• Development and Reproduction
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• v.9 no.2
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• pp.105-114
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• 2005

Embryonic stem(ES) cells have tremendous potential as a cell source for cell-based therapies. Realization of that potential will depend on our ability to understand and manipulate the factors that influence cell fate decision and to develop methods for getting enough cell numbers for clinical applications. Hematopoiesis has been widely studied, and hematopoietic differentiation from ES cells is a good model to study lineage commitment. In this study, we investigated stemness and compared the efficiency of hematopoietic differentiation using two different mouse embryonic stem cell lines TC-1 and B6-1. Although the two cell lines showed known stem cell properties with minor differences, the embryoid body formation efficiency in methylcellulose was much higher in TC-1 than B6-1. When measured potentials of hematopoietic differentiation using functional(colony-forming cell) and phenotypic(specific marker expression) assays, we found that TC-1 can differentiate into hematopoietic cells in methylcellulose culture but B6-1 cannot. These results imply that we can improve the efficiency of hematopoietic cell differentiation by selection of proper cell lines and this may be also applied in the differentiation of human embryonic stem cells.

• Archives of design research
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• v.11 no.3
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• pp.101-110
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• 1998

It is impossible that the corporation can make up good corporate images with sales promoting and pursuing profits only. To form impressive oorporate images, the corporation should acquire efficient marketing images and social oonfidences with the investment for the culture, the art, the preservation of public health, educations and social welfare programs. The oorporate image is indined to be formed by audiences subjectively even though the corporation makes great efforts to have its unique images. The audience has a tendency to have their subjective opinions about the corporate image based on several informations communicated by the corporation and various sources. The sensitive approach and emotional persuation can be efficient for making good corporate images because corporate images of audiences arise from emotional and sensitive factors rather than rational ones. There are many previous studies verifying that the humor effect should be one of powerful means for inducing concentrations, memorizations and persuations. The majority of popular advertisings are humor touched one and the questionaire proves that almost all university students participating sample test, prefer humorous advertisings to serious advertisings. The questionaire verifies that it is a most efficient method is using humor effects at product advertising to improve the corporate image indirectly. And there are very possitive reactions about applying humor effects for the corporate image advertising and the other advertisement types such as pamphlets and catalogues. The humor effect motivates the persuation with spiritual pleasures and intimacy, and it minimize the negative aspects of advertising for the formation of the corporate image. The humor effect is valuable not only for the formation of the corporate image reflecting the entity of the oorporation itself but also for the ideation of advertising communications. The study on the humor effect related with the corporate image is significant because the humor is a different version of the humanism based on deep oontemplation and oomprehension of human life rather than simple means to motivate laughers.

• Korean Journal of Cognitive Science
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• v.26 no.2
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• pp.167-207
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• 2015

This study focused on the awareness and coping methods of psychological trauma and human suffering in the contemporary era after the development of posttraumatic stress disorder(PTSD) including the situations in the Korean society, and proposed principles for an alternative paradigm of trauma recovery. Trauma is defined as an 'external' stress causing chronic suffering mediated by memory, and the American Psychiatric Association approved PTSD in the Diagnostic and Statistical Manual of Mental Disorders in 1980. The development of PTSD empowered moral legitimacy to the victims, opened a successful way to treatment, and accomplished explosive amount of research in the area of neurobiology and cognitive neuroscience. However, this also narrowed the understanding of human suffering, and the importance of an alternative coping method which overcomes the limitations of technical intervention became overlooked. Moreover, the Korean society has an underlying mechanism of replacing the matter of trauma to a problem of an individual. This is shown among the historical context of splitting and denial, and among medicalized bureaucracy. Trauma should be acknowledged as a social suffering, and searching for an alternative paradigm is in need. This study suggested the following principles; seeking for truth and justice, survivor as the agent of recovery emphasizing the responsibility of the community, ecological adaptations of recent bio-psychological achievements, and finally putting emphasis on continuous discussions about the definition of recovery.

• The Journal of the Korea Contents Association
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• v.7 no.4
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• pp.234-240
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• 2007

The purpose of this paper was to architecture optimal model of the scooped swing in high bar. The scooped swing was modeled to the double pendulum and was simulated with the Lagrange's equation of motion. Lagrange's method based on a energy approaching method was implemented as a equation of motion. The subject was a national man-gymnast(age 18yrs, height 153 cut mass 48 kg) and the high bar of SPIETH company was used to measure the scooped swing. Qualisys system(six MCU-240 cameras, QTM software)was used to capture data for imaging analysis. The solution of a model and data processing were solved in Mathematica5.0. The results were as follows: First model value of maximum bar displacement was longer than experimental value, that is, 0.02 m. Second, both angular pattern of segment1(HAT) had a increasing curve but curve patterns had a different concave and convex me. Third the experimental value of maximum angular angle of segment2(total leg) had larger than model value, that is, $4^{\circ}$. Conclusively, model parameters were quasi-optimized to obtain a quasi-match between simulated and actual performances. It hopes to simulate a human model by means of integrating musculoskeletal and neuromuscular system in the future study.

• The Korean Journal of Pesticide Science
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• v.20 no.1
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• pp.35-40
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• 2016

Recently, bacterial angular leaf spot disease, caused by Xanthomonas fragariae, causes severe damage in strawberry production and its' export to other countries, since the pathogen has been classified as an A2 quarantine pathogen. Typical the Angular Leaf Spot (ALS) disease represent that water-soaked angular spots symptoms, bacteria ooze exudate under relatively high humidity condition and later the spot become reddish brown on the leaf surface. The pathogen disseminated by irrigation water, infected mother plant and farmer's hand. In this study, we reported that rubbing inoculation method showed more effective in the pathogen dissemination than infiltration with needles, regardless the strawberry cultivars. Additionally, Dichloroisocyanurate (NaDCC) treatment in commercial strawberry fields provided reliable efficiency to reduce the bacterial angular leaf spot disease incidency and severity.

