• Horticultural Science & Technology
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• v.30 no.3
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• pp.286-293
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• 2012

The resistance to Tobamovirus in Capsicum spp. has been known to be controlled by five different alleles ($L^0$, $L^1$, $L^2$, $L^3$, and $L^4$) of L locus on the telomere of long arm of pepper chromosome 11. To develop a set of molecular markers differentiating all the alleles of L locus, we used five pepper differential hosts including Capsicum annuum Early California Wonder (ECW, $L^0L^0$), C. annuum Tisana ($L^1L^1$), C. annuum Criollo de Morelos 334 (CM334, $L^2L^2$), Capsicum chinense PI 159236 ($L^3L^3$), and Capsicum chacoense PI 260429 ($L^4L^4$). Developing a series of CAPS or SCAR markers specifically linked to the alleles was allowed by the sequence comparison of PCR amplicons of the $L^3$-linked markers (189D23M, A339, and 253A1R) and BAC sequences (FJ597539 and FJ597541) in the pepper differentials. Genotypes deduced by these markers in 48 out of 53 $F_1$ hybrids of commercial pepper varieties were consistent with their phenotypes by bioassay using Tobamovirus pathotypes ($P_0$, $P_1$, and $P_{1,2$). Consequently, these markers can be useful to differentiate L alleles and for breeding Tobamovirus resistance in pepper with marker-assisted selection.

• Journal of Plant Biotechnology
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• v.45 no.1
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• pp.63-70
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• 2018

Brazzein is the smallest sweet protein and was isolated from the fruit pulp of Pentadiplandra brazzeana Baillon, native to tropical Africa. From ancient times, the indigenous people used this fruit in their diet to add sweetness to their daily food. Brazzein is 500 to 2000 times sweeter than sucrose on a weight basis and 9500 times sweeter on a molar basis. This unique property has led to increasing interest in this protein. However, it is expensive and difficult to produce brazzein other than in its native growing conditions which limits its availability for use as a food additive. In this study, we report high production yields of, brazzein protein in transgenic rice plants. An ORF region encoding brazzein and driven by the $2{\times}CaMV\;35S$ promoter was introduced into rice genome (Oryza sativa Japonica) via Agrobacterium-mediated transformation. After transformation, 17 regenerated plant lines were obtained and these transgene-containing plants were confirmed by PCR analysis. In addition, the selected plant lines were analyzed by Taqman PCR and results showed that 9 T0 lines were found to have a single copy out of 17 transgenic plants. Moreover, high and genetically stable expression of brazzein was confirmed by western blot analysis. These results demonstrate that recombinant brazzein was efficiently expressed in transgenic rice plants, and that we have developed a new rice variety with a natural sweetener.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.1
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• pp.93-100
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• 1985

Five winter and five spring wheat cultivars of diverse genetic backgrounds were evaluated to examine different developmental responses in terms of stages of the life cycle and grain yield when grown under the different planting dates. Greatest difference in growth and developmental patterns of the winter and spring wheat cultivars occurred in stem elongation, booting, inflorescence emergence and anthesis. The growth stage of stem elongation was found to exhibit larger difference both among planting dates and cultivars. Winter wheat cultivars responded more than spring wheat cultivars to the different planting dates. Winter wheat 'Cho Kwang' and spring wheat 'Jugoku 81' were earlier and exhibited faster growth and development, while winter wheats 'Yamhill' and 'Hyslop' were later in growth and development, but exhibited faster grain filling and higher rate of grain filling, resulting in higher grain yields. Crosses between winter and spring wheat gene pools would result in earlier maturity and higher productivity for both winter and spring wheat cultivars. For developing early maturing wheat cultivars for multiple cropping sequences while maintaining productivity, selection for earliness trait should be started at the stem elongation stage. Furthermore, the breeding materials should be planted at several times for selection of shorter life cycle genotypes adaptable to the cropping sequences. This is due to the genotype x planting date interactions.

• Journal of Life Science
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• v.20 no.1
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• pp.22-26
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• 2010

Seven rice germplasms with red pigmentation within the pericarp were isolated from a large mutant collection. These red pericarp phenotypes resulted from the functions of the Rc, Rd and RdRc genes. Among them, two brown pericarp of the Rc type, four red pericarp of the RdRc type, and one white pericarp of the Rd type were identified. Morphological and agronomic characteristics of those rice germplasms were studied. The Rc type germplasms have the faint red or brown color pericarp and the Rd types produce the white pericarp, whereas the RdRc type germplasms have the dark red pericarp. Most of the important agronomic characteristics including plant stature, tillering ability, spikelet fertility, and total grain yield were lower in the colored rice than those of the wild-type control. All of the studied colored rice germplasms had a tendency of easy seed-shattering in comparison to the control. These characteristics of newly identified germplasms will be useful for identifying the genes responsible for pericarp color phenotype determination.

• Korean Journal of Agricultural Science
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• v.1 no.1
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• pp.5-8
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• 1974

To obtain several foundamental information for the breeding of Korean Ginseng. 16 line selecteds in Korea were investigated and analyzed. The results obtained are as follows : 1. In all quantitative genetic characters of Korean Ginseng, the stem length showed the highest heritability(0.7050) and the leaf length the lowest(0.5674). 2. The genotypic correlation was showen the highest value (0.8649) at between the leaf length and the stem diameter and the lowest value(0.8649) at between the leaf length and the stem length, but the phnotypic correlation was shown the highest value (0.8788) at between the stem length and the stem dimeter and the lowest value (0.7950) at between the leaf length and stem length. 3. The co-heritability between the stem length and the stem diameter showed the highest value(0.6124) and that between the leaf length and the stem length showed the lowest value(0.5471). 4. The inter-class correlation among the planting location showed the highest value at the stem length and the lowest value at the stem diameter.

