• Applied Biological Chemistry
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• v.48 no.3
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• pp.222-227
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• 2005

To evaluate the antimutagenic activity of the specialty rices, a giant embryonic rice and a pigmented rice, we measured the inhibitory effect on the chemically induced mutagenesis in E. coli and V79 cultured cell system, as well as on DNA strand scission induced by oxidative damages in vitro. When the inhibitory activity to mitomycin C-induced mutagenesis using SOS chromotest in E. coli cell was measured, the activities decreased in the following order: germinated pigmented rice (40.4%) > germinated giant embryonic rice (37.1%) > pigmented rice (35.5%) > germinated brown rice (15.7%) > giant embryonic rice (14.0%) > brown rice (0.8%). The activities for inhibiting mitomycin C-induced DNA strand scission decreased in the order of pigmented rice > giant embryonic rice > germinated pigmented rice > germinated brown rice > brown rice > germinated giant embryonic rice. We also determined antimutagenic activities of the specialty rices using the suppressing effect on 6-TG resistant colony formation by 4-NQO in V79 cells as a mutagenicity index. The order of antimutagenicity was germinated giant embryonic rice (53.2%) > pigmented rice (40.0%) > brown rice (21.2%) > germinated brown rice (14.4%) > giant embryonic rice (0.23%); in contrast, germinated pigmented rice showed promoting effect on 4-NQO-induced mutagenesis.

• Journal of Life Science
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• v.29 no.10
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• pp.1120-1125
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• 2019

The short-lived nematode Caenorhabditis elegans has been used as a model organism for many studies, including lifespan extension. To screen common seaweeds for natural anti-aging agents, the lifespan of C. elegans (N2 wild-type strain) was measured by its hatch rate, growth rate, survival rate, chemotaxis, brood size, and egg-laying time after exposure to nematode growth medium (NGM) containing seaweed extracts. Approximately 30 animals synchronized at the first larval stage were incubated until they reached their adult stages before laying their eggs and were transferred to fresh NGM every 3 days. We also identified the major active compound from the seaweed by gas chromatography-mass spectrometry and tested its optimal dose for longevity. Of 13 common seaweed species, an ethanol extract of the brown seaweed Hizikia fusiformis showed the greatest effect on hatching, growth, and survival rates. The lifespan of C. elegans was significantly expanded 1.54-fold and 1.23-fold in the presence of the ethanol extract (0.05 mg/ml) and the main active component, fucosterol (0.05 mg/ml), respectively. Exposure to the ethanol extract also increased chemotaxis 1.13-fold, decreased brood size 0.74-fold, and shortened egg-laying time 0.96-fold. These results suggest that the aquaculturable H. fusiformis may be a promising source of a diet supplement to support health care.

• Journal of Embryo Transfer
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• v.20 no.2
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• pp.129-135
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• 2005

The present study examines the effects of kinds and concentrations of cryoprotectants, PVP and sucrose and trehalose on the survival rate of vitrified porcine embryos. 1. The developmental stages for the embryos used in vitrification were $245(23.0\%)$ for 2 cell stage, $256(24.1\%)$ for the blastocyst, $234(22.0\%)$ for the early blastocyst $221(20.8\%)$ from the expanded blastocyst and $107(10.1\%)$ from hatching blastocyst out of 1,063 embryos. 2 The survival rate of morula, early blastocyst and expanded blastocyst vitrification-thawed with EDT and EGS were $69.1\%,\;70.3\%,\;69.8\%\;and\;62.5\%,\;61.7\%,\;63.6\%$, respectively. The expanded blastocyst treated with EDS showed the highest survival rate compared with the other cryoprotectants. 3. The survival rate of early blastocyst, expanded blastocyst and hatching blastocyst vitrification-thawed with EDS diluted in $medium + 10\%$ FCS were $61.1\%,\;27.8\%,\;16.7\%$, respectively. This result were love. than the control of group $(92.3\%,\;71.2\%,\; 55.8\%)$. 4. The survival rate of embryos vitrified with EDS and EDT supplemented with $10\%\;and\;20\%$ PVP were $74.3\%,\;77.5\%\;and\;79.4\%,\;71.1\%$, respectively. The survival rate of vitrified embryos cultured for $24\~48$ hours were $37.1\%,\; 40.0\%\;and\;35.3\%,\;31.6\%$ which were significantly lower than that of non-cultured embryos. The survival rate of embryos vitrified with EDS and EDT supplemented between $10\%\;or\;20\%$ PVP did not have a significant difference. 5. The survival rate of embryos vitrification-thawed with EDS to morula, early blastocyst, expanded blastocyst and hatching blastocyst were $58.2\%,\; 36.4\%,\;14.5\%$ to morula, $62.5\%,\;45.8\%,\;20.8\%$ to early blastocyst, $74.1\%,\;61.1\%,\;29.6\%$ to expanded blastocyst and $60.0\%,\;40.0\%,\;14.0\%$ to hatching blastocyst.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.6
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• pp.2677-2684
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• 2011

