• Korean Journal of Plant Resources
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• v.28 no.2
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• pp.158-167
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• 2015

We selected 8 plants among 11 Jeju native plants to search useful natural anti-oxidants by determining the amount of total polyphenols and the various anti-oxidative effects. Ethyl acetate extracts of Castanopsis sieboldii (Makino) Hatus. and butanol extracts of Oenothera laciniata Hill showed strong DPPH free radical scavenging effect. The IC₅₀ value of each solvent extract was 1.6 ㎍/㎖ and 2.4 ㎍/㎖, respectively. The ethyl acetate fraction of Castanea crenata Siebold & Zucc exhibited strong inhibition against nitric oxide production. For the inhibition of xanthine oxidase, the ethyl acetate extracts of Castanea crenata Siebold & Zucc showed strong inhibition activity with 16 ㎍/㎖ of its IC₅₀. The ethyl acetate extracts from Castanopsis sieboldii (Makino) Hatus showed strong superoxide scavenging effect with 7 ㎍/㎖ of its IC₅₀. The hydroxyl radical scavenging activity of butanol extract of Castanopsis sieboldii (Makino) Hatus was 76%. Therefore, with more researches on purification and identification of active compounds, plants studied are expected to be natural sources for the functional food/cosmeceuticals with anti-oxidative properties.

• Journal of the Korean Society for Computer Game
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• v.31 no.4
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• pp.157-166
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• 2018

Communication between customers and service providers is an important issue at sites where various businesses and transactions take place. In particular, the ability to solve problems quickly and accurately when a problem arises and when an inquiry is received is directly linked to trust in the site. In this paper, we propose a method of handling complaints and inquiries of site users by using chatbot technology on talent market platform site. First, we implemented chatbot that can communicate with the inquirers in real time, so that users can use the site usage and word search functions. For various errors and problems of the site which can not be defined by a few words or sentences, I have specified an error code and database it. Users of the site were able to contact chatbot with the error code that was output when an error occurred and get the corresponding response in real time. The chatbot implemented in this study provided a satisfactory experience because that was able to provide quick and accurate answers to users who experienced errors or inquiries when using the site. This will have a positive impact on the credibility and favorability of the site over the long term, and will help reduce manpower and time costs for error inquiries.

• Land and Housing Review
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• v.12 no.2
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• pp.121-139
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• 2021

Technical documents in the construction field are changing from paper documents to electronic ones. As a result, the industry witnesses a trend of using portable electronic devices in searching or retrieving necessary information such as relevant regulations. Despite the improvement in the accessibility to general technical documents, a limitation is still found in accessing the electronic documents on the regulations. We see the barrier for field engineers to enhance their technical knowledge. One of major barriers is that videos, animations, and virtual reality information to enhance the visual understanding of technical content related to regulations are not linked. It is the interactive electronic technical manual (IETM) that can address such issues. The IETM is an electronic document system that enables real-time information acquisition while operating in the form of conversations with users by linking multimedia functions to document types such as specifications and guidelines. This study establishes a model of the IETM that can be operated in the planning stage of a construction project. The study also verifies its usability with a hypothetical case study. This study aims to improve the usability of the IETM in the construction project by analyzing the application effect of the IETM using the AHP technique.

• Korean Journal of Breeding Science
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• v.43 no.5
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• pp.448-456
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• 2011

We used suppression subtractive hybridization (SSH) combined with mirror orientation selection (MOS) method to screen differentially expressed genes from red-fleshed kiwifruit 'Hongyang'. As a result, the 288 clones were obtained by subcloning PCR product and 192 clones that showed positive clones on colony PCR analysis were selected. All the positive clones were sequenced. After comparisons with the NCBI/Genbank database using the BLAST search revealed that 30 clones showed sequence similarity to genes from other organisms; 10 clones showed significant sequence similarity to known genes. Among these clones, 3 clones (AcF21, AcF42 and AcF106) had sequence homology to 1-aminicyclopropane-carboxylic acid (ACC)-oxidase (ACO) that known to be related to fruit ripening. The expression patterns of differentially expressed genes were further investigated to validate the SSH data by reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qReal-time PCR) analysis. All the data from qReal-time PCR analysis coincide with the results obtained from RT-PCR analysis. Three clones were expressed at higher levels in 'Hongyang' than 'Hayward'. AcF21 was highly expressed in the other genes at 120 days after full bloom (DAFB) and 160 DAFB of 'Hongyang'.

• Korean Journal of Breeding Science
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• v.40 no.4
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• pp.387-393
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• 2008

Over 7 individual rice (Oryza sativa L.) plants per a line were sowed and sampled by pooled sampling method for genomic DNA extraction. The 5,400 flanking sequence tags (FSTs) were analysed by adaptor PCR and direct sequencing. FST analysis showed that the intragenic FSTs, the intergenic FSTs, and the original insertional sequences including hot spot covered 48.1% (2,597), 25.6% (1,383), and 25% (1,350), respectively. The 2,597 intragenic FSTs were used for genotyping to determine whether they are heterozygous or homozygous, and 1,393 core lines were selected. Among them, 422 knockout genes were distributed on chromosome 3, while 56 - 157 intragenic FSTs scattered on other chromosomes. Among 1,393 FSTs, known genes such as transcription factor covered 59.4% (827), while unknown genes such as expressed protein covered 40.6% (566). RT-PCR indicated that some core lines had no expression or decreased expression level in their knockout genes. It means that core lines are very useful knockout lines for functional genomic studies.

