• Title/Summary/Keyword: 고농도 질소

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Mechanism of Decrease in Lung Injury by Low Dose of Endotoxin During Hyperoxia in the Rats (저용량의 내독소가 쥐에서 고농도의 산소에 의한 급성폐손상을 경감시키는 기전)

  • Song, Jeong-Sup;Yoon, Hyung-Kyu;Kim, Young-Kyoon;Kim, Kwan-Hyung;Moon, Hwa-Sik;Park, Sung-Hak
    • Tuberculosis and Respiratory Diseases
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    • v.53 no.2
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    • pp.148-160
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    • 2002
  • 배 경 : 쥐를 고농도의 산소에 60시간 이상 노출시켰을 때 급성 폐손상이 유발되지만 내독소를 저용량으로 투여시에는 이러한 폐손상이 경감된다고 알려져 있으나 그 기전에 대하여는 확실히 밝혀지지 않고 있다. 산화질소(nitric oxide, NO)는 내독소나 염증성 사이토카인(cytokine) 등의 자극에 의해서 폐내 여러 염증세포에서 만들어지며 이 산화질소는 경우에 따라 우리 몸에 이롭거나 해로운 양면성을 지니고 있다. 저자들은 쥐에서 고농도의 산소에 의한 폐손상이 저농도의 내독소 투여로 경감되는 기전에, 산화질소가 중요한 역할을 하는지 또는 황산화효소나 다른 항염증성 사이토카인이 중요한 역할을 하는지를 규명하고자 하였다. 방 법 : 총 120마리의 쥐 (Sprague-Dawley rat)를 24마리씩 5군으로 나누어 대조군은 실내 공기를, 고농도 산소군은 100%의 산소를 100%의 산소를 60시간 투여하였고 내독소군은 100% 산소 투여시 2일간 저용량의 내독소를 투여하였다. 다른 두 군은 산화질소 합성 억제물인 aminoguanidine(AG)과 N-nitro-L-arginine methyl ester (L-NAME)를 각각 2일간 고농도 산소와 내독소에 더하여 투여하였다. 각각의 군에서 폐손상의 정도와 사망률을 관찰하고 superoxide dismutase(SOD), catalase, nitric oxide, IL-6, IL-11을 기관지폐포세척액에서 측정하고, 고농도산소 투여군의 폐조직에서 iNOS synthase rnRNA의 발현을 비교하였다. 결 과: 1. 100%의 산소에 60시간 노출시켰을 때 쥐의 사망률은 8.3% 이었고 내독소 투여군은 4.2%, NAME 투여군이 37.5%, AG 투여군이 25%로 산화질소 합성 억제제에 의하여 사망률의 증가가 관찰되었다. 2. 폐의 손상 정도를 나타내는 폐의 wet/dry 중량비와 늑막액도 100%의 산소에 노출된 군에서 증가되었고 내독소 투여에 의하여 감소되었으며 NAME나 AG 투여군에서는 오히려 증가되었다. 3. 이러한 내독소에 의한 폐손상 억제효과가 항산화효소인 SOD나 catalase, 또는 protective cytokine인 IL-6나 IL-11등의 증가와 관련이 있는지를 관찰하였으나 이들 모두에서 유의한 변화를 관찰하지 못하였다. 4. 산화질소는 100% 산소에 노출시킨 군에서도 증가하였으나 내독소 투여군에서 유의하게 더욱 증가하였고 이는 L-NAME 나 aminoguanidine의 투여시 감소하였다. 5. iNOS mRNA의 발현도 내독소 투여군에서 유의하게 증가하였다. 결 론 : 쥐의 고농소 산소 투여에 의한 폐손상은 저용량의 내독소 투여로 경감되며, 이는 주로 내독소 투여에 의한 iNOS mRNA의 발현을 유도하여 생성된 산화질소의 증가에 기인하는 것으로 생각된다.

