• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.3
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• pp.171-175
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• 2000

This study was carried out to obtain the basic informations on the flowering habit, pollination patterns and seed setting in Astragalus membranaceus. The first flowering appeared at the 20-22 node on main stem and there is the indeterminate inflorescence that the flowering progress developed toward the tip of the main stem and also toward the tips of branches. According to the flowering time, the number of peduncles were increased in early of Aug. Then, the number of bearing pods and that of seed setting were increased at that time. The length of peduncles with small flower was 6.6-7.3cm, and the number of small flowers were 13-17 per peduncles. The percentage of seed setting was 30-45% per peduncles and seeds were 4.8-5.3 per pod in peduncles. On 30 days after pollination, the pod was developed with the length about 3.8cm and the width about 8mm and the seeds were 7.3per pod. According to pollination treatment, wrapping sotted no seeds and the artificial pollination sotted seeds . about 5% in late of jul. to late of Aug. but increased about 13% of seed set after early of Sep. in A. membranaceus.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.27-27
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• 2018

본 연구는 강원지역에 분포하는 희귀 특산식물 5종 광릉요강꽃(CR), 개느삼(EN), 모데미풀(EN), 금강봄맞이(EN), 난장이붓꽃(EN)을 대상으로 고정조사구($5{\times}5m$, $2{\times}2m$, $1{\times}1m$)를 설치한 뒤, 2014년부터 2017년까지 4년간의 개화 및 결실 특성을 조사하여 자생지와 식물종의 보전을 위한 기초자료를 제공하기 위해 수행하였다. 그 결과, 광릉요강꽃의 개화율은 2014년 총 개체수 6개 중 100%(6개체), 2015년 6개 중 66.7%(4개체), 2016년 3개 중 33.3%(1개체), 2017년 7개 중 14.3%(1개체)으로 나타났고, 결실율은 2014년 0%(0개체), 2015년 33.3%(2개체), 2016년 0%(0개체), 2017년 0%(0개체)으로 각각 나타났다. 개느삼의 개화율은 2014년 총 개체수 83개 중 0%(0개체), 2015년 83개 중 1.2%(1개체), 2016년 83개 중 7.2%(6개체), 2017년 76개 중 0%(0개체)으로 나타났고, 2014년부터 2017년까지의 결실은 이루어지지 않은 것으로 조사되었다. 모데미풀의 개화율은 2014년 총 개체수 6개 중 33.3%(2개체), 2015년 23개 중 91.3%(21개체), 2016년 30개 중 50%(15개체), 2017년 16개 중 100%(16개체)으로 나타났고, 결실율은 2014년 33.3%(2개체), 2015년 52.2%(12개체), 2016년 36.7%(11개체), 2017년 37.5%(6개체)으로 각각 나타났다. 금강봄맞이의 개화율은 2014년 총 개체수 20개 중 50%(10개체), 2015년 27개 중 59.3%(16개체), 2016년 47개 중 34%(16개체), 2017년 58개 중 31%(18개체)으로 나타났고, 결실율은 2014년 50%(10개체), 2015년 29.6%(8개체), 2016년 4.3%(2개체), 2017년 10.3%(6개체)으로 각각 나타났다. 난장이붓꽃의 개화율은 2014년 총 개체수 28개 중 0%(0개체), 2015년 15개 중 100%(15개체), 2016년 28개 중 21.4%(6개체), 2017년 35개 중 62.9%(22개체)으로 나타났고, 2014년부터 2017년까지의 결실은 이루어지지 않은 것으로 조사되었다.

• Korean Journal of Agricultural and Forest Meteorology
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• v.4 no.3
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• pp.141-150
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• 2002

This study was conducted to reveal the effects of local climatic conditions on the yearly cone production in progeny test stand of Korean white pine. For this, yearly cone production by locality of progeny test stands was first measured and analyzed. The effects of climatic conditions on the cone production was analyzed by the estimation of yearly local climates based on both a topoclimatological method and a spatial statistical technique. From yearly climatic estimates, 19 climatic indices affecting cone production were computed for each of the progeny test stand. The yearly cone productions were then correlated with and regressed to the climatic indices to examine effects of local climatic conditions on the reproductive growth. According to correlation analysis, it was found that some typical climatic indices by locality were significantly correlated with the cone production. Also, the optimal regression equations which can estimate cone production by local climatic conditions were provided for applying to each of the progeny test stand of Korean white pine.