• Journal of Plant Biotechnology
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• v.32 no.2
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• pp.85-90
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• 2005

This study was conducted to investigated the major characteristics of genetically modified rice of 'Milyang 204' originated from Dongjinbyeo compared to a non-transgenic rice varieties Dongjinbyeo and Jun-ambyeo. Basta resistant transgenic rice lines carrying bar gene produced by the Yeongnam Agricultural Research Institute were evaluated for their agronomic characters. The transgenic Japonica rice of 'Milyang 204' showed inferior phenotypic traits compared to a non-transgenic rice variety Dongjinbyeo and Junambyeo. On the basis of UPOV (Union Internationale Pour la Protaection des Obtentions Vegetables) and NSMO(National Seed Management Office) the transgenic 'Milyang 204' showed difference in some traits out of some agronomic traits, such as leaf color, angle of flag leaf, number of spikelets, culm length, white core and white belly compared to the non-transgenic varieties rice.

• Journal of Sericultural and Entomological Science
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• v.44 no.1
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• pp.4-8
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• 2002

We conducted this study to investigate the morphological characteristics and physiological effects of mulberry leaves and fruits with wild varieties. Morphologically sampling materials were showed Morus bombycis type and Morus alba type characteristics. Water contents of mulberry fruits were higher than that of mulberry leaves. Sugar degree of sampling materials was 5.1∼22.7 Brix %. The yield of 85% MeOH extract from freezing dried Mulberry Fruits was 60.2% and Hexane, BuOH and H$_2$O fraction from MeOH extract were 2.8%, 4.6%, 92.3% respectively. In the cholesterol-induced hyperlipidemia rats administered with subfraction of mulberry fruit extract, total cholesterol and serum triglyceride were decreased in the MeOH extract group and H$_2$O soluble fraction group. Also H$_2$O soluble fraction group decreased GPT, GOT and LDH level. Therefore, the above results suggested that mulberry fruit with wild varieties can help to maintain normal liver functions and to protect hyperlipidemia.

• Journal of Life Science
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• v.21 no.12
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• pp.1659-1665
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• 2011

In Citrus, an $F_1$ segregation population of 150 plants was constructed from a cross between 'Kiyomi' (C. unshiu ${\times}$ C. sinensis) carrying the male sterility trait and 'Jinkyool' (C. sunki). Sequence-related amplification polymorphism (SRAP) combined with bulked segregant analysis was used to develop markers linked to male fertility. In the $F_1$ population, 66 out of 150 seedlings had aborted anthers and the ratio of male sterile plants to fertile plants in the progenies matched the expected Mendelian segregation ratio of 1:1 ($x^2$ =2.16 at p=0.05). From the profiling of the 197 SRAP primer sets, three SRAP primer sets (F4/R27, F39/R60, and F15/R37) that were closely linked to the target trait were identified and successfully converted into a sequence characterized amplified region (SCAR) marker for selection of male fertility in citrus. The SCAR marker, using the pMS 33U/pMS 1462L primer set specifically, produced a single 1.4-Kb fragment that was linked to male fertility. Our results suggested that this SCAR marker can be useful for marker-assisted selection of male sterile individuals in breeding $F_1$ progenies in Citrus.

• FLOWER RESEARCH JOURNAL
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• v.18 no.3
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• pp.216-219
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• 2010

An Asiatic lily cultivar 'Yeri' was developed in 2005 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA), Korea. The cross was made in 1993 between Asiatic lily 'Geneve', a light pink colored cultivar, and 'Montreux', deep purple colored cultivar. The first selection was done and was tentatively named as 'A95-68' in 1995. After in vitro multiplication and bulbing production, growth and flowering characteristic tests were conducted from 1996 to 2003. The evaluation of characteristics was performed and named as 'Wongyo C1-19' in 2005 that was registered as 'Yeri' to the registration office of Korea Seed & Variety Service. 'Yeri' flowered at the first of July and grew average 34.6 cm stem in length. Flowers bloomed facing upward, unspotted in petals and deep purple (RHS, RP58A). The size of flower was 13.3 cm. Mean petal length and width was 7.7 cm and 2.7 cm, respectively. Leaves were 5.1 cm long and 0.5 cm wide, respectively. The weight and size of bulb were 9.6 g and 11.7 cm, respectively. Year-round flowering can be done by storing the bulb under $-1.5^{\circ}C$ conditions. For forced cultivation, it was necessary to add calcium to the fertilizer or remove side scales to prevent leaf scorch. It was needed to control Botrytis disease in wet season.

• FLOWER RESEARCH JOURNAL
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• v.19 no.4
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• pp.251-254
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• 2011

A new standard white-pink flower rose cultivar 'Innocence' was bred from the cross between white standard cultivar 'Juvena' and green-white standard cultivar 'Green Success' at the Jeollanamdo Agricultural Research and Extension Services (JARES). The cross was made in 2001 and, 'Innocence' was finally selected in 2006 after investigating characteristics for three times from 2004 to 2006. 'Innocence' a white-pink standard cultivar has good flower shape with few prickles. The major characteristics of this cultivar were $118.7stems{\cdot}m^{-2}$ in year yield, 68.0 cm in length of cut flower, 10.9 cm in flower diameter, 34.4 in petal number, and 10.7 days in vase life. This cultivar can be propagated by both cutting and grafting. The consumer's preference of this cultivar was relatively higher than that of control cultivar, 'Rose Yumi'.