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Study on the Cooling Mechanism in a Cryogenic Cooling System (극저온 냉각 챔버 내 냉각 메커니즘 연구)

  • SEONGWOO LEE;YOUNGSANG NA;YOUNGKYUN KIM;SEUNGMIN JEON;JUNHO LEE;SUNGWOONG CHOI
    • Transactions of the Korean hydrogen and new energy society
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    • v.35 no.2
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    • pp.146-151
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    • 2024
  • The demand for research on materials with excellent cryogenic strength and ductility has been increasing, particularly for applications such as liquid hydrogen (20 K) storage tanks. To effectively utilize liquid hydrogen, a system capable of maintaining and operating at 20 K is essential. Therefore, preliminary research and verification of the cooling system are crucial. In this study, a heat transfer analysis was conducted on a cooling system to meet the cryogenic environment requirements for cryogenic hydrogen chamber, which are conducted at liquid helium temperatures (4 K). The cooling mechanism in a helium cooling system was examined using numerical analysis. The numerical cooling trends were compared with experimentally obtained cooling results. The good agreement between numerical and experimental results suggests that the numerical approach developed in this study is applicable over a wide range of cryogenic systems.

The Late Cretaceous Emplacement Age of Masan Hornblende-Biotite Granite (마산 각섬석-흑운모 화강암의 연령: 후기 백악기 정치연령)

  • Lee, Tae-Ho;Park, Kye-Hun;Kim, Jeongmin;Kim, Myoung Jung
    • The Journal of the Petrological Society of Korea
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    • v.26 no.1
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    • pp.1-11
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    • 2017
  • We have dated the K-Ar, Ar-Ar and U-Pb ages of the Masan hornblende-biotite granite in the southern Cretaceous Gyeongsang basin to constrain its emplacement age. The ~108 Ma hornblende K-Ar age obtained in the study is similar to the previously reported Rb-Sr age. However, the single grain total fusion $^{40}Ar/^{39}Ar$ dating on hornblende failed to yield statistically meaningful ages because the isotopic system was open during its alteration. Thus the hornblende K-Ar age in the study is also unlikely to be reliable. The single grain total fusion $^{40}Ar/^{39}Ar$ dating on biotite yielded an average age of $75.8{\pm}3.0Ma$. Apart from scattered data in the range of ~45-75 Ma, the average age increased to ~80 Ma. The SHRIMP and LA-MC-ICPMS U-Pb isotopic compositions of zircon from the Masan hornblende-biotite granite yielded its emplacement age as $87.6{\pm}2.7Ma$ and $86.8{\pm}0.4Ma$, respectively. It is thus likely that the ~80 Ma $^{40}Ar/^{39}Ar$ age of biotite might reflect the cooling age of Masan hornblende-biotite granite or the thermal influences from later intense igneous activities in the Gyeongsang basin.

Ginsenoside Contents and Antioxidative Activities from Red Ginseng Treated with High Hydrostatic Pressure (초고압 처리한 홍삼의 ginsenoside 함량 및 항산화 활성)

  • Shin, Chang-Sik;Lee, Do-Hyun;Kim, Sung-Han;Shin, Min-Ho;Jeong, Chang-Ho;Shim, Ki-Hwan
    • Journal of agriculture & life science
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    • v.44 no.6
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    • pp.133-140
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    • 2010
  • This study was carried out to investigate the changes of ginsenoside contents and antioxidative activities from red ginseng after treated with high hydrostatic pressure (RGHHP). Crude saponin content in traditional red ginseng (TRG) and RGHHP were 21.93 and 27.29 mg/g, respectively. The contents of total phenolics, crude saponin and ginsenoside increased after treated with high hydrostatic pressure. TRG and RGHHP showed an increase 25.60% the highest content of Rb1 (14.10 mg/g and 17.71 mg/g). Also, Rg3 content compared with TRG and RGHHP increased 10.46%. The radical scavenging activity of hot water extract from red ginseng against the DPPH and ABTS radicals increased with the increasing amount of extract and RGHHP higher than TRG. The reducing power and ferric reducing antioxidant power (FRAP) assays of the red ginseng were increased in a dose dependent manner. The FRAP of TRG and RGHHP were 0.30 and 0.36 absorbance, respectively at a concentration of 10 mg/mL. The present results suggest that RGHHP would have the protective potential from oxidative stress induced by free radicals.