• Korean Journal of Agricultural and Forest Meteorology
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• v.3 no.4
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• pp.185-198
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• 2001

This study was conducted to reveal the effects of local climatic conditions on reproductive growth in a mature stand of Korean white pine based on climatic estimates. For this, the reproductive growth such as production and characteristics of cone and seed were first measured and summarized for seven years from 1974 to 1980. The local climatic conditions in the study site were also estimated by both a topoclimatological method and a spatial statistical technique. The local climatic conditions were then correlated with and regressed on the growth factors to reveal the relationships between the climatic estimates and the reproductive growth. Average number of conelet formation per tree showed highly negative correlation with some climatic variables related to minimum temperature in the year of flower bud differentiation. Especially, the most significant negative correlation were found between average of the minimum temperature for June and July of flower bud differentiation year and the number of conelet formation. There was no significant correlation between the number of cone production and climatic variables. However, total precipitation from December of the flowering year to February of the cone production year showed the most high correlation (r=0.6036) with the number of cone production. It was found that significant climatic variables affecting the amount of cone drop and cone drop percentage were the sum of cloudy days from June of the flowering year to August of the cone production year. Positive correlation was significantly recognized between the average weight of empty seed per cone and total precipitation from December of the flowering year to February of the cone production year. For the percentage of empty seed, five climatic variables among 19 variables were significantly correlated at 10% level. The average weight of a cone showed negative correlation with total precipitation from June of the flowering year to August of the cone production year. It was also found that average weight of a seed had highly negative correlation with total precipitation from December of the flowering year to February of the cone production year. The average weight of cone coat was negatively correlated with two climatic variables derived from clear days, which are sum of clear days from November of the flowering year to March of the cone production year and sum of clear days from December of the flowering year to February of the cone production year. On the other hand, it showed positive correlation with mean temperature of May in the flowering year. The exactly same results were obtained in correlation analysis for the percentage of cone coat.

• Journal of Sericultural and Entomological Science
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• v.52 no.1
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• pp.64-72
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• 2014

We bred a mulberry cultivar named Suhyang through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test was carried out at four places (Suwon, Kongju, Wanju and Jinju) for six years from 2007 to 2012. This is belonging to (Morus alba L.) crossed between Josaenghongpisang (Morus alba L.) and Kaeryang (Morus alba L.) among mulberry genetic resources preserved in Suwon. Suhyang was high yielding cultivar in fruit productivity by 12% compared to control cultivar 'Chungil (Morus alba L.)' for four years. Although Suhyang was higher in sugar content of mulberry fruits, it was bigger than that of 'Chungil'. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

• Journal of Sericultural and Entomological Science
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• v.51 no.2
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• pp.130-136
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• 2013

We bred a mulberry cultivar named Shimheungppong, through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at three places(Suwon, Wanju, and Jinju) for six years from 2004. This is tetraploidy variety belonging to (Morus alba L.) selected among mulberry genetic resources preserved in Suwon. Shimheungppong was high yielding cultivar in fruit productivity by 5% compared to control cultivar 'Chungilppong(Morus alba L.)' for four years. Although Shimheungppong was a little lower in sugar content of mulberry fruits, it was bigger than that of Chungilppong. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

• Korean Journal of Microbiology
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• v.42 no.2
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• pp.149-152
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• 2006

The sp%pl null mutant was constructed to study the function of fission yeast Schizosaccharomyces pombe spThp1, which is homologous to budding yeast Saccharomyces cerevisiae THP1. Tetrad analysis showed that the spThp1 is not essential for vegetative growth. The spThp1 null mutant also showed no massive poly(A)+ RNA export defect. However, spThp1 null is genetically associated with spMex67 null. These results suggest that spThp1 is involved in mRNA export out of the nucleus.

