• Title/Summary/Keyword: 검증시험

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Monitoring of illegal compounds and prohibited natural ingredients in foods (식품 중 발기부전치료제 및 사용금지 성분 모니터링)

  • Yun, Jisuk;Choi, Jangduck;Kwon, Kisung;Jo, Cheon-Ho
    • Korean Journal of Food Science and Technology
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    • v.48 no.5
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    • pp.405-412
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    • 2016
  • Illegally adulterated compounds, such as impotency drugs and their synthetic analogues, which have been purported to enhance sexual potency or mood, have been constantly detected in foods including dietary supplements. The adulterated foods with these illegal compounds may threaten public health because their safety and efficacy have not been verified. This study was aimed at investigating illegal compounds in foods and counterfeit products. 54 illegal compounds were assayed using a simultaneous analytical method involving liquid chromatography equipped with photo diode array (LC-PDA) and LC coupled with tandem mass spectrometry (LC-MS/MS). The method was validated in terms of selectivity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision and accuracy. In 48 of 161 samples, we identified 7 different illegal compounds, including sildenafil, tadalafil, chlropretadalafil, demethylsildenafil, dimethyl-thiosildenafil, icariin and yohimbine. When purchasing products marketed for erectile dysfunction or aphrodisiacs, ulmost care should be taken owing to the possible presence of these illegal compounds.

Screening of anti-obesity drugs, their analogues and prohibited ingredients in slimming foods (식품 중 부정 혼입된 비만치료제 및 사용금지 성분 실태조사)

  • Yun, Jisuk;Choi, Jangduck;Kwon, Kisung;Jo, Cheon-Ho
    • Korean Journal of Food Science and Technology
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    • v.48 no.5
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    • pp.424-429
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    • 2016
  • Globally, obesity has been recognized as a serious health problem. Recently, slimming foods for weight loss and maintenance were found to contain anti-obesity drugs, their analogues, and prohibited ingredients. To avoid inspections by the Government, structurally modified analogs have been continuously synthesized. For rapid determination of adulterated slimming products, we simultaneously analyzed 21 illegal compounds using liquid chromatography (LC) equipped with photo diode array, and LC coupled with tandem mass spectrometry. The validation of the method was performed with regard to selectivity, linearity, limit of detection, limit of quantification, precision, and accuracy. We purchased 128 samples from the Korean market and online sources in the year 2015. In 31 samples, 3 illegal compounds were detected as follows: 9.9-135.3 mg/g of sibutramine, 0.2-17.5 mg/g of yohimbine, and 1.8 mg/g of icariin. This simultaneous detection method might prove to be a simple and rapid analysis for monitoring illegal compounds in slimming foods.

Development and Validation of an Analytical Method for Glucuronolactone in Energy Drinks by Hydrophilic Interaction Liquid Chromatography-electrospray Tandem Mass Spectrometry

  • Oh, Mi Hyune;Lim, Moo Song;Chai, Jeung Young;Kim, Eun Jung;Cho, Joong Hoon;Lim, Chul Joo;Choi, Sun Ok
    • Journal of Food Hygiene and Safety
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    • v.32 no.2
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    • pp.89-95
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    • 2017
  • A rapid, sensitive analytical method for glucuronolactone in beverages was developed and validated using hydrophilic interaction liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HILIC-ESI-MS/MS). To determine the optimum analytical conditions for glucuronolactone, three different kinds of HILIC columns and two mobile phases with different pH values were examined. An amide-bonded stationary phase with a pH 9 acetonitrile-rich mobile phase was the best condition in terms of column retention, ESI-MS/MS response area, and signal-to-noise ratio. After extraction, glucuronolactone was separated through the HILIC amide column and detected by negative ESI-MS/MS in selected reaction monitoring (SRM) mode. Nine energy drinks sold in Korea were spiked with glucuronolactone at a concentration of 5 ng/mL; the Monster $Energy^{TM}$ sample showed the smallest peak area and its signal-to-noise ratio was used for method validation. Good linearity was obtained in the concentration range from 20 to 1500 ng/mL with a correlation coefficient > 0.998. The developed method had a limit of detection (LOD) of 6 ng/mL and a limit of quantitation (LOQ) of 20 ng/mL. The recovery of this method at concentration of 20, 100, 500, and 1000 ng/mL was 96.3%-99.2% with relative standard deviations (RSD) of 1.6%-14.0%. A reproducibility precision assessment at concentration of 100 and 500 ng/mL was carried out among three laboratories. The recovery of that evaluation was 95.1%-102.3% with RSD of 2.7%-7.0%. An analysis of variance indicated that there was no difference between the recovery results of the three laboratories at the 5% significance level. The validated method is applicable to inspecting beverages adulterated with glucuronolactone in Korea.