• Journal of Plant Biotechnology
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• v.45 no.4
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• pp.289-298
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• 2018

The Biosafety Division of the National Academy of Agricultural Science has developed a 'Crop Composition DB' that provides analytical data on commercialized crops. It can be used as a reference in the 'Comparative Evaluation by Compositional Analysis' for the safety assessment of genetically modified (GM) crops. This database provides the composition of crops cultivated in Korea, and thus upgrades the data to check the extent of changes in the compositional content depending on the cultivated area, varieties and year. The database is a compilation of data on the antioxidant, nutrient and secondary metabolite compositions of rice and capsicum grown in two or more cultivation areas for a period of more than two years. Data analysis was conducted under the guidelines of the Association of Official Analytical Chemists or methods previously reported on papers. The data was provided as average, minimum and maximum values to assess whether the statistical differences between the GM crops and comparative non-GM crops fall within the biological differences or tolerances of the existing commercial crops. The Crop Composition DB is an open-access source and is easy to access based on the query selected by the user. Moreover, functional ingredients of colored crops, such as potatoes, sweet potatoes and cauliflowers, were provided so that food information can be used and utilized by general consumers. This paper introduces the feature and usage of 'Crop Composition DB', which is a valuable tool for characterizing the composition of conventional crops.

• Journal of Digital Convergence
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• v.17 no.6
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• pp.267-277
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• 2019

The purpose of this study is to investigate the burden of caring for the care-givers of the elderly in Korea by using an integrated literature review method. A total of 23 studies were analyzed using a search database. When care-givers had higher sense of filial and guilt or more than two diseases, they showed high level of feeling of burden. Also, they had high level of feeling of physical burden by their oldness, service period (especially at the period of 1-3 years). In the feeling of economic burden, they had high level of feeling of burden by their oldness, or elderly's disease periods. The feeling of burden by psychological condition was found in elderly in aged and the beginning of admission of nursing home. The feeling of burden by environment situation was found when the functional status of the elderly was bad. Therefore, we need to concern care-givers's feeling of burden with elderly people in the nursing home. In the future, I believe that the findings of this study will be helpful for development of the intervention program for alleviate burden for the care-giver.

• The Journal of the Korea Contents Association
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• v.19 no.2
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• pp.11-21
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• 2019

It has been about 20 years since the English subject was formally taught in public elementary schools in Korea. The present research aims to analyze the studies regarding 'primary English' implemented in Korea during the time period. I have investigated 6,467 theses or research papers in total that were published in Korea with the help of the corpus programs Utagger and WordSmith Tools. The results show that for the last 20 years the number of overall studies appears to have increased since the year 1997, although the recent trend seems to be in recession. The research scope ranges from 'teaching-learning interaction' to 'curriculum' and 'assessment', which have been steadily investigated for 20 years. Furthermore, researchers sometimes appear to have followed the English education policy by conducting particular investigations like 'immersion program' or 'native English speaking teachers' in a certain time period. Recently, researchers started to have interest in the cutting-edge ICT. In conclusion, the academic field of 'primary English' in Korea has grown in quantity, and the spectrum of research areas has been expanded for the past 20 years. It is hoped that the results of this research will help set a new direction for future research.

• Journal of Life Science
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• v.28 no.11
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• pp.1379-1385
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• 2018

Glycans are attached to proteins as in glycoproteins and proteoglycans. They are found on the exterior surface of cells. O- and N-linked glycans are very common in eukaryotic cells but may also be found in prokaryotes. The interaction of cell surface glycans with complementary glycan binding proteins located on neighboring cells, other cell types, pathogens like virus, or bacteria is crucial in biologically and biomedically important processes like pathogen recognition, cell migration, cell-cell adhesion, development, and infection. Their implication in pathological condition, suggests an important role for glycans as disease markers. In addition, a great amount of research has been shown that appropriate glycosylation of a recombinant therapeutic protein is critical for product solubility, stability, pharmacokinetics and pharmacodynamics, bioactivity, and safety. Besides, cancer-associated glycosylation changes often involve sialic acid in glycan branch which play important roles in cell-cell interaction, recognition and immunological response. This review aims at giving a comprehensive overview of the glycan's biological function and describing the relevance among the glycosylation, disease diagnosis and treatment methods. Furthermore, the high-throughput analytic methods available to measure the profile changing patterns of glycan in the blood serum as well as possible underlying biochemical mechanisms.

• Convergence Security Journal
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• v.21 no.3
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• pp.75-80
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• 2021

This study provided personnel and recruitment information related to job offer and job hunting, and proposed a non-face-to-face platform that can facilitate matching of one-time services as well as periodic services. The existing recruitment and job search method for personal assistant of the disabled is a one-way matching method in which a person in charge of the service providing centers assigns an activity assistant belonging to the institution when a consumer requests an personal assistant service, so the choice of job seekers is very weak. The job matching platform of the activity assistant for the disabled can be logged in separately by dividing users and separating them into the interface of the disabled or the disabled family members (job seekers) and activity assistants for the disabled (job seekers). Search for job offer and job seekers that meet their respective conditions, and real-time bidirectional decision-making through the chat function, and when the activity assistance service is completed, recruiters and job seekers can write service reviews and evaluations from their own point of view. The real-time interactive decision-making matching platform will also help minimize blind spots where it is difficult to provide services when short-term or one-time assistance is needed.