Behaviors of Pollutants and Microorganisms in an Anaerobic Digestion of Propionate Containing High Ammonia Nitrogen Level (고농도 암모니아성 질소를 함유한 프로피온산의 혐기성 분해시 오염물질 및 미생물 거동)

  • Lee, Chae-Young;Kim, Dae-Sung;Ahn, Won-Sik;Shin, Hang-Sik
    • Journal of the Korea Organic Resources Recycling Association
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    • v.14 no.3
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    • pp.126-137
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    • 2006
  • Behaviors of simple organic compound and granular sludge in an upflow anaerobic sludge blanket (UASB) reactor treating propionate at high ammonia nitrogen levels were investigated for 12 months. The UASB reactor achieved about 80% removal of chemical oxygen demand (COD) at ammonia nitrogen concentration up to 6000 mg-N/L. At higher concentration of ammonia nitrogen, the propionate in the effluent increased whereas the acetate was very low. At ammonia nitrogen concentration of 8000 mg-N/L, the volatile suspended solids (VSS) increased sharply due probably to the decrease of the content of extracellular polymer (ECP) although methane production was very low. The specific methanogenic activity (SMA) using formate, acetate, and propionate as substrate to granules decreased as ammonia nitrogen concentration increased. The ammonia nitrogen concentration $I^{50}$, causing 50% inhibition of SMA were 2666, 4778 and 5572 mg-N/L, respectively. The kinetic coefficients of ammonia inhibition using formate, acetate, and propionate as substrate were 3.279, 0.999 and 0.609, respectively. The SMA using formate was severely affected by ammonia nitrogen than those using acetate and propionate. This result indicated that the hydrogenotrophic methanogens was most affected by ammonia nitrogen. Granules were mainly composed of microcolonies of methanothrix-like bacteria resembling bamboo-shape, and several other microcolonies including propionate degrader with juxtapositioned syntrophic associations between the hydrogen-producing acetogens and hydrogen-consuming methanogens.

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Technology Development of Return Flow Treatment from Sludge Processing (슬러지 공정 반류수 처리 기술 개발)

  • 김선구;장윤석;박명균;박미경;허용록;최의소;윤주환;길경익
    • Environmental engineer
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    • s.175
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    • pp.56-62
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    • 2001
  • 하수처리장의 농축-소화-탈수 등 전형적인 슬러지처리 공정에서 발생하는 소화조, 농축조 상징액 및 탈리여액 등의 반류수는 유량은 적으나 고농도로써 처리장의 전체 처리효율에 심각한 영향을 주는 것으로 알려져 있는데 외국에서는 이미 반류수의 농도를 감소시키며, 또한 발생된 반류수를 효과적으로 처리하기 위한 기술개발에 대한 광범위한 연구가 활발히 진행되고 있다. 이는 반류수 내 고농도의 질소를 제거하면 기존 처리장에서도 상당한 수준의질소 제거가 가능하다는 판단

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Effects of Nitrogen and Phosphorus Fertilization on Ectomycorrhiza Development, N-Fixation and Growth of Red Alder Seedlings (질소(窒素)와 인산(燐酸) 시비(施肥)가 루브라 오리나무(Alnus rubra Bong.) 묘목(苗木)의 외생균근발달(外生菌根發達)과 질소고정(窒素固定) 및 생장(生長)에 미치는 영향(影響))

  • Koo, Chang-Duck;Molina, Randolph J.;Miller, Steven L.;Li, Ching Y.
    • Journal of Korean Society of Forest Science
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    • v.85 no.1
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    • pp.96-106
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    • 1996
  • Red alder(Alnus rubra Bong.) seedlings inoculated with Frankia only or both Frankia and spores of Alpova diplophloeus(Zeller & Dodge) Trappe & Smith were grown in a greenhouse for ten weeks. The ten-week-old seedlings were fertilized with six nitrogen(N) and phosphorus(P) fertility regimes (no fertilization, 10mM $NH_4NO_3$, 50mM $NH_4NO_3$, 5mM $KH_2PO_4$, 10mM $NH_4NO_3+5mM$ $KH_2PO_4$, and 50mM $NH_4NO_3+5mM$ $KH_2PO_4$) three times a week for ten weeks. The higher N-fertilization significantly increased mycorrhiza formation by greenhouse contaminant mycorrhizal fungi, but decreased N-fixation and P concentration in nodule tissues. P-fertilization significantly increased nodule and shoot dry weight, and P concentration in plant tissues. When N was highly fertilized, however, the P-fertilization effect disappeared in nodule P concentration but doubled in leaf P concentration. A. diplophloeus inoculation significantly increased diameter growth and $CO_2$ exchange rate, but decreased leaf dry weight. Our results suggest that the higher N- or P-fertilization affect nitrogenase activity and mycorrhizal development but the effects are changed by their interactions.