Inhibitory Effect of Various Cereal and Bean Extracts on Carcinogenicity in vitro (곡류 및 두류 에탄올 추출물의 in vitro 발암 억제 효과 비교)

  • Choi, Yeong-Hee;Kang, Mi-Young;Nam, Seok-Hyun
    • Korean Journal of Food Science and Technology
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    • v.30 no.4
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    • pp.964-969
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    • 1998
  • To investigated the anticarcinogenic activity of 70% ethanol extracts from various cereal in vitro, antimutagenic activity, inhibitory effect of DNA strand scission and tumor promotion were examined. The antimutagenic activity of the beans such as black bean and small red bean was generally higher than that of cereals examined. However inhibitory activity of 70% ethanolic extracts against DNA strand scission induced mitomycin C showed that millet, job's tear, black bean and soy bean among cereals and beans tested in this study inhibited effectively the DNA strand scission. Antioxidative activity of some cereal extracts determined by using linoleic acid model system showed that Job's tear, millet and black bean were higher antioxidative activity than other cereals and beans. Conventional short-term antipromoter assay system using activation of Epstein Barr virus (EBV) clearly demonstrated that sorghum, buckwheat, black bean and small red bean have inhibitory effects on promotion in cellular carcinogenesis.

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Implementation of a DB-Based Virtual File System for Lightweight IoT Clouds (경량 사물 인터넷 클라우드를 위한 DB 기반 가상 파일 시스템 구현)

  • Lee, Hyung-Bong;Kwon, Ki-Hyeon
    • KIPS Transactions on Computer and Communication Systems
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    • v.3 no.10
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    • pp.311-322
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    • 2014
  • IoT(Internet of Things) is a concept of connected internet pursuing direct access to devices or sensors in fused environment of personal, industrial and public area. In IoT environment, it is possible to access realtime data, and the data format and topology of devices are diverse. Also, there are bidirectional communications between users and devices to control actuators in IoT. In this point, IoT is different from the conventional internet in which data are produced by human desktops and gathered in server systems by way of one-sided simple internet communications. For the cloud or portal service of IoT, there should be a file management framework supporting systematic naming service and unified data access interface encompassing the variety of IoT things. This paper implements a DB-based virtual file system maintaining attributes of IoT things in a UNIX-styled file system view. Users who logged in the virtual shell are able to explore IoT things by navigating the virtual file system, and able to access IoT things directly via UNIX-styled file I O APIs. The implemented virtual file system is lightweight and flexible because it maintains only directory structure and descriptors for the distributed IoT things. The result of a test for the virtual shell primitives such as mkdir() or chdir() shows the smooth functionality of the virtual file system, Also, the exploring performance of the file system is better than that of Window file system in case of adopting a simple directory cache mechanism.

Experimental Study for Earthquake and Subsidence-resistant Performance Evaluation of iPVC Buried Water Pipe (iPVC 매립 상수도관의 내진 성능 및 내침하 성능 평가를 위한 시험적 연구)

  • Jeon, Bub-Gyu;Chang, Sung-Jin;Kim, Jae-Bong;Ju, Bu-Seog
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.24 no.1
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    • pp.16-23
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    • 2020
  • Water pipes are important facilities and consist of pipes of various specifications and materials. The annual average number of earthquakes in Korea is steadily increasing. Therefore, in case of the water pipe, it is estimated necessary to prepare for earthquakes. Damages to the water pipe by the earthquake can cause problems such as water supply and fire suppression, and cause damage to life and property. In Korea, however, it is difficult to find examples of seismic performance evaluation of water pipes based on experimental study. Damage to the water pipes by the earthquake is caused by the displacement-controlled behavior of the ground which is the liquifaction and fault lines. Especially, The damage to the water pipes by the earthquake is concentrated on the joint of the pipe. In particular, piping less than 200mm in diameter was found to be dangerous. Thus, in this study, the seismic and settlement performance of iPVC buried water pipes with fixed joints with a clamp of 150mm was evaluated with a test approach.

Evaluating Performance of Cable-Inspection Robot in Cable-Supported Bridge (케이블지지 교량의 케이블 점검 로봇 성능 평가)

  • Kim, Jaehwan;Seo, Dong-Woo;Jung, Kyu-San;Park, Ki-Tae
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.24 no.1
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    • pp.74-79
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    • 2020
  • Safety inspection of cable-supported bridge has increasingly attention as many cable-supported bridges are currently constructed/operated. Whilst cables as a main component in cable-supported bridge should be inspected regularly, traditional method (visual inspection) has limitation to check the condition of cables properly due to restricted factors. It is evidently necessary to develop cable-inspection robot to overcome this concern. In this respect, the main aim in this study is to manufacture the improved robot compared with the existing robot. The improved functions of the robot in this study were that the robot can be operated in large cable diameter (greater than 200 mm) and climbing ability of the robot increases. In addition, electro-magnetic sensor as a non-destructive method in the robot was added to detect damaged cables and performance of the sensor was evaluated in indoor and field experiments. Consequently, the robot was able to move on the cable with ~0.2m/s and to detect damaged cables using the sensor. It was also confirmed that performance of the robot in field test is similar to that in indoor test.

