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Analysis of Soil Microbes in Continuous Cropping Fields of Gastrodia elata (천마 연작지의 토양 미생물상 분석)

  • Chang Su Kim;Eun Suk Lee;Hyun Soo Jung;Jung Hyun Yoo;So Ra Choi;Young Eun Song;Sang Young Seo;Min Sil Ahn
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2023.04a
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    • pp.36-36
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    • 2023
  • 천마(天麻, Gastrodia elata Blume)는 난초과(蘭草科, Orchidaceae)에 속하는 식물로 잎과 뿌리가 없어 탄소동화능력이 없으며, 뽕나무버섯균과 공생하는 기생식물이다. 천마는 노지 재배에 따른 안정생산 문제가 지속적으로 발생하고 있다. 혹한, 폭우 등 기상환경에 따른 연차간 수량성 차가 673~1,175kg/10a로 크고, 연작에 따른 수량성이 연작 1회 시 29%, 연작 2회 시 68%가 감소하는 경향을 보이고 있다. 따라서, 본 연구는 천마의 연작에 따른 토양내 미생물상의 변화를 분석하기 위해 수행하였다. 본 실험은 초작지, 연작 1회지, 연작 2회지 및 자생지의 각각 3곳에 대한 토양을 Metagenome 분석법을 활용하여 미생물상을 분석하였다. metagenome 분석 결과, 초작지 3곳의 시료를 이용하여 확보한 총 read는 699,221개였으며, 이 중에서 Eukaryota로 분류되지 않은 read는 1,377개(0.2%), no hit, not assign된 read는 10,510개 (1.5%), Bacteria로 분류된 read는 342,916개(49.0%), Eukaryota로 분류된 read는 총 344,418개(49.3%)였다. 그리고 천마의 생육에 영향을 주는 Fusarium 속과 종은 3곳 포장에서 고르게 분포하고 있으며, 총 11,242 read로 전체의 3.3%를 차지하였다. 연작 1회지 3곳의 시료를 이용하여 확보한 총 read는 655,097개였으며, 이 중에서 Eukaryota로 분류되지 않은 read는 1,694개(0.3%), no hit, not assign된 read는 18,985개(2.9%), Bacteria로 분류된 read는 312,201개(47.7%), Eukaryota로 분류된 read는 총 322,217개 (49.2%)였다. 그리고 천마의 생육에 영향을 주는 Fusarium 속과 종은 3곳 포장에서 고르게 분포하고 있으며, 총 11,597 read로 전체의 3.6%를 차지하였다. 연작 2회지 3곳의 시료를 이용하여 확보한 총 read는 651,624개였으며, 이 중에서 Eukaryota로 분류되지 않은 read는 1,753개(0.3%), no hit, not assign된 read는 7,995개(1.2%), Bacteria로 분류된 read는 307,178개(47.1%), Eukaryota로 분류된 read는 총 334,698개(51.4%)였다. 그리고 천마의 생육에 영향을 주는 Fusarium 속과 종은 총 43,877 read로 전체의 13.1%를 차지하였다. 자생지 3곳의 시료를 이용하여 확보한 총 read는 731,719개였으며, 이 중에서 Eukaryota로 분류되지 않은 read는 2,828개(0.4%), no hit, not assign된 read는 585개(0.1%), Bacteria로 분류된 read는 356,690개(48.7%), Eukaryota로 분류된 read는 총 371,616개(50.8%)였다. 그리고 천마의 생육에 영향을 주는 Fusarium 속과 종은 MJH01 자생지에서만 전체의 0.2%(총 660 read)로 극히 일부가 분리되었다. 따라서, 천마의 생육에 영향을 미치는 Fusarium 속과 종은 초작지 3.3%, 연작 1회지 3.6%, 연작 2회지 13.1%, 자생지 0.2%로 나타났으며, 연작 2회 시 급격히 증가하는 것으로 판단되었다.

