• Journal of Life Science
• /
• v.25 no.2
• /
• pp.136-150
• /
• 2015

Pseudomonas syringae pathovar tabaci is a plant pathogenic bacterium that causes wildfire disease in tobacco plants. In P. syringae pv. tabaci, PsyI, a LuxI-type protein, acts as an AHL synthase, while primary and secondary sequence analysis of PsyR has revealed that it is a homolog of the LuxR-type transcriptional regulator that responds to AHL molecules. In this study, using phenotypic and genetic analyses in P. syringae pv. tabaci, we show the effect of PsyR protein as a quorum-sensing (QS) transcriptional regulator. Regulatory effects of PsyR on swarming motility and production of siderophores, tabtoxin, and N-acyl homoserine lactones were examined via phenotypic assays, and confirmed by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Further qRT-PCR showed that PsyR regulates expression of these virulence genes in response to environmental signals. However, an upstream region of the gene was not bound with purified MBP-PsyR protein; rather, PsyR was only able to shift the upstream region of psyI. These results suggested that PsyR may be indirectly controlled via intermediate-regulatory systems and that auto-regulation by PsyR does not occur.

• International Commerce and Information Review
• /
• v.17 no.2
• /
• pp.201-223
• /
• 2015

Concerning post-verification which is after the application of FTA preferential tariffs, in cases such as Korea-EFTA and Korea-ASEAN, the growing trend of post-verifications was restricted and there was no active research concerning this; whereas with Korea-EU FTA which adopted indirect verification for post-verification, the demand for post-verification has been rising constantly each year; and for Korea-US FTA which has adopted direct verification, a new approach to post-verification research is needed as it began genuine post-verification regarding many national enterprises only after a year and a half since the agreement. This study will consider the counter measures that can be taken for post-verification, through case of studies on the factors influencing violation of determining the country of origin for export company regarding verification of FTA. Result of this case study regarding the determining a violation of origin, The main cause of the violation factors on the essential & general principles(goods wholly obtained, value added criteria) are lack of understanding agreement & manpower. but Violation of the parties to a transaction & the origin certification are lack of advanced preparation and effected violation of principle of good faith. Finally, In this study help countermeasures of export company through the Detailed analysis of the type & implications deriving from verification of origin.

• Journal of the Korean Society for Precision Engineering
• /
• v.29 no.11
• /
• pp.1256-1263
• /
• 2012

The present study covers the ultrasonic patterning process to replicate micro-patterns on a polymer substrate. The ultrasonic patterning process uses ultrasonic waves to generate frictional heat between an ultrasonic horn and the polymer substrate, from which the surface region of the polymer substrate is softened sufficiently for the replication of micro-patterns. The ultrasonic patterning process can divided into two categories according to the direction of vibration transmission: direct patterning and indirect patterning. The direct patterning uses a patterned horn, and the ultrasonic vibration is transferred directly from the patterned horn to the substrate. On the contrary, the indirect patterning process uses a plain horn, and the micro-patterns are engraved on a mold that is located below the substrate. Thus, the micro-patterns are replicated as an indirect manner. In this study, these direct and indirect patterning processes are compared in terms of the replication characteristics. Additionally, the possibility of double-side patterning is also discussed in comparison with the conventional single-side patterning process.

• The Journal of Information Systems
• /
• v.17 no.1
• /
• pp.113-130
• /
• 2008

현대의 기업조직들은 역동적 경영환경변화에 직면하고 있다. 이러한 변화에 적응하기 위한 수단으로서, 많은 기업들이 정보기술의 도입을 적극적으로 고려하고 있다. 1990년대 중반에 등장한 ERP(Enterprise Resource Planning: 전사적 자원관리)는 이러한 정보기술의 대표적인 것으로 통합된 데이터베이스에 기초하여 기업의 연계된 업무기능을 지원하는 시스템이다. ERP시스템은 효용 및 효과 대비면에서 많은 장점을 가지고 있기 때문에 현재 우리나라의 많은 기업들이 ERP시스템을 도입하고 있다. ERP에 대한 기존의 연구들은 주로 ERP 도입 성공요소에 관한 내용이 주류를 이룬다. 그러나 이러한 주요성공요소들은 주로 시스템 개발과정의 특성만을 포함하곤 주요성공요소를 몇 개의 범주로 묶어 제시하는데 그 초점을 두고 있기 때문에, 이들 주요성공요소 간에 존재하는 상호영향관계를 충분히 반영하지 못하고 있는 실정이다. 이에 본 연구의 목적은 ERP CSF(Critical Success Factor : 주요성공요소)간에 존재하는 상호영향관계, 그리고 그러한 영향관계가 전체 ERP 성과에 어떤 효과를 가져다 주는가에 대한 총체적인 상호작용에 관한 연구가 부족하기 때문에, 이를 분석하고자 했다. 이 연구의 결과는 다음과 같다. ERP 주요성공요소 간에 존재하는 복잡한 상호영향관계를 실증분석을 통하여 측정하여 이들 간에 상호영향관계 및 선행관계가 있다는 것을 밝혀내었다. 다만, 일반적으로 BPR로 인하여 조직이 많이 변화하면 변화에 대한 사용자의 적응은 떨어질 것으로 생각되지만, 본 연구에서는 이들의 관계는 없는 것으로 밝혀졌다. 조직이 얼마나 많이 변화하였는가보다는 변화관리가 사용자가 적응하는데 더 많이 영향을 미친다고 나타나고 있다. 결국 BPR의 정도 또한 변화관리를 통해서는 사용자의 적응에 간접적으로 유의한 영향을 미치는 것으로 밝혀졌다.

