• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.2
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• pp.179-184
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• 2021

Stratum corneum known as a skin barrier, which maintains water in skin, is the outer layer of the skin. Natural moisturizing factors (NMF) are one of the constituents in stratum corneum and amino acids are the highest components among NMF. In this study, we designed stearic acid-based solid lipid nanoparticles (SLNs) for improved skin penetration of serine (Ser). Ser-capsulated SLN was manufactured by double-melting emulsification method. The mean particle size and zeta potential of SLNs were 256.30 ~ 416.93 nm and -17.60 ~ -35.27 mV, respectively. The higher the degree of hydrophobicity or hydrophilicity of emulsifiers, the smaller the particle size and the higher the stability and capsulation rate. In addition, skin penetration was conducted using SkinEthic^TM RHE which is one of the reconstructed human epidermis models. The results of Ser penetration demonstrated that all SLNs enhanced than serine solution. The amount of enhanced Ser penetration from SLNs were approximately 4.1 ~ 6.2 times higher than that from Ser solution. Therefore, Ser-loaded SLN might be a promising drug delivery system for moisturizing formulation in cosmeceutical.

• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.486-494
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• 2021

Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) is sensitive to low temperature and shows retarded growth under 10℃. Although early transplanting guarantees higher returns, it requires cost and labor to maintain the appropriate temperature for plant growth. Therefore, cultivars tolerant to chilling stress is necessary to reduce the cost and labor requirements. The purpose of this study is to analyze data on plant growth and fruit enlargement under continuous chilling night temperature to develop new cultivars tolerant to chilling temperature. Two cultivars expected to have chilling tolerance and another cultivar sensitive to chilling temperature were grown in greenhouses with chilling and optimal night temperature conditions. In the early growth stage after transplanting, the cultivars expected to have chilling tolerance showed better vine length, fresh weight and dry weight. However, one of the tolerant cultivars showed significantly lower vine length, leaf length and width, and petiole length than the sensitive cultivar during pollination period and later growth stage, showing genotype specific responses. The fruit length, width, and weight were also significantly lower in the tolerant cultivar. The fruit set ratio was significantly higher in the chilling sensitive cultivar than the two tolerant cultivars. These results suggest that the present chilling tolerant cultivars in watermelon were selected based on their performance in the early growth stage, and further studies on chilling tolerance in different growth and development stages are required to develop cultivars adapted to various forcing cultivation systems.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.31-31
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• 2020

본 연구는 대표적인 봄 꽃 식물인 진달래(Rhododendron mucronulatum Turcz.)의 개화시기를 예측하기 위해 지난 9년간(2011년-2019년) 주왕산 지역에 생육하는 진달래의 식물계절자료(파열·개화·개엽·만개·낙엽)와 기상자료(일평균기온·일최고기온·일최저기온)를 토대로 이탈리아 생물기상연구소(IBMET)의 Chill Day 개화 예측모형인 생물계절모형을 실시하였다. 생물계절모형에 의한 예상 발아일간 편차의 제곱을 최소로 하는 조합은 기준온도 5℃, 저온요구량과 가온요구량은 97.94로 나타났다. 즉, 휴면해제일로부터 기준온도 5℃로 Chill Day를 누적시켜 97.94에 도달하는 날짜가 낙엽~내생휴면해제일이자 내생휴면해제일~발아기간까지의 값이며, 내생휴면해제일을 기점으로 개화일까지 102.93이 개화에 필요한 가온량으로 나타났다. 2011년부터 2019년까지 개화예상일을 기상청 회귀모형을 실관측기온에 적용한 결과 오차는 MAE=1.44이며, 생물계절모형을 적용할 경우 오차는 MAE=1.39, 기준온도 5℃일 경우 MAE=4.23, 기준온도 6℃일 경우 MAE=5.47, 기준온도 7℃일 경우 MAE=5.05로 나타나 생물계절에 의한 관측과 기상청의 회귀모형이 가장 유사한 것으로 나타났다. 가장 최근인 2018년과 2019년의 기상청 회귀모형와 생물계절모형의 개화 예측일을 비교한 결과, 2018년의 경우 청송지역의 진달래는 기상청 회귀모형에서 3월 30일 전후로 개화를 예상하였고 생물계절모형은 기준온도 5℃에 적용할 경우 내생휴면일에 가장 근접한 날은 3월 26일이였으며 이를 기준으로 가온량의 합이 102.93에 가깝게 되는 날인 4월 2일을 전후로 개화를 예측하였다. 실제 청송 주왕산의 진달래는 4월 3일에 개화를 시작하여 생물계절모형과 매우 유사함을 확인하였다. 2019년의 경우 청송지역의 진달래는 기상청 회귀모형에서 3월 25일 전후로 개화를 예상하였고 생물계절모형은 기준온도 5℃에 적용할 경우 내생휴면일에 가장 근접한 날은 3월 8일이였으며 이를 기준으로 가온량의 합이 102.93에 가깝게 되는 날인 3월 29일을 전후로 개화를 예측하였다. 실제 청송 주왕산의 진달래는 4월 5일에 개화를 시작하여 오히려 생물계절모형과 더욱 유사함을 확인하였다.

