• 제목/요약/키워드: 결정성 분석

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Production of L-Lactic Acid from Soluble Starch by Enterococcus sp. JA-27. (Enterococcus sp. JA-27에 의한 가용성 전분으로부터 L형 젖산의 생산)

  • 김경아;김미경;장경린;전홍기
    • Microbiology and Biotechnology Letters
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    • v.31 no.3
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    • pp.250-256
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    • 2003
  • Lactic acid bacteria with amylolytic and acid producing activities can ferment starch directly to lactic acid thereby producing a monomer for the production of biodegradable poly lactic acid (PLA). In this study, the strain producing L-lactic acid from soluble starch was isolated from Nuruk. The isolated strain was identified as Enterococcus sp. through its morphological, cultural, biochemical characteristics as well as the 16S rDNA sequence analysis, and named Enterococcus sp. JA-27. Enterococcus sp. JA-27 produced exclusively L-lactic acid from soluble starch as a carbon source. The optimal conditions for the maximum production of L-lactic acid from Enterococcus sp. JA-27 were 30 C, pH 8, 1.5 % soluble starch as a substrate and 3.5 % tryptone as a nitrogen source, 0.1 % $K_2$$HPO_4$, 0.04 % $MgSO_4$. $7H_2$O, 0.014 % $MnSO_4$$.$4$H_2O$, 0.004% $FeSO_4$$.$$7H_2$O. Batch and fed batch culture were carried out and the former was more effective. L-Lactic acid production in the optimum medium was significantly increased in a 7 L jar fermenter, where the maximum L-lactic acid concentration was 3 g/L. For the purification of lactic acid in fermented broth, two stage ionexchange column chromatographies were employed and finally identified by HPLC.

A Comparative Case Study of Factors Determining Documentary Films' Success :Focusing on "My Love, Don't Cross That River" and "Two Doors" (다큐멘터리영화의 성공을 결정짓는 요인에 관한 비교사례연구 -<님아, 그 강을 건너지 마오>와 <두 개의 문>을 중심으로)

  • Im, So-Yeon;Lee, Yun-Cheol
    • The Journal of the Korea Contents Association
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    • v.17 no.10
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    • pp.503-517
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    • 2017
  • After a few recent documentary films had commercial success, factors for their success are getting attention. This paper explores the factors of the financial success of two documentary films. "My Love, Don't Cross That River" gained extended popularity with its universal message, and "Two Doors" conveyed a poweful political message. This paper conducted a case study by inteviewing production directors and distribution managers. Analysis was based on the three categories suggested by Litman and Kohl(1989): creative sphere, distribution-scheduling release pattern, and marketing efforts. Findings present the common factors of the two movies' success in the following areas: persons and events showing a powerful reality, a message with new and fresh insight, artistic quality, distribution strategy with timely scheduling, release time (period), public relations, and advertising.. In addition, "My Love, Don't Cross That River" was helped by a sufficient budget for scheduling/releasing and advertising. Its wide-release stratergy worked as well. "Two Doors" was aided by the government support for release and by the special factor called 'Participating viewer'. By examining the factors of documentary films' commercial success, this study has an implication for the popularization of documentary movies by indicating how to strategically apply relevant factors.

A Critical Assessment of the Economical Value of Road on Housing Prices Using Contingent Valuation Method - focus on the road construction plan of 47th of Suwon si - (조건부가치추정법을 이용한 도로의 경제가치 추정에 관한 실증적 연구 - 수원시 47번 도로건설계획을 중심으로)

  • Park, Ki-Hak
    • Environmental and Resource Economics Review
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    • v.21 no.4
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    • pp.999-1024
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    • 2012
  • The Purpose of this study was to assess the willingness to pay(WTP) for the new road construction plan(slip road of urban highway which was advertising extensively to reduce the time to the core of metropolitan area) on housing prices using contingent valuation method(CVM). And this study was an empirical study which was based on the consciousness of residents whose apartment was within the Suwon Si's new plan of 47th road construction zone. In this study, it was revealed that the resident's WTP for the plan of new road construction which was built on the neighborhood of apartment was within the 10 percent of housing prices that was similar to the standard declared price of MLTM(Ministry of Land, Transport and Maritime Affairs). And also revealed that the difference of sale prices of real estate company was 24 percent. The statistical assessment results show that both the resident's characteristic variables such as gender, incomes, size of apartment and the accessability variables such as distance to parks, schools, department stores, living facilities were positive effects on the prices of apartments significantly. Finally, the house has depreciated than that of MLTM and WTO since torpedoed the road construction plan. These results imply that, we have to take capital appreciation of the property into account due to road construction since the accessibility was very significantly positive internal effects on the apartment housing prices.

