• Title/Summary/Keyword: 가스멸균법

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Bioterrorism and Environmental Decontamination (생물테러와 환경소독)

  • Jeong, Woo-Dong;Mun, Sung-Min;Yoon, Je-Yong
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.9
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    • pp.1051-1059
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    • 2007
  • Bioterrorism intends to cause mass casualties and social panic by means of malicious pathogenic microorganisms. Environmental decontamination becomes very important as a follow-up measure if that happens. Conventional methods for decontamination is that aqueous disinfectants are being sprayed for killing or not spreading microorganisms with the purpose of preventing infection. However, these procedures are not enough to perfectly sterilize space or surface inside of building, requiring additional measures such as surface disinfection or gas treatment methods. This article deals with the issues about the present decontamination procedures, global trends, in order to formulate suggestion for advanced environmental decontamination.

Application of $CO_2$ laser in Minor Surgery of Oral Soft Tissue : Case Reports (연조직 소수술에서 $CO_2$ 레이저의 적용 증례)

  • Park, Ju-Hyun;Jeon, Young-Mi;Kwon, Jeong-Seung;Ahn, Hyung-Joon
    • Journal of Oral Medicine and Pain
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    • v.35 no.3
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    • pp.177-182
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    • 2010
  • Conventional surgical therapy for oral soft tissue includes the use of scalpel, diathermy, cryotherapy and electrosurgery. But, these therapies have some surgical problems. Nowadays, laser surgery can be considered as the another option for conventional surgical therapy. Compared to conventional surgical therapies, advantages of laser therapy include maintenance of sterile conditions, promotion of wound healing, reduction of bleeding, less instruments, post operative pain reduction, less scar, saving cost by using fewer materials, staff and time. Carbon dioxide ($CO_2$) laser uses gaseous medium, and has long wavelength about 10,600nm. The first advantage of $CO_2$ laser for surgical treatment of oral soft tissue is hemostasis and visibility improvement by making relatively dry field. These case reports are about cases of minor surgery of oral soft tissue using $CO_2$ laser, and emphasize advantages of laser compared to conventional surgical therapies.

THE EFFECT OF NAOCL TREATMENT AND STERILIZATION PROCEDURES ON THE CORROSION OF ENDODONTIC FILES (차아염소산나트륨 처리와 멸균법이 근관 치료용 파일의 부식에 미치는 영향에 관한 연구)

  • Yang, Won-Kyung;Ra, Yoon-Sik;Lee , Young-Kyoo;Son, Ho-Hyun;Kim, Mi-Ri
    • Restorative Dentistry and Endodontics
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    • v.30 no.2
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    • pp.121-127
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    • 2005
  • A variety files made of stainless steel (S-S) or nickel-titanium (Ni-Ti) are used during endodontic treatment. The purpose of tt)is study was to evaluate the corrosion susceptibility of S-S and Ni-Ti endodontic files. Three brands of files were used for this study: $K-flex^{(R)}$ S-S files (Maillefer, USA), $Profile^{(R)}$ Ni-Ti files (Maillefer, USA), $K-3^{(R)}$ Ni-Ti files (SybronEndo. USA). 120 files of each brands (21mm, ISO size $\#20$) were divided into 12 groups according to 1) sterilization methods using Autoclave or Ethylene Oxide (E-O) gas, 2) Irrigation solutions using $5.25\%$ NaOCl or Saline, 3) the number of sterilization (1, 5, 10 times), After above procedures, each of the files was inspected by three examiners with a light microscope and camera at X25. Each file was judged and ranked according to the following criteria: 0;, no corrosion, 1; mild corrosion, 2; moderate corrosion, and 3; severe corrosion. The files of high score were examined under the Scanning Electron Microscope. Data were statistically analyzed with the Kruskal-Wallis test (p < 0.05). Most of the ten time-autoclaved files had showed mild to moderate corrosion. But, one or five time-autoclaved files did not show corrosive surface. NaOCl treatment and E-O gas sterilization did not influence on corrosion. There was a significant difference in corrosion susceptibility between sterilization methods and the number of autoclaving. However, there was no significant difference between brands and file materials.

