• Korean Journal of Soil Science and Fertilizer
• /
• v.45 no.3
• /
• pp.325-331
• /
• 2012

The dual inoculation response of soybean with rhizobium and mycorrhiza was examined in pot vermiculite and field soils. In order to select a symbiotically compatible mycorrhiza with Bradyrhizobium japonicum, a highly germinating spore among 60 strains from 32 upland soils in southern part of Korea was obtained in Acaulospora sp., Gigaspora sp. and Glomus sp., respectively. As a result of dual inoculation of Glycin max cv. Dajangkong and Eunhakong both with $1{\times}10^8$cells of B. japonicum YCK 213 and 10 spores of each mycorrhiza in vermiculite pot, only Glomus sp. treatment together with the rhizobium showed significant increase ($P{\leqq}0.05$) both in shoot dry wt and nodule mass of not Eunhakong but Dajangkong. In red-yellow soils with pH 5.2($1:5H_2O$) and 203 mg of Lancaster P per kg of soil, in which $10^3$ cells of B. japonicum and $10{\pm}0.2$ spores of mycorrhizae per gram of soil were naturalized, grain yield of G. max cv. Dajangkong was increased to 3.9% by dual inoculation both of $4.8{\times}10^6$cells of B. japonicum and 10 spores of mycorrhizae per two seeds under condition applied with 30 kg $P_2O_5$ and 34 kg $K_2O$ per hectare compared to conventionally fertilized plot (2.75 MT $ha^{-1}$) added with 30 kg N $ha^{-1}$. However, there was not significant.

• Journal of Life Science
• /
• v.7 no.3
• /
• pp.167-171
• /
• 1997

As an initial effort to elucidate RNA: protein binding in a way to regulate translation initiation and phosphorylation, a cDNA encoding a double-stranded RNA binding factor (RBFII)was isolated from Hela ZAPII cDNA library by affinity screening using [$\alpha$$^{-32}$P] UMP-labeled HIV Rev-responsive element(RRE) RNA. The nucleotide sequence of RBF (or TRBP) cDNA except the 5’end. At the 5’end, This common ORF was fused in-frame to N-terminal residues of Lac-Z through a unique 138 nt sequence encoding 46residues in the case of RBFII and a 63 nt sequence encoding 21 residuces in the case of RBFI. The context of ATG appearing first in the sequences suggests that both these cDNA inserts are incomplete at the 5’end.

• Applied Biological Chemistry
• /
• v.32 no.2
• /
• pp.109-115
• /
• 1989

This study was aimed to find out the influence of long-term fertilization for 21 years on soil enzyme activities in the silty clay loam textured normal paddy soil. Total urease activity (TUA) and the microbial urease activity (MUA) were shown to be changed significantly, but the accumulated urease activity (AUA) was similar within trial plots. Especially the MUA of the plots annually applied N.P.K. fertilizers with compost and N.P.K. fertilizers with silicate fertilizer were the highest among plots. The total L-glutaminase activity (TGA) and the accumulated L-glutaminase activity(AGA) were changed significantly among trial plots, but the microbial L-glutaminase activity (MGA) was not. By the simple correlation analysis, it was shown that the TGA and the AGA correlated highly significant to available phosphorus available $SiO_2$ content and pH. Addition of the toluene to the incubation mixture did not markedly affect the activity of phosphatase, but the difference of phosphatase activity among plots was significant. By the simple correlation analysis, it was shown that the phosphatase activity ; correlated highly significant to pH, available $SiO_2$, available phosphorus and exchangeable calcium in soils.

• Journal of Animal Environmental Science
• /
• v.18 no.2
• /
• pp.63-74
• /
• 2012

This experiment was carried out to investigate the effect of different stocking rate on growth, cast production and conversion efficiency of organic matter to tissues of earthworm. The carbon and nitrogen ratio (C/N) of tested Korean cow manure was 25.1, it was estimated an adequate ratio as feed for earthworms. The different stocking rates were 1:8(S-1), 1:16(S-2), 1:32(S-3) 1:64(S-4) 1:128(S-5) and 1:256 (S-6) as the ratios of earthworm fresh weight to biomass of Korean cow manure, respectively. A stocking rate of 1:32(S-3) was obtained a significantly highest values of increasing rate and conversion efficiency of organic matter to earthworm tissues. The mean values of increasin g rate of fresh weight and conversion efficiency of organic matter to earthworm tissues were 10.63 mg/day and 6.65% at the ratio of 1:32(S-3) with a rearing volume was $56.6cm^3$. A stocking rate of 1:8(S-1) was obtained a highest ratio of vermicasts, but showed a negative values of increasing rate and conversion efficiency of organic matter to earthworm tissues, it may due to severely food competition between individuals during the rearing periods. The pH, total nitrogen, available phosphorus, cation exchange capacity and exchangeable cations of vermicasts tended to increase with stocking rate. Especially, available phosphorus, cation exchange capacity and exchangeable cations of vermicasts tended to increase with rearing progressed. Vermicasts have the potential for improving plant growth when amended to container medium and soil according to increased availability of nutrients and improved physicochemical properties.