• Journal of Venture Innovation
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• v.6 no.3
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• pp.241-263
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• 2023

Recently, the aging of SME managers has emerged as a major issue in business succession. The smooth succession of small and medium-sized enterprises (SMEs), which are the industrial base of Korea, is becoming an important issue for enhancing the vitality of the national economy, such as job creation, beyond mere discussion of inheritance. At this point, this study conducted an empirical analysis on the factors affecting corporate succession intention. As independent variables, corporate Capability consisting of employee competency, marketing competency, and digital competency, human factors such as managers, successors, and third-party stakeholders, and institutional factors such as legislation, taxation, and trust were set. In addition, the effect of adjusting the sales scale was verified. To this end, a survey was conducted targeting representatives who are currently running small and medium-sized enterprises (SMEs). The total number of valid questionnaires was 315. Based on this, an empirical analysis was conducted using the hierarchical regression method. The results of the empirical analysis are as follows. First, marketing competency and digital competency, managerial competency and successor competency, taxation and trust had a significant positive (+) effect on corporate succession intention. However, a significant influence relationship between the third stakeholder, employee competency, and corporate succession intention was not tested. Among the factors that have a significant impact, the size of the influence was in the order of taxation, successor competency, executive competency, trust, marketing competency, and digital competency. Second, the size of sales was found to moderate the relationship between the variables that had a significant effect on corporate succession intention, that is, management competency, successor competency, marketing competency, digital competency, taxation and trust, and corporate succession intention. Based on these research results, academic, practical, and institutional implications for smooth business succession are presented.

• Journal of Venture Innovation
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• v.6 no.1
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• pp.139-159
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• 2023

As the founders of small and medium-sized businesses age, the issue of business succession becomes increasingly important. In this study, we empirically analyzed the impact of various human and institutional factors on corporate succession intentions, including the CEO's competency, the successor's competency, third-party stakeholders, and institutional factors such as legislation, taxation, and trust. We surveyed CEOs who were currently running small and medium-sized enterprises (SMEs), and received a total of 201 valid questionnaires. We then conducted an empirical analysis using the multiple regression analysis method. Our results showed that both the CEO's competency and the successor's competency, which are human factors, had a significant positive impact on corporate succession intention. Institutional factors such as taxation and trust also had a significant positive impact on corporate succession intention. However, third-party stakeholders and legislation did not have a significant effect on corporate succession intention. Among the factors that had a significant impact, the size of the influence was in the order of successor's competency, CEO's competency, taxation, and trust. These research results have both academic and practical implications. By going beyond existing studies that focus solely on human factors, we have revealed that institutional factors such as taxation and trust have a very important impact on corporate succession.

• Journal of fish pathology
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• v.4 no.1
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• pp.1-11
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• 1991

Histopathological changes and methemoglobinemia of the eels (Anguilla japonica) reared at six culture farms in the vicinity of Y$\check{o}$su city were investigated under the conditions of $22.0{\sim}29^{\circ}C$, 4.33~7.33ppm of D.O., pH 6.1~8.2 and 0.03~10.1ppm of nitrite from December, 1989 to June, 1990. All of the eels showed no any abnormal state in action, color and blood, but the eels reared in pH 6.0 had planty of mucus and stickiness on their body surface. Methemoglobin levels were 1.03~9.86% of total erythrocyes in Dec. and 5.05~25.69% in June, and poikilocyte levels were 5.8~30.17% in Dec. and 8.67~74.45% in June. These results indicate the fact that the water management of the culture farms was worse in summer than in winter, even though not good in winter. Hypertrophy was found in the gill, hepatic and kidney tissue of the eels in all the culture farms. In March and June, Fish of all culture farms appeared the detachment of gill lamella epithelium cells, pyknosis and sinusoids of nucleus in the hepatic tissue and hemosiderosis in the kidney tissue. The trend of increase in methemoglobin levels appeared in the fish of culture farms with high nitrite concentration, which didn't appear equally in all the culture farms. However, in the culture farms of pH 6.0, methemoglobin and poikilocyte levels were increased independently of concentration of nitrite, and degeneration in the tissue of the gill, liver and kidney was also appeared.