Improvement of Radiosynthesis Yield of [11C]acetate ([11C]아세트산의 방사화학적 수율 증가를 위한 연구)

  • Park, Jun Young;Son, Jeongmin
    • The Korean Journal of Nuclear Medicine Technology
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    • v.22 no.2
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    • pp.74-78
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    • 2018
  • Purpose $[^{11}C]$acetate has been proved useful in detecting the myocardial oxygen metabolism and various malignancies including prostate cancer, hepatocellular carcinoma, renal cell carcinoma and brain tumors. The purpose of study was to improve the radiosynthesis yield of $[^{11}C]$acetate on a automated radiosynthesis module. Materials and Methods $[^{11}C]$acetate was prepared by carboxylation of grignard reagent, methylmagnesium chloride, with $[^{11}C]$$CO_2$ gas, followed by hydrolysis with 1 mM acetic acid and purification using solid phase extraction cartridges. The effect of the reaction temperature ($0^{\circ}C$, $10^{\circ}C$, $-55^{\circ}C$) and cyclotron beam time (10 min, 15 min, 20 min, 25 min) on the radiosynthesis yield were investigated in the $[^{11}C]$acetate labeling reaction. Results The maximum radiosynthesis yield was obtained at $-10^{\circ}C$ of reaction temperature. The radioactivities of $[^{11}C]$acetate acquired at $-10^{\circ}C$ reaction temperature was 2.4 times higher than those of $[^{11}C]$acetate acquired at $-55^{\circ}C$. Radiosynthesis yield of $[^{11}C]$acetate increased with increasing cyclotron beam time. Conclusion This study shows that radiosynthesis yield of $[^{11}C]$acetate highly dependent on reaction temperature. The best radiosynthesis yield was obtained in reaction of grignard reagent with $[^{11}C]$$CO_2$ at $-10^{\circ}C$. This radiolabeling conditions will be ideal for routine clinical application.

