• The Journal of Korean Academy of Prosthodontics
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• v.48 no.2
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• pp.122-127
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• 2010

Purpose: The objective of this study was to investigate the effects of calcium phosphate coated titanium implant surface on bone response and implant stability at early stage of healing period of 3 weeks and later healing period of 6 weeks. Material and methods: A total of 24 machined, screw-shaped implants (Dentium Co., Ltd., Seoul, Korea) which dimensions were 3.3 mm in diameter and 5.0 mm in length, were used in this research. All implants (n = 24), made of commercially pure (grade IV) titanium, were divided into 2 groups. Twelve implants (n = 12) were machined without any surface modification (control). The test implants (n = 12) were anodized and coated with thin film (150nm) of calcium phosphate by electron-beam deposition. The implants were placed on the proximal surface of the rabbit tibiae. The bone to implant contact (BIC) ratios was evaluated after 3 and 6 weeks of implant insertion. Results: The BIC percentage of calcium phosphate coated implants ($70.8{\pm}18.9%$) was significantly higher than that of machined implants ($44.1{\pm}16.5%$) 3 weeks after implant insertion (P = 0.0264). However, there was no significant difference between the groups after 6 weeks of healing (P > .05). Conclusion: The histomorphometric evaluation of implant surface revealed that; 1. After 3 weeks early healing period, bone to implant contact (BIC) percentage of calcium phosphate coated implants (70.8%) was much greater than that of surface untreated machined implants (44.1%) with P = 0.0264. 2. After 6 weeks healing period, however, BIC percentage of calcium phosphate coated implants group (79.0%) was similar to the machined only implant group (78.6%). There was no statistical difference between two groups (P = 0.8074). 3. We found the significant deference between the control group and experimental group during the early healing period of 3 weeks. But no statistical difference was found between two groups during the later of 6 weeks.

• Development and Reproduction
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• v.2 no.2
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• pp.213-221
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• 1998

Intracytoplasmic sperm injection (ICSI) has been widely used to treat couples with infertility due to severely impaired sperm charateristics and for whom conventional in-vitro fertilization (IVF) had failed. The extent to which the morphology of the oocyte at the light microscopy level is related to the results of ICSI vis controversial. In this study, oocytes from 44 patients were reviewed. The ICSI procedure was recorded through CCD camera. The oocytes were divided into five groups according to the presence of cytoplasmic inclusions, the width of perivitelline space (PVS), the presence of cell debris in PVS, the status of first polar body and the flexibility of oolemma. The results showed that the fertilization rate and embryonic development were not associated with the morphological criteria of oocyte. The degeneraton rate of oocytes after ICSI was significantly higher (P<0.001) in the oocytes whose membranes were broken at the moment of insertion (17.7%) than the oocytes whose membranes were broken by aspiration of cytoplasm (1.6%). More oocytes with cytoplasmic inclusions (48.4% vs. 25.1%, p<0.001), wide PVS (35.2% vs. 19.0%, p<0.001), or cell debris in PVS (53.3% vs. 38.4%, p<0.05) were observed in patients with female factor infertility compared to patients with male factor infertility. These results .suggest that the fertilization rate and embryonic development after ICSI are not correlated with oocyte morphology based on the presence of cytoplasmic inclusions, size of PVS, the presence of cell debris in PVS and the status of polar body. And the degeneration rate of oocytes after ICSI was associated with the flexibility of oolemma.

• Molecules and Cells
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• v.27 no.5
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• pp.563-570
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• 2009

We previously isolated the OsCBT gene, which encodes a calmodulin (CaM)-binding protein, from a rice expression library constructed from fungal elicitor-treated rice suspension cells. In order to understand the function of OsCBT in rice, we isolated and characterized a T-DNA insertion mutant allele named oscbt-1. The oscbt-1 mutant exhibits reduced levels of OsCBT transcripts and no significant morphological changes compared to wild-type plant although the growth of the mutant is stunted. However, oscbt-1 mutants showed significant resistance to two major rice pathogens. The growth of the rice blast fungus Magnaporthe grisea, as well as the bacterial pathogen Xanthomonas oryzae pv. oryzae was significantly suppressed in oscbt-1 plants. Histochemical analysis indicated that the hypersensitive-response was induced in the oscbt-1 mutant in response to compatible strains of fungal pathogens. OsCBT expression was induced upon challenge with fungal elicitor. We also observed significant increase in the level of pathogenesis-related genes in the oscbt-1 mutant even under pathogen-free condition. Taken together, the results support an idea that OsCBT might act as a negative regulator on plant defense.