• Korean Journal of Agricultural Science
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• v.13 no.1
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• pp.82-89
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• 1986

This experiment was carried out to determine the optimum freezing and thawing rates of the hamster embryos. The female hamsters were induced to superovulate by intraperitoneal injections of 30 i.u. PMSG and mated with males of the same strain of 4 days the PMSG injection. They were killed and embryos were flushed from the oviduct and uterine horn on 3 days after mating. Embryos were flushed with a modified Dulbecco's phosphate-buffered saline and equilibrated with 1.5 M-dimethylsulphoxide by a 3-step procedure. The freezing rates of the samples were $1^{\circ}C/min$ from room temperature to $-6^{\circ}C$ and the samples were seeded at $-6^{\circ}C$. After being held for 3 min at the seeding temperature, the rates were $0.3^{\circ}C/min$ from $-6^{\circ}C$ to $-35^{\circ}C$. From $-35^{\circ}C$ to $-70^{\circ}C$, the rates were divided into $0.1^{\circ}C/min$, $1^{\circ}C/min$ and $10^{\circ}C/min$, respectively. At $-70^{\circ}C$ the samples were plunged directly into liquid nitrogen. The samples were thawed at $4^{\circ}C/min$ and $12^{\circ}C/min$ from $-196^{\circ}C$ to $37^{\circ}C$, and for 2 min in $37^{\circ}C$ water bath, respectively. The average numbers of ovulation points and embryos recovered were 35.1 and 27.0 appearing 77.0% recovery rates. Eight cell embryos in the embryos recovered were 24.8. The survival rates of embryos according to the freezing rates were 55.5~67.7% at $0.1^{\circ}C/min$, 58.8~64.9% at $1^{\circ}C/min$ and 40.5~44.7% at $10^{\circ}C/min$, respectively. The survival rates at $10^{\circ}C/min$ were significantly low. The survival rates of embryos according to the thawing rates were 53.5% at $4^{\circ}C/min$, 53.7% at $12^{\circ}C/min$ and 59.1% in $37^{\circ}C$ water bath. The survival rates, in $37^{\circ}C$ water bath were slightly higher, but we did not find any differences among them. In conclusion, the best freezing rates of hamster embryos were $1^{\circ}C/min$ from the room temperature to $-6^{\circ}C/min$, $0.3^{\circ}C/min$ from $-6^{\circ}C/min$ to $-35^{\circ}C$ and $-0.1^{\circ}C/min$ or $1^{\circ}C/min$ from $-35^{\circ}C$ to $-70^{\circ}C$. The hamster embryos thawed for 2 min in $37^{\circ}C$ water bath showed the best survival rates.

• KSBB Journal
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• v.23 no.4
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• pp.329-334
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• 2008

The antioxidant activity and the qualitative analysis of Acanthopanax sessiliflorum Seeman were studied by partially purified extract using various methods: extraction by using ethanol solutions and temperatures, and absorption to Diaion HP20 column chromatography using 10%, 20%, 40%, 60% ethanol solutions. Major constituents, chlorogenic acid, caffeic acid, eleutheroside E, was determinated by HPLC method in Acanthopanax sessiliflorum S. 10% and 20% ethanol solutions contain chlorogenic acid (3.020$\pm$0.080%, 20.500$\pm$1.150%, respectively). 40% ethanol solution contains caffeic acid and eleutheroside E (12.270$\pm$0.360%, 1.670$\pm$0.140%, respectively). Diaion HP20 fractions (10%, 20%, 40%, 60% ethanol solutions) showed the scavenging activity of radicals and reactive oxygen species with the $IC_{50}$ values of $81.534{\pm}0.992{\mu}g/ml$, $1.748{\pm}0.098{\mu}g/ml$, $11.487{\pm}1.768{\mu}g/ml$, $21.960{\pm}0.547{\mu}g/ml$ against 1,1-diphenyl-2-picrylhydrazly radical and $1713.548{\pm}34.565{\mu}g/ml$, $131.419{\pm}2.235{\mu}g/ml$, $200.681{\pm}2.444{\mu}g/ml$, $757.897{\pm}6.868{\mu}g/ml$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. Especially, 20% and 40% ethanol fractions showed more antioxidant activity than dl-$\alpha$-tocopherol. These results suggest that Acanthopanax sessiliflorum S. extract and Diaion HP20 fractions may be useful as a potential source of nutraceutical and cosmetic products.