Study on the Air Insulation Design Guideline for ±500 kV Double Bipole Transmission Line with Metallic Return Conductor (도체귀로형 ±500 kV Double Bipole 송전선로 공기절연에 관한 연구)

  • Shin, Kooyong;Kwon, Gumin;Song, Seongwhan;Woo, Jungwook
    • KEPCO Journal on Electric Power and Energy
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    • v.5 no.3
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    • pp.141-147
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    • 2019
  • Recently, the biggest issue in the electricity industry is the increase in renewable energy, and various technologies are being developed to ensure the capacity of the power system. In addition, super-grids linking power systems are being pushed to utilize eco-friendly energy between countries and regions worldwide. The HVDC transmission technology is required to link the power network between regions with different characteristics of the power system such as frequency and voltage. Until now, Korea has applied HVDC transmission technology that connects mainland and Jeju Island with submarine cables. But, the HVDC transmission technology is still developing for long-distance high-capacity power transmission from power parks on the east coast to load-tight areas near the metropolitan area. Considering the high population density and mountainous domestic environment, it is pushing for commercialization of the design technology of the ${\pm}500kV$ Double Bipole with metallic return wire transmission line to transmit large-scale power of 8 GW using minimal right of ways. In this paper, the insulation characteristics were studied for the design of double-bipole transmission tower with metallic return wire, which is the first time in the world. And the air insulation characteristics resistant to the various overvoltage phenomena occurring on transmission lines were verified through a full-scale impulse voltage test.

Analysis Model for Design Based on Stiffness Requirement of Direct Drive Electromechanical Actuator (직구동 전기기계식 구동기의 강성요구규격에 기반한 설계용 해석모델)

  • Oh, Sang Gwan;Lee, Hee Joong;Park, Hyun Jong;Oh, Dongho
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.47 no.10
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    • pp.738-746
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    • 2019
  • Instead of hydraulic actuation systems, an electromechanical actuation system is more efficient in terms of weight, cost, and test evaluation in the thrust vector control of the 7-ton gimbal engine used in the Korea Space Launch Vehicle-II(KSLV-II) $3^{rd}$ stage. The electromechanical actuator is a kind of servo actuator with position feedback and uses a BLDC motor that can operate at high vacuum. In the case of the gimballed rocket engine, a synthetic resonance phenomenon may occur due to a combination of a vibration mode of the actuator itself, a bending mode of the launcher structure, and an inertial load of the gimbals engine. When the synthetic resonance occurs, the control of the rocket attitude becomes unstable. Therefore, the requirements for the stiffness have been applied in consideration of the gimbal engine characteristics, the support structure, and the actuating system. For the 7-ton gimbal engine of the KSLV-II $3^{rd}$ stage, the stiffness requirement of the actuation system is $3.94{\times}10^7N/m$, and the direct drive type electromechanical actuator is designed to satisfy this requirement. In this paper, an equivalent stiffness analysis model of a direct drive electromechanical actuator designed based on the stiffness requirements is proposed and verified by experimental results.

Relationship between the P Wave Velocity, Static Elastic Modulus, and Dynamic Elastic Modulus of Rocks (암종별 P파 속도, 정탄성계수 및 동탄성계수의 상관관계 분석 연구)

  • Moon, Seong-Woo;Kim, Hyeong-Sin;Yun, Hyun-Seok;Seo, Yong-Seok
    • The Journal of Engineering Geology
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    • v.29 no.3
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    • pp.339-349
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    • 2019
  • The relationship between the P wave velocity, static elastic modulus, and dynamic elastic modulus of different rock types was investigated to identify the distributive characteristics of the dynamic elastic modulus. Laboratory and in situ test results from 1,646 rock specimens, which are obtained for design and construction of structure, were analyzed, and grouped into three key rock types: gneiss, granite, and sandstone. These relationships were verified by comparing them with the results from previous studies. The gneiss samples exhibit a linear P wave velocity-static elastic modulus relationship, whereas the granite and sandstone samples exhibit exponential relationships. Their coefficient of determination ($R^2$) values are all in the 0.491-0.642 range, and are similar to those obtained in previous studies. The relationship between the static and dynamic elastic modulus exhibits a linear relationship for all rock types, yielding a coefficient of determination in the 0.543-0.676 range. The relationship between the P wave velocity and static elastic modulus follows an exponential regression for all rock types, with a high coefficient of determination that is in the 0.875-0.940 range.