indicate that the extraction using sonication with MeOH as a solvent (for 30 minutes) was the most effective condition for 20E and PonA from both MFS and FOJ. Resulting contents of 20E from FMS and FOJ were 66.76 and 104.48 ${\mu}g{\cdot}g^{-1}$ and PonA were 53.43 and 43.82 ${\mu}g{\cdot}g^{-1}$, respectively.