Fishes distribution and their connection to artificial reefs off Bukchon, Jeju Island using geographic information system (지리정보시스템을 활용한 제주도 북촌의 인공어초해역에서 어류 분포와 어초와의 관계)

  • KANG, Myounghee;FAJARYANTI, Rina;JUNG, Bongkyu;YOON, Eun-A;MIN, Eunbi;LEE, Kyounghoon;OH, Woo-Seok;PARK, Geunchang;SHIN, Young-Jae;CHOI, Yong-Suk;YI, Byung-Ho;HWANG, Doojin
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.55 no.2
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    • pp.121-128
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    • 2019
  • Various artificial reefs provide the fish habitat and nursery, and contribute the improvement of fisheries productivity. The evaluation methods of fishery resources in the artificial reefs have been done by fishing, scuba diving, underwater camera, and scientific echo sounder/sonar. There are a number of studies using echosounders on the quantitative and qualitative evaluations of artificial reefs in various seas around the world. This study focused on the spatial distribution of fishes around artificial reefs and the influential area of reefs off Bukchon, Jeju Island. Not only acoustic data but also various properties of artificial reefs were used in the geographic information system to extract relevant results. As a result, the major material of reefs on this study site was concrete and the number of reefs with that material was the most. The volume of reefs consisted of steel only and steel with riprap was considerably large compared to other reefs. The average NASC in the vertical distribution of fishes in artificial reefs was $31.6m^2/nm^2$ in April, and that was $61.3m^2/nm^2$ in June. The distance between the fish school and their nearest reef in June morning had a wide range from 750 to 3250 m. On the basis of the influence ray of artificial reefs, it had a tendancy of NASC to decrease with distance from the reef in the June morning. It is a preliminary study to present the geospatial analysis example to understand a better way of comprehensive artificial reef environments.

Analysis of Physiological Activity and Cytotoxicity of Residue Extracts after Pepper Harvest (고추 수확 후 잔재물 추출물의 생리활성과 세포독성 분석)

  • Kim, Tae-Won;Jeon, Byeong-Gyun;Lee, Sung-Ho
    • Journal of Life Science
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    • v.30 no.12
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    • pp.1085-1091
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    • 2020
  • The purpose of this study was to investigate the value of residue discarded after harvesting a species of pepper (Capsicum annuum L.). This study analyzed the physiological activity and cytotoxicity of a fermented extract and a hot water extract gathered from the residues of the Cheongyang pepper and the Nokkwang pepper. The total nitrogen, phosphoric acid, potassium, calcium, and magnesium contents of the fermented extract of the Nokkwang pepper were approximately twice as high as those of the Cheongyang pepper, while the hot water extracts had similar levels of these components across both peppers. Among the microelements, only boric acid, iron, and silicon components were detected, whereas zinc, manganese, molybdenum, and copper components were not detected in either extract. The total polyphenol and flavonoid contents of the fermented extracts were more than two times higher than those of the hot water extracts for both peppers. The DPPH radical scavenging ability (indicating antioxidant activity) of the fermented extract was higher than that of the hot water extract, while the ABTS radical scavenging ability (indicating antioxidant activity) of the hot water extract was higher than that of the fermented extract. A cytotoxicity test was conducted on the extracts using an MTT assay. Both extracts exhibited weak cytotoxicity at all concentrations for both peppers. These results suggest that the extract of residue discarded after harvesting these peppers could be suitable for functional feed or agricultural materials.