This study was conducted to clarify relationships between BMI and the incidence of hypertension, hyperglycemia and hypercholesterolemia among health checkup examinees. The study sample consists of 3,402 adults aged 30s - 60s years, who underwent health check-up at a university hospital. during the Jan. 2007 to Dec. 2008.As a results, blood pressure level at the base line, ORs(95% CI) of hypertension compared with BMI category of 18.5-22.9(normal group) were 1.7(1.2-3.8) for obesity group I, and 2.9(1.7-6.2) for obesity group II. After adjusting for age and sex, ORs(95% CI) of hypertension compared with BMI category of normal group were 1.3(1.0-1.7) for obesity group I, 1.8(1.3-2.4) for obesity group II. Serum total cholesterol level at the base line, ORs(95% CI) of hypercholesterolemia compared with BMI category of normal group were 1.7(1.5-4.9) for obesity group I, and 2.8(1.8-5.3) for obesity group II. After adjusting for age and sex, ORs(95% CI) of hypercholesterolemia compared with BMI category of normal group were 1.5(1.1-1.9) for obesity group I, and 2.3(1.7-3.1) for obesity group II.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.347-347
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• 1994

효소억제제와 투과촉진제를 첨가하지 않은 경우 비강, 직장 및 질점막을 통한 YAGH니 투과정도를 검토한 결과 donor측 잔존률이 경시적으로 급격히 감소하여 24시간후각각 7.04, 4.21 및 51.34％ 잔존하였다. 또한 receptor측으로의 투과량는 비강점막의 경우 8시간대에 3.9%의 투과율을 기점으로 다시 분해되었으며 직장 점막에서는 최대 0.72% 로 거의 검출되지 않았다. 질점막의 경우에는 24시간대에 1.8％의 투과에 머물렀다. 한편 혼합효소억제제로 TM가 EDTA를 양측 셀에 첨가한 조건에서는 첨가되지 않은 경우와 비교하여 donor측에서의 YAGFM이 서서히 감소됨과 동시에 receptor cell로의 투과량이 다소증대되었다. 더욱 양측 셀에 혼합효소억제제를 넣고 donor cell에 투과촉진제를 첨가한 경우 점막투과성을 현저히 증대시켰으며 LPC를 사용한 경우 효소억제제만을 사용한 경우와 비교하여 비강에서 3.6배, 직장에서 3.2배 및 4.1배 투과 flux를 증가시켰다.

• Proceedings of the Korean Journal of Food and Nutrition Conference
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• 2000.12a
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• pp.54.2-54
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• 2000

일반식용의 참쑥과 강화지역 특산물인 강화약쑥의 첨가급식이 체성장 및 성분에 미치는 영향을 관찰하고자 89g 내외의 흰쥐(Sprague Dawley) 24두를 3개 동물군(대조군, 참쑥군 및 약쑥군)으로 나누어 4주간 해당 실험식이(대조식이, 5%참쑥식이 및 5%약쑥식이)로 급식하였다. 쑥의 일반영양 성분 함량은 참쑥 중 조단백질과 조회분은 약쑥보다 약 2%이상 높았으나 반대로 조지방은 약 2%낮았다. 또한 약쑥 중 칼슘은 참쑥보다 5.3배 높았으나 반대로 참쑥 중 망간, 아연, 철, 마그네슘은 2.8∼2.3배, 비타민A는 2.9배가 높았다. 체중 증가율과 식이효율은 참쑥군 과 약쑥군이 모두 대조군과 비슷하였다. 혈청 중 총단백질, albumin, 요소질소, creatinine, 뇨산, 총cholesterol, HDL-C, LDL-C, 당 함량과 amylase와 transaminase(GOT, GPT)의 활성이 참쑥군과 약쑥군 간에 유의성 있는 차이를 나타내지는 않았으나 LDH의 활성은 약쑥군이 대조군과 참쑥군에 비하여 감소(p<0.05)하였다.