• Journal of Periodontal and Implant Science
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• v.30 no.3
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• pp.619-632
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• 2000

This clinical study was designed to determine the clinical and microbiological outcomes and safety of using minocycline loaded polycaprolactone strip for pericoronitis patients. 64 patients showing symptoms and signs of pericoronitis were enrolled according to the inclusion criteria in this double blind study. They were randomly assigned to two groups. 32 patients comprised control group and they received only polycaprolactone films in pericoronal spaces, and another 32 patients comprised experimental group and they received polycaprolactone films loaded with 30% minocycline. Informed consent was obtained from all the participants before beginning the study. At the initial visit, gingival index(GI), papillary bleeding index(PBI), amount of gingival crevicular fluid(GCF) were recorded, and microbiological sampling was done. Then, loaded or unloaded polycaprolactone film was inserted into the pericoronal spaces. No drug was prescribed excepting this film. After one week, clinical and microbiological exam was repeated. Presence of any side effects or inconveniences were checked. Chi-square test and t-test was performed to compare outcomes. At baseline, there were no significant differences in all the criteria between experimental group and control group. Experimental group showed significant improvement compared with control group both in GI(p<0.01) and PBI(p<0.01). The amount of GCF of the experimental group was significantly decreased compared with the control group(p<0.01) and baseline(p<0.01). In microbiological study, percentage of motile rod was prominently decreased in the experimental group. Also, aerobic(p<0.001), anaerobic(p<0.001) and black pigmented(p<0.01) bacteria were significantly decreased from the baseline. Furthermore, no side effects or inconveniences was reported in the experimental group. From this study, it was concluded that insertion of polycaprolactone film with 30% minocycline into the pericoronal spaces would be effective and safe treatment for pericoronitis.

• Journal of Genetic Medicine
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• v.2 no.1
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• pp.11-15
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• 1998

This paper reports 3 cases with 46,XX sex reversed male. Three 46,XX hypogonadal subjects showed complete sex reversal and had normal phallus and azoospermia. We studied them under clinical, cytogenetic and molecular aspects to find out the origin of the sex reversal. Patients had markedly elevated serum follicle-stimulating hormone (FSH) and lutenizing hormone (LH) and decreased or normal range of serum testosterone. The testicular volumes were small (3-8ml). Testicular biopsy showed Leydig cell hyperplasia and atrophy of seminiferous tubules. We obtained the results of normal 46,XX, and the presence of Y chromosome mosaicism was ruled out through XY dual fluorescent in situ hybridization (FISH). By using polymerase chain reaction (PCR), we amplified short arm (SRY, PABY, ZFY and DYS14), centromere (DYZ3), and heterochromatin (DYZ1) region of the Y chromosome. PCR amplification of DNA from these patients showed the presence of the sex-determining region of the Y chromosome (SRY) but didn't show the centromere and heterochromatin region sequence. The SRY gene was detected in all the three patients. Amplification patterns of the other regions were different in these patients; one had four amplified loci (PABY+, SRY+, ZFY+, DYS14+), another had two loci (SRY+, ZFY+) and the other had two loci (PABY+, SRY+). We have found that each patient's translocation elements had different breakpoints at upstream and downstream of the SRY gene region. We conclude that the testicular development in 46,XX male patients were due to insertion or translocation of SRY gene into X chromosome or autosomes.

• The Journal of Advanced Prosthodontics
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• v.1 no.3
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• pp.124-128
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• 2009

