• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.5
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• pp.593-600
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• 1999

The survival rates of Littorina brevicula exposed to experimental concentration regimes of TBTCl, HB and Cd on the large and the small size individuals during 80 days were $80\%$ at 0,9ppb TBTCl, 40 and $25\%$, respectively at 200ppb Hg, and 75 and $45\%$, respectively at 100ppb Cd. The growth rates of the experimental animals exposed to each concentration for 80 days was 0.023mm/day at control, 0.019mm/day at 0.1ppb and 0.014mm/day at 0.9ppb TBTCl, 0.022 mm/day at 5ppb, 0.008 mm/day at 200ppb Hg, and 0.017 mm/day at 5ppb, 0.008mm/day at 100ppb Cd. The respiration rates and excretion rates of the experimental animals exposed to chronic concentration of TBTCl, Hg and Cd were decreased until approximatively 40 days and increased after, Toxic effect of pollutants on L. brevicula was highest at TBTCl. The histological injury of L. brevicula exposed to TBTCl, Hg and Cd was shown at gill, digestive organ and muscle, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.25 no.3
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• pp.236-240
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• 1992

To obtain the applicable data for fish oil utilization, the characteristics of filefish viscera oil were studied. The lipid content showed the lowest value$(38.8\%)$ from viscera of filefish caught in April and the highest value$(59.5\%)$ in July. The seasonal variation in fatty acids showed a tendency of high level of polyenes such as 20:5 and 22:6 and low level of saturates such as 16:0 and 18:0 during the spawning season. While level of monoenes such as 18:1 was low regardless of seasons. The viscera of filefish caught in April contained $46.7\%$ total lipid(TL) which consisted of $94.3\%$ neutral lipid(NL), $1.6\%$ glycolipid(GL) and $4.1\%$ phospholipid(PL). The NL of filefish viscera mainly consisted of triglyceride$(88.1\%)$ and had esterified sterol and hydrocarbon, free sterol, diglyceride and free fatty acid in less quantity. The major fatty acids in TL and NL were 16:0, 18:0, 18:1, 20:5 and 22:6.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.5
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• pp.618-623
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• 1999

A relationship between SST (Sea Surface Temperature) fronts and formation of fishing grounds was examined using the data on fishing conditions obtained from 41 Korean purse-seiners during the period of 1991 to 1996. Good fishing grounds observed in the southern sea of Korea and the nothern area of the East China Sea were yearly found around the frontal zone and around the marginal area of Tsushima Current which was the periphery of fronts, Also, there were several fishing grounds, which are not related to the fronts. They can be classified into the following four types : The first type was found in the warm water pocket located in the western area of Cheju Island in winter. The second type was made in a intensive bending of isobathytherm with a higher temperature in the main stream of Tsushima Current between Cheju Island and the Goto Islands in winter. The third type was formed by the topographical vortex motion near the Tsushima Island in winter and spring. The fourth type was found at the area of the reflow Sea Warm Current in southwest sea of Korea between the costal front zone and the Yellow Bottom Cold Waters in summer and autumn.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.4
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• pp.432-437
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• 1999

Age and growth of the marbled rockfish, Sebastiscus marmoratus were studied based on 818 specimens caught from the coastal waters of Cheju Island from July 1992 to July 1993 and from June 1994 to July 1995. According to the monthly changes of marginal increment in each annual ring groups, the ring of otolith was formed in both female and male in February. Relationship between the total body length (TL) and the radius of otolith (R) was estimated, Growth of females was rather slower than that of male in total length; TL=-0.526+4.818R(r=0.847) for female, and TL=-1.895+ 5.239R (r=0.881) for male. The growth curve fits well with Bertalanffy equation: $L_{t}=21.484(1-e^{-0.424(t+0.334)})$ for female and $L_{t}=23.698(1-e^{-0.441(t+0.0589)})$ for male, Growth in weight as follows: $W_t=163.42(1-e^{-0.424(t+0.334)})^3$ for female and $W_t=210.14(1-e^{-0.441(t+0.0589)})^3$ for male.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.4
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• pp.444-451
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• 1999

The effect of individual density on the growth and survival of Laminaria japonica was experimentally evaluated. Four density treatments of young sporophytes (0.63 cm In maximum length) grown in the laboratory were transplanted to the southeastern coast of Korea and their survivorship and growth rate were measured twice a month from January to July, 1996. Plants showed higher survivorship at low density. Final densities at three lowest densities were similar, ranging from 34 to 38 inds${\cdot}100 cm^{-2}$. Planis at the highest density, however, suffered greater decline than at lower densities, and only 21 plants remained at the end of this study. Mean frond size (length and width) fluctuated throughout the study period at four densities. During the first two mouths after outplanting, mean frond size was negatively correlated with initial density, However, the size was density independent after three months. As stand density increased, size-frequency distribution through time tended to be more positively skewed. The 10 largest plants grew faster in frond size and attained larger size in the lower density. There was a positive relationship between plant size and relative growth rate (or survival). The slope of yield-density (Y-D) relationships at four densities ranged from -0.060 to -0.137. The study suggests that density can strongly affect the survival and growth of this alga. The density-dependent survival and growth patterns, however, were not strictly consistent with those of terrestrial plants.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.12 no.4
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• pp.724-729
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• 2008

In the case of 'MAEMI', the Typhoon which formed in September, 2003, the largest-scale damage of tidal wave was caused by the co-occurrence of Typhoon surge and full tide. Until now Korea has been focusing on the calculating the amount of damage and its restoration to cope with these sea and harbor disasters. It is essential to establish some systematic counterplans to diminish such damages of large-scale tidal invasion on coastal lowlands considering the recent weather conditions of growing scale of typhoons. Therefore, the purpose of this research is to make the counterplans for prevention against disasters fulfilled effectively based on the data conducted by comparing and analyzing the accuracy between observation values and the results of estimating the greatest overflow area according to abnormal tidal levels centered on Masan area where there was the severest damage from tidal wave at that time. It's necessary utilize data like high-resolution satellite image and LiDAR(etc.) for correct analysis data considering geographical characteristics of dangerous area from the storm surge. And we must make a solution to minimize the damage by making data of dangerous section of flood into GIS Database using those data (as stated above) and drawing correcter damage function.