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Process Development of Algae Culture for Livestock Wastewater Treatment Using Fiber-Optic Photobioreactor (축산폐수 처리를 위한 광섬유 생물반응기를 이용한 조류 배양 공정 개발)

  • 최정우;김영기;류재홍;이우창;이원홍;한징택
    • KSBB Journal
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    • v.15 no.1
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    • pp.14-21
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    • 2000
  • In this study, algae cultivation using the photobioreactor has been applied to remove the nitrogen and phosphorus compounds in the wastewater of the livestock industry. The optimal ratio of nitrate and ortho-phosphate concentration was found for the enhancement of removal efficiency. To achieve the high density culture of algae, the photobioreactor consisted of optical fibers wes developed to get the sufficient light intensity. The light could be illuminated uniformly from light source to the entire reactor by the optical fibers. The structured kinetic model was proposed to describe the growth rate, consumption rate of nitrates and ortho-phosphates in algae culture. The self-organizing fuzzy logic controller incorporated with genetic algorithm was constructed to control the semi-continuous wastewater treatment system. The proposed fuzzy logic controller was applied to maintain the nitrated concentration at the given set-point with the control of wastewater feeding rate. The experimental results showed that the self-organizing fuzzy logic controller could keep the nitrate concentration and enhance algae growth.

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A Study on the Removal of Nitrate Nitrogens by Redox Reaction of Zinc Ball (아연볼의 산화·환원 반응을 통한 연속식 질산성질소 처리에 관한 연구)

  • Kim, Joon Hwan;Kim, Jong Hwa;Song, Ju Yeong
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.3
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    • pp.487-494
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    • 2017
  • Since nitrate nitrogen is quite stable in aqueous solution, considerable skill is required to remove it. Low concentrations of nitrate nitrogen are easily removed, while high concentrations of nitrate nitrogen are difficult to remove. This study is to show that nitrate nitrogen in the form of gaseous nitrogen can be removed by using zinc ball with a diameter of about 3mm and to test the removal characteristics of nitrate nitrogen under various reaction conditions. As a result of this study, the treatment efficiency of nitrate nitrogen by continuous treatment with zinc ball was about 80%. However, there is a problem that the wastewater must be maintained in an acidic atmosphere of about pH 2, and the treated wastewater must be neutralized and discharged.

Electrochemical Reduction of Nitrate Ion in an Aqueous Solution (수용액에서 질산성 질소의 전기화학적 환원거동)

  • Park, J.K.;Jeon, C.J.;Lee, C.K.
    • Proceedings of the Korean Institute of Resources Recycling Conference
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    • 2003.10a
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    • pp.246-251
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    • 2003
  • 전기분해법을 이용하여 수용액 중의 질산성 질소의 환원거동에 대한 연구를 통하여 수용액중의 질산 함량을 제어하는 연구를 수행하였다. 촉매전극을 채택한 복극전해조에서 30분의 조업에 질산 100ppm 이하의 저농도 용액은 70%, 300ppm 이상의 고농도의 경우는 90%까지 질소를 용이하게 제거할 수 있었다. 초기 질소농도가 증가하면서 한계전류밀도도 크게 증가하였으며, pH가 감소할수록 환원전류가 증가하였다. 그리고 수용액의 pH는 질소 환원반응기구에 큰 영향을 주는 것으로 판명되었으며, 산성에서는 질소형태로 중성 혹은 염기성에서는 암모니아 형태로 환원되는 것으로 추정된다.