STATEMENT OF PROBLEM. How the ISQ values measured by $Osstell^{TM}$ and $Osstell^{TM}$ Mentor are related, and whether the ISQ values acquired from the two machines changes in accordance with changes in implant stability are not yet fully understood. PURPOSE. The aim of this study was to find out correlation between the ISQ values acquired from $Osstell^{TM}$ and $Osstell^{TM}$ Mentor, and to evaluate the clinical effectiveness and accuracy of two devices. MATERIAL AND METHODS. Sixty two implants were inserted into 47 patients, and their ISQ values were measured using $Osstell^{TM}$ and $Osstell^{TM}$ Mentor. In the first stage surgery, the ISQ values of forty four implants inserted into thirty five patients were measured. In the second stage surgery, the values of fifty implants inserted into thirty seven patients were measured. The values were analyzed to determine the difference between the mean ISQ values of $Osstell^{TM}$ and $Osstell^{TM}$ Mentor. In addition, the correlation between implants used in the first and second stage of surgery with regard to their types and areas of insertion were analyzed. The difference between the ISQ values of 32 implants in each patient during the first and second stage was analyzed. The statistical assessment was carried out using SPSS V. 12.0 for Win. (SPSS Inc., Chicago, USA). The Pearson correlation coefficient was used to examine the correlation between $Osstell^{TM}$ and $Osstell^{TM}$ Mentor in the first and second stages of surgery, whereas the difference between their ISQ values was evaluated using a paired t-test. RESULTS. In the first stage, the mean ISQ value for $Osstell^{TM}$ and $Osstell^{TM}$ Mentor was 70.84 and 75.09, respectively, showing a significant difference (P < .01). In the second stage, the mean ISQ value of $Osstell^{TM}$ and $Osstell^{TM}$ Mentor was 71.76 and 75.94, respectively, also showing a significant difference (P < .01). The difference between the ISQ values in patients in the first and the second stages was significant with both instruments. CONCLUSION. The significant difference in the values obtained using the $Osstell^{TM}$ and $Osstell^{TM}$ Mentor between the first and second stages of implant surgery indicates that these values can be a convenient and precise way for evaluating the implant stability in clinical practice.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.38 no.5
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• pp.276-283
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• 2012

Objectives: Curettage and enucleation are two of the most common procedures performed in oral and maxillofacial surgery units. To access a cystic lesion, the buccal cortical plate is removed. The no reposition (NR) group underwent surgery without repositioning the buccal cortical plate. The reposition (R) group underwent surgery with a repositioning of the buccal cortical plate. This study compared the two surgical procedures in terms of bone healing and complications. Materials and Methods: Patients who underwent curettage and enucleation surgery were enrolled in this study. Panoramic radiographs of the patients in both the NR group (n=26) and R group (n=34) were taken at the baseline and at 6, 12 and 24 months after surgery. The radiolucent area was calculated to evaluate bony healing in each radiograph. The complications were analyzed through a review of the medical records. Results: The correlation between bony healing and surgical approach was not significant in the 6th, 12th, and 24th month (P<0.05). The complication rate was not associated with gender, graft material, bone graft and drain insertion (P<0.05). On the other hand, the R group had a higher complication rate (35.3%) than the NR group (0%). The difference in the mean lesion size between the NR group ($37,024{\pm}3,617$ pixel) and R group ($92,863{\pm}15,931$ pixel) was significant (independent t test, P=0.004). Conclusion: Although the reposition method is chosen when the lesion size is large, it is associated with more complications. Indeed, infection, discomfort and recurrence of the lesion were the most common complications in the R group. Furthermore, the R method does not have a strong point in terms of bone healing compared to the NR method. Therefore, the R method cannot be considered an ideal approach and should be used in limited cases.

• Journal of Acupuncture Research
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• v.20 no.3
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• pp.194-208
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• 2003

Objective : Recently, many studies have showed the evidences of the effect of acupuncture treatment through scientific methods. We performed electro-acupuncture on Taixi(K3) and observed the change of brain activity using fMRI. Methods : To see the effect of electro-acupuncture stimulation on Taixi(K3), the experiment was carried out on twelve healthy volunteers, using the gradient echo sequence with the 3.0T whole-body fMRI system(ISOL). After the needle insertion on K3, 2 Hz of electric stimulation was given for 30 seconds, repeated five times, with 30 seconds' intervals. Result & Conclusions : Unilateral electro-acupuncture stimulation on K3 increased the brain activity in Brodman Area 8, 6, 9, 13, 21, 22, 38, 40, 43, 2, 5, 10, 20, 24, 32, 34, 37, 39, 41, 42. and decreased the brain activity in Brodman Area 9, 13, 38, 22, 31, 3, 6, 10, 21, 25, 29, 30. Group Averaged Brain activity induced by Unilateral Electro-acupuncture Stimulation on K3 was increased in Brodman Area 10, 22, 42, 43, 44 and was decreased inn Brodman Area 6 and pyrogen test of Bee venom. Bilateral electro-acupuncture stimulation on k3 increased the brain activity in Brodman Area 22, 3, 19, 4, 13, 17, 21, 41, 42, 2, 5, 6, 7, 18, 23, 29, 30, 31, 40 and decreased the brain activity in Brodman Area 18, 6, 39, 4, 13, 2, 10, 19, 36, 40, 45, 46, 47. Group Averaged Brain activity induced by Bilateral Electro-acupuncture Stimulation on K3 was increased in Brodman Area 22, 13, 4, 3, 29, 38, 43 and was decreased in Brodman Area 18, 3, 19, 22, 39. Motor Stimulation activated Brodmann Area 6, 43, 18.