• Journal of agriculture & life science
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• v.46 no.1
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• pp.163-176
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• 2012

TTo increase productivity of a strong extracellular alkaline protease (BCAP), stable strains of Bacillus clausii I-52 carrying another copy of BCAP gene in the chromosome were developed. Integrative vector, pHPS9-fuBCAP carrying BCAP promoter, ribosome binding site, signal sequence and active protease gene was constructed and transferred into B. clausii I-52, and integration of the constructed plasmid into chromosome was identified by PCR. An investigation was carried out on BCAP production by B. clausii I-52 and transformant C5 showing the highest relative activity of alkaline protease using submerged fermentation. Maximum enzyme activity was produced when cells were grown under the submerged fermentation conditions at $37^{\circ}C$ for 48 h with an aeration rate of 1 vvm and agitation rate of 650 rpm in a optimized medium (soybean meal 2%, wheat flour 1%, sodium citrate 0.5%, $K_2HPO_4$ 0.4%, $Na_2HPO_4$ 0.1%, NaCl 0.4%, $MgSO_47H_2O$ 0.01%, $FeSO_47H_2O$ 0.05%, liquid maltose 2.5%, $Na_2CO_3$ 0.6%). A protease yield of approximately 134,670U/ml was achieved using an optimized media, which show an increase of approximately 1.6-fold compared to that of non-transformant (83,960 U/ml). When the stability of transformant C5 was examined, the integrated plasmid pHPS9-fuBCAP was detected in the transformant after cultivation for 8 days, suggesting that it maintained stably in the chromosomal DNA of transformant C5.

• Journal of agriculture & life science
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• v.43 no.6
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• pp.95-103
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• 2009

To develop natural intermediate flavoring substances, optimal hydrolysis conditions and taste compounds for two step enzyme hydrolysate(TSEH) using Grass puffer(Takifugu niphobles) were investigated. The optimal conditions for TSEH method were revealed in temperature at $55^{\circ}C$ 3 hour digestion with Alcalase (pH 7.5) at the 1st step and 2 hour at $45^{\circ}C$ digestion with Flavourzyme(pH 6.0~6.5) at the 2nd step. TSEH method was superior to hot-water extraction on the aspect of yield, nitrogen contents and organoleptic taste quality such as umami and control of bitter taste formation. In taste active-components in Glass puffer TSEH, total free amino acid content was 4,502 mg%, major free amino acids were Pro, Leu, Lys, Hypro, Tau, Arg, Phe, Ala, Glu and Val in ordor. As for nucleotides, IMP(372 mg%) was the principal component and also contents of TMAO, total creatinine, and betaine were 43, 278 and 41 mg% in Glass puffer TSEH, respectively. The major inorganic ions in TSEH were Na(949 mg%), K(222 mg%), Cl(1,180 mg%) and $PO_4$(1,081 mg%).

• Journal of agriculture & life science
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• v.45 no.6
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• pp.201-212
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• 2011

The alkaline protease gene was cloned from a halo-tolerant alkalophilic Bacillus clausii I-52 isolated from the heavily polluted tidal mud flat of West Sea in Inchon Korea, which produced a strong extracellular alkaline protease (BCAP). Based on the full genome sequence of Bacillus subtilis, PCR primers were designed to allow for the amplification and cloning of the intact pro-BCAP gene including promoter region. The full-length gene consists of 1,143 bp and encodes 381 amino acids, which includes 29 residues of a putative signal peptide and an additional 77-amino-acid propeptide at its N-terminus. The mature BCAP deduced from the nucleotide sequence consists of 275 amino acids with a N-terminal amino acid of Ala, and a relative molecular weight and pI value was 27698.7 Da and 6.3, respectively. The amino acid sequence shares the highest similarity (99%) to the nattokinase precursor from B. subtilis and subtilisin E precursor from B. subtilis BSn5. The substrate specificity indicated that the recombinant BCAP could hydrolyze efficiently the synthetic substrate, N-Suc-Ala-Ala-Pro-Phe-pNA,and did not hydrolyze the substrates with basic amino acids at the P1 site. The recombinant BCAP was strongly inhibited by typical serine protease inhibitor, PMSF, indicating that BCAP is a member of the serine proteases.

• Korean Journal of Remote Sensing
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• v.18 no.6
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• pp.305-317
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• 2002

The purpose of this study is to analyze the land cover environmental changes in the Anmyeon-do. Especially, it centers on the changes in the land cover environment through methods of GIS and remote sensing. The land cover environmental change areas were detected from remote sensing data, and geographic data sets related to land cover environment change were built as a spatial database in GIS. Fuzzy logic was applied for data representation and integration of thematic maps. In the natural, social, and economic environment variables, the altitude, population density, and the national land use planning showed higher fuzzy membership values, respectively. After integrating all thematic maps using fuzzy logic operation, it is possible to predict the change quantitatively. In the study area, a region where land cover change will be likely to occur is the one on a plain near the shoreline. In particular, the hills of less than 5% slope and less than 15m altitude, adjacent to the ocean, were quite vulnerable to the aggravation of coastal environment on account of current, large-scale development. In conclusions, it is expected that the generalized scheme used in this study is regarded as one of effective methodologies for land cover environmental change detection from geographic data.