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Immunocytochemical mapping of serotonergic neurons in the centrqal nervous system in the larva of Lucilia illustris (연두금파리 유충의 중추신경계에서 세로토닌 면역반응성세포의 동정)

  • 김관선;이봉희김우갑
    • The Korean Journal of Zoology
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    • v.36 no.1
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    • pp.6-13
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    • 1993
  • 연두금파리 유충의 뇌와 복신경절에 분포하는 세로토닌 면역반응성 세포를 면역조직화학적 방법을 이용하여 동정하였다. 세로토인세포는 뇌에 28개, 제1식도하신경절의 첫째마디에 6개, 둘째마디에 10개 그리고 세째마디에 6개가 각각 존재하였다. 그리고 앞가슴신경절에 6개, 가운데가슴신경절에 4개 그리고 됫가슴신경절에 4개가 각각 위치하였다. 또한 복부신경절에서는 첫새 마디부터 일곱째 마디까지 각각 4개가 존재하였고 마지막마디인 여덟째마디에서는 단지 두개의 세포만이 관찰되었다. 결국 연두금파리 유충의 중추신경계에는 모두 94개의 세로토닌 면역반응성 세포들이 분포하였다. 이들 세포로부터 뻗어나온 축색들은 뉴로파일내에서 분지하거나 횡연합섬유를 이루었다.

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Recognition of Dog Breeds based on Deep Learning using a Random-Label and Web Image Mining (웹 이미지 마이닝과 랜덤 레이블을 이용한 딥러닝 기반 개 품종 인식)

  • Kang, Min-Seok;Hong, Kwang-Seok
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2018.10a
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    • pp.201-202
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    • 2018
  • In this paper, a dog breed image provided by Dataset of existing ImageNet and Oxford-IIIT Pet Image is combined with a dog breed image obtained through data mining on Internet and a random-label is added. this paper introduces to recognize 122 classes of dog breeds and 1 class that is not dog breeds. The recognition rate of dog breeds using both conventional DB and collection DB was improved 1.5% over Top-1 compared to recognition rate of dog breeds using only existing DB. The image recognition rate about non-dog image, was 93% recognition rate in case of 10000 random DBs.

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A Study on Intrusion Detection in Network Intrusion Detection System using SVM (SVM을 이용한 네트워크 기반 침입탐지 시스템에서 새로운 침입탐지에 관한 연구)

  • YANG, Eun-mok;Seo, Chang-Ho
    • Journal of Digital Convergence
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    • v.16 no.5
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    • pp.399-406
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    • 2018
  • Much research has been done using the KDDCup99 data set to study intrusion detection using artificial intelligence. Previous studies have shown that the performance of the SMO (SVM) algorithm is superior. However, intrusion detection studies of new intrusion types not used in training are insufficient. In this paper, a model was created using the instances of weka's SMO and KDDCup99 training data set, kddcup.data.gz. We tested existing instances(292,300) of the corrected.gz file and new intrusions(18,729). In general, intrusion labels not used in training are not tested, so new intrusion labels were changed to normal. Of the 18,729 new intrusions, 1,827 were classified as intrusions. 1,827 instances classified as new intrusions are buffer_overflow. Three, neptune. 392, portsweep. 164, ipsweep. 9, back. 511, imap. 1, satan. Dogs, 645, nmap. 102.

Identification of Developmental Related Genes in the Lab Animal (실험동물 발생 유전자의 확인)

  • Lee, Jae-Dal
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.6
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    • pp.1407-1413
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    • 2009
  • The present study was conducted to gain insights into oocyte maturation and early embryo development, SELDI-TOF-MS was used to find the protein candidates that are specifically or prominently expressed in mouse oocytes at the in vitro matured metaphase II (MIl) and germinal vesicle (GV) stages. By using selected CM10 chip, found 16 candidates which were up-regulated in GV stage oocytes compared with in MIl stage oocytes, molecular weight are 8180 (2 candidates), 10226 (5 candidates), 15767 (5 candidates) and 16770 (4 candidates) Da respectively. And 29 candidates were higher in MIl than in GV stage oocytes, molecular weight are 10832 (3 candidates), 17744(8 candidates), 20122 (3 candidates), 22131 (3 candidates), 24857 (7 candidates) and 33507 (5 candidates) Da, respectively. All (45) candidate (0.2 and 1.0 % error tolerances) were performed real time RT-PCR analysis and further selected 13 more potential candidates.