• Journal of Korean Academy of Nursing Administration
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• v.11 no.4
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• pp.449-467
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• 2005

Purpose: This study aims to offer the fundamental data in order to cost the nursing service on the basis of the NIC and a close examination of the interventions that are contained in the health insurance cost list under the system of the current health insurance. Methods: The data is handled with the SPSS 10.0 program. The participants' general peculiarity is calculated in terms of the real number and the percentage, and the performing frequency of the nursing interventions is calculated in terms of the mean and the standard deviation. the correlation between the participants' general peculiarity and the performing frequency of the nursing interventions is analysed with t-test or one way ANOVA of SPSS. Results: In the performing frequency of the nursing interventions, the domain of "the physiological: basic" was the highest as 2.69${\pm}$1.21, the domain of "the behavioral" was the lowest as 2.11${\pm}$1.12. There were 50 core interventions in the medical unit, 48 in the surgical unit, 24 in the MICU and 33 in the SICU. The health insurance cost items contained commonly in the core interventions of each unit were 12, and the health insurance cost items except 12 items contained commonly in the core interventions of each unit were appeared 14 items in the medical unit, 6 in the surgical unit, 7 in the MICU and 2 in the SICU. The core interventions contained commonly in four units of the medical unit, the surgical unit, the MICU & the SICU are 18. And among these, the core interventions contained in the health insurance cost items are 10; pain management, hyperglycemia management, analgegic administration, medication administration: intravenous, oxygen therapy, pressure ulcer prevention, fluid management, fluide monitoring, intravenous(IV) insertion, intravenous(IV) therapy. As the result of the comparison & analysis between the core interventions of the NIC and the health insurance cost items, the core interventions contained in the health insurance cost list are 21(29 as the health insurance cost items). Conclusion: In the performing frequency of the nursing interventions, the domain of "the physiological: basic" is being performed most frequently, and in the performing frequency of the core interventions, the interventions of the domain of "the physiological: complex" is being performed most frequently. On the basis of these results, the writer hopes that the attempts to interlink the nursing interventions into the nursing cost by using of standard terms and the efforts to cost the nursing services would also be made in the future constantly.

• Proceedings of the KSPS conference
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• 2000.07a
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• pp.86-87
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• 2000

In this paper, we discuss why two different surface forms appear in loanwords for English and German /${\int}$/ In Korean, a vowel is inserted into loanwords if a consonant cannot be properly syllabified. Therefore, /${\int}$/ in some positions of loanwords trigger vowel insertion. Interestingly, /${\int}$/s in the onset cluster of English and German words were borrowed in Korean as Iful with the inserted vowel [u] whereas If Is in the coda position of English and German words were borrowed as Ifil with the inserted vowel [i]. For example, 'shrimp' is adopted as [${\int}urimphi$] whereas 'rush' is adopted as [$ra{\int}i$]. In this paper, we attempt to find out the phonetic reason for the distribution of the surface forms of /${\int}$/. We assume that since the formant frequency of [i] is higher than that of [u], the peak frequency of /${\int}$/ with the surface form of [${\int}$i] in loanwords may be higher than that of /${\int}$/ with the surface form of [${\int}u$]. We also assume that duration may be another factor for the distribution of [${\int}i$] and [${\int}u$]. Since /${\int}$/ and /u/ use lip rounding whereas /i/ doesn't, the duration for [${\int}i$] might be longer than that of [${\int}u$]. German supports our assumption. /${\int}$/ in the onset cluster is longer than /${\int}$/ in the coda position. It also has higher peak frequency than that of /${\int}$/ in the coda position. In loanwords, ${\int}$ in the onset cluster is borrowed as [${\int}u$] as in Spiegel whereas /${\int}$/ in the coda position is borrowed as [${\int}i$] as in Bosch. English, however, does not support our assumption. Peak frequency of [${\int}$] depends on the preceding vowel, not on its position in the syllable structure. If the preceding vowel is front, then the peak freuency of the following of the following /${\int}$/ is high but if the preceding vowel is back, than the peak frequency of the following /${\int}$/ is low. The peak frequency of /${\int}$/ in the onset cluster seems to be in between. As we assumed, however, the duration of /${\int}$/ in the coda position is longer than of /${\int}$/ in the onset cluster. With the mixed results, we question whether Koreans really hear two different xounds for /${\int}$/ in English words. For the future experiment, we would like to perform the perception tet for /${\int}$/ in English words.