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Lowering the Bitterness of Enzymatic Hydrolysate Using Aminopeptidase-active Fractions from the Common Squid (Todarodes pacificus) Hepatopancreas (살 오징어(Todarodes pacificus) 간췌장으로부터 aminopeptidase 활성 획분의 쓴맛 개선 효과)

  • Kim, Jin-Soo;Kim, Hye-Suk;Lee, Hyun Ji;Park, Sung Hwan;Kim, Ki Hyun;Kang, Sang In;Heu, Min Soo
    • Korean Journal of Food Science and Technology
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    • v.46 no.6
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    • pp.716-722
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    • 2014
  • Aminopeptidase-active fractions from crude extract of the hepatopancreas of a common squid (Todarodes pacificus) were obtained using acetone (AC; 30-40%) and ammonium sulfate precipitation (AS; 60-70% saturation), anion exchange (AE-II; 0.2 M NaCl) and gel filtration chromatography (GF-I; 30-50 kDa), respectively. The debittering capacity of GF-I fraction based on the aminopeptidase activity (89.2 U/mg), recovery (56.6%) and sensory evaluation (1.0) was better than that of other fractions. Release of amino acids increased as incubation time was increased, and the bitterness of the enzyme reaction mixtures decreased. Incubation with the GF-I fraction for 24 h resulted in the hydrolysis of several peptides, as revealed by reverse-phase HPLC profiles. Peaks 3, 5 and 6 showed the decreased area (%), whereas peaks 1, 2 and 4 showed the increased area. The GF-I fractions were found to be suitable for reducing bitterness in protein hydrolysates by catalyzing the hydrolysis of bitter peptides.

Physico·Chemical Properties of Organic and Inorganic Materials Used as Container Media (혼합배지 조제에 이용되는 유·무기 물질들의 물리·화학적 특성)

  • Choi, Jong Myung;Chung, Hae Joon;Choi, Jong Seung
    • Horticultural Science & Technology
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    • v.18 no.4
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    • pp.529-535
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    • 2000
  • Organic materials such as composted rice-hull, saw dust, and pine bark, and inorganic materials such as vermiculite, perlite, and recycled rockwool were commonly employed as container media in domestic greenhouse industry. The objective of this research was to get informations in soil physico chemical properties of those materials. Composted dry-peeling bark and wet-peeling bark had 72.1% and 69.1%, respectively, in particles larger than 1.0 mm, which were much higher than 34.7% of composted rice-hull and 33.7% of composted saw dust. Imported vermiculite had 89.9%, but domestic vermiculite had 25.7% in particles larger than 1 mm. In soil physical properties, Russian peat had the highest container capacity of 79.3%, and wet-peeling bark had the lowest container capacity of 58.2%. However, Russian peat and composted saw-dust had 4.1% in air space indicating that possible problems could occur in soil aeration when those are employed for container grown crops. Saw dust had $2.3mS{\cdot}cm^{-1}$ in electrical conductivity, while other composted organic materials had less than $0.25mS{\cdot}cm^{-1}$. Imported vermiculite had 64.0 meq/100 g in cation exchange capacity, which was 2.4 times higher than those of domestic vermiculite, 27.2 meq/100 g. Domestic vermiculite had higher Ca and Mg and less Na contents than those of imported vermiculite.

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The Long-term Durability Evaluation of PC Box for Near-surface Transit System manufactured by Microwave Heat curing (마이크로웨이브 발열양생에 의해 제작된 저심도 철도시스템용 PC BOX의 장기내구성 평가)

  • Koh, Tae-Hoon;Yoo, Han-Ju
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.4
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    • pp.766-771
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    • 2016
  • This study examined the long-term durability of PC boxes, which was manufactured by low-carbon eco-friendly concrete using an alternative binder to cement and alternative fine aggregate to sand and microwave heat curing system to reduce the construction cost of a near-surface transit system. Based on the test results, the initial compressive strength of microwave heat cured concrete was higher than that of the steam cured concrete, but those were similar in the long-term age. In addition, there was no significant difference between the two curing conditions in the chemical resistance and the freeze-thawing resistance, and the chloride ion penetration level of the concrete cured by two methods was very low. Therefore, low-carbon eco-friendly concrete and microwave heat curing technology are expected to contribute to the economic construction of a near-surface transit system, and reduce carbon dioxide emissions and environmental impact.