Spatial Pattern and Cluster Analysis of University-Industry Collaboration Competency of Korean Universities (대학 산학협력 역량의 공간적 패턴 및 군집분석)

  • HEO, Sun-Young;JANG, Hoo-Eun;LEE, Jong-Ho
    • Journal of the Korean Association of Geographic Information Studies
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    • v.25 no.2
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    • pp.59-71
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    • 2022
  • This study considered regional differences in the university-industry collaboration of Korean universities and performed cluster analysis to identify the spatial range with high university-industry collaboration connectivity. By university establishment type, it was found that the university-industry collaboration capacity of the major national university was superior overall, especially in the technology transfer & commercialization sector and the infrastructure sector, compared to private universities and general national universities. The spatial pattern of university-industry collaboration capacity showed relatively clear differences by city and province. In terms of university-industry collaboration capacity by sector, it was confirmed that the regional gap was not large in the talent training sector and the infrastructure sector, but the regional gap was relatively large in the technology transfer & commercialization sector and the start-up sector. As a result of the cluster analysis to identify a spatial range with high connectivity in terms of similarity and spatial proximity of university-industry collaboration patterns, it is divided into 15 clusters. It is found that most of major national universities are included in one of 15 clusters where all sectors of university-industry collaboration are strong. Therefore, as a policy measure to achieve regional innovative growth through enhancing the effectiveness of university-industry collaboration, we propose the establishment of a hub & spoke network-type collaboration system in which a major national university acts as a hub and nearby local universities play a spoke role.

Estimation of the Marginal Walking Time of Bus Users in Small-Medium Cities (중·소도시 버스이용자의 한계도보시간 추정)

  • Kim, Kyung Whan;Yoo, Hwan Hee;Lee, Sang Ho
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.28 no.4D
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    • pp.451-457
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    • 2008
  • Establishing realistic bus service coverage is needed to build optimum city bus line networks and reasonable bus service coverage areas. The purposes of this study are understanding the characteristics of the present walking time and marginal walking time of small-medium cities and constructing an ANFIS (Adaptive Neuro-Fuzzy Inference System) model to estimate the marginal walking time for certain age and income. The cities of Masan, Chongwon and Jinju are selected for study cities. The 80 percentile of present walking time of bus users of these cities are 10.2-11.1 minutes, thus the values are greater than the 5 minutes of the maximum walking time in USA and the marginal walking times of 21.1-21.8 minutes are much greater. An ANFIS model based on pulled data of the cities are constructed to estimate the marginal walking time of small-medium cities. Analyzing the relationship between marginal walking time and age/income by using the model, the marginal walking time decreases as the age increases, but is near constant from the age of 25 to 35. And the marginal walking time is inversely proportional to the income. In comparing the surveyed and the estimated values, as the statistics of coefficient of determination, MSE and MAE are 0.996, 0.163, 0.333 respectively, it may be judged that the explainability of the model is very high. The technique developed in this study can be applied to other cities.

Cytotoxicity of Particulate Matter in Various Human Cells Lines (미세먼지가 다양한 사람 세포주에 미치는 세포 독성)

  • Lee, Ji-Hyeon;Lee, Joo-Yeong;Kim, Mi-Jeong;Kim, Hyeon-Ji;Lee, Sung-Ho;Jeon, Byeong-Gyun
    • Journal of Life Science
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    • v.29 no.6
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    • pp.724-734
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    • 2019
  • The present study investigated the cytotoxicity of particulate matter (PM) derived from car air filter (outdoor PM) and home cleaner filter (indoor PM) in the various human cell lines. Each outdoor and indoor PM were harvested by ethanol extraction method, subsequently sieved with 10 um filter paper, sterilized with autoclave and added to culture media. The half maximal inhibitory concentration ($IC_{50}$) values was significantly (p<0.05) lower in the outdoor PM, compared with indoor PM, and the significantly (p<0.05) higher $IC_{50}$ values were observed in the cancer cell lines (A-549 lung adenocarcinoma and AGS stomach adenocarcinoma), than those of normal MRC-5 fibroblasts and dental papilla tissue derived-mesenchymal stem cells (DSC). After being exposed to $100{\mu}g/ml$ outdoor PM for 7 days, the population doubling time (PDT) was significantly (p<0.05) increased in especially MRC-5 and DSC cell lines, compared with untreated cell lines. Further, the expression of senescence-associated ${\beta}$-galactosidase activity was up-regulated in all the cells exposed to outdoor PM than those of untreated control. Besides, the expression level of inflammation-associated genes, such as cyclooxygenase-2 (COX-2) and interleukin-6 (IL-6) was found to be significantly (p<0.05) increased in the outdoor PM-treated cell lines than those of untreated cell lines. Our results showed that PM induces the cytotoxicity via arrest of cell growth, cell damage and inflammation response.