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Metallurgical Characteristics and Manufacturing Techniques of Ring-Pommel Swords Excavated from Ancient Tombs in Hadae, Ulsan (울산 하대고분 출토 민고리자루칼의 재질 특성과 환두부 제작 방법)

  • Jo, Ha Nui;Kim, Han Seul;You, Ha Rim;Lee, Jae Sung
    • Journal of Conservation Science
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    • v.36 no.3
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    • pp.197-212
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    • 2020
  • This study analyzed the microstructure of ring-pommel swords, excavated from Ancient Tombs, Hadae, Ulsan and examined their production technique, using non-destructive testing and a metallurgical method. The results confirmed that the five ring-pommel swords, unearthed in Ancient Tombs, Hadae, Ulsan, as identified by radiographic non-destructive testing, had been solely manufactured using iron, through forging based on the single-piece technique. Furthermore, these results were compared with previous studies, and the manufacturing techniques of single-piece ring-pommel swords were categorized into three types: pure iron - changing the shape, pure iron - changing the shape - carburization, and steel - changing the shape - quenching. The ring-pommels of four swords had around 0.7% of carbon content, which is as much as for eutectoid steel and higher than for other parts of these swords, such as the backs of their blades and handles. The weapon function of a small ring-pommel sword, under 60cm in length, was maximized by quenching focusing on its blade. Conversely, the martensite quenching structure was not observed in four ring-pommel swords shorter than 75cm. In other words, the same types of single-piece ring-pommel swords(late in 2C~early in 4C) were unearthed from Ancient Tombs, Hadae, and the group who has manufactured these swords is presumed to have limited their effectiveness, functionally depending on purposes, through an iron-making process and heat-treatment techniques.

A Statistical Analysis of the Seismicity of the Yangsan Fault System (양산단층계 지진활동의 통계적 분석)

  • 이기화;이전희;경재복
    • The Journal of Engineering Geology
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    • v.8 no.2
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    • pp.99-114
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    • 1998
  • The Yangsan fault system of Kyungsang Basin in the southeastern part of Korean peninsula is one of the most important structures in the peninsula. A number of strong earthquakes occurred in the vicinity of the fault. It was suggested that this fault can be divided into three segments: northern, central and southern ones. Earthquake data around the Yangsan fault were classified into two groups as incomplete and complete ones; the former is the data before the Choseon Dynasty and the latter is those since the dynasty. The maximum likelihood method was applied to compute seismicity parameters such as earthquake occurrence rates, b-values of frequency-magnitude relation and maximum possible magnitudes for each segment and the entire fault. These parameters show considerably different values from segment to segment. The b-value for the entire fault turned out to be 0.85 and maximum possible magnitudes for the northern, central and southern segments are 5.2, 6.8 and 6.0, respectively. The mean return periods for the maximum possible magnitudes for each segments are greater than 1000 years. In addition, according to the analysis of the frequency-magnitude relation, the occurrence pattern of earthquakes around the Yangsan fault show more similarity to the characteristic earthquake model than the Gutenberg-Richter model. The data for each segments are, however, too scarce to obtain any physically meaningful results.

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Development of Questionnaire for Automobile Seat Comfort Evaluation (자동차 시트의 안락도 평가를 위한 문항개발에 관한 연구)

  • Kim, Jung-A;Na, Ho-Jun;Cho, Dong-Hwan;Shin, Yun-Ho;Park, Se-Jin;Kim, Jin-Ho
    • Science of Emotion and Sensibility
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    • v.13 no.2
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    • pp.381-390
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    • 2010
  • The automotive seat comfort evaluation was to begin a key aspect in seat design. It depended largely on the basic mechanical aspect such as geometric parameters of seat, choice of suspension system and cushion material used. Until recently, seat comfort evaluation advanced to evaluate subjective sensitivity of human. The external literatures showed in the last decade, there have been very few attempts to establish and document automotive seat comfort evaluation. In 2006 Smith, D. proposed the statistically reliable tool in giving a numeric rating for set comfort and the tool was used in the many country. On the other hands, we, in Korea, had not the reliable tool for the automotive seat comfort evaluation. So that, we studied to develope the questionnaire for seat comfort evaluation based on Smith, D.(2006) and some studies. As a result, we developed 36 contents for the automotive seat comfort evaluation with the help of professional in Korean automotive industry. Here, 36 contents were identified as the dimensions that represent the human sensitivity and psychological feeling on comfortable seat. Also, we derived the priorities for the 36 contents by using analytic hierarchy process (AHP), based on the judgments of 30 experts and drivers. This study will help the designers and developers clarify the conceptual and abstract aspect of the design evaluation by proposing a more systematic and process-oriented method.

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Computer-Aided Diagnosis for Liver Cirrhosis using Texture features Information Analysis in Computed Tomography (컴퓨터단층영상에서 TIA를 이용한 간경화의 컴퓨터보조진단)