Semiconductor wafer exhaust moisture displacement unit (반도체 웨이퍼 공정 배기가스 수분제어장치)

  • Chan, Danny;Kim, Jonghae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.8
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    • pp.5541-5549
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    • 2015
  • This paper introduces a safer and more power efficient heater by using induction heating, to apply to the semiconductor wafer fabrication exhaust gas cleaning system. The exhaust gas cleaning system is currently made with filament heater that generates an endothermic reaction of N2 gas for the removal of moisture. Induction theory, through the bases of theoretical optimization and electronic implementation, is applied in the design of the induction heater specifically for the semiconductor wafer exhaust system. The new induction heating design provides a solution to the issues with the current energy inefficient, unreliable, and unsafe design. A robust and calibrated design of the induction heater is used to optimize the energy consumption. Optimization is based on the calibrated ZVS induction circuit design specified by the resonant frequency of the exhaust pipe. The fail-safe energy limiter embedded in the system uses a voltage regulator through the feedback of the MOSFET control, which allows the system performance to operate within the specification of the N2 Heater unit. A specification and performance comparison from current conventional filament heater is made with the calibrated induction heater design for numerical analysis and the proof of a better design.

Characteristics of Diamond Like Carbon Film Fabricated by Plasma Enhanced Chemical Vapor Deposition Method with mixed Ar, N2 gas rate (혼합된 Ar, N2 가스 유량에 따른 PECVD 방법에 의하여 제작된 다이아몬드 상 탄소 박막의 특성)

  • Gang, Seong-Ho;Kim, Byeong-Jin;Bae, Gyeong-Tae;Ju, Seong-Hu
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2018.06a
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    • pp.87-87
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    • 2018
  • 다이아몬드 상 탄소(diamond-like carbon, DLC)는 상당량의 $sp^3$ 결합을 가지는 비정질 탄소(a-C) 또는 수소화 비정질 탄소(a-C:H)로 이루어진 준안정 형태의 탄소이다. DLC는 전기 저항과 굴절률이 높고 화학적으로 다른 물질과 반응하지 않으며, 마찰계수가 낮고 경도가 높아 자기 디스크, 광학 소자 등의 다양한 분야에서 적용되고 있다[1,2]. 또한 다이아몬드에 비해 상온에서 성장이 가능할 정도로 합성온도가 낮아 적용 기판의 제한이 거의 없고, 증착 방법과 조건에 따라 탄소 결합의 다양성과 비정질성이 변화하기 때문에 넓은 범위의 특성을 얻을 수 있는 장점이 있다. 지금까지 DLC 박막의 광학적 특성, 특히 굴절률, 광학적인 에너지 밴드 갭, 자외선과 적외선 투과성에 대해서는 많은 연구가 진행되었으나 가시광선의 투과성에 대한 연구는 제한적이며[4], 가시광선 투과도 개선에 대한 연구는 전무하다. 본 연구에서는 ITO 기판 위에 DLC를 합성하고 기계적 특성과 가시광선 영역 투과도를 조사하였다. RF-PECVD(radio frequency plasma enhanced chemical vapor deposition) 방법에 의해서 $C_2H_2+Ar$ 혼합 가스 비율과 $C_2H_2+N_2$ 혼합 가스 비율을 변화시켜 ITO 기판 위에 DLC 박막을 합성하였다. 공정 압력과 rf-power, 증착시간, 기판온도는 0.2 torr, 40 W, 5 분, $50^{\circ}C$로 고정하고, 공정 가스는 $C_2H_2+Ar$$C_2H_2+N_2$가 200 sccm이 되도록 비율을 변화하였다. $C_2H_2:Ar$$C_2H_2:N_2$의 비율은 180 : 20, 160 : 40, 140 : 60, 120 : 80, 100 : 100이 되도록 가스의 유량을 조절하였다. 투과도는 가시광선(380 ~ 780 nm) 범위에서 측정하였고 두께와 표면조도는 AFM으로 측정하였다. 투과도는 $C_2H_2+Ar$의 Ar 가스 비율이 증가할수록 증가해 140 : 60일 때 최댓값을 나타낸 후 다시 감소하였다. $C_2H_2+N_2$ 투과도는 $N_2$ 가스 비율이 증가할수록 감소하는 경향을 나타내었다. 표면 거칠기는 $C_2H_2+Ar$ 혼합 가스를 사용한 경우의 Ar의 가스 비율이 증가할수록 증가하였다. 그러나 $C_2H_2+N_2$ 혼합 가스를 사용한 경우에는 $N_2$ 가스의 혼합 비율이 증가할수록 감소하였다.