• Journal of KIISE:Databases
• /
• v.29 no.2
• /
• pp.99-109
• /
• 2002

ROLAP(Relational Online Analytical Processing) is a process and methodology for a multidimensional data analysis that is essential to extract desired data and to derive value-added information from an enterprise data warehouse. In order to speed up query processing, most ROLAP systems pre-compute summary tables. This process is called 'cube generation' and it mostly involves intensive table sorting stages. (1) showed that it is much faster to generate ROLAP summary tables indirectly using a MOLAP(multidimensional OLAP) cube generation algorithm. In this paper, we present such an indirect ROLAP cube generation algorithm that is fast and scalable. High memory utilization is achieved by slicing the input fact table along one or more dimensions before generating summary tables. High speed is achieved by producing summary tables from their smallest parents. We showed the efficiency of our algorithm through experiments.

• Journal of Life Science
• /
• v.7 no.4
• /
• pp.392-401
• /
• 1997

The poliovirus is a small, and non-enveloped virus. The RNA genome of poliovirus is continuous, linear, and has a single open reading frame. This polyprotein precursor is cleaved proteolytically to yield mature products. Most of the cleavages occur by viral protease. The mature proteins derived from the P1 polyprotein precursor are the structural components of the viral capsid. The initial cleavage by 2A protease is indirectly involved in the cleavage of a cellular protein p220, a subunit of the eukaryotic translation initiation factor 4F. This cleavage leads to the shut-off of cap-dependent host cell translation, and allows poliovirus to utilize the host cell machinery exclusively for translation its own RNA, which is initiated by internal ribosome entry via a cap-independent mechanism. The functional role of the 2B, 2C and 2BC proteins are not much known. 2B, 2C, 2BC and 3CD proteins are involved in the replication complex of virus induced vesicles. All newly synthesized viral RNAs are linked with VPg. VPg is a 22 amino acid polypeptide which is derived from 3AB. The 3C and 3CD are protease and process most of the cleavage sites of the polyprotein precursor. The 3C protein is also involved in inhibition of RNA polymerase II and III mediated transcription by converting host transcription factor to an inactive form. The 3D is the RNA dependent RNA polymerase. It is known that poliovirus replication follows the general pattern of positive strand RNA virus. Plus strand RNA is transcribed into complementary minus strand RNA that, in turn, is transcribed for the synthesis of plus strand RNA is transcribed into complementary minus strand RNA that, in turn, is transcribed for the synthesis of plus strand RNA strands. Poliovirus RNA synthesis occurs in a membranous environment but how the template RNA and proteins required for RNA replication assemble in the membrane is not much known. The RNA requirements for the encapsidation of the poliovirus genome (packaging signal) are totally unknown. The poliovirus infection cycle lasts approximately 6 hours.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
• /
• v.3 no.3
• /
• pp.159-165
• /
• 2005

The characterization of radiological contamination inside pipes generated during the decommission of a nuclear facility is necessary before pipes can be recycled or disposed. But, existing direct measurements of radioactive contamination level using the survey-meter can not estimate the characteristic of contamination on a local area such as the pipe inside. Moreover, the measurement of surface contamination level using the indirect methods has many problems of an application because of the difficulty of collecting sample and contamination possibility of a worker when collecting sample. In this work, plastic scintillator was simulated by using Monte Carlo simulation method for detection of beta radiation emitted from internal surfaces of small diameter pipe. Simulation results predicted the optimum thickness and geometry of plastic scintillator at which energy absorption for beta radiation was maximized. In addition, the problem of scintillator processing and transferring the detector into the pipe inside was considered when fabricating the plastic detector on the basis of simulation results. The characteristic of detector fabricated was also estimated. As a result, it was confirmed that detector capability was suitable for the measurement of contamination level. Also, the development of a detector for estimating the radiological characteristic of contamination on a local area such as the pipe inside was proven to be feasible.