• The korean journal of orthodontics
• /
• v.29 no.5 s.76
• /
• pp.613-625
• /
• 1999

The purpose of this study was to compare the bracket shear bond strengths of the crystal growth solutions with those of the $37\%$ phosphric acid etch technique. The 4 crystal growth solutions were made experimentally in the lab, that is, (1) $30\%$ polyacrylic acid solution containing 0.3 M sulfuric acid (ES 1), (2) $30\%$ polyacrylic acid solution containing 0.6M sulfuric acid (ES 2), (3) $30\%$ polyacrylic acid solution containing 0.3 M sulfuric acid and 0.6 M lithium sulfate(ES 3), and (4) $30\%$ polyacrlic acid solution containing 0.3 M sulfuric acid and $5\%$ phosphoric acid(ES4). The $37\%$ phosphoric acid solution used as a control. Bovine lower incisor tooth enamel was treated by the above solutions for 60 sec, washed out for 20 sec with slow water stream, and bonded lower anterior edgewise bracket with the light curing orthodontic composite resin adhesives. The teeth bonded brackets were stored in the distilled water at room temperature for 24 h, and followed to test the bracket shear bond strength. The acid etch technque showed 177.6 kg/$cm^2$ of mean shear bond strength which was the highest among the enamel treatment solutions. ES 1 shown 58.4 kg/$cm^2$ of mean shear bond strength and that of ES 4 showed 66.5 kg/$cm^2$. There was no significant difference between the two(p>0.05). ES2 showed 110.6kg/$cm^2$ of mean shear bond strength which was $62.3\%$ of that of acid etch technique. ES 3 showed 131.1 kg/$cm^2$ of mean shear bond strength which was the highest among experimental crystal growth solutions and which was $74\%$ of that of acid etch technique. The shear bond strengths of the crystal growth solutions were significantly lower that that of acid etch technique(p<0.05). The results sugest that although bracket shear bond strength of $30\%$ polyacrylic acid solution containing 0.3M sulfuric acid and 0.6 M lithium sulfate were showed the highest, it is low for the clinical application of this solution.

• Restorative Dentistry and Endodontics
• /
• v.24 no.1
• /
• pp.147-155
• /
• 1999

The purpose of this study was to investigate the effect of phosphoric acid concentration on the movement of 2-hydroxyethylmethacrylate(HEMA) from bonding resin - resin composite combination through dentin in vitro. Freshly extracted human third molar teeth were divided into four groups each of 10 teeth. A closed chamber with 1 ml distilled water was attached to the CEJ of each tooth. An occlusal cavity of 4mm diameter & remaining dentin thickness of 1.0-1.5mm was prepared in each tooth. Dentin was treated with 10% phosphoric acid gel for 15 seconds. 32% phosphoric acid gel for 15 seconds, or with 35% phosphoric acid gel for 15 seconds. A control group not treated with acid gel was also prepared. The cavities were rinsed, dried and then treated with the HEMA-containing All-Bond 2 primer & bonding resin which was light-cured for 10 seconds. The cavities were then restored with Z100 composite resin(shade:A3.5:3M Dent. Prod. USA) & light cured for 30 seconds. Water samples were retrieved from the chambers over a time course (4.32, 14.4, 43.2, 144 & 432 minutes ; 1, 3 & 10 days) and analyzed by high performance liquid chromatography. The results were as follows. 1. HEMA was detected in the pulp chambers of all teeth from 4.32 minutes after resin placement The highest rate of release was in the first sample period (0-4.32 min) & rate of release declined exponentially thereafter. 2. No significant differences were found for mean release rate for HEMA over a time course among the four groups (p>0.05). 3. The diffusion rate was significantly (p<0.05) less for 10% phosphoric acid gel than 32% phosphoric acid gel at the second sample period(4.32-14.4 min). 4. No significant differences were found for cumulative HEMA diffusion among the four groups at 10 days(p>0.05) and mean total(cumulative) release at 10 days for all groups was in the 9 - 16 nmol range. 5. The cumulative release was significantly (p<0.05) less for 10% phosphoric acid gel than 32% phophoric acid gel at the third(14.4-43.2 min) & fourth(43.2-144 min) sample period.