Selection of a Soybean Line with Brown Seed Coat, Green Cotyledon, and Tetra-Null Genotype (갈색종피와 녹색자엽 및 Tetra Null 유전자형을 가진 콩 계통 선발)

  • Sarath Ly;Hyeon Su Oh;Se Yeong Kim;Jeong Hwan Lee;Jong Il Chung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.3
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    • pp.114-120
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    • 2023
  • Soybean is the one of the most important crops for providing quality vegetable protein to umans and livestock. Soybean cultivars with a brown seed coat have a wide range of antioxidant benefits because of the flavonoid components. However, they also contain lectin, 7S α′ subunit, lipoxygenase, and Kunitz trypsin inhibitor (KTI) proteins that can be allergenic and digestive inhibitors and reduce processing aptitude. Genetic removal of these four proteins is necessary in soybean breeding. Therefore, this study was conducted to select a new line with brown seed coat, green cotyledon, and tetra-null genotype (lecgy1lox1lox2lox3ti) for lectin, 7S α′ subunit, lipoxygenase, and KTI proteins in the mature seed. Five germplasms were used to create breeding population. From a total of 58 F2 plants, F2 plants with lele genotype were selected using a DNA marker, and F3 seeds with a brown seed coat, green cotyledon, and the absence of 7S α′ subunit protein were selected. Three lines (S1, S2, and S3) were developed. Genetic absence of lectin, 7S α′ subunit, lipoxygenase, and KTI proteins was confirmed in F6 seeds of the three lines, which had a brown seed coat, green cotyledons, and a white hilum. The 100 seed weights of the three lines were 26.4-30.9 g, which were lower than 36 g of the check cultivar - 'Chungja#3'. The new S2 line with 30.9 g hundred seed weight can be used as a parent to improve colored soybean cultivars without antinutritional factors such as lectin, 7S α′ subunit, lipoxygenase, and KTI proteins.

Production of Feather-Sexing Korean Native Commercial Chickens (깃털 성감별 상업용 토종 실용닭 생산)

  • Sea Hwan Sohn;Eun Jung Cho;Seul Gy Lee;Junho Lee;Suyong Jang;Kwijoong Yong
    • Korean Journal of Poultry Science
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    • v.51 no.2
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    • pp.65-71
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    • 2024
  • The feather-sexing method is widely used commercially for chick sex identification. However, for feather-sexing to be industrially practical, the early-feathering (EF) and late-feathering (LF) genes must existed within the foundation stock, a suitable feather-sexing lines must be established, and the accuracy of sex identification by feather-sexing must be ensured. Therefore, this study introduces the method of constructing the Korean native chickens (KNC) feather-sexing lines using EF and LF genes and evaluates the effectiveness of feather sex determination on commercial chicks produced from the constructed KNC lines. The results showed that both EF and LF chickens existed within the foundation stock, with the frequency of LF genes ranging from 0 to 0.205. In feather-sexing line establishment, the paternal strain of the grandparent stock (GPS) was fixed as EF (kk) for both sexes, while the maternal strain was composed of males with LF homozygotes (ZKZK) and females with EF (ZkW). Thus, in the parent stock (PS), male breeder had EF (ZkZk) and female breeder had LF (ZKW), resulting in chicks produced from their crosses having LF (ZKZk) for males and EF (ZkW) for females, allowing sex determination based on feather development. Additionally, to evaluate the effectiveness of feather-sexing for the produced commercial chicks, a study was conducted on 1,000 samples of the produced chicks to investigate the concordance between vent-sexing and feather-sexing, showing a matching rate of 93.1%.