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Effect of applied current density on the corrosion damage with galvanostatic corrosion experiment of aluminum alloy for ship (선박용 알루미늄 합금의 정전류 부식 시험에 의한 부식 손상에 미치는 인가 전류밀도의 영향)

  • Kim, Yeong-Bok;Park, Il-Cho;Lee, Jeong-Hyeong;Kim, Seong-Jong
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2018.06a
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    • pp.106-106
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    • 2018
  • 해양환경용 선박재료는 전기화학적인 부식을 발생시키는 염소이온($Cl^-$)이 다량 포함된 부식 환경에 장기간 노출되어 있어 부식에 대해 취약하다. 따라서 우수한 내식성 및 내침식성을 가진 재료를 선정하는 것은 매우 중요하다. 알루미늄 합금은 충분한 강도와 부동태 피막 형성으로 인해 내식성이 우수하여 해양환경용 선박 재료로서 널리 이용되고 있으며, 이에 따른 부식 특성에 관한 연구도 활발히 이뤄지고 있다. 그러나 선박에서는 부식에 의한 손상뿐만 아니라 전식에 의한 부식 손상도 발생할 수 있다. 특히 선미 부분은 프로펠러의 동합금과 알루미늄 합금의 이종금속 간 전위차에 의한 전식이 발생하여 선체의 다른 부위에 비해 부식이 더 심하게 진행될 수도 있다. 또한 전식은 해안 부두에 접안된 선박의 용접 시미주전류(stray current)에 의한 부식손상이 발생할 수 있으나 이에 대한 연구는 미미한 실정이다. 따라서 본 연구는 해양환경에서의 전식을 인위적으로 모사할 수 있는 부식 정전류 시험법을 이용하여 다양한 크기의 전식 손상을 유발시켰으며, 해양환경 하에서 선박재료로 주로 사용되는 알루미늄 합금인 Al5083-H321, Al5052-O, Al6061-T6에 대한 전식 특성을 비교, 분석하였다. 실험 방법으로 작동전극은 각 재료의 시험편을 $2cm{\times}2cm$ 으로 절단하여 sand paper # 2000 번까지 연마 후 아세톤과 증류수로 세척하고 건조하였으며, 제작된 시험편은 자체 제작한 홀더를 이용하여 $1cm^2$만 노출시킨 후 정전류 가속 실험을 실시하였다. 기준전극은 은/염화은(Ag/AgCl) 전극을, 대응전극은 백금(Pt) 전극을 사용하였다. 정전류 가속 조건은 $0.001mA/cm^2$, $0.1mA/cm^2$, $1mA/cm^2$, $5mA/cm^2$, $10mA/cm^2$의 전류 밀도를 천연해수에서 30분간 인가하였다. 각 재료에 대한 전식 특성은 실험 전후의 무게 감소량으로 전식의 저항 특성을 확인하였다. 그리고 3D 현미경으로 표면 손상 경향과 깊이를 측정하였으며, 주사전자현미경 (SEM)을 통해 표면 형상을 미시적으로 관찰하였다. 부식 정전류 시험 결과 모든 시편에서 $0.01mA/cm^2$에서 미세한 국부적인 부식이 일어났으며, 전류밀도가 증가할수록 표면 전반에 부식이 진행되고 성장하였다. 그리고 모든 인가 전류밀도의 조건에서 Al6061-T6가 5000계열(Al5083-H321, Al5052-O)보다 더 우수한 내식성을 나타났다.