• Korean Journal of Microbiology
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• v.42 no.2
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• pp.153-155
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• 2006

We constructed the null mutants of fission yeast Schizosaccharomyces pombe spSac3 gene that is homologous to budding yeast Saccharomyces cerevisiae SAC3 involved in mRNA export out of nucleus. Tetrad analysis showed that the spSac3 is essential for vegetative growth. The spSac3 mutants harboring pREP81X-spSac3 plasmid showed poly(A)+ RNA export defect in the presence of thiamine. These results suggest that spSac3 is also involved in mRNA export from the nucleus.

• Journal of Genetic Medicine
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• v.5 no.1
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• pp.15-20
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• 2008

Purpose : Large exon deletions in the DMD gene are found in about 60% of DMD/BMD patients. Multiplex PCR has been employed to detect the deletion mutation, which frequently generates noise PCR products due to the presence of multiple primers in a single reaction as well as the stringency of PCR conditions. This often leads to a false-negative or false-positive result. To address this problematic issue, we introduced the dual primer oligonucleotide (DPO) system. DPO contains two separate priming regions joined by a polydeoxyinosine linker that results in high PCR specificity even under suboptimal PCR conditions. Methods : We tested 50 healthy male controls, 50 patients with deletion mutation as deletion-positive patient controls, and 20 patients with no deletions as deletion-negative patient controls using DPO-multiplex PCR. Both the presence and extent of deletion were verified by simplex PCR spanning the promoter region (PM) and 18 exons including exons 3, 4, 6, 8, 12, 13, 17, 19, 43-48, 50-52, and 60 in all 120 controls. Results : DPO-multiplex PCR showed 100% sensitivity and specificity for the detection a deletion. However, it showed 97.1% sensitivity and 100% specificity for determining the extent of deletions. Conclusion : The DPO-multiplex PCR method is a useful molecular test to detect large deletions of DMD for the diagnosis of patients with DMD/BMD because it is easy to perform, fast, and cost-effective and has excellent sensitivity and specificity.

• Korean Journal of Microbiology
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• v.54 no.1
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• pp.9-17
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• 2018

To investigate the role of bkdR, sigL, yplP, and des genes which were known to be involved in fatty acid synthesis and sensitive at low temperature, deletion mutants of Bacillus subtilis CU1065 and JH642 were constructed. To determine the low temperature sensitivity of these genes, we compared the growth curves of cells at $37^{\circ}C$ and $15^{\circ}C$. At $37^{\circ}C$, wild type and deletion mutants showed almost similar growth but only bkdR deletion strain at $15^{\circ}C$ showed very slow growing compared with wild type. At $15^{\circ}C$ sigL and yplP deletions were somewhat slower or similar to those of wild type strain. Double and triple mutants for bkdR, sigL, yplP deletions were constructed and grown at $20^{\circ}C$ in LB agar to investigate cold sensitive growth. Double or triple deletions including bkdR deletion showed cold sensitive growing. In order to identify more clearly cold sensitive growth, the experiments were carried out under cold shock conditions in which the temperature was lowered from $37^{\circ}C$ to $15^{\circ}C$ at the point of 0.4 optical densities at 600 nm. In these cold shock experiments, only bkdR deletion showed significantly lower growing and additional des deletion increases cold sensitivity. The bkdR activates the bkd operon, which catabolized isoleucine, valine and leucine, amino acids and produce precursors for the synthesis of branched fatty acids. At cold shock growing of bkdR deletion strain, isoleucine recovered cold sensitivity of bkdR deletion but valine did not restore cold sensitivity. Isoleucine is used as a precursor for the synthesis of anteiso-branched fatty acids. On the other hand, valine is used as a precursor for the synthesis of iso-branched fatty acids. This indicates that anteiso-branched fatty acid plays an important role at the cold shock condition.