• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.424-433
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• 2018

Watermelon (Citrullus lanatus) is an economically important vegetable crop all over the world, which has functional compounds such as lycopene and citrulline. Gummy stem blight caused by Didymella bryoniae is one of the most devastative diseases in watermelon. Single nucleotide polymorphisms (SNPs), which are genetic variations occurring between individuals with respect to a single base, were often used to construct genetic linkage maps and develop molecular markers linked to a variety of horticultural traits and resistance to several diseases. In this study, we developed high-resolution melting (HRM) markers based on SNPs generated from NGS resequencing of two parents in watermelon. Plant materials were C. lanatus '920533' (female and susceptible parent), C. amarus 'PI 189225' (male and resistant parent), and their $F_1$ and $F_2$ progenies. A total of 13.6 Gbp ('920533') and 13.1 Gbp ('PI 189225') of genomic sequences were obtained using NGS analysis. A total of 6.09 million SNPs between '920533' and 'PI 189225' were detected, and 354,860 SNPs were identified as potential HRM primer sets. From these, a total of 330 primer sets for HRM analysis were designed. As a result, a total of 61 HRM markers that have polymorphic melting curves were developed. These HRM markers can be used for the construction of SNP-based linkage maps and for the analysis of quantitative trait loci (QTLs) related to gummy stem blight resistance.

• Journal of Science Education
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• v.47 no.2
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• pp.192-210
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• 2023

This study was intended to develop the plant molecular biology experimental program using Rapid-cycling Brassica rapa (RcBr) based on the teaching steps and teaching methods of the cognitive apprenticeship model and to determine its application effects. In order to improve a subject's cognitive function and expertise on molecular biology experiments, two themes composed of a total 8 class sessions were selected: 'Identification of DFR gene in purple RcBr and non-purple RcBr' and 'Identification of RcBr's genetic polymorphism site using the DNA profiling method'. Research subjects were 18 pre-service teaching majors in biology education of H University in Chungbuk, Korea. The effectiveness of the developed program was verified by analyzing the enhancement of 'cognitive function' related to the use of molecular biology knowledge and technology, and the enhancement of 'domain-general metacognitive abilities.' The effect of the developed program was also determined by analyzing the task flow diagram provided. The developed program was effective in improving the cognitive functions of the pre-service teachers on the use of knowledge and technology of molecular biology experiments. It was especially effective to improve the higher cognitive function of pre-service teachers who did not have the previous experience. The developed program also showed a significant improvement in the task of metacognitive knowledge and in the planning, checking, and evaluation of metacognitive regulation, which are sub-elements of domain-general metacognitive abilities. It was found that the developed program's self-test activity could help the pre-service teachers to improve their metacognitive regulation. Therefore, this developed program turned out to be helpful for pre-service teachers to develop core competencies needed for molecular biology experimental classes. If the teaching and learning materials of the developed program could be reconstructed and applied to in-service teachers or high school students, it would be expected to improve their metacognitive abilities.

• Korean Journal of Plant Resources
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• v.36 no.5
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• pp.496-507
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• 2023

The in vitro organogenesis is one of important issues in plant embryology, and somaclonal variations are existing in calli and/or regenerants induced from a process of the organogenesis with in vitro circumstances. In this study, expressions of organogenesis-related genes were evaluated and genetic stability of regenerants derived from the process of in vitro organogenesis were measured using ISSR markers in Imperata cylindrica 'Rubra', Poaceae. The expressions of organogenesis-related genes were detected all of regenerants at the process of the organogenesis. All ISSR markers produced with an average of 71 bands per in vitro-cultured regenerants, and the scorable bands were varied from two to eight with an average of 5.14 bands per a primer. The polymorphism rates of the in vitro regenerants were higher than that of mother plants (1.4%), showing 4.1% (pot-cultured regenerants), 4.3% (field-cultured regenerants), 4.2% (in vitro-cultured regenerants), 5.6% (calli with green shoots) and 1.4% (calli), respectively. The genetic similarity matrix (GSM) among all accessions ranged from 0.747 to 1.0 with a mean of 0.868. GSM of the regenerants showed differences (from 0.972 to 1.00) compared with that of mother plants (0.991). According to the clustering analysis, two independent groups were divided into; the one is mother plants and regenerants cultured at room and open field, the other is regenerants cultured in vitro. The results give a new insight for understanding the dynamics of organogenesis in monocot plant.