  • Kim, Chang-Soo;Ko, Seong-Jin;Kang, Se-Sik;Kim, Jung-Hoon;Kim, Dong-Hyun;Choi, Seok-Yoon
    • The Journal of the Korea Contents Association
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    • v.12 no.4
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    • pp.358-366
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    • 2012
  • Cirrhosis is a consequence of chronic liver disease characterized by replacement of liver tissue by fibrosis, scar tissue and regenerative nodules leading to loss of liver function. Liver Cirrhosis is most commonly caused by alcoholism, hepatitis B and C, and fatty liver disease, but has many other possible causes. Some cases are idiopathic disease from unknown cause. Abdomen of liver Computed tomography(CT) is one of the primary imaging procedures for evaluating liver disease such as liver cirrhosis, Alcoholic liver disease(ALD), cancer, and interval changes because it is economical and easy to use. The purpose of this study is to detect technique for computer-aided diagnosis(CAD) to identify liver cirrhosis in abdomen CT. We experimented on the principal components analysis(PCA) algorithm in the other method and suggested texture information analysis(TIA). Forty clinical cases involving a total of 634 CT sectional images were used in this study. Liver cirrhosis was detected by PCA method(detection rate of 35%), and by TIA methods(detection rate of 100%-AGI, TM, MU, EN). Our present results show that our method can be regarded as a technique for CAD systems to detect liver cirrhosis in CT liver images.

Development of a back analysis program for reasonable derivation of tunnel design parameters (합리적인 터널설계정수 산정을 위한 역해석 프로그램 개발)

  • Kim, Young-Joon;Lee, Yong-Joo
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.15 no.3
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    • pp.357-373
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    • 2013
  • In this paper, a back analysis program for analyzing the behavior of tunnel-ground system and evaluating the material properties and tunnel design parameters was developed. This program was designed to be able to implement the back analysis of underground structure by combination of using FLAC and optimized algorithm as direct method. In particular, Rosenbrock method which is able to do direct search without obtaining differential coefficient was adopted for the back analysis algorithm among optimization methods. This back analysis program was applied to the site to evaluate the design parameters. The back analysis was carried out using field measurement results from 5 sites. In the course of back analysis, nonlinear regression analysis was carried out to identify the optimum function of the measured ground displacement. Exponential function and fractional function were used for the regression analysis and total displacement calculated by optimum function was used as the back analysis input data. As a result, displacement recalculated through the back analysis using measured displacement of the structure showed 4.5% of error factor comparing to the measured data. Hence, the program developed in this study proved to be effectively applicable to tunnel analysis.

Assessment of Viral Attenuation in Soil Using Probabilistic Quantitative Model (확률적 정량모델을 이용한 토양에서의 바이러스 저감 평가)

  • Park, Jeong-Ann;Kim, Jae-Hyun;Lee, In;Kim, Song-Bae
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.7
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    • pp.544-551
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    • 2011
  • The objective of this study was to analyze VIRULO model, a probabilistic quantitative model, which had been developed by US Environmental Protection Agency. The model could assess the viral attenuation capacity of soil as hydrogeologic barrier using Monte Carlo simulation. The governing equations used in the model were composed of unsaturated flow equations and viral transport equations. Among the model parameters, those related to water flow for 11 soil types were from UNDODA data, and those related to 5 virus species were from the literatures. The model compared the attenuation factor with threshold of attenuation to determine the probability of failure and presented the exceedances and Monte Carlo runs as output. The analysis indicated that among 11 USDA soil types, the viral attenuation capacity of loamy sand and sand were far lower than those of clay and silt soils. Also, there were differences in the attenuation in soil among 5 viruses with poliovirus showing the highest attenuation. The viral attenuation capacity of soil decreased sharply with increasing soil water content and increased nonlinearly with increasing soil barrier length. This study indicates that VIRULO model could be considered as a useful screening tool for viral risk assessment in subsurface environment.

Fundamental Mechanisms of Platinum Catalyst for Oxygen Reduction Reaction in Fuel Cell: Density Functional Theory Approach (연료전지 산소환원반응 향상 위한 백금 촉매의 구조적 특성: 밀도범함수이론 연구)

  • Kang, Seok Ho;Lee, Chang-Mi;Lim, Dong-Hee
    • Journal of Korean Society of Environmental Engineers
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    • v.38 no.5
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    • pp.242-248
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    • 2016
  • The overall reaction rate of fuel cell is governed by oxygen reduction reaction (ORR) in the cathode due to its slowest reaction compared to the oxidation of hydrogen in the anode. The ORR efficiency can be readily evaluated by examining the adsorption strength of atomic oxygen on the surface of catalysts (i.e., known as a descriptor) and the adsorption energy can be controlled by transforming the surface geometry of catalysts. In the current study, the effect of the surface geometry of catalysts (i.e., strain effect) on the adsorption strength of atomic oxygen on platinum catalysts was analyzed by using density functional theory (DFT). The optimized lattice constant of Pt ($3.977{\AA}$) was increased and decreased by 1% to apply tensile and compressive strain to the Pt surface. Then the oxygen adsorption strengths on the modified Pt surfaces were compared and the electron charge density of the O-adsorbed Pt surfaces was analyzed. As the interatomic distance increased, the oxygen adsorption strength became stronger and the d-band center of the Pt surface atoms was shifted toward the Fermi level, implying that anti-bonding orbitals were shifted to the conduction band from the valence band (i.e., the anti-bonding between O and Pt was less likely formed). Consequently, enhanced ORR efficiency may be expected if the surface Pt-Pt distance can be reduced by approximately 2~4% compared to the pure Pt owing to the moderately controlled oxygen binding strength for improved ORR.