생물공정의 측정 및 새로운 공정변수의 개발

  • Heo, Won
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.51-52
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    • 2000
  • 생물공정의 운전에 있어서 적절한 공정변수가 부족한 경우가 많다. 이것은 멸균과정을 견딜 수 있는 신뢰성 높은 센서가 부족하기 때문이다[1]. 생물공정에 주로 사용되는 센서로서는 온도, pH, D.O., rpm, viscosoty 등이 있으나 이 센서들은 배양액의 물리적 혹은 화학적 상태를 측정할 수 있는 경우가 대부분이다[2]. 미생물의 대사활동과 관련이 있는 공정 변수로는 배출가스의 성분을 측정하여 얻을 수 있는 Oxygen uptake rate, Carbon dioxide evolution rate 및 Respiratory quotient가 있으며 현재 생물공정의 운전에 사용되고 있다[3]. 그러나 반복적인 센서의 보정과 연결관의 잦은 청소 및 보수를 필요로 하여 제한적으로 사용되고있는 실정이다. 자동화된 습식분석장치, Gas chromatograph, High Performace Liquid Chromatograph 혹은 Mass spectrophtometry 등을 온라인 샘플 처리장치와 연결하여 발효조의 배양액의 성분을 온라인으로 분석하고 공정의 운전에 응용하는 사례가 많이 발표되었다[4-6]. 고가의 장비 및 운전의 번거러움이나 추가적인 인력이 필요하므로 역시 특별한 경우에만 사용되고 있다. 이외에도 여러 종류의 온라인 센서 및 바이오 센서등이 개발되어 사용되고 있으나 역시 그 사용범위는 특수한 영역에 한정되어있다. 이와 같이 새로운 센서를 개발하여 공정변수를 측정하려는 시도중의 하나가 소프트웨어 센서의 개발이다. 이 것은 공정상에서 발생하는 1차 공정변수를 이용하여 배양액의 상태 혹은 2차적인 공정 변수를 추측해내는 것이다. 대부분의 경우 기존의 공정 변수를 사용하므로 추가적인 비용이 들지 않고 소프트웨어의 형태로 구현되므로 센서의 보정과 설치 및 유지관리의 노력이 매우 적은 장점이 있다. 본 연구에서는 생물공정에서 자동제어 과정에서 발생하는 여러 가지 공정상의 제어 신호로부터 새로운 공정 변수를 얻어내고자 시도하였다. 대부분의 생물공정에서는 pH의 자동제어가 필수적인데 자동제어 과정에서 발생하는 pH 제어 신호 및 pH의 변화 응답신호를 이용하여 배지의 완충용량의 변화와 알칼리의 소비속도를 온라인으로 측정할 수 있었다. 여기에 인공지능망을 설계하여 균체의 량을 온라인으로 추정하는 방법을 개발하였다 [7].산업용 발효조의 운전 온도는 주로 냉각수의 단속적인 공급에 의하여 항상 일정하게 조절된다. 따라서 냉각수의 냉각량을 측정하면 미생물의 배양시 발생하는 대사열량을 측정할 수 있게 된다. 본 연구에서는 실험실의 발효조를 냉각수의 단속적인 공급에 의하여 자동온도 조절이 되도록 개조하고 여기에 냉각수의 유출입 지점에 온도센서를 부착하여 냉각수의 온도를 측정하고 냉각수의 공급량과 대기의 온도 등을 측정하여 대사열의 발생을 추정할 수 있었다. 동시에 이를 이용하여 유가배양시 기질을 공급하는 공정변수로 사용하였다 [8]. 생물학적인 폐수처리장치인 활성 슬러지법에서 미생물의 활성을 측정하는 방법은 아직 그다지 개발되어있지 않다. 본 연구에서는 슬러지의 주 구성원이 미생물인 점에 착안하여 침전시 슬러지층과 상등액의 온도차를 측정하여 대사열량의 발생량을 측정하고 슬러지의 활성을 측정할 수 있는 방법을 개발하였다.

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A study on inspection methods for waste treatment facilities(II): Derivation of problems and improvement direction in inspection methods (폐기물처리시설의 세부검사방법 마련연구(II): 세부검사방법 문제점도출 및 개선방향 설정)

  • Pul-Eip Lee;Eunhye Kwon;Jun-Ik Son;Jun-Gu Kang;Taewan Jeon;Dong-Jin Lee
    • Journal of the Korea Organic Resources Recycling Association
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    • v.31 no.1
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    • pp.85-100
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    • 2023
  • In this study, in order to improve the installation periodical inspection method of waste treatment facilities, we conducted on-site surveys of waste treatment facilities classified into six fields, grasped the problems of inspection methods, and made improvements accordingly. And revised the inspection method for waste treatment facilities. As a result, in the field of incineration and incineration heat recovery, inspection methods such as total temperature measurement and one-year TMS data comparison using a thermal imaging camera were established. And for the safety of the inspected person, it was applied so that the waste can be replaced with a document without opening it. In the case of landfill facilities, the details regarding the use of video information processing equipment and the management of facilities covering the upper part of the landfill facility are presented in the law, but the items that do not have a inspection methods were applied to the inspection method. In the case of Food Waste Treatment Facility, inspection methods were put in place to ensure compliance with standards for foul-smelling fish in odor control, a major cause of complaints. As a result, 10 out of 18 improvement proposals were reflected in the incineration and sterilization grinding, cement kiln, and incineration heat recovery facilities, and 11 out of 12 improvement proposals were reflected in the landfill facility. In the case of food distribution waste treatment facilities, 10 out of 12 improvement proposals were reflected, and a total of 31 inspection methods were improved.