• Journal of Korean Ophthalmic Optics Society
• /
• v.17 no.2
• /
• pp.233-239
• /
• 2012

Purpose: In the study, the change of protein and lipid deposits on soft contact lens by drinking was investigated. Methods: Fifty male subjects wearing soft contact lens were surveyed whether they felt any discomfort induced by drinking or not. Further, 32 male subjects who has no ocular disease drank 190 mL alcohol. The protein and lipid deposits on soft contact lens (etafilcon A material) of subjects were measured after 4 hours later and compared with those of non-drinking subject. Results: When subjects drink alcohol with soft contact lens on, 58% of subjects answered they experienced the change of lens awareness such as stiffness, blurry sight, dryness and so on. The protein deposit on soft contact lens increased an average of $59.3{\mu}g/lens$ by drinking and the case of more than double in protein deposit was reached in 9 eyes. However, the protein deposited on soft contact lens was lysozyme which was unchanged by drinking. The amounts of cholesterol and methyl oleate after drinking were 85.5% (p=0.25) and 52.6% (p=0.002) of non-drinking's indicating some change of lipid deposit on soft contact lens by drinking. Conclusions: The results showed the composition of protein and lipid deposited on soft contact lens was changed due to drinking. Thus, it is suggested that wearing soft contact lens when drinking might be one of the reasons to feel discomfort.

• Journal of Life Science
• /
• v.26 no.10
• /
• pp.1163-1168
• /
• 2016

Plant growth-promoting rhizobacteria (PGPR) have gained worldwide importance and acceptance due to their agricultural benefits. These microorganisms are potential tools for sustainable agriculture, with effects on plant growth, biofertilization, induced systemic resistance, and biocontrol of plant pathogens. In this study, four different Pantoea species were isolated from field soil, and their plant growth-promoting characteristics were studied. Based on 16S rDNA gene sequencing analyses, the se were grouped into Pantoea ananatis, Pantoea citrea, Pantoea dispersa, Pantoea vagans and named as Pa1, Pc1, Pd1, Pv1, respectively. All of these strains have their ability for solubilization of insoluble phosphate depending on pH decrease at the range around pH 5 at 1days after inoculation and production of plant hormone indole acetic acid (IAA) with 85.3±16.3 μg/ml of Pa1, 183.9±16.8 μg/ml of Pc1, 28.8±17.3 μg/ml of Pd1 and 114.1±16.5 μg/ml of Pv1, respectively. Pa1, Pc1 and Pd1 also have high activity for production of gibberellin (GA₃) hormone with 331.1±19.2 μg/ml of Pa1, 288.5±16.8 μg/ml of Pc1, 309.2±18.2 μg/ml of Pd1, but Pv1 does not. Furthermore, all these species have significantly promoted the growth of the lettuce seedling plants at the range around 32~37% for fresh weight and 10~15% for shoot length enhancement, so that these microbe could be used as a potential bio-fertilizer agents.

• Journal of Life Science
• /
• v.32 no.11
• /
• pp.899-907
• /
• 2022

Quercetin is one of bio-flavonoids which are abundant in fruits and vegetables and has been reported to have various pharmacological potentials such as anti-oxidation, anti-inflammation, anti-cancer, and anti-virus effects. In the present study, the anti-inflammatory effects and its working molecular mecha- nism of quercetin were investigated in mouse macrophage RAW264.7 cells. Quercetin significantly inhibited nitric oxide (NO) production in a dose-dependent manner without affecting cell viability and decreased inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW264.7 cells. In addition, quercetin decreased phosphorylation of p38, JNK, and ERK, and inhibited phosphorylation of NF-κB p65 protein and its inhibitor IκBα indicating that quercetin has the anti-inflammatory effects via regulation of MAPKs and NF-κB signaling pathway. We also detected expression changes of four kinds of pro-inflammatory cytokine genes (CSF2, IL-1β, IL-6, and TNF-α) with quantitative real-time PCR. The results showed that quercetin decreased the expression of four pro-inflammatory genes in LPS-stimulated RAW264.7 cells. Overall, our results showed that quercetin effectively suppressed inflammation responses induced by LPS in RAW264.7 cells via regulating MAPK and NF-κB pathway and down-regulating the expression of pro-inflammatory cytokine genes.

• Analytical Science and Technology
• /
• v.8 no.1
• /
• pp.25-32
• /
• 1995

Determination method of trace thioglycolate has been studied by adsorptive stripping voltammetry. Copper(II)-thioglycolate complex is adsorbed at the hanging mercury drop electrode and stripped during cathodic scan. Electrolyte was used pH 6.5 phosphate and pH 9.5 borate buffer solutions. Optimal conditions were a copper(II) concentration $1{\times}10^{-4}M$, an adsorption accumulation potential -0.2V, an adsorption accumulation time 60 sec and a scan rate 20mV/sec. A detection limit of $1{\times}10^{-9}M$ thioglycolate was obtained. The method was applied to the determination of thioglycolate in cold wave fluids and depilating creams.