• Horticultural Science & Technology
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• v.17 no.3
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• pp.341-343
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• 1999

Quality of 'Niitaka' pear fruit was evaluated by instrumental and sensory analysis in relation to storability. Fruits harvested at commercial maturity were stored in a common storage room or in a cold storage at $2^{\circ}C$. During storage, fruits were sorted by instrumental measurement of soluble solid content (SSC) and flesh firmness. Then, overall acceptability was evaluated by organoleptic test. Critical storage period was determined by sensory evaluation index for different storage methods. After 60 days of storage, eating quality was acceptable when flesh firmness was higher than $3.3kg/8mm{\emptyset}$. As for soluble solid contents, high eating quality was obtained when pear fruit contained soluble solids higher than $13.0^oBrix$. In 'Niitaka' pears, however, changes in soluble solid content seemed not to be an appropriate parameter to determine storability since SSC increased during both common and cold storage. Data of organoleptic test and postharvest changes in flesh firmness suggested that storability of 'Niitaka' pear fruit seemed to be 30 days in a common storage and 120 days in a refrigerated storage.

• Journal of the Korea Society of Computer and Information
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• v.28 no.3
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• pp.101-110
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• 2023

This study was conducted to identify factors influencing weight loss efforts in hypertensive patients with obesity in Korea using data from the 2016-2019 National Health and Nutrition Examination Survey. Weight perception, weight loss efforts, and weight control methods were investigated for 1,910 subjects. Data were analyzed using descriptive analysis, cross-tabulation analysis and logistic regression. Among obese hypertensive patients, 12.6% perceived their weight as normal. Weight loss efforts were 2.03 times (95% CI: 1.48 to 2.78) higher in people with overweight perception than those with normal weight perception, and 1.74 times (95% CI: 1.33 to 2.26) higher in women than in men. In addition, those with class 1 obesity were 1.50 times (95% CI: 0.85 to 2.65) higher than those with class 3 obesity, and those with class 2 obesity were 2.16 times (95% CI: 1.16 to 4.00) higher than those with class 3 obesity. These results suggest that weight management approaches for hypertensive patients with obesity should be individually designed according to weight perception, gender, and obesity class.

• Journal of Sericultural and Entomological Science
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• v.35 no.1
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• pp.7-10
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• 1993

Mulberry grafting season in normally a one month period starting at the end of March. Such a short period creates a shortage of qualified grafters and inflates wages. Pine tree sawdust was tested as a medium in which to preserve graftages. Sawdust was moistened to two ratios of water to dry weight of sawdust (1.5 and 2.0). Scions were either grafted the same day they were collected or first incubated for 10days at 15$^{\circ}C$. Grafting period could be extended to the middle of February under 1.5 times moisture content and fresh scions. Preservation of scions increased water content, T-N, P2O5, CaO and inositol, whereas decreased total C, glucose and fructose. With no preservation total C decreased, inositol increased slightly early March followed sharp decrease, and sucrose increased with time.

• Korean Journal of Medicinal Crop Science
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• v.13 no.1
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• pp.57-62
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• 2005

Salt-tolerant transgenic Panax ginseng plants were produced by introducing the SAL1 geue (3'(2'), 5'-bis-phosphate nucleotidase) that confers tolerance to the salts through Agrobacterium tumefaciens co-cultivation. Cotyledon explants of immature ginseng zygotic embryos cultured on Murashige and Skoog medium lacking growth regulators formed somatic embryos directly with below 10%, but the 74% tranformation rate were observed at the treatment of phytohormone with 1.0 mg/l 2,4-D and 0.5 mg/l kinetin. Somatic embryos were initially cultured on MS medium supplemented with 250 mg/l cefotaxime for 3 weeks and subsequently subcultured five times to a medium containing 100 mg/l kanamycin and 250 mg/l cefotaxime. Upon development into the cotyledonary stage, these somatic embryos were transferred to on the medium containing 50 mg/l kanamycin and 10 mg/l gibberellic acid to induce germination and strong selection. Integration of the transgene into the plants was confirmed by polymerase chain reaction with specific primers. The ginseng transformants with well-developed shoots and roots were successfully acclimatized in a greenhouse when they were planted in soil.