• Clinical and Experimental Reproductive Medicine
• /
• v.36 no.2
• /
• pp.89-100
• /
• 2009

SLE 등의 자가면역질환 병인론에 있어서 $CD4^+$ T 조력 림프구 (T helper cell)의 기능과 항상성 조절의 이상이 중요하다는 것은 알려진 사실이지만 $CD4^+$ T 조력 림프구 (T helper cell)의 발달과 기능에 대한 성호르몬의 정확한 역할에 대하여는 아직 확립되어 있지 않은 실정이다. 에스트로겐의 $CD4^+$ T 림프구에 대한 여러 방면의 작용에 대한 결과는 에스트로겐 매개 신호가 전달되는 정황에 매우 의존적이라고 할 수 있으며, 또한 에스트로겐이 B 림프구와 항원발현세포들 같이 T 림프구 기능을 변화시킬 수 있는 다른 분획 세포들의 기능에 대해서도 영향을 미칠 수 있다는 사실을 고려한다면, 에스트로겐이 $CD4^+$ T 림프구매개반응에 미치는 작용은 매우 복잡하다고 할 수 있다. 현재로선 이런 과정들과 관련된 에스트로겐의 정확한 기전에 대한 연구 성과는 현재로선 아직걸음마 단계에 지나지 않는다고 볼 수 있다. 일부 분자학적 표적들의 발견에도 불구하고 ER-$\alpha$와 ER-$\beta$, 또는 이런 표적들의 조절에 대한 유전자적 또는 비유전자적 경로의 상대적 역할에 대해서는 현재로선 알려진 바가 많지 않은 것 또한 사실이며, T 림프구 자체의 미세 환경과 ER 매개 전사반응에 영향을 미칠 수 있는 방법 역시 아직까진 알려진 바가 없다. 따라서 에스트로겐의 $CD4^+$ T 림프구에 대한 다각적 영향을 규명하는 데 있어 이런 경로들에 대한 보다 체계적이고 상세한 연구가 반드시 필요하다. 최근 골다공증의 병인에 있어 에스트로겐에 의한 TNF 및 RANK-RANKL계 억제에 대해 연구가 심도 있게 진행됨으로써 결과적으로 다른 시토카인과 면역세포들에 대한 영향이 간접적으로 규명되고 있는 점은 매우 고무적인 현상이라고 여겨진다. 이러한 연구들은 추후 에스트로겐의 면역 및 염증체계에 대한 특징적 작용을 규명하는데 있어서 밑거름이 될 수 있을 것이다. 나아가 호르몬 자체, 또는 SERM 같은 준호르몬제제들이 면역계에 미치는 영향을 분자학적으로 규명함으로써 향후 이러한 지식들이 또한 류마티스성 자가면역질환이나 만성염증성질환, 또는 전신성 감염질환에 있어 면역글로불린이나 기타 다른 기존 치료 약제들을 대체 보완할 수 있는 호르몬 치료로 이어질 수 있는 결정적인 단서를 제공할 수 있을 것이라 기대할 수 있을 것이다.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.54 no.3
• /
• pp.299-306
• /
• 2009

In nitrogen-limited conditions, rhizobia lead to formation of nitrogen-fixing nodules on the roots of leguminous plants. The process of nodulation is autoregulated by pre-existing nodules in the same root system. The altered profile of sap proteins by inoculation with B. japonicum may indicate presence of a signal responsible for autoregulation transferred through stem. The 20 kDa protein enhanced by innoculation significantly decreased in intensity from 2.5 to 7 days after inoculation (DAI). However 6 kDa protein did increase during such a transition period. Western blot analysis showed that both 20 kDa and 6 kDa were cross-reacted with the SKTI antiserum. This suggests that SKTI may be involved in soybean nodulation by specific induction and degradation in stem sap during early stage of nodulation. RNAi technique and Agrobacterium rhizogenes-mediated transformation were applied to investigate the function of SKTI in nodulation. We have found that the number of rhizobium-induced nodule was much less in SKTIi-silenced hairy roots than the non-silenced. Indeed the quantitative RT-PCR showed that the expression level of SKTI gene was reduced over 40% in the transgenic hairy roots compared to the non-transgenic. It appears that the observed early induction of SKTI and degradation into small peptide in a specific time manner may be involved in autoregulation of nodulation in soybean and the specific mechanism of such regulation remains to be investigated.

• Plant Journal
• /
• v.11 no.1
• /
• pp.39-49
• /
• 2015

The objective of this study is to improve the classification structure of commodity code for piping material management which is considered as the fundamental of commodity code and piping material management system. It enhances the efficiency of piping material management directly or indirectly affecting the engineering, procurement and construction in a petrochemical plant projects. To establish an improved code classification structure, this study identifies the problems of former code classification structure in details, as well as the characteristics of other domestic and global EPC company's code classification structures and presents the improved direction considering the recently mega-sized and specialized projects. Accordingly, to efficiently enhance piping material management, the improved code classification structures have been derived from defining suitable code classification structure for specific piping component, adding more standard attribute, expanding the number of code digits and classifying code hierarchy. The results of applying the improved classification structure of commodity code to on-going project have led to reduce the rate of rework from 4.98% to 2.48% for developing purchase description and also have saved working time for executing piping design by 3D modeling from 6 months by two persons to 4 months by a person which is decreased 67% consequently. In addition, the structures of pyramid code management have resulted to accumulation and analysis of the various piping data for other disciplines such as procurement and estimation team which require commodity code information through the company's material control system.