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Thermal Compression of Copper-to-Copper Direct Bonding by Copper films Electrodeposited at Low Temperature and High Current Density (저온 및 고전류밀도 조건에서 전기도금된 구리 박막 간의 열-압착 직접 접합)

  • Lee, Chae-Rin;Lee, Jin-Hyeon;Park, Gi-Mun;Yu, Bong-Yeong
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2018.06a
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    • pp.102-102
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    • 2018
  • Electronic industry had required the finer size and the higher performance of the device. Therefore, 3-D die stacking technology such as TSV (through silicon via) and micro-bump had been used. Moreover, by the development of the 3-D die stacking technology, 3-D structure such as chip to chip (c2c) and chip to wafer (c2w) had become practicable. These technologies led to the appearance of HBM (high bandwidth memory). HBM was type of the memory, which is composed of several stacked layers of the memory chips. Each memory chips were connected by TSV and micro-bump. Thus, HBM had lower RC delay and higher performance of data processing than the conventional memory. Moreover, due to the development of the IT industry such as, AI (artificial intelligence), IOT (internet of things), and VR (virtual reality), the lower pitch size and the higher density were required to micro-electronics. Particularly, to obtain the fine pitch, some of the method such as copper pillar, nickel diffusion barrier, and tin-silver or tin-silver-copper based bump had been utillized. TCB (thermal compression bonding) and reflow process (thermal aging) were conventional method to bond between tin-silver or tin-silver-copper caps in the temperature range of 200 to 300 degrees. However, because of tin overflow which caused by higher operating temperature than melting point of Tin ($232^{\circ}C$), there would be the danger of bump bridge failure in fine-pitch bonding. Furthermore, regulating the phase of IMC (intermetallic compound) which was located between nickel diffusion barrier and bump, had a lot of problems. For example, an excess of kirkendall void which provides site of brittle fracture occurs at IMC layer after reflow process. The essential solution to reduce the difficulty of bump bonding process is copper to copper direct bonding below $300^{\circ}C$. In this study, in order to improve the problem of bump bonding process, copper to copper direct bonding was performed below $300^{\circ}C$. The driving force of bonding was the self-annealing properties of electrodeposited Cu with high defect density. The self-annealing property originated in high defect density and non-equilibrium grain boundaries at the triple junction. The electrodeposited Cu at high current density and low bath temperature was fabricated by electroplating on copper deposited silicon wafer. The copper-copper bonding experiments was conducted using thermal pressing machine. The condition of investigation such as thermal parameter and pressure parameter were varied to acquire proper bonded specimens. The bonded interface was characterized by SEM (scanning electron microscope) and OM (optical microscope). The density of grain boundary and defects were examined by TEM (transmission electron microscopy).

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An Experimental Study on the Fire Monitoring System for Tunnel Using SMA and Fiber Optic Cable (형상기억합금과 광케이블을 이용한 터널의 화재감지 시스템 개발에 관한 실험적 연구)

  • Hwang, Ji-Hyun;Park, Ki-Tae;Lee, Kyu-Wan
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.18 no.5
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    • pp.128-134
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    • 2014
  • Recently, design and construction of street tunnels tend to focus on cost reduction and preservation of nature. Accordingly, research is actively being carried out to quickly detect fires when they occur in tunnels, which have partially closed structures. Among such research, fire detection methods using optical fiber sensors have a wide bandwidth and fast transmission speed, while using light as a medium. Therefore, it does not receive electrical interference and there is almost no loss of information during transmission, while also having little noise as well. In relation to this, a fire monitoring system that can accurately detect the location of fires in real time using shape memory alloy and optical cables was developed in this study. In order to verify the developed method, light loss measurement test was conducted according to indoor temperature changes, while also conducting fire simulation tests by installing test beds in common underground zones with different external environments of temperature and distance. Upon carrying out experiments, the fire monitoring system developed in this study was found to be able to detect fires in long distance sections in real time.