• Korean Journal of Ichthyology
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• v.27 no.1
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• pp.10-15
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• 2015

Giant grouper (Epinephelus lanceolatus) is a endangered species considered as a vulnerable grade-organism in the International Union for Conservation of Nature (IUCN) red list. As a fundamental baseline study for establishing a giant grouper broodstock management system, the efficiency for parentage analysis was evaluated by using microsatellite makers previously available in this species. The eight microsatellites generated a total 52 alleles from 32 individuals, the mean expected heterozygosity was 0.663, and mean inbreeding coefficient was 0.011, consequently suggesting that the present broodstock has retained the high level of genetic diversity. However, our analysis also recommended the collection of more broodfish for more stable brood line, since the estimated value of the effective population size was proven to be 35. The average probability of identity was $6.85{\times}10^{-11}$. NE-2P and NE-PP of paternity non-exclusion probabilities were 0.00835 and 0.00027, respectively. As the result of principle coordinate analysis, the genotype of broodstock was not overlapped, suggesting that the management system of giant grouper based on eight selected microsatellite markers might be effective, although further validation with extended number of broodfish might also be needed in future. Data of present study could be a useful basis to avoid the unwanted selection of broodfish that possess close genetic relationship with current broodstock, and consequently to establish effective broodstock management system allowing the production of progeny with high genetic diversity.

• Journal of Intelligence and Information Systems
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• v.24 no.3
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• pp.243-262
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• 2018

Database schema integration is a significant issue in information systems. Because schema integration is a time-consuming and labor-intensive task, many studies have attempted to automate it. Researchers typically use XML as the source schema and leave much of the work to be done through DBA intervention, e.g., there are various naming conflicts related to relationship names in schema integration. In the past, the DBA had to intervene to resolve the naming-conflict name. In this paper, we introduce an algorithm that automatically generates relationship names to resolve relationship name conflicts that occur during schema integration. This algorithm is based on an Internet collocation and English sentence example dictionary. The relationship between the two entities is generated by analyzing examples extracted based on dictionary data through natural language processing. By building a semi-automated schema integration system and testing this algorithm, we found that it showed about 90% accuracy. Using this algorithm, we can resolve the problems related to naming conflicts that occur at schema integration automatically without DBA intervention.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.42 no.5
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• pp.41-48
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• 2005

OSGi(Open Service Gateway Initiative) offers a unique opportunity for pervasive computing as a potential framework for achieving interoperability between various sensors, home appliances, and networked devices. The OSGi framework supports a remote installation of a bundle, which is a unit that installs and deploys services. However, in order for the bundle in execution to migrate, a specific form of bundle such a mobile agent, is needed one which is able to move through a heterogeneous network. This paper proposes a method that can manage bundles like these dynamic agents, in order to ensure the mobility of entities in multiple OSGi framework environments. For our purposes, we have designed the mobile agent management system for managing the lifecycle and mobility of agents in the OSGi framework. The mobile agent management system we are proposing implements a bundle form which can perform in an OSGi framework as well as manage autonomous mobile services. As a result, mobility in a pervasive environment will be supported more efficiently.

• Proceedings of the Korea Information Processing Society Conference
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• 2003.05c
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• pp.1651-1654
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• 2003

이동 단말의 보급이 보편화됨에 따라 이동 객체의 위치 정보를 기반으로 사용자에게 사람이나 사물, 차량 등과 같은 이동 객체의 위치를 파악하여 그에 대한 정보를 제공해 주는 시스템이 필요로 하게 되었나 이러만 이동 객체관리 시스템에서는 계속적으로 위치 정보가 변화하는 이동 객체의 특성상 데이터의 빈번한 갱신이 일어나게 되고 DBMS에 명시적으로 저장되지 않은 위치 정보에 대해서도 보다 정확한 위치를 사용자에게 제공해 주어야 한다. 그러나 차량의 위치 추적과 같이 적용 개체가 차량에 한정된 경우 이동 경로가 도로상으로 제한되어 있으므로 이동 경로를 예측하기 힘든 사람과 같은 객체와는 특성이 나르나 따라서 차량 객체에 대해 보다 효과적인 서비스를 제공해 주기 위해서는 사람에 대한 위치 추적과는 다른 갱신 정책과 불확실한 위치의 추정 기법이 필요하다. 본 논문에서는 공간 데이터에 저장된 도로의 위상 정보와 차량의 속도 속성을 이용한 갱신 정책을 정하여 갱신 빈도수로 줄이고 도로 레이어의 위상 정보를 통해 불확실한 과거 및 미래의 위치로 추정하는 기법을 제안한다. 제안한 갱신 정책은 차량의 속도를 고려하여 현재의 위치에서 도로상의 교차점에 도착하는 시점의 위치를 예측하여 데이터의 갱신 시점으로 결정한다. 또한 불확실한 위치에 대한 추정은 이동하는 도회와 대응되는 위상 정보를 기반으로 차량의 이동 방향을 예측하 여 불확실한 미래의 위치를 결정할 수 있으며 명시적으로 저장되지 않은 과거 위치 정보의 검색에 대한 요청이 발생했을 경우 위상 정보를 이용하여 위치를 보정하고 사용자에게 보나 높은 정확성을 지닌 정보를 제공해 줄 수 있다.다. SQL Server 2000 그리고 LSF를 이용하였다. 그리고 구현 환경과 구성요소에 대한 수행 화면을 보였다.ool)을 사용하더라도 단순 다중 쓰레드 모델보다 더 많은 수의 클라이언트를 수용할 수 있는 장점이 있다. 이러한 결과를 바탕으로 본 연구팀에서 수행중인 MoIM-Messge서버의 네트워크 모듈로 다중 쓰레드 소켓폴링 모델을 적용하였다.n rate compared with conventional face recognition algorithms. 아니라 실내에서도 발생하고 있었다. 정량한 8개 화합물 각각과 총 휘발성 유기화합물의 스피어만 상관계수는 벤젠을 제외하고는 모두 유의하였다. 이중 톨루엔과 크실렌은 총 휘발성 유기화합물과 좋은 상관성 (톨루엔 0.76, 크실렌, 0.87)을 나타내었다. 이 연구는 톨루엔과 크실렌이 총 휘발성 유기화합물의 좋은 지표를 사용될 있고, 톨루엔, 에틸벤젠, 크실렌 등 많은 휘발성 유기화합물의 발생원은 실외뿐 아니라 실내에도 있음을 나타내고 있다.>10)의 $[^{18}F]F_2$를 얻었다. 결론: $^{18}O(p,n)^{18}F$ 핵반응을 이용하여 친전자성 방사성동위원소 $[^{18}F]F_2$를 생산하였다. 표적 챔버는 알루미늄으로 제작하였으며 본 연구에서 연구된 $[^{18}F]F_2$가스는 친핵성 치환반응으로 방사성동위원소를 도입하기 어려운 다양한 방사성의 약품개발에 유용하게 이용될 수 있을 것이다.었으나 움직임 보정 후 영상을 이용하여 비교한 경우, 결합능 변화가 선조체 영역에서 국한되어 나타나며