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Effects of Operating Parameters on the Removal Performance of Nitrate-nitrogen by Electrodialysis (전기투석을 이용한 질산성 질소의 제거 시 운전인자의 영향)

  • Lee, Gwan-Ho;Lee, Gang-Choon
    • Clean Technology
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    • v.15 no.4
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    • pp.280-286
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    • 2009
  • To evaluate the feasibility of electrodialysis for nitrate-nitrogen removal from wastewater, the effect of operating parameters on the removal of nitrate-nitrogen was experimentally estimated. The limiting current density (LCD) linearly increased with the nitrate concentration and the flow rate. The time when the nitrate concentration of diluate reached at 20 mg/L was linearly proportional to concentration of diluate, and the concentration of concentrate did not affect the removal rate. Increase in the flow rate gave a positive effect on the removal rate and became insignificant at How rates greater than 1.6 L/min. The removal rate increased with the applied voltage, but the increment in the removal rate decreased as the applied voltage approached the LCD. From the operation of the electrodialysis module used in this research, the flow rate of 1.6 L/min and the voltage corresponding to the 80~90% of LCD were found be the optimum operating condition for the nitrate removal from highly concentrated nitrate-nitrogen solutions.

Cencept of adaptability for heavy nitrogen in view of nitrogen metabolism -II. Nitrogen metabolism under the change of itrogen nenvironment (내비성(耐肥性)에 관(關)한 질소대사적(窒素代謝的) 개념(槪念) -II. 질소환경(窒素環境) 변화(變化)에 따른 질소대사(窒素代謝))

  • Yoon, Jong Hyuk;Park, Hoon;Cho, Sung Jin
    • Korean Journal of Soil Science and Fertilizer
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    • v.7 no.1
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    • pp.49-53
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    • 1974
  • Varietal difference in nitrogen metabolism was investigated under water culture system with high (50 or 80ppm) and low (10 or 40 ppm) levels of nitrogen and with two-week minus nitrogen treatment at maximum tillering and heading stage using a leading local variety, Jinheung and high-yielding IR667 line (newly bred tropical variety). 1. In high nitrogen level Jinheung showed higher yield than IR667. vise versa in low nitrogen level. 2. Poor yield of IR667 at high nitrogen may be due to ammonium toxicity that was eliminated by minus nitrogen from culture solution. 3. IR667 was more sensitive to the change of nitrogen environment. 4. With high nitrogen medium Jinheung showed nigher nitrogen uptake and higher capacity of protein synthesis than IR667, and vise versa at low nitrogen medium. 5. From the above facts it could be concluded that Jinheung has higher metabolic adaptability for heavy nitrogen while IR667 has higher structural adaptability for heavy mitrogen and that better productive adaptability will be resulted in the combination of both characteristics.

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Temporal Variation of Water Quality of the Western Chinhae Bay in Summer (진해만 서부해역의 하계 수질의 시간변동 특성)

  • Cho Hyeon-Seo;Lee Dae-In;Yoon Yang-Ho;Lee Moon-Ok;Kim Dong-Myung
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.7 no.1
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    • pp.13-21
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    • 2004
  • Temporal changes of Chl-α, physical and chemical factors were investigated by diurnal observation at 2-hour interval at three fixed stations in the western Chinhae Bay from 12 Aug. to 13 Aug. 1999. Difference of dissolved oxygen between surface and bottom layer was maximum when the thermocline were strong. Organic distribution such as COD was affected by the growth of phytoplankton. Limitting factor was nitrogen, that is, inorganic nitrogen plays a significant role on regulating the algal growth. Surface distribution of dissolved inorganic nitrogen was very low compared to bottom layer by uptake of organisms. Maximum value of Chl-α at station C2 and C11 were observed from subsurface layer, ranges of which exceeded possibility concentration of red tide outbreak, 10 mg/㎥. On the other hand, that of C15 exist at surface layer. In this area, DIN and DIP concentrations increased by input sources such as rainfall and benthic flux before the bloom of phytoplankton. Accumulation of phytoplankton occurred at subsurface layer by the rapid uptake of DIN, especially nitrate ion, when strong thermocline existed as approach to the afternoon, which led to the increase of organics in water column and oxygen deficiency water mass at bottom layer until late at evening. Since then, DIN increases gradually as water temperature decrease to minimum. The quantitative understanding of nitrogen of fluxed to and from the various sources is necessary for environmental management.

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