• Korean Journal of Organic Agriculture
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• v.10 no.3
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• pp.87-99
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• 2002

The development of insecticidal soaps made by various fatty acids and organic control for insect by using of insecticidal soap(fatty acid salts) were employed. And the results were as follows: 1. To determine the input volume of potassium hydroxide for saponification of fatty acids, there were compared to individual acid value of fatty acids. in case of coconut fatty acids for saponification was 266.3 mg of potassium hydroxide(266 g/1kg of fatty acid) was calculated. 2. To make the 25% soap content by coconut fatty acids, there was required for the 266g of potassium hydroxide and 3,459 liter of soft water. Then the liquor of soap was 4,644 liter. 3. The progress of insecticidal soap made by fatty acids was accomplished indirect heating and stirring reactor (1)to make the volume of potassium hydroxide solution and warming up(90$^{\circ}$) (2)input volume of individual fatty acid (3)more than 30 min stirring reaction (4)cooling progress. 4. Insecticidal value of insecticidal soap was observed more than 92% the dilution of 50 dilution solution in consecutive 5 days of 2 treatments of the 25% soap made by coconut fatty acids on the red pepper. And insecticidal value of insecticidal soap was observed more than 94% the 100 dilution solution in consecutive 5 days of 3 treatments of the 25% soap made by coconut fatty acids on red pepper and cabbage. 5. The treatment of two times of 25% soap made by coconut fatty acids at the 50 dilution solution and 100 dilution solution in spider mite on red bean has 100% insecticidal value. 6. There was no observation phytotoxic sypmtons on red pepper in field, except for 25% soap made by carprylic acid. 7. Over the two times over 0.1% in addition of isopropyl alcohol was to improvement the insecticidal effect, but there was no effect in addition of diatomaceous earth.

• The Korean Journal of Malacology
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• v.32 no.3
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• pp.231-240
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• 2016

Techniques were developed for holding and conditioning of Pacific oysters, Crassostrea gigas, in a closed recirculating system. Experimental adults were used 500 oysters ( x two system, total 1,000 oysters) which were collected in $20^{th}$ March 2016 from long-line aquaculture farm at the south coast of Korea. During conditioning periods concentrated live microalgae as Isochrysis sp. $15{\times}10^7cells/mL$, Tetraselmis sp. $2{\times}10^7cells/mL$ and Pheaodactylum sp. $18{\times}10^7cells/mL$ were added 5 L every day, respectively which micro algae were functioned as diets and biological filter. Over all experimental periods total water exchange rate was 21.3% (daily 0.5%). Over 42 days conditioning, female and male oysters were maturated 90.9% and 94.4%, respectively. Survival rate was 98.7%. Mean shell hight (8.3 mm), total wet weight (19.2 g), meat wet weight (5.0 g) and shell wet weight (13.6 g) were significantly increased (P < 0.05). Water quality parameters including the water temperature ($22.1{\pm}0.4^{\circ}C$), salinity ($24.9{\pm}04$), dissolved oxygen (5.1-7.9 mg/L) and pH ($7.93{\pm}0.15$) were kept stable. Concentration of dissolved inorganic nutrient as ammonia (1.96-0.35 mg/L), nitrite (0.03-0.16 mg/L), nitrate (1.34-0.47 mg/L), DIP (0.42-0.03 mg/L) and silicate (3.83-0.00 mg/L) were significantly decreased throughout experiment except nitrite which was increased (P < 0.05), but nitrogenous components stayed below toxic levels (ammonia 0.0-5.5 mg/L, nitrite 0.0-460.0 mg/L) which indicated that closed recirculation system with microalgae based bio-filter could supply sufficiently environment condition to holding and conditioning of oyster.