Volatile Compounds Isolated from Edible Korean Fatsia Shoots (Aralia elata Seem.) (두릅의 휘발성 향기성분에 관한 연구)

  • Kim, So-Mi;Chung, Tae-Young
    • Applied Biological Chemistry
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    • v.39 no.5
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    • pp.389-397
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    • 1996
  • The volatile concentrate obtained from the edible Korean dureup plant (Aralia elata Seem.) by a distillation-extraction system was separated into hydrocarbon and oxygen-containing fractions, and the latter was further separated into nine subfractions by silica gel column chromatography. Gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) were utilized to identify 167 volatile compounds in the fractions. The volatile compounds included 72 hydrocarbons, 31 alcohols, 23 aldehydes, 16 esters, 10 acids, 6 ketons, 3 furans, 2 phenols, 1 indole, 1 oxide, 1 sulfide, and 1 lactone. ${\beta}-Caryopyllene$, a sesquiterpene hydrocarbon, was the most abundant volatile compound identified in Korean dureup (19.53%). Dureup oil was found to possess a woody or herbaceous aroma following sensory evaluation of each fraction and individual volatile component using a GC-sniff apparatus.

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A Study on the Relative Efficiency and Productivity Change of IT Firms received Policy Fund (정책자금 받은 IT 기업의 상대적 효율성 및 생산성 변화 연구)

  • Kang, Ho-Jung;Jeon, In-Soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.3
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    • pp.677-683
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    • 2009
  • This study measures the relative efficiency and productivity change of IT firms received policy fund using DEA model and Malmquist Index for 2006-2007. The main results of this study can by summarized as follows. First, in case of efficiency of CCR for 2006-2007, the number of efficient firms(CCR value is one) are six firms, six firms, respectively. Second, in case of efficiency of BCC for 2006-2007, the number of efficient firms(BCC value is one) are eleven firms, thirteen firms respectively. Third, In case of return to scale for 2006-2007, DRS are fifteen firms and fourteen firms respectively. IRS are two firms and one firm respectively. DRS firms can improve efficiency by the reduction of scale and IRS Firms can improve efficiency by the increase of scale. Fourth, Mean value of Malmquist Index representing productivity change for 2006-2007 are bigger than 1.00. This imply that productivity increase was achieved.

Analysis of Characteristics of the Cancelled Districts of Housing Redevelopment Project - Focusing on Decision Tree Analysis - (재정비사업 해제구역 의사결정 특성 연구 - 의사결정나무기법 중심으로 -)

  • Lee, Do-Ghil
    • Journal of the Korean Regional Science Association
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    • v.37 no.4
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    • pp.49-59
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    • 2021
  • This study aims to identify the characteristics of the cancelled districts of housing redevelopment and housing reconstruction project. The subject of this study is 189 project districts(121 promoted districts, 68 cancelled districts). Both 121 promoted districts and 68 cancelled districts were analyzed by Decision Tree Analysis. The first separation of the release zone influencing factors was made by the Development Actors. In other words, the most important independent variable for determining the release zone influence factor was shown to be the presence or absence of propulsion actors. Of the 89 districts without propellers, 41 were lifted and 48 were promoted, and 9 out of 100 districts with propellers were lifted and 91 were promoted. The second separation of the impact factors on the zone was then made by Land Owners, and the probability of cancellation increased if the number of landowners was less than 468 and 37 out of 62 were removed. On the other hand, four out of 27 districts with more than 468 landowners were lifted and 23 districts were promoted. The third separation was made by the Average Land Assessment, and 35 zones were lifted below the standard of KRW 269.64 million/m2 approximately KRW 8.91 million per pyeong, and two zones were lifted at higher official prices. In the second division, the number of landowners was 468 or more, and in node4, four areas were removed from areas with a public land area ratio of 29.43% or more, and no areas less were released. This study used SPSS Statistics 26 S/W for analysis.