AN ELECTROCHEMICAL STUDY ON THE CORROSION BEHAVIOUR OF AMALGAMS IN THE SALINE SOLUTIONS WITH FLUORIDE COMPOUNDS (불소화합물을 첨가한 생리식염수에서 아말감의 부식거동에 관한 전기화학적 연구)

  • Yun, Chong-Hun;Kwon, Hyuk-Choon;Um, Chung-Moon
    • Restorative Dentistry and Endodontics
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    • v.22 no.1
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    • pp.396-412
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    • 1997
  • The purpose of this study is to observe the corrosion characteristics of five dental amalgams(CAULK FINE CUT, OPTALLOY II, DISPERSALLOY, TYTIN, and VALIANT) in the solutions with fluoride compound through the anodic polarization curve obtained by using a potentiostat. After each amalgam alloy and Hg being triturated, the triturated mass was inserted into the cylindrical metal mold, and condensed by hydraulic pressure(160 kg/$cm^2$). Each specimen was removed from the metal mold. Specimens were polished with the silicone carbide grinding paper 24 hours after condensation and stored at room temperature for 1 week. The anodic polarization curves were employed to compare the corrosion behaviours of the amalgams in 0.9 % saline solution and in the saline solutions with 2.2 ppm, 0.05 %, 2 % NaF, and 8 % $SnF_2$ at $37^{\circ}C$ with 3-electrode potentiostat. After the immersion of specimen in electrolyte for 30 minutes, the potential scan was begun. The potential scan range was -1500m V to + 800m V(vs. S.C.E.) in the working electrode and the scan rate was 50 mV/sec. The results were as follows, 1. The corrosion potential, the potential of anodic current peak, and transpassive potential in the saline solutions with NaF shifted to lower direction than those in normal saline solution, and the current density in the saline solutions with NaF was higher than that in normal saline solution. The differences were increased as the concentrations of NaF became higher. 2. The corrosion potential and transpassive potential in the saline solution with $SnF_2$ shifted to higher direction than those in normal saline solution, and the current density in the saline solution with $SnF_2$ was higher than that in normal saline solution after the corrosion potential. The anodic polarization curves in the saline solution with $SnF_2$ had no outstanding anodic current peak. 3. The corrosion potentials for high-copper amalgams were much higher than those for CAULK FINE CUT and OPTALLOY II in normal saline solution, but, as the concentrations of fluoride compound became higher, the differences in corrosion potentials between them were decreased. The corrosion potentials had the similarity in the saline solution with 2% NaF or 8% $SnF_2$. 4. The current density for TYTIN was the lowest among the others in normal saline solution and in the saline solution with 2.2 ppm or 0.05 % NaF. 5. There was no significant difference in current density between Pd-enriched VALIANT and other high-copper amalgams.

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An Experimental Study of Wave Overtopping Characteristics on the Structure for Wave Overtopping Power Generating System (월파형 파력발전구조물의 월파 특성에 관한 실험적 연구)

  • Shin, Seung-Ho;Hong, Key-Yong
    • Journal of Navigation and Port Research
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    • v.30 no.8 s.114
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    • pp.649-655
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    • 2006
  • Waves progressing into the coastal area can be amplified, swashed and overtopped by a wave overtopping control structure, and it converts the kinetic energy of the waves to the potential energy with a hydraulic head above the mean sea level by conserving the overflow in a reservoir. Then the potential energy in the form of hydraulic head can be converted to electric power utilizing extremely low-head hydraulic turbine. This study aims to find the most optimal shape of wave overtopping structure which maximizes overtopping volume rate of sea water. Laboratory experiments for the performance evaluation of wave overtopping control structures were carried out in three dimensional wave tank, and the three dimensional structure models with planar wave concentration shapes(B/b) were manufactured into five classes, which were optimized by cross sectional parameters of the structure, ie, length of ramp(l), gradient of inclined ramp($cot{\phi}$) and freeboard height of the wave overtopping structure($h_e$) proposed by Shin and Hong(2005). The wave overtopping discharges were investigated with 20 incident wave conditions and wave directions of $0^{\circ},\;15^{\circ},\;30^{\circ}$.