• Journal of the Korean Society for Railway
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• v.11 no.4
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• pp.349-356
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• 2008

Put Safety-critical systems related to the railway communications are currently undergoing changes. Mechanical and electro-mechanical devices are being replaced by programmable electronics that are often controlled remotely via communication networks. Therefore designers and operators now not only have to contend with component failures and user errors, but also with the possibility that malicious entities are seeking to disrupt the services provided by theirs systems. Recognizing the safety-critical nature of the types of communications required in rail control operations, the communications infrastructure will be required to meet a number of safety requirements such as system faults, user errors and the robustness in the presence of malicious attackers who are willing to take determined action to interfere in the correct operation of a system. This paper discusses the safety strategies employed in the railway communications and proposes a security mechanism for Korean railway communication system. We present the developed communication safety evaluation tool based on the proposed security mechanism and also evaluate its protecting capability against the threats of masquerading, eavesdropping, and unauthorized message manipulation.

• Journal of KIISE:Software and Applications
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• v.33 no.11
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• pp.986-998
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• 2006

There has been an increasing necessity of Semantic Web-based metadata that helps computers efficiently understand and manage an information increased with the growth of Internet. However, it seems inevitable to face some semantically ambiguous information when metadata is generated. Therefore, we need a solution to this problem. This paper proposes a new method for automated metadata generation with the help of a concept of class, in which some ambiguous words imbedded in information such as documents are semantically more related to others, by using probability model of consequent words. We considers ambiguities among defined concepts in ontology and uses the Hidden Markov Model to be aware of part of a named entity. First of all, we constrict a Markov Models a better understanding of the named entity of each class defined in ontology. Next, we generate the appropriate context from a text to understand the meaning of a semantically ambiguous word and solve the problem of ambiguities during generating metadata by searching the optimized the Markov Model corresponding to the sequence of words included in the context. We experiment with seven semantically ambiguous words that are extracted from computer science thesis. The experimental result demonstrates successful performance, the accuracy improved by about 18%, compared with SemTag, which has been known as an effective application for assigning a specific meaning to an ambiguous word based on its context.

• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.434-441
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• 2018

High-density genetic linkage mapping is critical for undertaking marker-assisted selection and confirming quantitative trait loci, as well as helping to build pseudomolecules of genomes. We constructed a genetic map using 94 $F_1$ populations generated from the interspecific cross between Korean cultivar "Wonwhang" (Pyrus pyrifolia, NCBI BioSample SAMN05196235) and European cultivar "Bartlett" (Pyrus communis). We designed a total of 24,267 SSR markers based on the genome sequences of "Wonwhang" for this. To select the markers that are linked to the traits important in pear breeding programs, SSR-containing genomic sequences were subjected to nucleotide sequence homology searches, which resulted in 510 SSR markers with high similarity to genes encoding proteins with putative functions such as transcription factors, resistance proteins, flowering time, and regulatory genes. Of these, 70 markers showed polymorphisms in parents and segregating populations and were used to construct a genetic linkage map, together with the unpublished 579 SNPs obtained from genotyping by sequencing analysis. The genetic linkage map covered 3,784.2 cM and the average distance between adjacent markers was 5.8 cM. Seventy SSR markers were distributed across 17 chromosomes with more than one locus.