Comparison of Conchocelis Formation in the Oyster Shell of Neopyropia Yezoensis with Water Temperature Change (수온 변화에 따른 방사무늬김(Neopyropia yezoensis) 패각 사상체의 각포자 형성량 비교)

  • Eun Taek Lee;Dal Sang Jeong;Chul Won Kim;Sung Je Choi
    • Journal of Practical Agriculture & Fisheries Research
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    • v.25 no.3
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    • pp.19-29
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    • 2023
  • This study investigated effect of water temperature change on the formation and release of conchospores of Neopyropia yezoensis. We observed that conchocelis growth and conchospores formation in oyster shell at labolatory during 7 weeks. In order to investigate the amount of conchospore formation in oyster shells, which was being cultured at 28℃, was moved to 10℃, 18℃, 28℃, and culture during 6 weeks. At 10℃, we observed an average of 127 for 1 week, 127 for 2 weeks, 95 for 3 weeks, 90 for 4 weeks, 76 for 5 weeks, and 75 for 6 weeks. At 18℃, we observed an average of 141 for 1 week, 135 for 2 weeks, 94 for 3 weeks, 153 for 4 weeks, 162 for 5 weeks, and 2 for 6 weeks. At 28℃, we observed an average of 167 for 1 week, 102 for 2 weeks, 148 for 3 weeks, 157 for 4 weeks, 270 for 5 weeks, and 138 for 6 weeks. Conchospores released from the shell grew into a young thalli in the culture for 6 weeks, and the number of ones was counted. The number of young thalli were investigated at 10℃, 0 for 1 week, 189 for 2 weeks, 200 for 3 weeks, 89 for 4 weeks, 56 for 5 weeks and 27 for 6 weeks. At 18℃, It observed 0 for 1 week, 26 for 2 weeks, 546 for 3 weeks, 16 for 4 weeks, 17 for 5 weeks and 154 for 6 weeks. It was not observed at 28℃.

Changes in Nutrient Contents of Children's Favorite Foods after Policy Implementation on Energy-Dense and Nutrient-Poor Foods in Korea (고열량·저영양 식품의 관리정책 시행에 따른 어린이 기호식품의 영양성분 변화)

  • Kim, Hyungjun;Lee, Youngmi;Yoon, Jihyun;Kim, So-young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.4
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    • pp.501-512
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    • 2017
  • This study analyzed changes in nutrient contents of children's favorite foods between March 2010 and July 2012 after policy implementation on energy-dense and nutrient-poor (EDNP) foods based on Special Act on Safety Control of Children's Dietary Life in Korea. A total of 6,684 products in the list of children's favorite foods by the Ministry of Food and Drug Safety in March 2010 and 7,347 products in July 2012 were classified into the following three types: food products (1) manufactured or sold in 2012 as well as 2010 (n=6,174), (2) discontinued in 2012 (n=510), and (3) newly introduced in 2012 (n=1,173). Among children's favorite foods manufactured or sold in 2012 as well as 2010 (n=6,174), calories and key nutrients in sweetened ices, breads, ice creams, deep-fried noodles/noodles, ready-to-eat (RTE) foods, and pizzas were improved in comparison to those in the other food groups. However, most of the changes in calories or key nutrient contents per 100 g (or 100 mL) fell below 1 kcal or 1 g. The newly introduced candies, breads, and carbonated beverages showed slightly greater improvements in calories and key nutrient contents per 100 g (or 100 mL) than the discontinued ones in 2012. On the other hand, some negative changes were found in newly introduced chocolates and RTE foods in comparison to the discontinued ones. Overall, policy implementation on EDNP foods seemed to induce changes in nutrient contents of children's favorite foods, yet the extent of the changes was limited.