• The Journal of the Korea Contents Association
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• v.20 no.5
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• pp.457-466
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• 2020

The purpose of this study is to find out what the main agenda of social formation is and how it changes through the media by utilizing the news big data of COVID-19 which is spreading recently, and to suggest the direction of future reporting. In order to achieve the purpose of the research, 47,816 cases of news big data reported from December 31, 2019 to March 11, 2020 were divided into four periods based on the fourth stage of the crisis warning for infectious diseases, and a total of 20 topics were derived. Based on the results of the Topic Modeling analysis, this study proposed the following. First, it is necessary to refrain from provocative expressions such as "anxiety" and "fear" and use neutral and objective reporting terms. Second, more in-depth and contextual news production is required, breaking away from simple event news production. Third, it is necessary to prepare detailed crisis communication manuals for each situation related to infectious diseases. Fourth, we need reports that focus on citizens-led efforts to overcome the crisis. This research has the academic significance that it is the first paper to analyze news big data on COVID-19 using the Topic Modeling Analysis method, and the policy significance that can be used as the basis for developing national crisis communication policy.

• Journal of KIISE:Computer Systems and Theory
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• v.31 no.9
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• pp.514-526
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• 2004

Microarray is a new technique for gene expression experiment, which has gained biologist's attention for recent years. This technology enables us to obtain hundreds and thousands of expression of gene or genotype at once using microarray Since it requires manual work to analyze patterns of gene expression, we want to develop an effective and automated tools to analyze microarray image. However it is difficult to analyze DNA chip images automatically due to several problems such as the variation of spot position, the irregularity of spot shape and size, and sample contamination. Especially, one of the most difficult problems in microarray analysis is the block and spot addressing, which is performed by manual or semi automated work in all the commercial tools. In this paper we propose a new algorithm to address the position of spot and block using a new concept of regular structure grid searching. In our algorithm, first we construct maximal I-regular sequences from the set of input points. Secondly we calculate the rotational angle and unit distance. Finally, we construct I-regularity graph by allowing pseudo points and then we compute the spot/block address using this graph. Experiment results showed that our algorithm is highly robust and reliable. Supplement information is available on http://jade.cs.pusan.ac.kr/~autogrid.

• The Journal of the Korea Contents Association
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• v.10 no.10
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• pp.440-450
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• 2010

This study was an analysis of sport psychological application for the kinesthetic gifted children's selection and upbringing in Pusan University's center for kinesthetic gifted children from 2009.7 to 2010.2. The 60(athletics: 40, swimming: 10, gymnastics: 10) of kinesthetic gifted children were selected among the first, second and third year students from Pusan, Ulsan and Kyungsang-namdo without distinction of sex. We progressed summer and winter camp during vacation, and managed a special training program according to exercise items on every Saturday. We attempted experimental a field application, and obtained the following implications. First, the first and second year students were possible to test psychological measurement with supplementary explanation, and in the case of third grade, it was enough possible without any supplementary explanation. Second, multi-intelligence test was efficient as the method to check kinesthetic gifted children's intelligence and useful as the basic data for counseling. Third, the character types of kinesthetic gifted children were appeared preferring outgoing, intuition and emotions. Forth, with the FAIR concentration, we confirmed that the center's program effected positively on improving concentration. Fifth, we found the potential that the physical task commitment questionnaire and the exercise activity self-administer questionnair would be used as official psychological measurement tool after the review process of additional validity and reliability.

• Journal of Navigation and Port Research
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• v.28 no.10 s.96
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• pp.947-953
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• 2004

As a large scale port development in coastal waters proceeds step by step and populations in the vicinity of port are getting increased, the issue on 'how to dispose the treated municipal water and wastewater in harbor' brings peoples' concern. The submarine outfall system discharges the primary or secondary treated effluent at the coastline or in deep water, or between these two. The effluent, which has a density similar to that of fresh water, rises to the sea surface forming plume or jet, together with entraining the surrounding sea water and becomes very dilute. We intended in this paper to investigate the impact on dilution of effluent and the behavior of flume under the conditions of the seasonal and spatial temperature variations, which have not been noticeable in designing effective marine outfall system. To predict and analyze the behaviour and dilution characteristics of plume not just with the effluent temperature, but also with the seasonal variation of temperature of surround water, CORMIX( Cornell Mixing Zone Expert System)-GI have been applied. The results should be used with caution in evaluation the mixing zone characteristics of discharged water. We hope to help for the effective operation of outfall system, probable outfall design, protection of water quality, and warm water discharges from a power plant, etc.

• Microbiology and Biotechnology Letters
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• v.34 no.4
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• pp.299-305
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• 2006

In this study, we have isolated and identified a proteolytic bacterium from conventional Cheongkukjang. We also characterized several bioactivity of Cheongkukjang, which was fermented by an isolated strain. One strain out of about $10^4$ strains obtained from Cheongkukjang showed relatively high proteolytic activity was selected and named as a Bacillus subtilis LSH805 strain. White soy-bean Cheongkukjang possessed less odor and more viscous substance than black soy-bean Cheongkukjang. Cheongkukjang showed fibrinolytic activity, and about 1,500 mg fibrin was degraded after 20 h incubation. Although nitric oxide (NO) assays of soy-bean and Cheongkukjang were almost the same, their activities were significantly higher than that of no treatment. Activity of water fraction of Cheongkukjang was somewhat higher than that of soy-bean. Fibrinolytic and NO assays of Cheongkukjang suggest that Cheongkukjang, which was fermented by an isolated strain may be a useful candidate for natural fibrinolytic and macrophage-stimulating agents.